In vivo differential susceptibility of sensory neurons to rabies virus infection

There is controversy with regard to the entry pathway of the rabies virus (RABV) into the central nervous system (CNS). Some authors have suggested that the virus inoculated at the periphery is captured and transported to CNS only by motor neurons; however, it has been reported that dorsal root gang...

Full description

Autores:
Velandia-Romero, Myriam Lucía
Castellanos, Jaime
Martínez-Gutiérrez, Marlén
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/4597
Acceso en línea:
http://hdl.handle.net/20.500.12495/4597
https://doi.org/10.1007/s13365-013-0179-5
https://repositorio.unbosque.edu.co
Palabra clave:
Rabies virus
Sensory neurons
Motors neurons
Transynaptic infection
Rights
openAccess
License
Acceso abierto
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dc.title.spa.fl_str_mv In vivo differential susceptibility of sensory neurons to rabies virus infection
dc.title.translated.spa.fl_str_mv In vivo differential susceptibility of sensory neurons to rabies virus infection
title In vivo differential susceptibility of sensory neurons to rabies virus infection
spellingShingle In vivo differential susceptibility of sensory neurons to rabies virus infection
Rabies virus
Sensory neurons
Motors neurons
Transynaptic infection
title_short In vivo differential susceptibility of sensory neurons to rabies virus infection
title_full In vivo differential susceptibility of sensory neurons to rabies virus infection
title_fullStr In vivo differential susceptibility of sensory neurons to rabies virus infection
title_full_unstemmed In vivo differential susceptibility of sensory neurons to rabies virus infection
title_sort In vivo differential susceptibility of sensory neurons to rabies virus infection
dc.creator.fl_str_mv Velandia-Romero, Myriam Lucía
Castellanos, Jaime
Martínez-Gutiérrez, Marlén
dc.contributor.author.none.fl_str_mv Velandia-Romero, Myriam Lucía
Castellanos, Jaime
Martínez-Gutiérrez, Marlén
dc.contributor.orcid.none.fl_str_mv Castellanos, Jaime [0000-0003-1596-8383]
Velandia-Romero, Myriam Lucía [0000-0002-3340-7304]
dc.subject.keywords.spa.fl_str_mv Rabies virus
Sensory neurons
Motors neurons
Transynaptic infection
topic Rabies virus
Sensory neurons
Motors neurons
Transynaptic infection
description There is controversy with regard to the entry pathway of the rabies virus (RABV) into the central nervous system (CNS). Some authors have suggested that the virus inoculated at the periphery is captured and transported to CNS only by motor neurons; however, it has been reported that dorsal root ganglia (DRG) sensory neurons capture and transport the virus to the spinal cord (SC) and then to the brain. It is probable that preferences for one pathway or another depend on the site of inoculation and the post-infection time. Therefore, in the present study, we evaluated different vertebral segments and post-infection times, along with the location, number, and subpopulation of sensory neurons susceptible to infection after inoculating RABV in the footpads of adult mice. It was noted that the virus inoculated in the footpad preferentially entered the CNS through the large-sized DRG sensory neurons, while infection of the motor neurons occurred later. Further, it was found that the virus was dispersed in spinal cord trans-synaptically through the interneurons, arriving at both sensory neurons and contralateral motor neurons. In conclusion, we observed that RABV inoculated in the plantar footpad is captured preferentially by large sensory neurons and is transported to the DRG, where it replicates and is spread to the SC using transynaptic jumps, infecting sensory and motor neurons at the same level before ascending to the brain.
publishDate 2013
dc.date.issued.none.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2020-10-28T22:00:19Z
dc.date.available.none.fl_str_mv 2020-10-28T22:00:19Z
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dc.type.local.none.fl_str_mv Artículo de revista
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dc.identifier.issn.none.fl_str_mv 1355-0284
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dc.identifier.doi.none.fl_str_mv https://doi.org/10.1007/s13365-013-0179-5
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
