Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone
Background Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms in...
- Autores:
-
Castellanos, Jaime
Abril Riaño, Deisy Julieth
Moncayo-Ortiz, José Ignacio
Corredor Rozo, Zayda Lorena
Reyes, Niradiz
Tovar, Catalina
Sánchez, Héctor Fabio
Guaca-González, Yina Marcela
Llanos-Uribe, Carmen Elisa
Olarte, Narda María
Vanegas, Natasha
Escobar-Pérez, Javier
Castro Cardozo, Betsy Esperanza
Marquez-Ortiz, Ricaurte Alejandro
- Tipo de recurso:
- Fecha de publicación:
- 2019
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/1571
- Palabra clave:
- Genética
Bacterias aerobias gramnegativas
Infecciones por pseudomonas
Carbapenémicos
bla KPC-2
Pseudomonas aeruginosa
Carbapenems
ST235
- Rights
- License
- Attribution 4.0 International
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|
dc.title.spa.fl_str_mv |
Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone |
title |
Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone |
spellingShingle |
Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone Genética Bacterias aerobias gramnegativas Infecciones por pseudomonas Carbapenémicos bla KPC-2 Pseudomonas aeruginosa Carbapenems ST235 |
title_short |
Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone |
title_full |
Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone |
title_fullStr |
Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone |
title_full_unstemmed |
Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone |
title_sort |
Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone |
dc.creator.fl_str_mv |
Castellanos, Jaime Abril Riaño, Deisy Julieth Moncayo-Ortiz, José Ignacio Corredor Rozo, Zayda Lorena Reyes, Niradiz Tovar, Catalina Sánchez, Héctor Fabio Guaca-González, Yina Marcela Llanos-Uribe, Carmen Elisa Olarte, Narda María Vanegas, Natasha Escobar-Pérez, Javier Castro Cardozo, Betsy Esperanza Marquez-Ortiz, Ricaurte Alejandro |
dc.contributor.author.none.fl_str_mv |
Castellanos, Jaime Abril Riaño, Deisy Julieth Moncayo-Ortiz, José Ignacio Corredor Rozo, Zayda Lorena Reyes, Niradiz Tovar, Catalina Sánchez, Héctor Fabio Guaca-González, Yina Marcela Llanos-Uribe, Carmen Elisa Olarte, Narda María Vanegas, Natasha Escobar-Pérez, Javier Castro Cardozo, Betsy Esperanza Marquez-Ortiz, Ricaurte Alejandro |
dc.contributor.orcid.none.fl_str_mv |
Castellanos, Jaime [0000-0003-1596-8383] Escobar-Pérez, Javier [0000-0002-0432-6978] Abril Riaño, Deisy Julieth [0000-0002-6686-6283] |
dc.subject.decs.spa.fl_str_mv |
Genética Bacterias aerobias gramnegativas Infecciones por pseudomonas Carbapenémicos |
topic |
Genética Bacterias aerobias gramnegativas Infecciones por pseudomonas Carbapenémicos bla KPC-2 Pseudomonas aeruginosa Carbapenems ST235 |
dc.subject.keywords.spa.fl_str_mv |
bla KPC-2 Pseudomonas aeruginosa Carbapenems ST235 |
description |
Background Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in blaKPC-2-positive P. aeruginosa ST235 in Colombia. Results In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the blaVIM and blaKPC-2 genes, respectively. The four blaKPC-2-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2″)-Ia, two Tn402-like with ant(3″)-Ia and blaOXA-2 and two Tn4401b with blaKPC-2. All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring blaKPC-2 were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background. Conclusion This is the first report of a double Tn4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism. |
publishDate |
2019 |
dc.date.accessioned.none.fl_str_mv |
2019-07-29T15:01:24Z |
dc.date.available.none.fl_str_mv |
2019-07-29T15:01:24Z |
dc.date.issued.none.fl_str_mv |
2019 |
dc.type.spa.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.local.spa.fl_str_mv |
artículo |
dc.identifier.issn.none.fl_str_mv |
1471-2180 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/1571 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1186/s12866-019-1418-6 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
repourl:https://repositorio.unbosque.edu.co |
identifier_str_mv |
1471-2180 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque repourl:https://repositorio.unbosque.edu.co |
