Exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar

Enterococcus faecalis is a commensal bacterium of the gastrointestinal tract that can cause nosocomial infections in immunocompromised humans. The hallmarks of this organism are its ability to survive in a variety of stressful habitats and, in particular, its ability to withstand membrane damage. On...

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Autores:
Harp, John R.
Saito, Holly E.
Bourdon, Allen K.
Reyes, Jinnethe
Arias, Cesar A.
Campagna, Shawn R.
Fozo, Elizabeth M.
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/3622
Acceso en línea:
http://hdl.handle.net/20.500.12495/3622
https://repositorio.unbosque.edu.co
Palabra clave:
Ácidos grasos
Enterococcus faecalis
Daptomicina
Rights
openAccess
License
Acceso abierto
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dc.title.spa.fl_str_mv Exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar
dc.title.translated.spa.fl_str_mv Exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar
title Exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar
spellingShingle Exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar
Ácidos grasos
Enterococcus faecalis
Daptomicina
title_short Exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar
title_full Exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar
title_fullStr Exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar
title_full_unstemmed Exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar
title_sort Exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar
dc.creator.fl_str_mv Harp, John R.
Saito, Holly E.
Bourdon, Allen K.
Reyes, Jinnethe
Arias, Cesar A.
Campagna, Shawn R.
Fozo, Elizabeth M.
dc.contributor.author.none.fl_str_mv Harp, John R.
Saito, Holly E.
Bourdon, Allen K.
Reyes, Jinnethe
Arias, Cesar A.
Campagna, Shawn R.
Fozo, Elizabeth M.
dc.subject.decs.spa.fl_str_mv Ácidos grasos
Enterococcus faecalis
Daptomicina
topic Ácidos grasos
Enterococcus faecalis
Daptomicina
description Enterococcus faecalis is a commensal bacterium of the gastrointestinal tract that can cause nosocomial infections in immunocompromised humans. The hallmarks of this organism are its ability to survive in a variety of stressful habitats and, in particular, its ability to withstand membrane damage. One strategy used by E. faecalis to protect itself from membrane-damaging agents, including the antibiotic daptomycin, involves incorporation of exogenous fatty acids from bile or serum into the cell membrane. Additionally, the response regulator LiaR (a member of the LiaFSR [lipid II-interacting antibiotic response regulator and sensor] system associated with cell envelope stress responses) is required for the basal level of resistance E. faecalis has to daptomycin-induced membrane damage. This study aimed to determine if membrane fatty acid changes could provide protection against membrane stressors in a LiaR-deficient strain of E. faecalis. We noted that despite the loss of LiaR, the organism readily incorporated exogenous fatty acids into its membrane, and indeed growth in the presence of exogenous fatty acids increased the survival of LiaR-deficient cells when challenged with a variety of membrane stressors, including daptomycin. Combined, our results suggest that E. faecalis can utilize both LiaR-dependent and -independent mechanisms to protect itself from membrane damage. IMPORTANCE Enterococcus faecalis is responsible for a significant number of nosocomial infections. Worse, many of the antibiotics used to treat E. faecalis infection are no longer effective, as this organism has developed resistance to them. The drug daptomycin has been successfully used to treat some of these resistant strains; however, daptomycin-resistant isolates have been identified in hospitals. Many daptomycin-resistant isolates are found to harbor mutations in the genetic locus liaFSR, which is involved in membrane stress responses. Another mechanism shown to increase tolerance to daptomycin involves the incorporation of exogenous fatty acids from host fluids like serum or bile. This improved tolerance was found to be independent of liaFSR and suggests that there are additional ways to impact sensitivity to daptomycin. Thus, further studies are needed to understand how host fatty acid sources can influence antibiotic susceptibility.
