Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926

Endothelial activation and alteration during dengue virus (DENV) infection are multifactorial events; however, the role of extracellular vesicles (EVs) in these phenomena is not known. In the present study, we characterized the EVs released by DENV-2 infected U937 macrophage cell line and evaluated...

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Autores:
Velandia-Romero, Myriam Lucía
Calderón Peláez, María Angélica
Marquez-Ortiz, Ricaurte Alejandro
Madroñero, Leidy Johana
Barreto, Alfonso
Castellanos, Jaime
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/2001
Acceso en línea:
http://hdl.handle.net/20.500.12495/2001
https://doi.org/10.1371/journal.pone.0227030
Palabra clave:
Dengue
Exosomas
Macrófagos
Rights
License
Attribution 4.0 International
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network_acronym_str UNBOSQUE2
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dc.title.spa.fl_str_mv Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
dc.title.translated.none.fl_str_mv Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
title Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
spellingShingle Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
Dengue
Exosomas
Macrófagos
title_short Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
title_full Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
title_fullStr Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
title_full_unstemmed Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
title_sort Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
dc.creator.fl_str_mv Velandia-Romero, Myriam Lucía
Calderón Peláez, María Angélica
Marquez-Ortiz, Ricaurte Alejandro
Madroñero, Leidy Johana
Barreto, Alfonso
Castellanos, Jaime
dc.contributor.author.none.fl_str_mv Velandia-Romero, Myriam Lucía
Calderón Peláez, María Angélica
Marquez-Ortiz, Ricaurte Alejandro
Madroñero, Leidy Johana
Barreto, Alfonso
Castellanos, Jaime
dc.contributor.orcid.none.fl_str_mv Castellanos, Jaime [0000-0003-1596-8383]
Velandia-Romero, Myriam Lucía [0000-0002-3340-7304]
Calderón Peláez, María Angélica [0000-0003-0788-0795]
dc.subject.decs.spa.fl_str_mv Dengue
Exosomas
Macrófagos
topic Dengue
Exosomas
Macrófagos
description Endothelial activation and alteration during dengue virus (DENV) infection are multifactorial events; however, the role of extracellular vesicles (EVs) in these phenomena is not known. In the present study, we characterized the EVs released by DENV-2 infected U937 macrophage cell line and evaluated the changes in the physiology and integrity of the EA.hy926 endothelial cells exposed to them. U937 macrophages were infected, supernatants were collected, and EVs were purified and characterized. Then, polarized endothelial EA.hy926 cells were exposed to the EVs for 24 h, and the transendothelial electrical resistance (TEER), monolayer permeability, and the expression of tight junction and adhesion proteins and cytokines were evaluated. The isolated EVs from infected macrophages corresponded to exosomes and apoptotic bodies, which contained the viral NS3 protein and different miRs, among other products. Exposure of EA.hy926 cells to EVs induced an increase in TEER, as well as changes in the expression of VE-cadherin and ICAM in addition leads to an increase in TNF-α, IP-10, IL-10, RANTES, and MCP-1 secretion. These results suggest that the EVs of infected macrophages transport proteins and miR that induce early changes in the physiology of the endothelium, leading to its activation and eliciting a defense program against damage during first stages of the disease, even in the absence of the virus.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-03-06T23:52:13Z
dc.date.available.none.fl_str_mv 2020-03-06T23:52:13Z
dc.date.issued.none.fl_str_mv 2020
dc.type.spa.fl_str_mv article
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dc.type.local.spa.fl_str_mv artículo
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/2001
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1371/journal.pone.0227030
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
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identifier_str_mv 1932-6203
instname:Universidad El Bosque
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url http://hdl.handle.net/20.500.12495/2001
https://doi.org/10.1371/journal.pone.0227030
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv PLoS ONE, 1932-6203, Vol. 15, Nro. 1, 2020, p. 1-25
dc.relation.uri.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0227030
dc.rights.*.fl_str_mv Attribution 4.0 International
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dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.local.spa.fl_str_mv Acceso abierto
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dc.rights.creativecommons.none.fl_str_mv 2020
rights_invalid_str_mv Attribution 4.0 International
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dc.publisher.spa.fl_str_mv Public Library of Science
dc.publisher.journal.spa.fl_str_mv PLoS ONE
institution Universidad El Bosque
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spelling Velandia-Romero, Myriam LucíaCalderón Peláez, María AngélicaMarquez-Ortiz, Ricaurte AlejandroMadroñero, Leidy JohanaBarreto, AlfonsoCastellanos, JaimeCastellanos, Jaime [0000-0003-1596-8383]Velandia-Romero, Myriam Lucía [0000-0002-3340-7304]Calderón Peláez, María Angélica [0000-0003-0788-0795]2020-03-06T23:52:13Z2020-03-06T23:52:13Z20201932-6203http://hdl.handle.net/20.500.12495/2001https://doi.org/10.1371/journal.pone.0227030instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengPublic Library of SciencePLoS ONEPLoS ONE, 1932-6203, Vol. 15, Nro. 1, 2020, p. 1-25https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0227030Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Acceso abiertohttp://purl.org/coar/access_right/c_abf3412020http://purl.org/coar/access_right/c_abf2Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926articleartículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501DengueExosomasMacrófagosEndothelial activation and alteration during dengue virus (DENV) infection are multifactorial events; however, the role of extracellular vesicles (EVs) in these phenomena is not known. In the present study, we characterized the EVs released by DENV-2 infected U937 macrophage cell line and evaluated the changes in the physiology and integrity of the EA.hy926 endothelial cells exposed to them. U937 macrophages were infected, supernatants were collected, and EVs were purified and characterized. Then, polarized endothelial EA.hy926 cells were exposed to the EVs for 24 h, and the transendothelial electrical resistance (TEER), monolayer permeability, and the expression of tight junction and adhesion proteins and cytokines were evaluated. The isolated EVs from infected macrophages corresponded to exosomes and apoptotic bodies, which contained the viral NS3 protein and different miRs, among other products. Exposure of EA.hy926 cells to EVs induced an increase in TEER, as well as changes in the expression of VE-cadherin and ICAM in addition leads to an increase in TNF-α, IP-10, IL-10, RANTES, and MCP-1 secretion. 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