Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance

Aiming to develop biological skin dresses with improved performance in the treatment of skin wounds, acellular collagen I scaffolds were modified with polymeric microparticles and the subsequent loading of a hydroglycolic extract of Calendula officinalis flowers. Microparticles made of gelatin-colla...

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Autores:
Jiménez, Ronald A.
Millán, Diana
Suesca, Edward
Sosnik, Alejandro
Fontanilla, Marta R.
Tipo de recurso:
Article of journal
Fecha de publicación:
2015
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/3714
Acceso en línea:
http://hdl.handle.net/20.500.12495/3714
https://doi.org//10.1007/s13346-015-0217-3
https://repositorio.unbosque.edu.co
Palabra clave:
Wound healing
Collagen I scaffolds
Calendula officinalis L
Flowers extract
Polyphenols
Gelatin-collagen
Microparticle-loaded skin dressings
Rights
closedAccess
License
Acceso cerrado
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oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/3714
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network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.spa.fl_str_mv Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance
dc.title.translated.spa.fl_str_mv Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance
title Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance
spellingShingle Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance
Wound healing
Collagen I scaffolds
Calendula officinalis L
Flowers extract
Polyphenols
Gelatin-collagen
Microparticle-loaded skin dressings
title_short Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance
title_full Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance
title_fullStr Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance
title_full_unstemmed Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance
title_sort Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance
dc.creator.fl_str_mv Jiménez, Ronald A.
Millán, Diana
Suesca, Edward
Sosnik, Alejandro
Fontanilla, Marta R.
dc.contributor.author.none.fl_str_mv Jiménez, Ronald A.
Millán, Diana
Suesca, Edward
Sosnik, Alejandro
Fontanilla, Marta R.
dc.subject.keywords.spa.fl_str_mv Wound healing
Collagen I scaffolds
Calendula officinalis L
Flowers extract
Polyphenols
Gelatin-collagen
Microparticle-loaded skin dressings
topic Wound healing
Collagen I scaffolds
Calendula officinalis L
Flowers extract
Polyphenols
Gelatin-collagen
Microparticle-loaded skin dressings
description Aiming to develop biological skin dresses with improved performance in the treatment of skin wounds, acellular collagen I scaffolds were modified with polymeric microparticles and the subsequent loading of a hydroglycolic extract of Calendula officinalis flowers. Microparticles made of gelatin-collagen were produced by a water-in-oil emulsion/cross-linking method. Thereafter, these microparticles were mixed with collagen suspensions at three increasing concentrations and the resulting mixtures lyophilized to make microparticle-loaded porous collagen scaffolds. Resistance to enzymatic degradation, ability to associate with the C. officinalis extract, and the extract release profile of the three gelatin-collagen microparticle-scaffold prototypes were assessed in vitro and compared to collagen scaffolds without microparticles used as control. Data indicated that the incorporation of gelatin-collagen microparticles increased the resistance of the scaffolds to in vitro enzymatic degradation, as well as their association with the C. officinalis flower extract. In addition, a sharp decrease in cytotoxicity, as well as more prolonged release of the extract, was attained. Overall results support the potential of these systems to develop innovative dermal substitutes with improved features. Furthermore, the gelatin-collagen mixture represents a low-cost and scalable alternative with high clinical transferability, especially appealing in developing countries.
