Evaluación de la actividad citotóxica y antioxidante de aceites derivados del fruto de seje (oenocarpus bataua) en un modelo in vitro de hepatocarcinoma humano

Actualmente la actividad antioxidante se ha convertido en un atributo altamente demandado en la industria alimentaria, farmacéutica y cosmética, razón por la cual se están buscando cada vez más compuestos que tengan un potencial efecto antioxidante. En este contexto, el fruto de la palma Oenocarpus...

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Autores:
Gaitán Blanco, Sara
Barrero Echeverria, Juan Pablo
Tipo de recurso:
https://purl.org/coar/resource_type/c_7a1f
Fecha de publicación:
2025
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
spa
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/14391
Acceso en línea:
https://hdl.handle.net/20.500.12495/14391
Palabra clave:
Antioxidantes
Aceites Esenciales
Especies Reactivas de Oxígeno
Estrés Oxidativo
615.19
Antioxidants
Essential Oils
Reactive Oxygen Species
Oxidative Stress
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Attribution-NonCommercial-NoDerivatives 4.0 International
id UNBOSQUE2_5c59962464de5cec304a7abfb00cd0ac
oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/14391
network_acronym_str UNBOSQUE2
network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.none.fl_str_mv Evaluación de la actividad citotóxica y antioxidante de aceites derivados del fruto de seje (oenocarpus bataua) en un modelo in vitro de hepatocarcinoma humano
dc.title.translated.none.fl_str_mv Evaluation of the cytotoxic and antioxidant activity of oils derived from seje fruit (oenocarpus bataua) in an in vitro model of human hepatocarcinoma
title Evaluación de la actividad citotóxica y antioxidante de aceites derivados del fruto de seje (oenocarpus bataua) en un modelo in vitro de hepatocarcinoma humano
spellingShingle Evaluación de la actividad citotóxica y antioxidante de aceites derivados del fruto de seje (oenocarpus bataua) en un modelo in vitro de hepatocarcinoma humano
Antioxidantes
Aceites Esenciales
Especies Reactivas de Oxígeno
Estrés Oxidativo
615.19
Antioxidants
Essential Oils
Reactive Oxygen Species
Oxidative Stress
title_short Evaluación de la actividad citotóxica y antioxidante de aceites derivados del fruto de seje (oenocarpus bataua) en un modelo in vitro de hepatocarcinoma humano
title_full Evaluación de la actividad citotóxica y antioxidante de aceites derivados del fruto de seje (oenocarpus bataua) en un modelo in vitro de hepatocarcinoma humano
title_fullStr Evaluación de la actividad citotóxica y antioxidante de aceites derivados del fruto de seje (oenocarpus bataua) en un modelo in vitro de hepatocarcinoma humano
title_full_unstemmed Evaluación de la actividad citotóxica y antioxidante de aceites derivados del fruto de seje (oenocarpus bataua) en un modelo in vitro de hepatocarcinoma humano
title_sort Evaluación de la actividad citotóxica y antioxidante de aceites derivados del fruto de seje (oenocarpus bataua) en un modelo in vitro de hepatocarcinoma humano
dc.creator.fl_str_mv Gaitán Blanco, Sara
Barrero Echeverria, Juan Pablo
dc.contributor.advisor.none.fl_str_mv Morantes Medina, Sandra Johanna
Ballesteros Vivas, Diego
dc.contributor.author.none.fl_str_mv Gaitán Blanco, Sara
Barrero Echeverria, Juan Pablo
dc.contributor.orcid.none.fl_str_mv Gaitan Blanco, Sara [0009-0005-0171-7657]
Barrero Echeverria, Juan Pablo [0009-0007-1605-1426]
dc.subject.none.fl_str_mv Antioxidantes
Aceites Esenciales
Especies Reactivas de Oxígeno
Estrés Oxidativo
topic Antioxidantes
Aceites Esenciales
Especies Reactivas de Oxígeno
Estrés Oxidativo
615.19
Antioxidants
Essential Oils
Reactive Oxygen Species
Oxidative Stress
dc.subject.ddc.none.fl_str_mv 615.19
dc.subject.keywords.none.fl_str_mv Antioxidants
Essential Oils
Reactive Oxygen Species
Oxidative Stress
