Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography

Background Antineoplastic activity has been previously shown for two isomeric flavones, 5,7-dihydroxy-3,6,8-trimethoxy flavone (flavone A) and 3,5-dihydroxy-6,7,8-trimethoxy flavone (flavone B), against colon cancer cell lines (Thomas et al. in PLoS ONE 7:e39806, 5). Here, we present modified methods for the extraction and quantification of flavones A and B in rat colon tissue after intravenous dosing via high performance liquid chromatography, from the originally described procedure for extraction and quantification in rat plasma (Whitted et al. in J Chromatogr B Analyt Technol Biomed Life Sci 1001:150–155, 7). Results Modifications included tissue homogenization (1 g tissue: 2 mL water), filtration of the supernatant with a PVDF membrane, and the use of only one calibration curve to determine the concentration of each flavone in colon tissue. Good separation was achieved and representative equations were linear with r 2 ≥ 0.99 for both flavones. Precision and accuracy for flavone A ranged from 0.88–24.03 and 109–116%. Precision and accuracy for flavone B ranged from 1.62–33.56 and 98–113%. Concentrations of 1639 ± 601 ng/g flavone A and 5975 ± 2480 ng/g of flavone B were detected in rat colon tissue 6 h post dosing. Conclusions Modifications to the extraction methods for flavone A and flavone B from rat colon tissue had good separation, precision, and accuracy.

• Autores: Whitted, Crystal L.; Rodríguez Aguirre, Oscar Eduardo; Palau, Victoria; Torrenegra G., Ruben Dario; Harirforoosh, Sam
• Fecha de publicación: 2017
• Institución: Universidad El Bosque
• Repositorio: Repositorio U. El Bosque
• Idioma: eng
• OAI Identifier: oai:repositorio.unbosque.edu.co:20.500.12495/1575
• Acceso en línea: http://hdl.handle.net/20.500.12495/1575; https://doi.org/10.1186/s13104-016-2358-y
• Palabra clave: Neoplasias; Compuestos heterocíclicos; Flavanonas; 5,7-Dihydroxy-3,6,8-trimethoxy flavone; 3,5-Dihydroxy-6,7,8-trimethoxy flavone; Colon; Flavonoids
• License: Attribution 4.0 International