url |
http://hdl.handle.net/20.500.12495/1571 https://doi.org/10.1186/s12866-019-1418-6 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
BMC Microbiology, 1471-2180, Vol.19, Num. 45, 2019 p.1-12 |
dc.relation.uri.none.fl_str_mv |
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1418-6 |
dc.rights.*.fl_str_mv |
Attribution 4.0 International |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
dc.rights.local.spa.fl_str_mv |
Acceso abierto |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf69 |
dc.rights.creativecommons.none.fl_str_mv |
2019 |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ Acceso abierto http://purl.org/coar/access_right/c_abf69 2019 http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Biomed Central |
dc.publisher.journal.spa.fl_str_mv |
BMC Microbiology |
institution |
Universidad El Bosque |
bitstream.url.fl_str_mv |
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Castellanos, JaimeAbril Riaño, Deisy JuliethMoncayo-Ortiz, José IgnacioCorredor Rozo, Zayda LorenaReyes, NiradizTovar, CatalinaSánchez, Héctor FabioGuaca-González, Yina MarcelaLlanos-Uribe, Carmen ElisaOlarte, Narda MaríaVanegas, NatashaEscobar-Pérez, JavierCastro Cardozo, Betsy EsperanzaMarquez-Ortiz, Ricaurte AlejandroCastellanos, Jaime [0000-0003-1596-8383]Escobar-Pérez, Javier [0000-0002-0432-6978]Abril Riaño, Deisy Julieth [0000-0002-6686-6283]2019-07-29T15:01:24Z2019-07-29T15:01:24Z20191471-2180http://hdl.handle.net/20.500.12495/1571https://doi.org/10.1186/s12866-019-1418-6instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengBiomed CentralBMC MicrobiologyBMC Microbiology, 1471-2180, Vol.19, Num. 45, 2019 p.1-12https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1418-6Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Acceso abiertohttp://purl.org/coar/access_right/c_abf692019http://purl.org/coar/access_right/c_abf2Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clonearticleartículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501GenéticaBacterias aerobias gramnegativasInfecciones por pseudomonasCarbapenémicosbla KPC-2Pseudomonas aeruginosaCarbapenemsST235Background Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in blaKPC-2-positive P. aeruginosa ST235 in Colombia. Results In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the blaVIM and blaKPC-2 genes, respectively. The four blaKPC-2-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2″)-Ia, two Tn402-like with ant(3″)-Ia and blaOXA-2 and two Tn4401b with blaKPC-2. All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring blaKPC-2 were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background. Conclusion This is the first report of a double Tn4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism.ORIGINALAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdfAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdfapplication/pdf3663813http://18.204.144.38/bitstreams/0cc7b9e3-1078-4b11-8a48-973d798c72d3/download1422d735975954ed4b8d26dc1803f7f4MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8908http://18.204.144.38/bitstreams/69e3770d-2022-4497-8db3-b5b3b62851b4/download0175ea4a2d4caec4bbcc37e300941108MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://18.204.144.38/bitstreams/dd5836ec-e513-46cc-9892-42c684791e07/download8a4605be74aa9ea9d79846c1fba20a33MD53THUMBNAILAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdf.jpgAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdf.jpgIM Thumbnailimage/jpeg10349http://18.204.144.38/bitstreams/f300b825-de78-43e2-bc02-460e91bded27/download4262d8a37bd20039ccfa81a6eb248c97MD54TEXTAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdf.txtAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdf.txtExtracted texttext/plain54131http://18.204.144.38/bitstreams/8429a97c-0d3c-4776-b8a3-3d04eb48d10d/download25b4ca2b3b81f935038632e3c14b23c7MD5520.500.12495/1571oai:18.204.144.38:20.500.12495/15712024-02-06 22:23:12.114http://creativecommons.org/licenses/by/4.0/Attribution 4.0 Internationalopen.accesshttp://18.204.144.38DSpace Pre-instalado Biteca S.A.Sbibliotecas@biteca.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 |