Comprehensive genomic profiling of combined small cell lung cancer
Background: Combined small cell lung cancer (CSCLC) is an uncommon and heterogeneous subtype of small cell lung cancer (SCLC). However, there is limited data concerning the different molecular changes and clinical features in CSCLC compared to pure SCLC. Methods: The clinical and pathological charac...
- Autores:
-
Zhang, Jing
Zhang, Liping
Luo, Jie
Ge, Tao
Fan, Pengyu
Sun, Liangdong
Hou, Likun
Li, Junqiang
Yu, Huansha
Wu, Chunxiao
Zhu, Yuming
Wu, Chunyan
Jiang, Gening
Troncone, Giancarlo
Malhotra, Jyoti
Okuda, Katsuhiro
Santarpia, Mariacarmela
Zamarchi, Rita
Goto, Taichiro
Cardona, Andrés F.
Xu, Jianfang
Chen, Qiankun
Zhang, Zhonghong
Zhang, Peng
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/8686
- Palabra clave:
- Cáncer de pulmón de células pequeñas combinado (CPCP)
Cáncer de pulmón de células pequeñas (CPCP)
Perfiles genómicos completos
Secuenciación genética dirigida
Combined small cell lung cancer (CSCLC)
Small cell lung cancer (SCLC)
Comprehensive genomic profiling
Targeted gene sequencing
- Rights
- closedAccess
- License
- Acceso cerrado
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Repositorio U. El Bosque |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Comprehensive genomic profiling of combined small cell lung cancer |
dc.title.translated.spa.fl_str_mv |
Comprehensive genomic profiling of combined small cell lung cancer |
title |
Comprehensive genomic profiling of combined small cell lung cancer |
spellingShingle |
Comprehensive genomic profiling of combined small cell lung cancer Cáncer de pulmón de células pequeñas combinado (CPCP) Cáncer de pulmón de células pequeñas (CPCP) Perfiles genómicos completos Secuenciación genética dirigida Combined small cell lung cancer (CSCLC) Small cell lung cancer (SCLC) Comprehensive genomic profiling Targeted gene sequencing |
title_short |
Comprehensive genomic profiling of combined small cell lung cancer |
title_full |
Comprehensive genomic profiling of combined small cell lung cancer |
title_fullStr |
Comprehensive genomic profiling of combined small cell lung cancer |
title_full_unstemmed |
Comprehensive genomic profiling of combined small cell lung cancer |
title_sort |
Comprehensive genomic profiling of combined small cell lung cancer |
dc.creator.fl_str_mv |
Zhang, Jing Zhang, Liping Luo, Jie Ge, Tao Fan, Pengyu Sun, Liangdong Hou, Likun Li, Junqiang Yu, Huansha Wu, Chunxiao Zhu, Yuming Wu, Chunyan Jiang, Gening Troncone, Giancarlo Malhotra, Jyoti Okuda, Katsuhiro Santarpia, Mariacarmela Zamarchi, Rita Goto, Taichiro Cardona, Andrés F. Xu, Jianfang Chen, Qiankun Zhang, Zhonghong Zhang, Peng |
dc.contributor.author.none.fl_str_mv |
Zhang, Jing Zhang, Liping Luo, Jie Ge, Tao Fan, Pengyu Sun, Liangdong Hou, Likun Li, Junqiang Yu, Huansha Wu, Chunxiao Zhu, Yuming Wu, Chunyan Jiang, Gening Troncone, Giancarlo Malhotra, Jyoti Okuda, Katsuhiro Santarpia, Mariacarmela Zamarchi, Rita Goto, Taichiro Cardona, Andrés F. Xu, Jianfang Chen, Qiankun Zhang, Zhonghong Zhang, Peng |
dc.subject.spa.fl_str_mv |
Cáncer de pulmón de células pequeñas combinado (CPCP) Cáncer de pulmón de células pequeñas (CPCP) Perfiles genómicos completos Secuenciación genética dirigida |
topic |
Cáncer de pulmón de células pequeñas combinado (CPCP) Cáncer de pulmón de células pequeñas (CPCP) Perfiles genómicos completos Secuenciación genética dirigida Combined small cell lung cancer (CSCLC) Small cell lung cancer (SCLC) Comprehensive genomic profiling Targeted gene sequencing |
dc.subject.keywords.spa.fl_str_mv |
Combined small cell lung cancer (CSCLC) Small cell lung cancer (SCLC) Comprehensive genomic profiling Targeted gene sequencing |
description |
Background: Combined small cell lung cancer (CSCLC) is an uncommon and heterogeneous subtype of small cell lung cancer (SCLC). However, there is limited data concerning the different molecular changes and clinical features in CSCLC compared to pure SCLC. Methods: The clinical and pathological characteristics of pure SCLC and CSCLC patients were analyzed. Immunohistochemistry and microdissection were performed to isolate the CSCLC components. Further molecular analysis was carried out by next-generation sequencing (NGS) in 12 CSCLC and 30 pure SCLC. Results: There were no significant differences in clinical features between CSCLC and pure SCLC. Overall survival (OS) of CSCLC patients was worse than pure SCLC (P=0.005). NGS results indicated that TP53 and RB1 were the most frequently mutated genes in both CSCLC (83.33% and 66.67%) and pure SCLC (80.00% and 63.33%) groups. However, less than 10% common mutations were found in both CSCLC and pure SCLC. When analyzing the data of SCLC and non-small cell lung cancer (NSCLC) components of CSCLC, more than 50% common mutations, and identical genes with mutations were detected. Moreover, there were also common biological processes and signaling pathways identified in CSCLC and pure SCLC, in addition to SCLC and NSCLC components. Conclusions: There were no significant differences in terms of clinical features between CSCLC and pure SCLC. However, the prognosis for CSCLC was worse than pure SCLC. NGS analysis suggested that CSCLC components might derive from the same pluripotent single clone with common initial molecular alterations and subsequent acquisitions of other genetic mutations. |
