In vivo infection by a neuroinvasive neurovirulent dengue virus

Although neurological manifestations associated with dengue infections have been reported in endemic countries, the viral or host characteristics determining the infection or alteration of nervous function have not been described. In order to investigate neurobiological conditions related to central...

Full description

Autores:
Velandia-Romero, Myriam Lucía
Acosta-Losada, Orlando
Castellanos, Jaime
Tipo de recurso:
Article of journal
Fecha de publicación:
2012
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/3573
Acceso en línea:
http://hdl.handle.net/20.500.12495/3573
https://doi.org/10.1007/s13365-012-0117-y
https://repositorio.unbosque.edu.co
Palabra clave:
Neurobiología
Neuroblastoma
Oligodendroglía
Dengue virus
Encephalitis
Neuroinfection
Rights
openAccess
License
Acceso abierto
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oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/3573
network_acronym_str UNBOSQUE2
network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.spa.fl_str_mv In vivo infection by a neuroinvasive neurovirulent dengue virus
dc.title.translated.spa.fl_str_mv In vivo infection by a neuroinvasive neurovirulent dengue virus
title In vivo infection by a neuroinvasive neurovirulent dengue virus
spellingShingle In vivo infection by a neuroinvasive neurovirulent dengue virus
Neurobiología
Neuroblastoma
Oligodendroglía
Dengue virus
Encephalitis
Neuroinfection
title_short In vivo infection by a neuroinvasive neurovirulent dengue virus
title_full In vivo infection by a neuroinvasive neurovirulent dengue virus
title_fullStr In vivo infection by a neuroinvasive neurovirulent dengue virus
title_full_unstemmed In vivo infection by a neuroinvasive neurovirulent dengue virus
title_sort In vivo infection by a neuroinvasive neurovirulent dengue virus
dc.creator.fl_str_mv Velandia-Romero, Myriam Lucía
Acosta-Losada, Orlando
Castellanos, Jaime
dc.contributor.author.none.fl_str_mv Velandia-Romero, Myriam Lucía
Acosta-Losada, Orlando
Castellanos, Jaime
dc.contributor.orcid.none.fl_str_mv Castellanos, Jaime [0000-0003-1596-8383]
Velandia-Romero, Myriam Lucía [0000-0002-3340-7304]
dc.subject.decs.spa.fl_str_mv Neurobiología
Neuroblastoma
Oligodendroglía
topic Neurobiología
Neuroblastoma
Oligodendroglía
Dengue virus
Encephalitis
Neuroinfection
dc.subject.keywords.spa.fl_str_mv Dengue virus
Encephalitis
Neuroinfection
description Although neurological manifestations associated with dengue infections have been reported in endemic countries, the viral or host characteristics determining the infection or alteration of nervous function have not been described. In order to investigate neurobiological conditions related to central nervous system dengue virus (DENV) infection, we established a mouse model of neuroinfection. A DENV-4 isolate was first adapted to neuroblastoma cells, later inoculated in suckling mice brain, and finally, this D4MB-6 viral variant was inoculated intraperitoneally in Balb/c mice at different postnatal days (pnd). Virusinduced fatal encephalitis in 2 and 7 pnd mice but infected at 14 and 21 pnd mice survived. The younger mice presented encephalitis at the sixth day postinfection with limb paralysis and postural instability concomitant with efficient viral replication in brain. In this mice model, we found activated microglial cells positive to viral antigen. Neurons, oligodendrocytes, and endothelial cells were also infected by the D4MB-6 virus in neonatal mice, which showed generalized and local plasma leakage with blood-brain barrier (BBB) severe damage. These results suggest that there was a viral fitness change which led to neuroinfection only in immune or neurological immature mice. Infection of neurons, endothelial, and microglial cells may be related to detrimental function or architecture found in susceptible mice. This experimental neuroinfection model could help to have a better understanding of neurological manifestations occurring during severe cases of dengue infection.
publishDate 2012
dc.date.issued.none.fl_str_mv 2012
dc.date.accessioned.none.fl_str_mv 2020-07-23T00:09:22Z
dc.date.available.none.fl_str_mv 2020-07-23T00:09:22Z
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dc.type.local.none.fl_str_mv Artículo de revista
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dc.identifier.issn.none.fl_str_mv 1538-2443
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/3573
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1007/s13365-012-0117-y
