Rapid identification of Enterococcus hirae and Enterococcus durans by PCR and detection of a homologue of the E. hirae mur-2 gene in E. durans

During an evaluation of PCR for identification of isolates of Enterococcus hirae, a homologue with 82% identity to E. hirae mur-2 was identified in Enterococcus durans and was named mur-2ed. PCR using primers for two genes (copY and murG) of E. hirae strains showed amplification with E. hirae strains only. PCR (under high-stringency conditions) with primers for the mur-2ed gene gave the expected amplification product only with E. durans strains. A combination of murG and mur-2ed primers in a multiplex PCR assay differentiated E. hirae from E. durans in all cases. PCR using these primers appears to be a rapid alternative for identification of E. hirae and E. durans isolates.

• Autores: Arias, César A.; Robredo Valgañón, Beatriz; Singh, Kavindra V.; Torres, Carmen; panesso, diana; Beral, Valerie
• Tipo de recurso: Article of journal
• Fecha de publicación: 2006
• Institución: Universidad El Bosque
• Repositorio: Repositorio U. El Bosque
• Idioma: eng
• OAI Identifier: oai:repositorio.unbosque.edu.co:20.500.12495/5379
• Acceso en línea: http://hdl.handle.net/20.500.12495/5379; https://dx.doi.org/10.1128%2FJCM.44.4.1567-1570.2006; https://repositorio.unbosque.edu.co
• Palabra clave: Enterococcus; Genoma bacteriano; Reacción en cadena de la polimerasa
• Rights: openAccess
• License: Acceso abierto