Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests
Daptomycin has become a mainstay therapy for the treatment of serious vancomycin-resistant Enterococcus faecium infections. However, concern exists that current testing methods do not accurately predict the clinical success of daptomycin therapy. We evaluated a collection of 40 isolates of E. faeciu...
- Autores:
-
Schuetz, Audrey
Campeau, Shelley
Arias, Cesar A
Kohner, Peggy
Dien Bard, Jennifer
Hemarajata, Peera
Humphries, Romney M.
- Tipo de recurso:
- Fecha de publicación:
- 2020
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/2321
- Palabra clave:
- Enterococcus faecium
Ácido pirúvico
Daptomicina
Etest
MIC test
Broth microdilution
Daptomycin
- Rights
- License
- Acceso cerrado
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Repositorio U. El Bosque |
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|
dc.title.spa.fl_str_mv |
Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests |
dc.title.translated.none.fl_str_mv |
Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests |
title |
Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests |
spellingShingle |
Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests Enterococcus faecium Ácido pirúvico Daptomicina Etest MIC test Broth microdilution Daptomycin |
title_short |
Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests |
title_full |
Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests |
title_fullStr |
Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests |
title_full_unstemmed |
Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests |
title_sort |
Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests |
dc.creator.fl_str_mv |
Schuetz, Audrey Campeau, Shelley Arias, Cesar A Kohner, Peggy Dien Bard, Jennifer Hemarajata, Peera Humphries, Romney M. |
dc.contributor.author.none.fl_str_mv |
Schuetz, Audrey Campeau, Shelley Arias, Cesar A Kohner, Peggy Dien Bard, Jennifer Hemarajata, Peera Humphries, Romney M. |
dc.subject.decs.spa.fl_str_mv |
Enterococcus faecium Ácido pirúvico Daptomicina |
topic |
Enterococcus faecium Ácido pirúvico Daptomicina Etest MIC test Broth microdilution Daptomycin |
dc.subject.keywords.spa.fl_str_mv |
Etest MIC test Broth microdilution Daptomycin |
description |
Daptomycin has become a mainstay therapy for the treatment of serious vancomycin-resistant Enterococcus faecium infections. However, concern exists that current testing methods do not accurately predict the clinical success of daptomycin therapy. We evaluated a collection of 40 isolates of E. faecium across three centers by reference broth microdilution (BMD), and two gradient strips, to determine the precision of daptomycin MICs by these methods and the correlation of daptomycin MIC testing with mutations in the liaFSR system, one of the primary daptomycin resistance mechanisms among the enterococci. Daptomycin MICs spanned 3-log2 dilutions by BMD for 60.0% of isolates, 17.5% spanned 4 dilutions, 2.5% spanned 5 dilutions, and 20.0% spanned 6 or more dilutions. Fifteen isolates had MICs interpreted as susceptible by some tests and nonsusceptible by others. Neither BMD nor gradient diffusion tests could reliably differentiate isolates with or without mutations in liaFSR, resulting in a 59.8% very major error rate compared to determination of genotype by BMD, 63.5% by Etest, and 68.5% by MIC test strip. Imprecision in daptomycin MIC determination for E. faecium make establishment of a revised breakpoint challenging. Clinicians should be aware of this testing variability when making treatment decisions for patients with serious infections caused by this organism. |
publishDate |
2020 |
dc.date.accessioned.none.fl_str_mv |
2020-04-22T16:42:22Z |
dc.date.available.none.fl_str_mv |
2020-04-22T16:42:22Z |
dc.date.issued.none.fl_str_mv |
2020 |
dc.type.spa.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.local.spa.fl_str_mv |
artículo |
dc.identifier.issn.none.fl_str_mv |
1098-6596 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/2321 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1128/AAC.00745-18 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
repourl:https://repositorio.unbosque.edu.co |
identifier_str_mv |
