Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis
The objective of this study was to investigate clonal relationship among Porphyromonas gingivalis isolated from subgingival plaque and blood samples in positive transient bacteremia subjects with periodontitis. Unrelated patients with general chronic periodontitis or general aggressive periodontitis...
- Autores:
-
Pérez‐Chaparro, P. Juliana
Gracieux, Patrice
Lafaurie, Gloria Ines
Donnio, Pierre‐Yves
Bonnaure‐Mallet, Martine
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2008
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/5252
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/5252
https://doi.org/10.1111/j.1600-051X.2008.01296.x
https://repositorio.unbosque.edu.co
- Palabra clave:
- Bacteremia
Genetics
Periodontitis
Porphyromonas gingivalis
Pulsed field gel electrophoresis
- Rights
- openAccess
- License
- Acceso abierto
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dc.title.spa.fl_str_mv |
Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis |
dc.title.translated.spa.fl_str_mv |
Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis |
title |
Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis |
spellingShingle |
Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis Bacteremia Genetics Periodontitis Porphyromonas gingivalis Pulsed field gel electrophoresis |
title_short |
Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis |
title_full |
Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis |
title_fullStr |
Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis |
title_full_unstemmed |
Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis |
title_sort |
Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis |
dc.creator.fl_str_mv |
Pérez‐Chaparro, P. Juliana Gracieux, Patrice Lafaurie, Gloria Ines Donnio, Pierre‐Yves Bonnaure‐Mallet, Martine |
dc.contributor.author.none.fl_str_mv |
Pérez‐Chaparro, P. Juliana Gracieux, Patrice Lafaurie, Gloria Ines Donnio, Pierre‐Yves Bonnaure‐Mallet, Martine |
dc.contributor.orcid.none.fl_str_mv |
Lafaurie, Gloria Ines [0000-0003-3986-0625] |
dc.subject.keywords.spa.fl_str_mv |
Bacteremia Genetics Periodontitis Porphyromonas gingivalis Pulsed field gel electrophoresis |
topic |
Bacteremia Genetics Periodontitis Porphyromonas gingivalis Pulsed field gel electrophoresis |
description |
The objective of this study was to investigate clonal relationship among Porphyromonas gingivalis isolated from subgingival plaque and blood samples in positive transient bacteremia subjects with periodontitis. Unrelated patients with general chronic periodontitis or general aggressive periodontitis requiring scaling and root planing (SRP) were included in the study. Genotyping of each isolate was performed using pulsed field gel electrophoresis technique. Genetic relatedness of strains isolated within an individual or between different patients was determined by dendogram analysis. Following SRP, from 16 patients, seven patients showed positive P. gingivalis bacteremia and nine were negative. Thirty‐two strains were isolated from subgingival plaque and blood samples before and during induced transient bacteremia. The majority of the patients harboured one clonal type. Two patients showed different clones in plaque and blood samples suggesting that more than one clone can be found in subgingival plaque. P. gingivalis isolates from periodontitis patients after transient bacteremia following SRP, revealed a high heterogeneity among isolates. In 6/16 subjects the same P. gingivalis isolate was found in the blood and in oral cavity. P. gingivalis heterogeneity suggests no association of a unique clonal type with transient bacteremia. |
publishDate |
2008 |
dc.date.issued.none.fl_str_mv |
2008 |
dc.date.accessioned.none.fl_str_mv |
2021-02-05T16:14:04Z |
dc.date.available.none.fl_str_mv |
2021-02-05T16:14:04Z |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.none.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.issn.none.fl_str_mv |
1600-051X |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/5252 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1111/j.1600-051X.2008.01296.x |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
https://repositorio.unbosque.edu.co |
identifier_str_mv |