publishDate 2015
dc.date.issued.none.fl_str_mv 2015
dc.date.accessioned.none.fl_str_mv 2020-08-06T19:07:54Z
dc.date.available.none.fl_str_mv 2020-08-06T19:07:54Z
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dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.none.fl_str_mv Artículo de revista
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dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.issn.none.fl_str_mv 2190-3948
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/3714
dc.identifier.doi.none.fl_str_mv https://doi.org//10.1007/s13346-015-0217-3
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv https://repositorio.unbosque.edu.co
identifier_str_mv 2190-3948
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
url http://hdl.handle.net/20.500.12495/3714
https://doi.org//10.1007/s13346-015-0217-3
https://repositorio.unbosque.edu.co
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Drug Delivery and Translational Research, 2190-3948, Vol. 5, 2015, p. 209–218
dc.relation.uri.none.fl_str_mv https://link.springer.com/article/10.1007%2Fs13346-015-0217-3
dc.rights.local.spa.fl_str_mv Acceso cerrado
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
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dc.rights.creativecommons.none.fl_str_mv 2015-02-05
rights_invalid_str_mv Acceso cerrado
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eu_rights_str_mv closedAccess
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dc.publisher.spa.fl_str_mv Springer
dc.publisher.journal.spa.fl_str_mv Drug Delivery and Translational Research
institution Universidad El Bosque
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spelling Jiménez, Ronald A.Millán, DianaSuesca, EdwardSosnik, AlejandroFontanilla, Marta R.2020-08-06T19:07:54Z2020-08-06T19:07:54Z20152190-3948http://hdl.handle.net/20.500.12495/3714https://doi.org//10.1007/s13346-015-0217-3instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengSpringerDrug Delivery and Translational ResearchDrug Delivery and Translational Research, 2190-3948, Vol. 5, 2015, p. 209–218https://link.springer.com/article/10.1007%2Fs13346-015-0217-3Controlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performanceControlled release of an extract of calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performanceArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Wound healingCollagen I scaffoldsCalendula officinalis LFlowers extractPolyphenolsGelatin-collagenMicroparticle-loaded skin dressingsAiming to develop biological skin dresses with improved performance in the treatment of skin wounds, acellular collagen I scaffolds were modified with polymeric microparticles and the subsequent loading of a hydroglycolic extract of Calendula officinalis flowers. Microparticles made of gelatin-collagen were produced by a water-in-oil emulsion/cross-linking method. Thereafter, these microparticles were mixed with collagen suspensions at three increasing concentrations and the resulting mixtures lyophilized to make microparticle-loaded porous collagen scaffolds. Resistance to enzymatic degradation, ability to associate with the C. officinalis extract, and the extract release profile of the three gelatin-collagen microparticle-scaffold prototypes were assessed in vitro and compared to collagen scaffolds without microparticles used as control. Data indicated that the incorporation of gelatin-collagen microparticles increased the resistance of the scaffolds to in vitro enzymatic degradation, as well as their association with the C. officinalis flower extract. In addition, a sharp decrease in cytotoxicity, as well as more prolonged release of the extract, was attained. Overall results support the potential of these systems to develop innovative dermal substitutes with improved features. Furthermore, the gelatin-collagen mixture represents a low-cost and scalable alternative with high clinical transferability, especially appealing in developing countries.Acceso cerradohttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccess2015-02-05ORIGINALJiménez, Ronald A..pdfJiménez, Ronald A..pdfapplication/pdf3348153http://18.204.144.38/bitstreams/7bc5df84-638e-4e4a-aefc-e83e30dd19d6/downloadf9691baaf1656d5f1215c41c9279d572MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://18.204.144.38/bitstreams/9cb94b1c-d17c-4061-be0c-bdd7c4fa9201/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILMillán, Diana.pdf.jpgMillán, Diana.pdf.jpgimage/jpeg5775http://18.204.144.38/bitstreams/dbe6c672-ba91-4e31-b9d4-c28f4efae4ad/download7210a811635d1799e7c05fee5d259be7MD53Jiménez, Ronald A..pdf.jpgJiménez, Ronald A..pdf.jpgIM Thumbnailimage/jpeg9315http://18.204.144.38/bitstreams/44217cb4-580d-470c-99eb-244f5b4e9971/downloadc3cfce15fa227abe5267eae1bf16e0a8MD54TEXTJiménez, Ronald A..pdf.txtJiménez, Ronald A..pdf.txtExtracted texttext/plain39511http://18.204.144.38/bitstreams/f788a4f9-75fe-46f2-ac65-50e2b8c50703/downloadc311dd32c904be1d35c9a9496252f38fMD5520.500.12495/3714oai:18.204.144.38:20.500.12495/37142024-02-06 22:10:31.531restrictedhttp://18.204.144.38DSpace Pre-instalado Biteca S.A.Sbibliotecas@biteca.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