description Actualmente la actividad antioxidante se ha convertido en un atributo altamente demandado en la industria alimentaria, farmacéutica y cosmética, razón por la cual se están buscando cada vez más compuestos que tengan un potencial efecto antioxidante. En este contexto, el fruto de la palma Oenocarpus bataua, más conocida como seje, se presenta como un candidato ideal ya que en anteriores estudios se ha demostrado actividad antioxidante, sin embargo, dichos estudios no proporcionan información en condiciones biológicas. En este sentido, el objetivo del presente trabajo fue evaluar en un modelo in vitro de hepatocarcinoma humano, la actividad citotóxica y antioxidante celular de aceites derivados del seje. Los aceites fueron extraídos mediante dos metodologías diferentes: soxhlet (SOX) y fluidos supercríticos (FSC), debido a la naturaleza oleosa del aceite fue necesario realizar una emulsificación con lecitina de soya y buffer de fosfatos. Los resultados del ensayo de citotoxicidad demostraron que las emulsiones SOX y FSC fueron seguras en todas las concentraciones evaluadas tras una exposición de 24 horas en células L929 y HepG2, observándose mayores porcentajes de viabilidad celular con la emulsión FSC en ambos modelos celulares. Posteriormente, se evaluó la actividad antioxidante celular (CAA) en la línea HepG2 empleando DCFH-DA. Como control positivo se utilizó quercetina, la cual mostró un efecto dosis-dependiente al reducir tanto los niveles basales de ROS como los inducidos por un prooxidante, en contraste, la emulsión FSC no mostró efecto antioxidante. Por otro lado, la emulsión extraída por Soxhlet sí presentó actividad antioxidante en el rango de concentraciones de 5000 - 1250 μg/mL. En conclusión, los ensayos realizados demuestran que, aunque el aceite extraído por FSC ofrece un perfil de seguridad superior, no demuestra un efecto antioxidante notable. Por el contrario, el aceite obtenido mediante Soxhlet garantiza la seguridad celular y reduce la producción inducida de ROS, posicionándose como una fuente potencial de compuestos bioactivos para diversas aplicaciones.
publishDate 2025
dc.date.accessioned.none.fl_str_mv 2025-05-19T17:12:45Z
dc.date.available.none.fl_str_mv 2025-05-19T17:12:45Z
dc.date.issued.none.fl_str_mv 2025-05
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.local.none.fl_str_mv Tesis/Trabajo de grado - Monografía - Pregrado
dc.type.coar.none.fl_str_mv https://purl.org/coar/resource_type/c_7a1f
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/bachelorThesis
dc.type.coarversion.none.fl_str_mv https://purl.org/coar/version/c_ab4af688f83e57aa
format https://purl.org/coar/resource_type/c_7a1f
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12495/14391
dc.identifier.instname.spa.fl_str_mv Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv repourl:https://repositorio.unbosque.edu.co
url https://hdl.handle.net/20.500.12495/14391
identifier_str_mv Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
repourl:https://repositorio.unbosque.edu.co
dc.language.iso.fl_str_mv spa
language spa
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spelling Morantes Medina, Sandra JohannaBallesteros Vivas, DiegoGaitán Blanco, SaraBarrero Echeverria, Juan PabloGaitan Blanco, Sara [0009-0005-0171-7657]Barrero Echeverria, Juan Pablo [0009-0007-1605-1426]2025-05-19T17:12:45Z2025-05-19T17:12:45Z2025-05https://hdl.handle.net/20.500.12495/14391Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coActualmente la actividad antioxidante se ha convertido en un atributo altamente demandado en la industria alimentaria, farmacéutica y cosmética, razón por la cual se están buscando cada vez más compuestos que tengan un potencial efecto antioxidante. En este contexto, el fruto de la palma Oenocarpus bataua, más conocida como seje, se presenta como un candidato ideal ya que en anteriores estudios se ha demostrado actividad antioxidante, sin embargo, dichos estudios no proporcionan información en condiciones biológicas. En este sentido, el objetivo del presente trabajo fue evaluar en un modelo in vitro de hepatocarcinoma humano, la actividad citotóxica y antioxidante celular de aceites derivados del seje. Los aceites fueron extraídos mediante dos metodologías diferentes: soxhlet (SOX) y fluidos supercríticos (FSC), debido a la naturaleza oleosa del aceite fue necesario realizar