Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests

Daptomycin has become a mainstay therapy for the treatment of serious vancomycin-resistant Enterococcus faecium infections. However, concern exists that current testing methods do not accurately predict the clinical success of daptomycin therapy. We evaluated a collection of 40 isolates of E. faeciu...

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Autores:
Schuetz, Audrey
Campeau, Shelley
Arias, Cesar A
Kohner, Peggy
Dien Bard, Jennifer
Hemarajata, Peera
Humphries, Romney M.
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/2321
Acceso en línea:
http://hdl.handle.net/20.500.12495/2321
https://doi.org/10.1128/AAC.00745-18
Palabra clave:
Enterococcus faecium
Ácido pirúvico
Daptomicina
Etest
MIC test
Broth microdilution
Daptomycin
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License
Acceso cerrado
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oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/2321
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network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.spa.fl_str_mv Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests
dc.title.translated.none.fl_str_mv Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests
title Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests
spellingShingle Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests
Enterococcus faecium
Ácido pirúvico
Daptomicina
Etest
MIC test
Broth microdilution
Daptomycin
title_short Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests
title_full Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests
title_fullStr Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests
title_full_unstemmed Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests
title_sort Variability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion tests
dc.creator.fl_str_mv Schuetz, Audrey
Campeau, Shelley
Arias, Cesar A
Kohner, Peggy
Dien Bard, Jennifer
Hemarajata, Peera
Humphries, Romney M.
dc.contributor.author.none.fl_str_mv Schuetz, Audrey
Campeau, Shelley
Arias, Cesar A
Kohner, Peggy
Dien Bard, Jennifer
Hemarajata, Peera
Humphries, Romney M.
dc.subject.decs.spa.fl_str_mv Enterococcus faecium
Ácido pirúvico
Daptomicina
topic Enterococcus faecium
Ácido pirúvico
Daptomicina
Etest
MIC test
Broth microdilution
Daptomycin
dc.subject.keywords.spa.fl_str_mv Etest
MIC test
Broth microdilution
Daptomycin
description Daptomycin has become a mainstay therapy for the treatment of serious vancomycin-resistant Enterococcus faecium infections. However, concern exists that current testing methods do not accurately predict the clinical success of daptomycin therapy. We evaluated a collection of 40 isolates of E. faecium across three centers by reference broth microdilution (BMD), and two gradient strips, to determine the precision of daptomycin MICs by these methods and the correlation of daptomycin MIC testing with mutations in the liaFSR system, one of the primary daptomycin resistance mechanisms among the enterococci. Daptomycin MICs spanned 3-log2 dilutions by BMD for 60.0% of isolates, 17.5% spanned 4 dilutions, 2.5% spanned 5 dilutions, and 20.0% spanned 6 or more dilutions. Fifteen isolates had MICs interpreted as susceptible by some tests and nonsusceptible by others. Neither BMD nor gradient diffusion tests could reliably differentiate isolates with or without mutations in liaFSR, resulting in a 59.8% very major error rate compared to determination of genotype by BMD, 63.5% by Etest, and 68.5% by MIC test strip. Imprecision in daptomycin MIC determination for E. faecium make establishment of a revised breakpoint challenging. Clinicians should be aware of this testing variability when making treatment decisions for patients with serious infections caused by this organism.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-04-22T16:42:22Z
dc.date.available.none.fl_str_mv 2020-04-22T16:42:22Z
dc.date.issued.none.fl_str_mv 2020
dc.type.spa.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.local.spa.fl_str_mv artículo
dc.identifier.issn.none.fl_str_mv 1098-6596
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/2321
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1128/AAC.00745-18
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv repourl:https://repositorio.unbosque.edu.co
identifier_str_mv 1098-6596
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
repourl:https://repositorio.unbosque.edu.co
url http://hdl.handle.net/20.500.12495/2321
https://doi.org/10.1128/AAC.00745-18
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv https://aac.asm.org/content/62/9/e00745-18.full
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.local.spa.fl_str_mv Acceso cerrado
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf313
dc.rights.creativecommons.none.fl_str_mv 2018
rights_invalid_str_mv Acceso cerrado
http://purl.org/coar/access_right/c_abf313
2018
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv American Society for Microbiology
dc.publisher.journal.spa.fl_str_mv Antimicrobial Agents and Chemotherapy
Antimicrobial agents and chemotherapy
institution Universidad El Bosque
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spelling Schuetz, AudreyCampeau, ShelleyArias, Cesar AKohner, PeggyDien Bard, JenniferHemarajata, PeeraHumphries, Romney M.2020-04-22T16:42:22Z2020-04-22T16:42:22Z20201098-6596http://hdl.handle.net/20.500.12495/2321https://doi.org/10.1128/AAC.00745-18instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengAmerican Society for MicrobiologyAntimicrobial Agents and ChemotherapyAntimicrobial agents and chemotherapyhttps://aac.asm.org/content/62/9/e00745-18.fullVariability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion testsVariability of daptomycin MIC values for enterococcus faecium when measured by reference broth microdilution and gradient diffusion testsarticleartículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Enterococcus faeciumÁcido pirúvicoDaptomicinaEtestMIC testBroth microdilutionDaptomycinDaptomycin has become a mainstay therapy for the treatment of serious vancomycin-resistant Enterococcus faecium infections. However, concern exists that current testing methods do not accurately predict the clinical success of daptomycin therapy. We evaluated a collection of 40 isolates of E. faecium across three centers by reference broth microdilution (BMD), and two gradient strips, to determine the precision of daptomycin MICs by these methods and the correlation of daptomycin MIC testing with mutations in the liaFSR system, one of the primary daptomycin resistance mechanisms among the enterococci. Daptomycin MICs spanned 3-log2 dilutions by BMD for 60.0% of isolates, 17.5% spanned 4 dilutions, 2.5% spanned 5 dilutions, and 20.0% spanned 6 or more dilutions. Fifteen isolates had MICs interpreted as susceptible by some tests and nonsusceptible by others. Neither BMD nor gradient diffusion tests could reliably differentiate isolates with or without mutations in liaFSR, resulting in a 59.8% very major error rate compared to determination of genotype by BMD, 63.5% by Etest, and 68.5% by MIC test strip. Imprecision in daptomycin MIC determination for E. faecium make establishment of a revised breakpoint challenging. Clinicians should be aware of this testing variability when making treatment decisions for patients with serious infections caused by this organism.Acceso cerradohttp://purl.org/coar/access_right/c_abf3132018http://purl.org/coar/access_right/c_abf2THUMBNAILCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdf.jpgCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/cf60c896-105c-4457-81a4-2021b800ce4d/download7210a811635d1799e7c05fee5d259be7MD53ORIGINALCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdfCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdfapplication/pdf653854https://repositorio.unbosque.edu.co/bitstreams/bc5423d0-71bd-4cbf-b191-6b8d9300ebe6/download4f14d0acae686c8d2297d3d8441f7aa9MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/a12b7fbc-d4cf-43b4-a427-1be5e414c35e/download8a4605be74aa9ea9d79846c1fba20a33MD52TEXTCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdf.txtCampeau S.A., Schuetz A.N., Kohner P., Arias C.A., Hemarajata P., Bard J.D., Humphries R.M._2018.pdf.txtExtracted texttext/plain30178https://repositorio.unbosque.edu.co/bitstreams/fe689109-a78a-4cdf-adbf-293a2a743745/downloadb02d37507e68498d2f6377282d37b06aMD5420.500.12495/2321oai:repositorio.unbosque.edu.co:20.500.12495/23212024-02-06 22:36:02.584restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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