publishDate |
2021 |
dc.date.issued.none.fl_str_mv |
2021 |
dc.date.accessioned.none.fl_str_mv |
2022-08-08T23:33:35Z |
dc.date.available.none.fl_str_mv |
2022-08-08T23:33:35Z |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.none.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.issn.none.fl_str_mv |
2226-4477 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/8686 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.21037/TLCR-20-1099 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
repourl:https://repositorio.unbosque.edu.co |
identifier_str_mv |
2226-4477 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque repourl:https://repositorio.unbosque.edu.co |
url |
http://hdl.handle.net/20.500.12495/8686 https://doi.org/10.21037/TLCR-20-1099 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Translational Lung Cancer Research, 2226-4477, Vol 10, No 2, February, 2021 |
dc.relation.uri.none.fl_str_mv |
https://tlcr.amegroups.com/article/view/48244/html |
dc.rights.local.spa.fl_str_mv |
Acceso cerrado |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_14cb info:eu-repo/semantics/closedAccess |
rights_invalid_str_mv |
Acceso cerrado http://purl.org/coar/access_right/c_14cb |
eu_rights_str_mv |
closedAccess |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
AME Publishing Company |
dc.publisher.journal.spa.fl_str_mv |
Translational Lung Cancer Research |
institution |
Universidad El Bosque |
bitstream.url.fl_str_mv |
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spelling |
Zhang, JingZhang, LipingLuo, JieGe, TaoFan, PengyuSun, LiangdongHou, LikunLi, JunqiangYu, HuanshaWu, ChunxiaoZhu, YumingWu, ChunyanJiang, GeningTroncone, GiancarloMalhotra, JyotiOkuda, KatsuhiroSantarpia, MariacarmelaZamarchi, RitaGoto, TaichiroCardona, Andrés F.Xu, JianfangChen, QiankunZhang, ZhonghongZhang, Peng2022-08-08T23:33:35Z2022-08-08T23:33:35Z20212226-4477http://hdl.handle.net/20.500.12495/8686https://doi.org/10.21037/TLCR-20-1099instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengAME Publishing CompanyTranslational Lung Cancer ResearchTranslational Lung Cancer Research, 2226-4477, Vol 10, No 2, February, 2021https://tlcr.amegroups.com/article/view/48244/htmlCáncer de pulmón de células pequeñas combinado (CPCP)Cáncer de pulmón de células pequeñas (CPCP)Perfiles genómicos completosSecuenciación genética dirigidaCombined small cell lung cancer (CSCLC)Small cell lung cancer (SCLC)Comprehensive genomic profilingTargeted gene sequencingComprehensive genomic profiling of combined small cell lung cancerComprehensive genomic profiling of combined small cell lung cancerArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Background: Combined small cell lung cancer (CSCLC) is an uncommon and heterogeneous subtype of small cell lung cancer (SCLC). However, there is limited data concerning the different molecular changes and clinical features in CSCLC compared to pure SCLC. Methods: The clinical and pathological characteristics of pure SCLC and CSCLC patients were analyzed. Immunohistochemistry and microdissection were performed to isolate the CSCLC components. Further molecular analysis was carried out by next-generation sequencing (NGS) in 12 CSCLC and 30 pure SCLC. Results: There were no significant differences in clinical features between CSCLC and pure SCLC. Overall survival (OS) of CSCLC patients was worse than pure SCLC (P=0.005). NGS results indicated that TP53 and RB1 were the most frequently mutated genes in both CSCLC (83.33% and 66.67%) and pure SCLC (80.00% and 63.33%) groups. However, less than 10% common mutations were found in both CSCLC and pure SCLC. When analyzing the data of SCLC and non-small cell lung cancer (NSCLC) components of CSCLC, more than 50% common mutations, and identical genes with mutations were detected. Moreover, there were also common biological processes and signaling pathways identified in CSCLC and pure SCLC, in addition to SCLC and NSCLC components. Conclusions: There were no significant differences in terms of clinical features between CSCLC and pure SCLC. However, the prognosis for CSCLC was worse than pure SCLC. NGS analysis suggested that CSCLC components might derive from the same pluripotent single clone with common initial molecular alterations and subsequent acquisitions of other genetic mutations.Acceso cerradohttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessORIGINALZhang_Jing_2021.pdfZhang_Jing_2021.pdfapplication/pdf1951716https://repositorio.unbosque.edu.co/bitstreams/8b05b633-6b07-42d1-a8c5-107b88896df4/download011d1a09c8608ec9745f148c97dd49a8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/2eee71a7-6918-4ac5-a2e7-bd9a1d4d6843/download8a4605be74aa9ea9d79846c1fba20a33MD53THUMBNAILZhang_Jing_2021.pdf.jpgZhang_Jing_2021.pdf.jpgIM Thumbnailimage/jpeg10303https://repositorio.unbosque.edu.co/bitstreams/811d3be8-1a58-4c38-9bae-9036125c150a/downloadd3c14bd7d2f534bfd20b016069c57892MD54TEXTZhang_Jing_2021.pdf.txtZhang_Jing_2021.pdf.txtExtracted texttext/plain70968https://repositorio.unbosque.edu.co/bitstreams/41f801d2-614d-41f6-82ae-45790d545fe2/download850077458456987521e80311ff44f271MD5520.500.12495/8686oai:repositorio.unbosque.edu.co:20.500.12495/86862024-02-06 23:45:39.419restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |