Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone

Background Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms in...

Full description

Autores:
Castellanos, Jaime
Abril Riaño, Deisy Julieth
Moncayo-Ortiz, José Ignacio
Corredor Rozo, Zayda Lorena
Reyes, Niradiz
Tovar, Catalina
Sánchez, Héctor Fabio
Guaca-González, Yina Marcela
Llanos-Uribe, Carmen Elisa
Olarte, Narda María
Vanegas, Natasha
Escobar-Pérez, Javier
Castro Cardozo, Betsy Esperanza
Marquez-Ortiz, Ricaurte Alejandro
Tipo de recurso:
Fecha de publicación:
2019
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/1571
Acceso en línea:
http://hdl.handle.net/20.500.12495/1571
https://doi.org/10.1186/s12866-019-1418-6
Palabra clave:
Genética
Bacterias aerobias gramnegativas
Infecciones por pseudomonas
Carbapenémicos
bla KPC-2
Pseudomonas aeruginosa
Carbapenems
ST235
Rights
License
Attribution 4.0 International
id UNBOSQUE2_c9a2e3764f1202e313c075ef1da5b988
oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/1571
network_acronym_str UNBOSQUE2
network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.spa.fl_str_mv Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone
title Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone
spellingShingle Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone
Genética
Bacterias aerobias gramnegativas
Infecciones por pseudomonas
Carbapenémicos
bla KPC-2
Pseudomonas aeruginosa
Carbapenems
ST235
title_short Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone
title_full Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone
title_fullStr Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone
title_full_unstemmed Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone
title_sort Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone
dc.creator.fl_str_mv Castellanos, Jaime
Abril Riaño, Deisy Julieth
Moncayo-Ortiz, José Ignacio
Corredor Rozo, Zayda Lorena
Reyes, Niradiz
Tovar, Catalina
Sánchez, Héctor Fabio
Guaca-González, Yina Marcela
Llanos-Uribe, Carmen Elisa
Olarte, Narda María
Vanegas, Natasha
Escobar-Pérez, Javier
Castro Cardozo, Betsy Esperanza
Marquez-Ortiz, Ricaurte Alejandro
dc.contributor.author.none.fl_str_mv Castellanos, Jaime
Abril Riaño, Deisy Julieth
Moncayo-Ortiz, José Ignacio
Corredor Rozo, Zayda Lorena
Reyes, Niradiz
Tovar, Catalina
Sánchez, Héctor Fabio
Guaca-González, Yina Marcela
Llanos-Uribe, Carmen Elisa
Olarte, Narda María
Vanegas, Natasha
Escobar-Pérez, Javier
Castro Cardozo, Betsy Esperanza
Marquez-Ortiz, Ricaurte Alejandro
dc.contributor.orcid.none.fl_str_mv Castellanos, Jaime [0000-0003-1596-8383]
Escobar-Pérez, Javier [0000-0002-0432-6978]
Abril Riaño, Deisy Julieth [0000-0002-6686-6283]
dc.subject.decs.spa.fl_str_mv Genética
Bacterias aerobias gramnegativas
Infecciones por pseudomonas
Carbapenémicos
topic Genética
Bacterias aerobias gramnegativas
Infecciones por pseudomonas
Carbapenémicos
bla KPC-2
Pseudomonas aeruginosa
Carbapenems
ST235
dc.subject.keywords.spa.fl_str_mv bla KPC-2
Pseudomonas aeruginosa
Carbapenems
ST235
description Background Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in blaKPC-2-positive P. aeruginosa ST235 in Colombia. Results In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the blaVIM and blaKPC-2 genes, respectively. The four blaKPC-2-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2″)-Ia, two Tn402-like with ant(3″)-Ia and blaOXA-2 and two Tn4401b with blaKPC-2. All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring blaKPC-2 were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background. Conclusion This is the first report of a double Tn4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism.
publishDate 2019
dc.date.accessioned.none.fl_str_mv 2019-07-29T15:01:24Z
dc.date.available.none.fl_str_mv 2019-07-29T15:01:24Z
dc.date.issued.none.fl_str_mv 2019
dc.type.spa.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.local.spa.fl_str_mv artículo
dc.identifier.issn.none.fl_str_mv 1471-2180
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/1571
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1186/s12866-019-1418-6
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv repourl:https://repositorio.unbosque.edu.co
identifier_str_mv 1471-2180
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
repourl:https://repositorio.unbosque.edu.co
url http://hdl.handle.net/20.500.12495/1571
https://doi.org/10.1186/s12866-019-1418-6
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv BMC Microbiology, 1471-2180, Vol.19, Num. 45, 2019 p.1-12
dc.relation.uri.none.fl_str_mv https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1418-6
