A low-cost electrochemical biosensor platform for C-reactive protein detection
Antibiotic resistance is a worldwide problem aggravated by the overuse of prophylactic antibiotic therapies and lack of real-time biosensing equipment to differentiate viral from bacterial infections at the Point of Care (POC), particularly in rural areas with limited access to clinical laboratory f...
- Autores:
-
Guillem, Pedro
Bustos, Rosa Helena
Garzon, Vivian
Munoz, Andres
Juez, Graciela
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/5568
- Palabra clave:
- Electrochemical biosensor
Antibiotic resistance
CRP
Point-of-care diagnostics
- Rights
- openAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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Repositorio U. El Bosque |
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|
dc.title.spa.fl_str_mv |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
dc.title.translated.spa.fl_str_mv |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
title |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
spellingShingle |
A low-cost electrochemical biosensor platform for C-reactive protein detection Electrochemical biosensor Antibiotic resistance CRP Point-of-care diagnostics |
title_short |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
title_full |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
title_fullStr |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
title_full_unstemmed |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
title_sort |
A low-cost electrochemical biosensor platform for C-reactive protein detection |
dc.creator.fl_str_mv |
Guillem, Pedro Bustos, Rosa Helena Garzon, Vivian Munoz, Andres Juez, Graciela |
dc.contributor.author.none.fl_str_mv |
Guillem, Pedro Bustos, Rosa Helena Garzon, Vivian Munoz, Andres Juez, Graciela |
dc.subject.keywords.spa.fl_str_mv |
Electrochemical biosensor Antibiotic resistance CRP Point-of-care diagnostics |
topic |
Electrochemical biosensor Antibiotic resistance CRP Point-of-care diagnostics |
description |
Antibiotic resistance is a worldwide problem aggravated by the overuse of prophylactic antibiotic therapies and lack of real-time biosensing equipment to differentiate viral from bacterial infections at the Point of Care (POC), particularly in rural areas with limited access to clinical laboratory facilities. As recent studies reveal the potential of C-Reactive Protein (CRP) and other acute phase biomarkers to achieve early determination of etiology in acute febrile illness, novel biosensing equipment become a plausible approach in preventing the unnecessary prescription of antibiotics. A low-cost experimental platform was engineered to measure CRP concentrations in 50 μL samples of buffer and enriched plasma, using label-free antigenic probes, legacy electrochemical methods and open-source hardware. The prototype presents a portable, cost-effective device for use at the POC with a simplified user interface that can be used wirelessly by healthcare professionals using a mobile phone or laptop. Based on the measured CRP levels, the device suggests if the acute febrile episode is likely to be of bacterial etiology. |
publishDate |
2021 |
dc.date.accessioned.none.fl_str_mv |
2021-03-08T16:35:20Z |
dc.date.available.none.fl_str_mv |
2021-03-08T16:35:20Z |
dc.date.issued.none.fl_str_mv |
2021 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.none.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.issn.none.fl_str_mv |
2214-1804 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/5568 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.sbsr.2021.100402 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
repourl:https://repositorio.unbosque.edu.co |
identifier_str_mv |
2214-1804 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque repourl:https://repositorio.unbosque.edu.co |
url |
http://hdl.handle.net/20.500.12495/5568 https://doi.org/10.1016/j.sbsr.2021.100402 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Sensing and Bio-Sensing Research, 2214-1804, Vol. 31, 2021 p. 100402 |
dc.relation.uri.none.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S2214180421000076?via%3Dihub |
dc.rights.*.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.rights.local.spa.fl_str_mv |
Acceso abierto |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Acceso abierto |
dc.rights.creativecommons.none.fl_str_mv |
2021 |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ Acceso abierto http://purl.org/coar/access_right/c_abf2 2021 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Elsevier B.V. |
dc.publisher.journal.spa.fl_str_mv |
Sensing and Bio-Sensing Research |
institution |
Universidad El Bosque |
bitstream.url.fl_str_mv |
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Repositorio Institucional Universidad El Bosque |
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Guillem, PedroBustos, Rosa HelenaGarzon, VivianMunoz, AndresJuez, Graciela2021-03-08T16:35:20Z2021-03-08T16:35:20Z20212214-1804http://hdl.handle.net/20.500.12495/5568https://doi.org/10.1016/j.sbsr.2021.100402instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengElsevier B.V.Sensing and Bio-Sensing ResearchSensing and Bio-Sensing Research, 2214-1804, Vol. 31, 2021 p. 100402https://www.sciencedirect.com/science/article/pii/S2214180421000076?via%3DihubAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2021A low-cost electrochemical biosensor platform for C-reactive protein detectionA low-cost electrochemical biosensor platform for C-reactive protein detectionArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Electrochemical biosensorAntibiotic resistanceCRPPoint-of-care diagnosticsAntibiotic resistance is a worldwide problem aggravated by the overuse of prophylactic antibiotic therapies and lack of real-time biosensing equipment to differentiate viral from bacterial infections at the Point of Care (POC), particularly in rural areas with limited access to clinical laboratory facilities. As recent studies reveal the potential of C-Reactive Protein (CRP) and other acute phase biomarkers to achieve early determination of etiology in acute febrile illness, novel biosensing equipment become a plausible approach in preventing the unnecessary prescription of antibiotics. A low-cost experimental platform was engineered to measure CRP concentrations in 50 μL samples of buffer and enriched plasma, using label-free antigenic probes, legacy electrochemical methods and open-source hardware. The prototype presents a portable, cost-effective device for use at the POC with a simplified user interface that can be used wirelessly by healthcare professionals using a mobile phone or laptop. Based on the measured CRP levels, the device suggests if the acute febrile episode is likely to be of bacterial etiology.ORIGINALGuillem_Pedro_2021.pdfGuillem_Pedro_2021.pdfapplication/pdf2490192https://repositorio.unbosque.edu.co/bitstreams/17cae090-389e-4017-bab9-480b7cea0c2a/download0486a59f0ffedb7a518745c1d81d1bc5MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805https://repositorio.unbosque.edu.co/bitstreams/2a5014aa-6c07-454a-930e-0e39e50fc563/download4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/8d910464-786f-4236-aedd-4ff0eba87560/download8a4605be74aa9ea9d79846c1fba20a33MD53THUMBNAILGuillem_Pedro_2021.pdf.jpgGuillem_Pedro_2021.pdf.jpgIM Thumbnailimage/jpeg10300https://repositorio.unbosque.edu.co/bitstreams/ba6232eb-ae27-412a-8488-fb6f1bf3c555/downloaddd062a7f88f12d861ec5b4946882ddeeMD54TEXTGuillem_Pedro_2021.pdf.txtGuillem_Pedro_2021.pdf.txtExtracted texttext/plain63448https://repositorio.unbosque.edu.co/bitstreams/faa7eeab-70a2-43ff-9870-414f80721e9e/download03de9449b82189e8a1836092e185a2fbMD5520.500.12495/5568oai:repositorio.unbosque.edu.co:20.500.12495/55682024-02-07 10:08:03.707http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internacionalopen.accesshttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.comTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |