Comprehensive genomic profiling of combined small cell lung cancer

Background: Combined small cell lung cancer (CSCLC) is an uncommon and heterogeneous subtype of small cell lung cancer (SCLC). However, there is limited data concerning the different molecular changes and clinical features in CSCLC compared to pure SCLC. Methods: The clinical and pathological charac...

Full description

Autores:
Zhang, Jing
Zhang, Liping
Luo, Jie
Ge, Tao
Fan, Pengyu
Sun, Liangdong
Hou, Likun
Li, Junqiang
Yu, Huansha
Wu, Chunxiao
Zhu, Yuming
Wu, Chunyan
Jiang, Gening
Troncone, Giancarlo
Malhotra, Jyoti
Okuda, Katsuhiro
Santarpia, Mariacarmela
Zamarchi, Rita
Goto, Taichiro
Cardona, Andrés F.
Xu, Jianfang
Chen, Qiankun
Zhang, Zhonghong
Zhang, Peng
Tipo de recurso:
Article of journal
Fecha de publicación:
2021
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/8686
Acceso en línea:
http://hdl.handle.net/20.500.12495/8686
https://doi.org/10.21037/TLCR-20-1099
Palabra clave:
Cáncer de pulmón de células pequeñas combinado (CPCP)
Cáncer de pulmón de células pequeñas (CPCP)
Perfiles genómicos completos
Secuenciación genética dirigida
Combined small cell lung cancer (CSCLC)
Small cell lung cancer (SCLC)
Comprehensive genomic profiling
Targeted gene sequencing
Rights
closedAccess
License
Acceso cerrado
id UNBOSQUE2_9f60be9b17c93bb542eb591f191c8230
oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/8686
network_acronym_str UNBOSQUE2
network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.spa.fl_str_mv Comprehensive genomic profiling of combined small cell lung cancer
dc.title.translated.spa.fl_str_mv Comprehensive genomic profiling of combined small cell lung cancer
title Comprehensive genomic profiling of combined small cell lung cancer
spellingShingle Comprehensive genomic profiling of combined small cell lung cancer
Cáncer de pulmón de células pequeñas combinado (CPCP)
Cáncer de pulmón de células pequeñas (CPCP)
Perfiles genómicos completos
Secuenciación genética dirigida
Combined small cell lung cancer (CSCLC)
Small cell lung cancer (SCLC)
Comprehensive genomic profiling
Targeted gene sequencing
title_short Comprehensive genomic profiling of combined small cell lung cancer
title_full Comprehensive genomic profiling of combined small cell lung cancer
title_fullStr Comprehensive genomic profiling of combined small cell lung cancer
title_full_unstemmed Comprehensive genomic profiling of combined small cell lung cancer
title_sort Comprehensive genomic profiling of combined small cell lung cancer
dc.creator.fl_str_mv Zhang, Jing
Zhang, Liping
Luo, Jie
Ge, Tao
Fan, Pengyu
Sun, Liangdong
Hou, Likun
Li, Junqiang
Yu, Huansha
Wu, Chunxiao
Zhu, Yuming
Wu, Chunyan
Jiang, Gening
Troncone, Giancarlo
Malhotra, Jyoti
Okuda, Katsuhiro
Santarpia, Mariacarmela
Zamarchi, Rita
Goto, Taichiro
Cardona, Andrés F.
Xu, Jianfang
Chen, Qiankun
Zhang, Zhonghong
Zhang, Peng
dc.contributor.author.none.fl_str_mv Zhang, Jing
Zhang, Liping
Luo, Jie
Ge, Tao
Fan, Pengyu
Sun, Liangdong
Hou, Likun
Li, Junqiang
Yu, Huansha
Wu, Chunxiao
Zhu, Yuming
Wu, Chunyan
Jiang, Gening
Troncone, Giancarlo
Malhotra, Jyoti
Okuda, Katsuhiro
Santarpia, Mariacarmela
Zamarchi, Rita
Goto, Taichiro
Cardona, Andrés F.