una emulsificación con lecitina de soya y buffer de fosfatos. Los resultados del ensayo de citotoxicidad demostraron que las emulsiones SOX y FSC fueron seguras en todas las concentraciones evaluadas tras una exposición de 24 horas en células L929 y HepG2, observándose mayores porcentajes de viabilidad celular con la emulsión FSC en ambos modelos celulares. Posteriormente, se evaluó la actividad antioxidante celular (CAA) en la línea HepG2 empleando DCFH-DA. Como control positivo se utilizó quercetina, la cual mostró un efecto dosis-dependiente al reducir tanto los niveles basales de ROS como los inducidos por un prooxidante, en contraste, la emulsión FSC no mostró efecto antioxidante. Por otro lado, la emulsión extraída por Soxhlet sí presentó actividad antioxidante en el rango de concentraciones de 5000 - 1250 μg/mL. En conclusión, los ensayos realizados demuestran que, aunque el aceite extraído por FSC ofrece un perfil de seguridad superior, no demuestra un efecto antioxidante notable. Por el contrario, el aceite obtenido mediante Soxhlet garantiza la seguridad celular y reduce la producción inducida de ROS, posicionándose como una fuente potencial de compuestos bioactivos para diversas aplicaciones.Pontificia Universidad JaverianaPregradoQuímico FarmacéuticoCurrently, antioxidant activity has become a highly sought-after attribute in the food, pharmaceutical, and cosmetic industries, which is why compounds with potential antioxidant effects are increasingly being sought. In this context, the fruit of the palm Oenocarpus bataua, better known as seje, is presented as an ideal candidate since previous studies have demonstrated antioxidant activity; however, these studies do not provide information under biological conditions. In this sense, the objective of the present study was to evaluate the cellular cytotoxic and antioxidant activity of seje-derived oils in an in vitro model of human hepatocarcinoma. The oils were extracted using two different methodologies: Soxhlet extraction (SOX) and supercritical fluid extraction (SCF). Due to the oil's oily nature, emulsification with soy lecithin and phosphate buffer was necessary. The results of the cytotoxicity assay demonstrated that SOX and FSC emulsions were safe at all concentrations tested after a 24-hour exposure to L929 and HepG2 cells, with higher percentages of cell viability observed with the FSC emulsion in both cell models. Cellular antioxidant activity (CAA) was subsequently evaluated in the HepG2 cell line using DCFH-DA. Quercetin was used as a positive control, showing a dose-dependent effect by reducing both basal and pro-oxidant-induced ROS levels; in contrast, the FSC emulsion did not exhibit an antioxidant effect. On the other hand, the Soxhlet-extracted emulsion did exhibit antioxidant activity in the concentration range of 5000–1250 μg/mL. In conclusion, the assays performed demonstrate that, although the FSC-extracted oil offers a superior safety profile, it does not demonstrate a notable antioxidant effect. In contrast, the oil obtained by Soxhlet ensures cellular safety and reduces induced ROS production, positioning itself as a potential source of bioactive compounds for various applications.application/pdfAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertohttps://purl.org/coar/access_right/c_abf2http://purl.org/coar/access_right/c_abf2AntioxidantesAceites EsencialesEspecies Reactivas de OxígenoEstrés Oxidativo615.19AntioxidantsEssential OilsReactive Oxygen SpeciesOxidative StressEvaluación de la actividad citotóxica y antioxidante de aceites derivados del fruto de seje (oenocarpus bataua) en un modelo in vitro de hepatocarcinoma humanoEvaluation of the cytotoxic and antioxidant activity of oils derived from seje fruit (oenocarpus bataua) in an in vitro model of human hepatocarcinomaQuímica FarmacéuticaUniversidad El BosqueFacultad de CienciasTesis/Trabajo de grado - Monografía - Pregradohttps://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesishttps://purl.org/coar/version/c_ab4af688f83e57aa1. 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