Rapid identification of Enterococcus hirae and Enterococcus durans by PCR and detection of a homologue of the E. hirae mur-2 gene in E. durans
During an evaluation of PCR for identification of isolates of Enterococcus hirae, a homologue with 82% identity to E. hirae mur-2 was identified in Enterococcus durans and was named mur-2ed. PCR using primers for two genes (copY and murG) of E. hirae strains showed amplification with E. hirae strain...
- Autores:
-
Arias, César A.
Robredo Valgañón, Beatriz
Singh, Kavindra V.
Torres, Carmen
panesso, diana
Beral, Valerie
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2006
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/5379
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/5379
https://dx.doi.org/10.1128%2FJCM.44.4.1567-1570.2006
https://repositorio.unbosque.edu.co
- Palabra clave:
- Enterococcus
Genoma bacteriano
Reacción en cadena de la polimerasa
- Rights
- openAccess
- License
- Acceso abierto
| Summary: | During an evaluation of PCR for identification of isolates of Enterococcus hirae, a homologue with 82% identity to E. hirae mur-2 was identified in Enterococcus durans and was named mur-2ed. PCR using primers for two genes (copY and murG) of E. hirae strains showed amplification with E. hirae strains only. PCR (under high-stringency conditions) with primers for the mur-2ed gene gave the expected amplification product only with E. durans strains. A combination of murG and mur-2ed primers in a multiplex PCR assay differentiated E. hirae from E. durans in all cases. PCR using these primers appears to be a rapid alternative for identification of E. hirae and E. durans isolates. |
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