Xu, Jianfang
Chen, Qiankun
Zhang, Zhonghong
Zhang, Peng
dc.subject.spa.fl_str_mv Cáncer de pulmón de células pequeñas combinado (CPCP)
Cáncer de pulmón de células pequeñas (CPCP)
Perfiles genómicos completos
Secuenciación genética dirigida
topic Cáncer de pulmón de células pequeñas combinado (CPCP)
Cáncer de pulmón de células pequeñas (CPCP)
Perfiles genómicos completos
Secuenciación genética dirigida
Combined small cell lung cancer (CSCLC)
Small cell lung cancer (SCLC)
Comprehensive genomic profiling
Targeted gene sequencing
dc.subject.keywords.spa.fl_str_mv Combined small cell lung cancer (CSCLC)
Small cell lung cancer (SCLC)
Comprehensive genomic profiling
Targeted gene sequencing
description Background: Combined small cell lung cancer (CSCLC) is an uncommon and heterogeneous subtype of small cell lung cancer (SCLC). However, there is limited data concerning the different molecular changes and clinical features in CSCLC compared to pure SCLC. Methods: The clinical and pathological characteristics of pure SCLC and CSCLC patients were analyzed. Immunohistochemistry and microdissection were performed to isolate the CSCLC components. Further molecular analysis was carried out by next-generation sequencing (NGS) in 12 CSCLC and 30 pure SCLC. Results: There were no significant differences in clinical features between CSCLC and pure SCLC. Overall survival (OS) of CSCLC patients was worse than pure SCLC (P=0.005). NGS results indicated that TP53 and RB1 were the most frequently mutated genes in both CSCLC (83.33% and 66.67%) and pure SCLC (80.00% and 63.33%) groups. However, less than 10% common mutations were found in both CSCLC and pure SCLC. When analyzing the data of SCLC and non-small cell lung cancer (NSCLC) components of CSCLC, more than 50% common mutations, and identical genes with mutations were detected. Moreover, there were also common biological processes and signaling pathways identified in CSCLC and pure SCLC, in addition to SCLC and NSCLC components. Conclusions: There were no significant differences in terms of clinical features between CSCLC and pure SCLC. However, the prognosis for CSCLC was worse than pure SCLC. NGS analysis suggested that CSCLC components might derive from the same pluripotent single clone with common initial molecular alterations and subsequent acquisitions of other genetic mutations.
publishDate 2021
dc.date.issued.none.fl_str_mv 2021
dc.date.accessioned.none.fl_str_mv 2022-08-08T23:33:35Z
dc.date.available.none.fl_str_mv 2022-08-08T23:33:35Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.none.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
format http://purl.org/coar/resource_type/c_6501
dc.identifier.issn.none.fl_str_mv 2226-4477
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/8686
dc.identifier.doi.none.fl_str_mv https://doi.org/10.21037/TLCR-20-1099
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv repourl:https://repositorio.unbosque.edu.co
identifier_str_mv 2226-4477
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
repourl:https://repositorio.unbosque.edu.co
url http://hdl.handle.net/20.500.12495/8686
https://doi.org/10.21037/TLCR-20-1099
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Translational Lung Cancer Research, 2226-4477, Vol 10, No 2, February, 2021
dc.relation.uri.none.fl_str_mv https://tlcr.amegroups.com/article/view/48244/html
dc.rights.local.spa.fl_str_mv Acceso cerrado
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
info:eu-repo/semantics/closedAccess
rights_invalid_str_mv Acceso cerrado
http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv closedAccess
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv AME Publishing Company
dc.publisher.journal.spa.fl_str_mv Translational Lung Cancer Research
institution Universidad El Bosque
bitstream.url.fl_str_mv https://repositorio.unbosque.edu.co/bitstreams/8b05b633-6b07-42d1-a8c5-107b88896df4/download
https://repositorio.unbosque.edu.co/bitstreams/2eee71a7-6918-4ac5-a2e7-bd9a1d4d6843/download
https://repositorio.unbosque.edu.co/bitstreams/811d3be8-1a58-4c38-9bae-9036125c150a/download
https://repositorio.unbosque.edu.co/bitstreams/41f801d2-614d-41f6-82ae-45790d545fe2/download
