Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocysts

OocystsToxoplasma gondii and Cryptosporidium parvum were visualized using conventional optical microscopy (Olympus microscope CX31), allowing recognize their main morphological characteristics. In addition, its ability to autofluorescence when were exposed to certain range of ultraviolet light was c...

Full description

Autores:
Alba Patiño, Alejandra
Baker, Laura
Juez, Graciela
Ramírez, Andrés
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/5079
Acceso en línea:
http://hdl.handle.net/20.500.12495/5079
https://doi.org/10.1016/j.tmaid.2017.03.012
Palabra clave:
Toxoplasma gondii
Cryptosporidium parvum parasite
Autofluorescence
Microscopy
Rights
openAccess
License
Acceso abierto
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spelling Alba Patiño, AlejandraBaker, LauraJuez, GracielaRamírez, Andrés2020-11-18T16:29:27Z2020-11-18T16:29:27Z2017http://hdl.handle.net/20.500.12495/5079https://doi.org/10.1016/j.tmaid.2017.03.012instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengSpringer Link7th Latin American Congress on Biomedical Engineering, CLAIB 2016; Bucaramanga, Santander; Colombia; 26 October 2016 through 28 October 2016, IFMBE Proceedings, Vol. 60, 2017, Pages 281-284https://link.springer.com/chapter/10.1007/978-981-10-4086-3_71Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocystsOptical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocystsArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Toxoplasma gondiiCryptosporidium parvum parasiteAutofluorescenceMicroscopyOocystsToxoplasma gondii and Cryptosporidium parvum were visualized using conventional optical microscopy (Olympus microscope CX31), allowing recognize their main morphological characteristics. In addition, its ability to autofluorescence when were exposed to certain range of ultraviolet light was confirmed. Cryptosporidium parvum presented autofluorescence under the FITC filter (excitation 450-490 nm and emission 515 nm) and Toxoplasma gondii under (excitation 330-380 nm and emission 397 nm) UV filter. The above was done to develop an autofluorescence experimental protocol for recognition of C. parvum and T. gondii oocysts in samples of raw cow’s milk.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2017ORIGINALAlba_Alejandra_2017.pdfAlba_Alejandra_2017.pdfapplication/pdf656591https://repositorio.unbosque.edu.co/bitstreams/6a65c23e-e914-49b8-b0c1-79a5dec0349b/download107e4a9a92ed55aca7c330b93fac8419MD51THUMBNAILAlba_Alejandra_2017.pdf.jpgAlba_Alejandra_2017.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/e64e8f27-8141-4274-ac66-2db60261ae0f/download7210a811635d1799e7c05fee5d259be7MD52TEXTAlba_Alejandra_2017.pdf.txtAlba_Alejandra_2017.pdf.txtExtracted texttext/plain16173https://repositorio.unbosque.edu.co/bitstreams/8e80f2b9-2bd8-47e5-ab66-1147a503681b/download67fae1678b9a82efc7437bd979b62868MD5320.500.12495/5079oai:repositorio.unbosque.edu.co:20.500.12495/50792024-02-07 01:10:39.066restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.com
dc.title.spa.fl_str_mv Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocysts
dc.title.translated.spa.fl_str_mv Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocysts
title Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocysts
spellingShingle Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocysts
Toxoplasma gondii
Cryptosporidium parvum parasite
Autofluorescence
Microscopy
title_short Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocysts
title_full Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocysts
title_fullStr Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocysts
title_full_unstemmed Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocysts
title_sort Optical microscopy and autofluorescence recognition of toxoplasma gondii and cryptosporidium parvum oocysts
dc.creator.fl_str_mv Alba Patiño, Alejandra
Baker, Laura
Juez, Graciela
Ramírez, Andrés
dc.contributor.author.none.fl_str_mv Alba Patiño, Alejandra
Baker, Laura
Juez, Graciela
Ramírez, Andrés
dc.subject.keywords.spa.fl_str_mv Toxoplasma gondii
Cryptosporidium parvum parasite
Autofluorescence
Microscopy
topic Toxoplasma gondii
Cryptosporidium parvum parasite
Autofluorescence
Microscopy
description OocystsToxoplasma gondii and Cryptosporidium parvum were visualized using conventional optical microscopy (Olympus microscope CX31), allowing recognize their main morphological characteristics. In addition, its ability to autofluorescence when were exposed to certain range of ultraviolet light was confirmed. Cryptosporidium parvum presented autofluorescence under the FITC filter (excitation 450-490 nm and emission 515 nm) and Toxoplasma gondii under (excitation 330-380 nm and emission 397 nm) UV filter. The above was done to develop an autofluorescence experimental protocol for recognition of C. parvum and T. gondii oocysts in samples of raw cow’s milk.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2020-11-18T16:29:27Z
dc.date.available.none.fl_str_mv 2020-11-18T16:29:27Z
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dc.type.local.none.fl_str_mv Artículo de revista
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dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.tmaid.2017.03.012
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
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url http://hdl.handle.net/20.500.12495/5079
https://doi.org/10.1016/j.tmaid.2017.03.012
identifier_str_mv instname:Universidad El Bosque
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repourl:https://repositorio.unbosque.edu.co
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv 7th Latin American Congress on Biomedical Engineering, CLAIB 2016; Bucaramanga, Santander; Colombia; 26 October 2016 through 28 October 2016, IFMBE Proceedings, Vol. 60, 2017, Pages 281-284
dc.relation.uri.none.fl_str_mv https://link.springer.com/chapter/10.1007/978-981-10-4086-3_71
dc.rights.local.spa.fl_str_mv Acceso abierto
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
Acceso abierto
dc.rights.creativecommons.none.fl_str_mv 2017
rights_invalid_str_mv Acceso abierto
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2017
eu_rights_str_mv openAccess
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Springer Link
institution Universidad El Bosque
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