Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis

The objective of this study was to investigate clonal relationship among Porphyromonas gingivalis isolated from subgingival plaque and blood samples in positive transient bacteremia subjects with periodontitis. Unrelated patients with general chronic periodontitis or general aggressive periodontitis...

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Autores:
Pérez‐Chaparro, P. Juliana
Gracieux, Patrice
Lafaurie, Gloria Ines
Donnio, Pierre‐Yves
Bonnaure‐Mallet, Martine
Tipo de recurso:
Article of journal
Fecha de publicación:
2008
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/5252
Acceso en línea:
http://hdl.handle.net/20.500.12495/5252
https://doi.org/10.1111/j.1600-051X.2008.01296.x
https://repositorio.unbosque.edu.co
Palabra clave:
Bacteremia
Genetics
Periodontitis
Porphyromonas gingivalis
Pulsed field gel electrophoresis
Rights
openAccess
License
Acceso abierto
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repository_id_str
dc.title.spa.fl_str_mv Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis
dc.title.translated.spa.fl_str_mv Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis
title Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis
spellingShingle Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis
Bacteremia
Genetics
Periodontitis
Porphyromonas gingivalis
Pulsed field gel electrophoresis
title_short Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis
title_full Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis
title_fullStr Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis
title_full_unstemmed Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis
title_sort Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis
dc.creator.fl_str_mv Pérez‐Chaparro, P. Juliana
Gracieux, Patrice
Lafaurie, Gloria Ines
Donnio, Pierre‐Yves
Bonnaure‐Mallet, Martine
dc.contributor.author.none.fl_str_mv Pérez‐Chaparro, P. Juliana
Gracieux, Patrice
Lafaurie, Gloria Ines
Donnio, Pierre‐Yves
Bonnaure‐Mallet, Martine
dc.contributor.orcid.none.fl_str_mv Lafaurie, Gloria Ines [0000-0003-3986-0625]
dc.subject.keywords.spa.fl_str_mv Bacteremia
Genetics
Periodontitis
Porphyromonas gingivalis
Pulsed field gel electrophoresis
topic Bacteremia
Genetics
Periodontitis
Porphyromonas gingivalis
Pulsed field gel electrophoresis
description The objective of this study was to investigate clonal relationship among Porphyromonas gingivalis isolated from subgingival plaque and blood samples in positive transient bacteremia subjects with periodontitis. Unrelated patients with general chronic periodontitis or general aggressive periodontitis requiring scaling and root planing (SRP) were included in the study. Genotyping of each isolate was performed using pulsed field gel electrophoresis technique. Genetic relatedness of strains isolated within an individual or between different patients was determined by dendogram analysis. Following SRP, from 16 patients, seven patients showed positive P. gingivalis bacteremia and nine were negative. Thirty‐two strains were isolated from subgingival plaque and blood samples before and during induced transient bacteremia. The majority of the patients harboured one clonal type. Two patients showed different clones in plaque and blood samples suggesting that more than one clone can be found in subgingival plaque. P. gingivalis isolates from periodontitis patients after transient bacteremia following SRP, revealed a high heterogeneity among isolates. In 6/16 subjects the same P. gingivalis isolate was found in the blood and in oral cavity. P. gingivalis heterogeneity suggests no association of a unique clonal type with transient bacteremia.
publishDate 2008
dc.date.issued.none.fl_str_mv 2008
dc.date.accessioned.none.fl_str_mv 2021-02-05T16:14:04Z
dc.date.available.none.fl_str_mv 2021-02-05T16:14:04Z
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dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.none.fl_str_mv Artículo de revista
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dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.issn.none.fl_str_mv 1600-051X
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/5252
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1111/j.1600-051X.2008.01296.x
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
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identifier_str_mv 1600-051X
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url http://hdl.handle.net/20.500.12495/5252
https://doi.org/10.1111/j.1600-051X.2008.01296.x
https://repositorio.unbosque.edu.co
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Journal of Clinical Periodontology, 1600-051X, Vol. 35, Nro. 9, 2008 p. 748-753
dc.relation.uri.none.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-051X.2008.01296.x
dc.rights.local.spa.fl_str_mv Acceso abierto
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
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dc.rights.creativecommons.none.fl_str_mv 2008
rights_invalid_str_mv Acceso abierto
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2008
eu_rights_str_mv openAccess
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dc.publisher.spa.fl_str_mv Wiley
dc.publisher.journal.spa.fl_str_mv Journal of Clinical Periodontology
institution Universidad El Bosque
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spelling Pérez‐Chaparro, P. JulianaGracieux, PatriceLafaurie, Gloria InesDonnio, Pierre‐YvesBonnaure‐Mallet, MartineLafaurie, Gloria Ines [0000-0003-3986-0625]2021-02-05T16:14:04Z2021-02-05T16:14:04Z20081600-051Xhttp://hdl.handle.net/20.500.12495/5252https://doi.org/10.1111/j.1600-051X.2008.01296.xinstname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengWileyJournal of Clinical PeriodontologyJournal of Clinical Periodontology, 1600-051X, Vol. 35, Nro. 9, 2008 p. 748-753https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-051X.2008.01296.xGenotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitisGenotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitisArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85BacteremiaGeneticsPeriodontitisPorphyromonas gingivalisPulsed field gel electrophoresisThe objective of this study was to investigate clonal relationship among Porphyromonas gingivalis isolated from subgingival plaque and blood samples in positive transient bacteremia subjects with periodontitis. Unrelated patients with general chronic periodontitis or general aggressive periodontitis requiring scaling and root planing (SRP) were included in the study. Genotyping of each isolate was performed using pulsed field gel electrophoresis technique. Genetic relatedness of strains isolated within an individual or between different patients was determined by dendogram analysis. Following SRP, from 16 patients, seven patients showed positive P. gingivalis bacteremia and nine were negative. Thirty‐two strains were isolated from subgingival plaque and blood samples before and during induced transient bacteremia. The majority of the patients harboured one clonal type. Two patients showed different clones in plaque and blood samples suggesting that more than one clone can be found in subgingival plaque. P. gingivalis isolates from periodontitis patients after transient bacteremia following SRP, revealed a high heterogeneity among isolates. In 6/16 subjects the same P. gingivalis isolate was found in the blood and in oral cavity. 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