A low-cost electrochemical biosensor platform for C-reactive protein detection

Antibiotic resistance is a worldwide problem aggravated by the overuse of prophylactic antibiotic therapies and lack of real-time biosensing equipment to differentiate viral from bacterial infections at the Point of Care (POC), particularly in rural areas with limited access to clinical laboratory f...

Full description

Autores:
Guillem, Pedro
Bustos, Rosa Helena
Garzon, Vivian
Munoz, Andres
Juez, Graciela
Tipo de recurso:
Article of journal
Fecha de publicación:
2021
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/5568
Acceso en línea:
http://hdl.handle.net/20.500.12495/5568
https://doi.org/10.1016/j.sbsr.2021.100402
Palabra clave:
Electrochemical biosensor
Antibiotic resistance
CRP
Point-of-care diagnostics
Rights
openAccess
License
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Description
Summary:Antibiotic resistance is a worldwide problem aggravated by the overuse of prophylactic antibiotic therapies and lack of real-time biosensing equipment to differentiate viral from bacterial infections at the Point of Care (POC), particularly in rural areas with limited access to clinical laboratory facilities. As recent studies reveal the potential of C-Reactive Protein (CRP) and other acute phase biomarkers to achieve early determination of etiology in acute febrile illness, novel biosensing equipment become a plausible approach in preventing the unnecessary prescription of antibiotics. A low-cost experimental platform was engineered to measure CRP concentrations in 50 μL samples of buffer and enriched plasma, using label-free antigenic probes, legacy electrochemical methods and open-source hardware. The prototype presents a portable, cost-effective device for use at the POC with a simplified user interface that can be used wirelessly by healthcare professionals using a mobile phone or laptop. Based on the measured CRP levels, the device suggests if the acute febrile episode is likely to be of bacterial etiology.