1600-051X instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque |
url |
http://hdl.handle.net/20.500.12495/5252 https://doi.org/10.1111/j.1600-051X.2008.01296.x https://repositorio.unbosque.edu.co |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Journal of Clinical Periodontology, 1600-051X, Vol. 35, Nro. 9, 2008 p. 748-753 |
dc.relation.uri.none.fl_str_mv |
https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-051X.2008.01296.x |
dc.rights.local.spa.fl_str_mv |
Acceso abierto |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Acceso abierto |
dc.rights.creativecommons.none.fl_str_mv |
2008 |
rights_invalid_str_mv |
Acceso abierto http://purl.org/coar/access_right/c_abf2 2008 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Wiley |
dc.publisher.journal.spa.fl_str_mv |
Journal of Clinical Periodontology |
institution |
Universidad El Bosque |
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Pérez‐Chaparro, P. JulianaGracieux, PatriceLafaurie, Gloria InesDonnio, Pierre‐YvesBonnaure‐Mallet, MartineLafaurie, Gloria Ines [0000-0003-3986-0625]2021-02-05T16:14:04Z2021-02-05T16:14:04Z20081600-051Xhttp://hdl.handle.net/20.500.12495/5252https://doi.org/10.1111/j.1600-051X.2008.01296.xinstname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengWileyJournal of Clinical PeriodontologyJournal of Clinical Periodontology, 1600-051X, Vol. 35, Nro. 9, 2008 p. 748-753https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-051X.2008.01296.xGenotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitisGenotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitisArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85BacteremiaGeneticsPeriodontitisPorphyromonas gingivalisPulsed field gel electrophoresisThe objective of this study was to investigate clonal relationship among Porphyromonas gingivalis isolated from subgingival plaque and blood samples in positive transient bacteremia subjects with periodontitis. Unrelated patients with general chronic periodontitis or general aggressive periodontitis requiring scaling and root planing (SRP) were included in the study. Genotyping of each isolate was performed using pulsed field gel electrophoresis technique. Genetic relatedness of strains isolated within an individual or between different patients was determined by dendogram analysis. Following SRP, from 16 patients, seven patients showed positive P. gingivalis bacteremia and nine were negative. Thirty‐two strains were isolated from subgingival plaque and blood samples before and during induced transient bacteremia. The majority of the patients harboured one clonal type. Two patients showed different clones in plaque and blood samples suggesting that more than one clone can be found in subgingival plaque. P. gingivalis isolates from periodontitis patients after transient bacteremia following SRP, revealed a high heterogeneity among isolates. In 6/16 subjects the same P. gingivalis isolate was found in the blood and in oral cavity. P. gingivalis heterogeneity suggests no association of a unique clonal type with transient bacteremia.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2008ORIGINALPérez_Chaparro_P._Juliana_2008.pdfPérez_Chaparro_P._Juliana_2008.pdfapplication/pdf295827https://repositorio.unbosque.edu.co/bitstreams/d1f2e1d8-6c59-44e0-b2e4-ac11eecb8981/downloadfeda0a43f398ead8d66dccae253b9942MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/94fe7e22-238e-4d38-af34-22b6592fce2f/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILPérez_Chaparro_P._Juliana_2008.jpgPérez_Chaparro_P._Juliana_2008.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/ef4e702e-4ecb-41af-a3df-a337e76b10f7/download7210a811635d1799e7c05fee5d259be7MD53Pérez_Chaparro_P._Juliana_2008.pdf.jpgPérez_Chaparro_P._Juliana_2008.pdf.jpgIM Thumbnailimage/jpeg12800https://repositorio.unbosque.edu.co/bitstreams/f0ab79a2-59e0-45b6-8b7d-3811878d7802/download0e813b486373b0243d9f586a4e09f0c6MD54TEXTPérez_Chaparro_P._Juliana_2008.pdf.txtPérez_Chaparro_P._Juliana_2008.pdf.txtExtracted texttext/plain32920https://repositorio.unbosque.edu.co/bitstreams/8c2af12d-8680-49c3-a50d-01a2260e0a13/downloadf2e64900c009047f653187f922299f8fMD5520.500.12495/5252oai:repositorio.unbosque.edu.co:20.500.12495/52522024-02-06 22:47:25.509restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |