Genomic and epidemiological evidence of a dominant panton-valentine leucocidin-positive methicillin resistant staphylococcus aureus lineage in Sri Lanka and presence among isolates from the United Kingdom and Australia

Objective: To undertake the first detailed genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Sri Lanka. Methods: A prospective observational study was performed on 94 MRSA isolates collected over a 4 months period from the Anuradhapura Teaching Hospital, Sri Lanka. S...

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Autores:
McTavish, Sharla M.
Snow, Sarah J.
Cook, Ellie C.
Pichon, Bruno
Coleman, Sarah
Coombs, Geoffrey W.
Pang, Stanley
Arias, César A.
Díaz, Lorena
Boldock, Emma
Davies, Steve
Udukala, Mangala
Kearns, Angela Marie
Siribaddana, Sisira
De Silva, Thushan I.
Tipo de recurso:
Fecha de publicación:
2019
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/1853
Acceso en línea:
http://hdl.handle.net/20.500.12495/1853
https://dx.doi.org/10.3389%2Ffcimb.2019.00123
Palabra clave:
Staphylococcus aureus resistente a meticilina
Leucocidinas
Código de barras del ADN taxonómico
Whole genome sequencing
CC5
Sri Lanka
Rights
License
Attribution 4.0 International
Description
Summary:Objective: To undertake the first detailed genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Sri Lanka. Methods: A prospective observational study was performed on 94 MRSA isolates collected over a 4 months period from the Anuradhapura Teaching Hospital, Sri Lanka. Screening for mecA, mecC, and the Panton-Valentine leucocidin (PVL)-associated lukS-PV/lukF-PV genes and molecular characterization by spa typing was undertaken. Whole genome sequencing (WGS) and phylogenetic analysis was performed on selected multilocus sequence type (MLST) clonal complex 5 (CC5) isolates from Sri Lanka, England, Australia, and Argentina. Results: All 94 MRSA harbored the mecA gene. Nineteen spa types belonging to nine MLST clonal complexes were identified. Where origin of the sample was recorded, most isolates were from skin and soft tissue infections (70/91; 76.9%), with fewer causing bacteremia (16/91; 17.6%), empyema (3/91; 3.3%) and osteomyelitis (2/91; 2.2%). Sixty two (65.9%) isolates were PVL positive with the majority (56 isolates; 90.3%) belonging to a dominant CC5 lineage. This lineage, PVL-positive ST5-MRSA-IVc, was associated with both community and hospital-onset infections. Based on WGS, representative PVL-positive ST5-MRSA-IVc isolates from Sri Lanka, England and Australia formed a single phylogenetic clade, suggesting wide geographical circulation. Conclusions: We present the most detailed genomic analysis of MRSA isolated in Sri Lanka to date. The analysis identified a PVL-positive ST5-MRSA-IVc that is prevalent among MRSA causing clinical infections in Sri Lanka. Furthermore, this clone was also found among isolates from the United Kingdom and Australia.