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identifier_str_mv 1355-0284
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url http://hdl.handle.net/20.500.12495/4597
https://doi.org/10.1007/s13365-013-0179-5
https://repositorio.unbosque.edu.co
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Journal of NeuroVirology, 1355-0284, Vol. 19, 2013, p. 367–375
dc.relation.uri.none.fl_str_mv https://link-springer-com.ezproxy.unbosque.edu.co/article/10.1007/s13365-013-0179-5
dc.rights.local.spa.fl_str_mv Acceso abierto
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dc.rights.creativecommons.none.fl_str_mv 2013-08-20
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2013-08-20
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dc.publisher.spa.fl_str_mv Springer
dc.publisher.journal.spa.fl_str_mv Journal of NeuroVirology
institution Universidad El Bosque
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spelling Velandia-Romero, Myriam LucíaCastellanos, JaimeMartínez-Gutiérrez, MarlénCastellanos, Jaime [0000-0003-1596-8383]Velandia-Romero, Myriam Lucía [0000-0002-3340-7304]2020-10-28T22:00:19Z2020-10-28T22:00:19Z20131355-0284http://hdl.handle.net/20.500.12495/4597https://doi.org/10.1007/s13365-013-0179-5instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengSpringerJournal of NeuroVirologyJournal of NeuroVirology, 1355-0284, Vol. 19, 2013, p. 367–375https://link-springer-com.ezproxy.unbosque.edu.co/article/10.1007/s13365-013-0179-5In vivo differential susceptibility of sensory neurons to rabies virus infectionIn vivo differential susceptibility of sensory neurons to rabies virus infectionArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Rabies virusSensory neuronsMotors neuronsTransynaptic infectionThere is controversy with regard to the entry pathway of the rabies virus (RABV) into the central nervous system (CNS). Some authors have suggested that the virus inoculated at the periphery is captured and transported to CNS only by motor neurons; however, it has been reported that dorsal root ganglia (DRG) sensory neurons capture and transport the virus to the spinal cord (SC) and then to the brain. It is probable that preferences for one pathway or another depend on the site of inoculation and the post-infection time. Therefore, in the present study, we evaluated different vertebral segments and post-infection times, along with the location, number, and subpopulation of sensory neurons susceptible to infection after inoculating RABV in the footpads of adult mice. It was noted that the virus inoculated in the footpad preferentially entered the CNS through the large-sized DRG sensory neurons, while infection of the motor neurons occurred later. Further, it was found that the virus was dispersed in spinal cord trans-synaptically through the interneurons, arriving at both sensory neurons and contralateral motor neurons. In conclusion, we observed that RABV inoculated in the plantar footpad is captured preferentially by large sensory neurons and is transported to the DRG, where it replicates and is spread to the SC using transynaptic jumps, infecting sensory and motor neurons at the same level before ascending to the brain.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2013-08-20ORIGINALVelandia-Romero, Myriam L..pdfVelandia-Romero, Myriam L..pdfapplication/pdf676131https://repositorio.unbosque.edu.co/bitstreams/67b01bf1-6ca4-4582-8573-16e7971e466c/download8f75bfe095d06c032fae2d78abf9a32fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/dc47c5d6-db01-4458-ba62-c05192e0e994/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILVelandia-Romero, Myriam L..pdf.jpgVelandia-Romero, Myriam L..pdf.jpgIM Thumbnailimage/jpeg9068https://repositorio.unbosque.edu.co/bitstreams/cd7dd82e-77fa-45ad-a32f-8bdadcf863b7/download170464823eb010ec32e40f3144187ef1MD53TEXTVelandia-Romero, Myriam L..pdf.txtVelandia-Romero, Myriam L..pdf.txtExtracted texttext/plain37624https://repositorio.unbosque.edu.co/bitstreams/a77fbb1b-e12b-4c7f-85b5-35f1b84fae82/download3ed3401f025b08469e442d4e46f652f5MD5420.500.12495/4597oai:repositorio.unbosque.edu.co:20.500.12495/45972024-02-07 04:29:05.946open.accesshttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.comTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=