1098-6596 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque repourl:https://repositorio.unbosque.edu.co |
url |
http://hdl.handle.net/20.500.12495/2321 https://doi.org/10.1128/AAC.00745-18 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.uri.none.fl_str_mv |
https://aac.asm.org/content/62/9/e00745-18.full |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.local.spa.fl_str_mv |
Acceso cerrado |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf313 |
dc.rights.creativecommons.none.fl_str_mv |
2018 |
rights_invalid_str_mv |
Acceso cerrado http://purl.org/coar/access_right/c_abf313 2018 http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
American Society for Microbiology |
dc.publisher.journal.spa.fl_str_mv |
Antimicrobial Agents and Chemotherapy Antimicrobial agents and chemotherapy |
institution |
Universidad El Bosque |
bitstream.url.fl_str_mv |
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Repositorio Institucional Universidad El Bosque |
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1814100692789362688 |
spelling |
Schuetz, AudreyCampeau, ShelleyArias, Cesar AKohner, PeggyDien Bard, JenniferHemarajata, PeeraHumphries, Romney M.2020-04-22T16:42:22Z2020-04-22T16:42:22Z20201098-6596http://hdl.handle.net/20.500.12495/2321https://doi.org/10.1128/AAC.00745-18instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengAmerican Society for MicrobiologyAntimicrobial Agents and ChemotherapyAntimicrobial agents and chemotherapyhttps://aac.asm.org/content/62/9/e00745-18.fullVariability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion testsVariability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion testsarticleartículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Enterococcus faeciumÁcido pirúvicoDaptomicinaEtestMIC testBroth microdilutionDaptomycinDaptomycin has become a mainstay therapy for the treatment of serious vancomycin-resistant Enterococcus faecium infections. However, concern exists that current testing methods do not accurately predict the clinical success of daptomycin therapy. We evaluated a collection of 40 isolates of E. faecium across three centers by reference broth microdilution (BMD), and two gradient strips, to determine the precision of daptomycin MICs by these methods and the correlation of daptomycin MIC testing with mutations in the liaFSR system, one of the primary daptomycin resistance mechanisms among the enterococci. Daptomycin MICs spanned 3-log2 dilutions by BMD for 60.0% of isolates, 17.5% spanned 4 dilutions, 2.5% spanned 5 dilutions, and 20.0% spanned 6 or more dilutions. Fifteen isolates had MICs interpreted as susceptible by some tests and nonsusceptible by others. Neither BMD nor gradient diffusion tests could reliably differentiate isolates with or without mutations in liaFSR, resulting in a 59.8% very major error rate compared to determination of genotype by BMD, 63.5% by Etest, and 68.5% by MIC test strip. Imprecision in daptomycin MIC determination for E. faecium make establishment of a revised breakpoint challenging. Clinicians should be aware of this testing variability when making treatment decisions for patients with serious infections caused by this organism.Acceso cerradohttp://purl.org/coar/access_right/c_abf3132018http://purl.org/coar/access_right/c_abf2THUMBNAILCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdf.jpgCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/cf60c896-105c-4457-81a4-2021b800ce4d/download7210a811635d1799e7c05fee5d259be7MD53ORIGINALCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdfCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdfapplication/pdf653854https://repositorio.unbosque.edu.co/bitstreams/bc5423d0-71bd-4cbf-b191-6b8d9300ebe6/download4f14d0acae686c8d2297d3d8441f7aa9MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/a12b7fbc-d4cf-43b4-a427-1be5e414c35e/download8a4605be74aa9ea9d79846c1fba20a33MD52TEXTCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdf.txtCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdf.txtExtracted texttext/plain30178https://repositorio.unbosque.edu.co/bitstreams/fe689109-a78a-4cdf-adbf-293a2a743745/downloadb02d37507e68498d2f6377282d37b06aMD5420.500.12495/2321oai:repositorio.unbosque.edu.co:20.500.12495/23212024-02-06 22:36:02.584restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |