An epidemiological and molecular study regarding the spread of vancomycinresistant Enterococcus faecium in a teaching hospital in Bogotá, Colombia 2016

Background Enterococcus faecium is ranked worldwide as one of the top ten pathogens identified in healthcare-associated infections (HAI) and is classified as one of the high priority pathogens for research and development of new antibiotics worldwide. Due to molecular biology techniques’ higher costs, the approach for identifying and controlling infectious diseases in developing countries has been based on clinical and epidemiological perspectives. Nevertheless, after an abrupt vancomycin-resistant Enterococcus faecium dissemination in the Méderi teaching hospital, ending up in an outbreak, further measures needed to be taken into consideration. The present study describes the vancomycin-resistant Enterococcus faecium pattern within Colombian’s largest installed-bed capacity hospital in 2016. Methods Thirty-three vancomycin-resistant Enterococcus faecium isolates were recovered during a 5-month period in 2016. Multilocus variable-number tandem-repeat analysis was used for molecular typing to determine clonality amongst strains. A modified time-place-sequence algorithm was used to trace VREfm spread patterns during the outbreak period and estimate transmission routes. Results Four clonal profiles were identified. Chronological clonal profile follow-up suggested a transitional spread from profile “A” to profile “B”, returning to a higher prevalence of “A” by the end of the study. Antibiotic susceptibility indicated high-level vancomycin-resistance in most isolates frequently matching vanA gene identification.

• Autores: Corredor, Nancy Carolina; López, Carolina; Aguilera, Paula Andrea; Rodríguez-Leguizamón, Giovanni; Leal, Aura Lucía; Ovalle-Guerro, María Victoria; Pardo-Oviedo, Juan Mauricio; Patarroyo, Manuel Alfonso; Prieto, Lina María; Chica, Claudia Elena
• Fecha de publicación: 2019
• Institución: Universidad El Bosque
• Repositorio: Repositorio U. El Bosque
• Idioma: eng
• OAI Identifier: oai:repositorio.unbosque.edu.co:20.500.12495/1569
• Acceso en línea: http://hdl.handle.net/20.500.12495/1569; https://doi.org/10.1186/s12879-019-3877-7
• Palabra clave: Enterococcus faecium resistente a la vancomicina; Vigilancia de brotes; Resistencia antimicrobiana; Resistencia a la vancomicina; Brotes de enfermedades; Virología; Vancomycin-resistant Enterococcus faecium; Outbreak surveillance; Antimicrobial resistance
• License: Attribution 4.0 International