bitstream.checksum.fl_str_mv 011d1a09c8608ec9745f148c97dd49a8
8a4605be74aa9ea9d79846c1fba20a33
d3c14bd7d2f534bfd20b016069c57892
850077458456987521e80311ff44f271
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad El Bosque
repository.mail.fl_str_mv bibliotecas@biteca.com
_version_ 1814100715497324544
spelling Zhang, JingZhang, LipingLuo, JieGe, TaoFan, PengyuSun, LiangdongHou, LikunLi, JunqiangYu, HuanshaWu, ChunxiaoZhu, YumingWu, ChunyanJiang, GeningTroncone, GiancarloMalhotra, JyotiOkuda, KatsuhiroSantarpia, MariacarmelaZamarchi, RitaGoto, TaichiroCardona, Andrés F.Xu, JianfangChen, QiankunZhang, ZhonghongZhang, Peng2022-08-08T23:33:35Z2022-08-08T23:33:35Z20212226-4477http://hdl.handle.net/20.500.12495/8686https://doi.org/10.21037/TLCR-20-1099instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengAME Publishing CompanyTranslational Lung Cancer ResearchTranslational Lung Cancer Research, 2226-4477, Vol 10, No 2, February, 2021https://tlcr.amegroups.com/article/view/48244/htmlCáncer de pulmón de células pequeñas combinado (CPCP)Cáncer de pulmón de células pequeñas (CPCP)Perfiles genómicos completosSecuenciación genética dirigidaCombined small cell lung cancer (CSCLC)Small cell lung cancer (SCLC)Comprehensive genomic profilingTargeted gene sequencingComprehensive genomic profiling of combined small cell lung cancerComprehensive genomic profiling of combined small cell lung cancerArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Background: Combined small cell lung cancer (CSCLC) is an uncommon and heterogeneous subtype of small cell lung cancer (SCLC). However, there is limited data concerning the different molecular changes and clinical features in CSCLC compared to pure SCLC. Methods: The clinical and pathological characteristics of pure SCLC and CSCLC patients were analyzed. Immunohistochemistry and microdissection were performed to isolate the CSCLC components. Further molecular analysis was carried out by next-generation sequencing (NGS) in 12 CSCLC and 30 pure SCLC. Results: There were no significant differences in clinical features between CSCLC and pure SCLC. Overall survival (OS) of CSCLC patients was worse than pure SCLC (P=0.005). NGS results indicated that TP53 and RB1 were the most frequently mutated genes in both CSCLC (83.33% and 66.67%) and pure SCLC (80.00% and 63.33%) groups. However, less than 10% common mutations were found in both CSCLC and pure SCLC. When analyzing the data of SCLC and non-small cell lung cancer (NSCLC) components of CSCLC, more than 50% common mutations, and identical genes with mutations were detected. Moreover, there were also common biological processes and signaling pathways identified in CSCLC and pure SCLC, in addition to SCLC and NSCLC components. Conclusions: There were no significant differences in terms of clinical features between CSCLC and pure SCLC. However, the prognosis for CSCLC was worse than pure SCLC. NGS analysis suggested that CSCLC components might derive from the same pluripotent single clone with common initial molecular alterations and subsequent acquisitions of other genetic mutations.Acceso cerradohttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessORIGINALZhang_Jing_2021.pdfZhang_Jing_2021.pdfapplication/pdf1951716https://repositorio.unbosque.edu.co/bitstreams/8b05b633-6b07-42d1-a8c5-107b88896df4/download011d1a09c8608ec9745f148c97dd49a8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/2eee71a7-6918-4ac5-a2e7-bd9a1d4d6843/download8a4605be74aa9ea9d79846c1fba20a33MD53THUMBNAILZhang_Jing_2021.pdf.jpgZhang_Jing_2021.pdf.jpgIM Thumbnailimage/jpeg10303https://repositorio.unbosque.edu.co/bitstreams/811d3be8-1a58-4c38-9bae-9036125c150a/downloadd3c14bd7d2f534bfd20b016069c57892MD54TEXTZhang_Jing_2021.pdf.txtZhang_Jing_2021.pdf.txtExtracted texttext/plain70968https://repositorio.unbosque.edu.co/bitstreams/41f801d2-614d-41f6-82ae-45790d545fe2/download850077458456987521e80311ff44f271MD5520.500.12495/8686oai:repositorio.unbosque.edu.co:20.500.12495/86862024-02-06 23:45:39.419restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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