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
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identifier_str_mv 1538-2443
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
url http://hdl.handle.net/20.500.12495/3573
https://doi.org/10.1007/s13365-012-0117-y
https://repositorio.unbosque.edu.co
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Journal of neuroVirology, 1538-2443, Vol. 18, Nor. 5, 2012, p. 374-387
dc.relation.uri.none.fl_str_mv https://link.springer.com/article/10.1007/s13365-012-0117-y
dc.rights.local.spa.fl_str_mv Acceso abierto
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
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dc.rights.creativecommons.none.fl_str_mv 2012-07-24
rights_invalid_str_mv Acceso abierto
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2012-07-24
eu_rights_str_mv openAccess
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Springer Nature
dc.publisher.journal.spa.fl_str_mv Journal of neuroVirology
institution Universidad El Bosque
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spelling Velandia-Romero, Myriam LucíaAcosta-Losada, OrlandoCastellanos, JaimeCastellanos, Jaime [0000-0003-1596-8383]Velandia-Romero, Myriam Lucía [0000-0002-3340-7304]2020-07-23T00:09:22Z2020-07-23T00:09:22Z20121538-2443http://hdl.handle.net/20.500.12495/3573https://doi.org/10.1007/s13365-012-0117-yinstname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengSpringer NatureJournal of neuroVirologyJournal of neuroVirology, 1538-2443, Vol. 18, Nor. 5, 2012, p. 374-387https://link.springer.com/article/10.1007/s13365-012-0117-yIn vivo infection by a neuroinvasive neurovirulent dengue virusIn vivo infection by a neuroinvasive neurovirulent dengue virusArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85NeurobiologíaNeuroblastomaOligodendroglíaDengue virusEncephalitisNeuroinfectionAlthough neurological manifestations associated with dengue infections have been reported in endemic countries, the viral or host characteristics determining the infection or alteration of nervous function have not been described. In order to investigate neurobiological conditions related to central nervous system dengue virus (DENV) infection, we established a mouse model of neuroinfection. A DENV-4 isolate was first adapted to neuroblastoma cells, later inoculated in suckling mice brain, and finally, this D4MB-6 viral variant was inoculated intraperitoneally in Balb/c mice at different postnatal days (pnd). Virusinduced fatal encephalitis in 2 and 7 pnd mice but infected at 14 and 21 pnd mice survived. The younger mice presented encephalitis at the sixth day postinfection with limb paralysis and postural instability concomitant with efficient viral replication in brain. In this mice model, we found activated microglial cells positive to viral antigen. Neurons, oligodendrocytes, and endothelial cells were also infected by the D4MB-6 virus in neonatal mice, which showed generalized and local plasma leakage with blood-brain barrier (BBB) severe damage. These results suggest that there was a viral fitness change which led to neuroinfection only in immune or neurological immature mice. Infection of neurons, endothelial, and microglial cells may be related to detrimental function or architecture found in susceptible mice. This experimental neuroinfection model could help to have a better understanding of neurological manifestations occurring during severe cases of dengue infection.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2012-07-24ORIGINALMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdfMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdfapplication/pdf1047882https://repositorio.unbosque.edu.co/bitstreams/b6104ce9-2db4-4af1-b5f8-73f20898b6b3/downloadae16f795a04c5481c3667518955cb661MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/b4cffc9d-005e-439e-b079-f6d5306f79e9/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdf.jpgMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/44a5dffa-92ee-4198-89db-d082aaeb14ca/download7210a811635d1799e7c05fee5d259be7MD53TEXTMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdf.txtMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdf.txtExtracted texttext/plain60158https://repositorio.unbosque.edu.co/bitstreams/5dda8b96-496c-4001-ba53-d406e35852d0/download05d239b2eaf5945282d9e2930bf9b167MD5420.500.12495/3573oai:repositorio.unbosque.edu.co:20.500.12495/35732024-02-07 08:24:23.102restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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