dc.rights.*.fl_str_mv Attribution 4.0 International
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.local.spa.fl_str_mv Acceso abierto
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf69
dc.rights.creativecommons.none.fl_str_mv 2019
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
Acceso abierto
http://purl.org/coar/access_right/c_abf69
2019
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Biomed Central
dc.publisher.journal.spa.fl_str_mv BMC Microbiology
institution Universidad El Bosque
bitstream.url.fl_str_mv http://18.204.144.38/bitstreams/0cc7b9e3-1078-4b11-8a48-973d798c72d3/download
http://18.204.144.38/bitstreams/69e3770d-2022-4497-8db3-b5b3b62851b4/download
http://18.204.144.38/bitstreams/dd5836ec-e513-46cc-9892-42c684791e07/download
http://18.204.144.38/bitstreams/f300b825-de78-43e2-bc02-460e91bded27/download
http://18.204.144.38/bitstreams/8429a97c-0d3c-4776-b8a3-3d04eb48d10d/download
bitstream.checksum.fl_str_mv 1422d735975954ed4b8d26dc1803f7f4
0175ea4a2d4caec4bbcc37e300941108
8a4605be74aa9ea9d79846c1fba20a33
4262d8a37bd20039ccfa81a6eb248c97
25b4ca2b3b81f935038632e3c14b23c7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv DSpace Pre-instalado Biteca S.A.S
repository.mail.fl_str_mv bibliotecas@biteca.com
_version_ 1814100686964523008
spelling Castellanos, JaimeAbril Riaño, Deisy JuliethMoncayo-Ortiz, José IgnacioCorredor Rozo, Zayda LorenaReyes, NiradizTovar, CatalinaSánchez, Héctor FabioGuaca-González, Yina MarcelaLlanos-Uribe, Carmen ElisaOlarte, Narda MaríaVanegas, NatashaEscobar-Pérez, JavierCastro Cardozo, Betsy EsperanzaMarquez-Ortiz, Ricaurte AlejandroCastellanos, Jaime [0000-0003-1596-8383]Escobar-Pérez, Javier [0000-0002-0432-6978]Abril Riaño, Deisy Julieth [0000-0002-6686-6283]2019-07-29T15:01:24Z2019-07-29T15:01:24Z20191471-2180http://hdl.handle.net/20.500.12495/1571https://doi.org/10.1186/s12866-019-1418-6instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengBiomed CentralBMC MicrobiologyBMC Microbiology, 1471-2180, Vol.19, Num. 45, 2019 p.1-12https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1418-6Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Acceso abiertohttp://purl.org/coar/access_right/c_abf692019http://purl.org/coar/access_right/c_abf2Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clonearticleartículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501GenéticaBacterias aerobias gramnegativasInfecciones por pseudomonasCarbapenémicosbla KPC-2Pseudomonas aeruginosaCarbapenemsST235Background Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in blaKPC-2-positive P. aeruginosa ST235 in Colombia. Results In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the blaVIM and blaKPC-2 genes, respectively. The four blaKPC-2-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2″)-Ia, two Tn402-like with ant(3″)-Ia and blaOXA-2 and two Tn4401b with blaKPC-2. All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring blaKPC-2 were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background. Conclusion This is the first report of a double Tn4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism.ORIGINALAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdfAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdfapplication/pdf3663813http://18.204.144.38/bitstreams/0cc7b9e3-1078-4b11-8a48-973d798c72d3/download1422d735975954ed4b8d26dc1803f7f4MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8908http://18.204.144.38/bitstreams/69e3770d-2022-4497-8db3-b5b3b62851b4/download0175ea4a2d4caec4bbcc37e300941108MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://18.204.144.38/bitstreams/dd5836ec-e513-46cc-9892-42c684791e07/download8a4605be74aa9ea9d79846c1fba20a33MD53THUMBNAILAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdf.jpgAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdf.jpgIM Thumbnailimage/jpeg10349http://18.204.144.38/bitstreams/f300b825-de78-43e2-bc02-460e91bded27/download4262d8a37bd20039ccfa81a6eb248c97MD54TEXTAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdf.txtAbril D., Marquez-Ortiz R.A., Castro-Cardozo B., Moncayo-Ortiz J.I., Olarte Escobar N.M._2019.pdf.txtExtracted texttext/plain54131http://18.204.144.38/bitstreams/8429a97c-0d3c-4776-b8a3-3d04eb48d10d/download25b4ca2b3b81f935038632e3c14b23c7MD5520.500.12495/1571oai:18.204.144.38:20.500.12495/15712024-02-06 22:23:12.114http://creativecommons.org/licenses/by/4.0/Attribution 4.0 Internationalopen.accesshttp://18.204.144.38DSpace Pre-instalado Biteca S.A.Sbibliotecas@biteca.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