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2020-07-30T16:58:48Z
dc.date.available.none.fl_str_mv 2020-07-30T16:58:48Z
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dc.type.local.none.fl_str_mv Artículo de revista
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dc.identifier.issn.none.fl_str_mv 1098-5336
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/3622
dc.identifier.doi.none.fl_str_mv 10.1128/AEM.00933-16
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
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dc.language.iso.none.fl_str_mv eng
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dc.relation.ispartofseries.spa.fl_str_mv Applied and environmental microbiology, 1098-5336. Vol 82, Nro 14, 2016, p. 4410-4420
dc.relation.uri.none.fl_str_mv https://aem.asm.org/content/82/14/4410.long
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2016-07
eu_rights_str_mv openAccess
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dc.publisher.spa.fl_str_mv American Society for Microbiology
dc.publisher.journal.spa.fl_str_mv Applied and environmental microbiology
institution Universidad El Bosque
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spelling Harp, John R.Saito, Holly E.Bourdon, Allen K.Reyes, JinnetheArias, Cesar A.Campagna, Shawn R.Fozo, Elizabeth M.2020-07-30T16:58:48Z2020-07-30T16:58:48Z20161098-5336http://hdl.handle.net/20.500.12495/362210.1128/AEM.00933-16instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengAmerican Society for MicrobiologyApplied and environmental microbiologyApplied and environmental microbiology, 1098-5336. Vol 82, Nro 14, 2016, p. 4410-4420https://aem.asm.org/content/82/14/4410.longExogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liarExogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liarArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Ácidos grasosEnterococcus faecalisDaptomicinaEnterococcus faecalis is a commensal bacterium of the gastrointestinal tract that can cause nosocomial infections in immunocompromised humans. The hallmarks of this organism are its ability to survive in a variety of stressful habitats and, in particular, its ability to withstand membrane damage. One strategy used by E. faecalis to protect itself from membrane-damaging agents, including the antibiotic daptomycin, involves incorporation of exogenous fatty acids from bile or serum into the cell membrane. Additionally, the response regulator LiaR (a member of the LiaFSR [lipid II-interacting antibiotic response regulator and sensor] system associated with cell envelope stress responses) is required for the basal level of resistance E. faecalis has to daptomycin-induced membrane damage. This study aimed to determine if membrane fatty acid changes could provide protection against membrane stressors in a LiaR-deficient strain of E. faecalis. We noted that despite the loss of LiaR, the organism readily incorporated exogenous fatty acids into its membrane, and indeed growth in the presence of exogenous fatty acids increased the survival of LiaR-deficient cells when challenged with a variety of membrane stressors, including daptomycin. Combined, our results suggest that E. faecalis can utilize both LiaR-dependent and -independent mechanisms to protect itself from membrane damage. IMPORTANCE Enterococcus faecalis is responsible for a significant number of nosocomial infections. Worse, many of the antibiotics used to treat E. faecalis infection are no longer effective, as this organism has developed resistance to them. The drug daptomycin has been successfully used to treat some of these resistant strains; however, daptomycin-resistant isolates have been identified in hospitals. Many daptomycin-resistant isolates are found to harbor mutations in the genetic locus liaFSR, which is involved in membrane stress responses. Another mechanism shown to increase tolerance to daptomycin involves the incorporation of exogenous fatty acids from host fluids like serum or bile. This improved tolerance was found to be independent of liaFSR and suggests that there are additional ways to impact sensitivity to daptomycin. Thus, further studies are needed to understand how host fatty acid sources can influence antibiotic susceptibility.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2016-07ORIGINALHarp, John R..pdfHarp, John R..pdfapplication/pdf1594178https://repositorio.unbosque.edu.co/bitstreams/97a1471b-fa9a-46fb-9ee5-344a56f2b357/download3556e76efda7cbe328146d87bab88e4fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/ad17a9c0-8f89-4c6e-8a8f-d8de81de5051/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILHarp, John R..pdf.jpgHarp, John R..pdf.jpgIM Thumbnailimage/jpeg14241https://repositorio.unbosque.edu.co/bitstreams/56958442-6b24-48a5-b11c-497339312c01/download20fb84aacfae9d0cf33656e2dc4c17f9MD53TEXTHarp, John R..pdf.txtHarp, John R..pdf.txtExtracted texttext/plain69055https://repositorio.unbosque.edu.co/bitstreams/452be00b-295b-4d8b-bebf-822aaab57e04/downloadbf3dd569b476a1f46fee6a7a2e8b4010MD5420.500.12495/3622oai:repositorio.unbosque.edu.co:20.500.12495/36222024-02-07 01:57:16.313open.accesshttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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