In vivo infection by a neuroinvasive neurovirulent dengue virus
Although neurological manifestations associated with dengue infections have been reported in endemic countries, the viral or host characteristics determining the infection or alteration of nervous function have not been described. In order to investigate neurobiological conditions related to central...
- Autores:
-
Velandia-Romero, Myriam Lucía
Acosta-Losada, Orlando
Castellanos, Jaime
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2012
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/3573
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/3573
https://doi.org/10.1007/s13365-012-0117-y
https://repositorio.unbosque.edu.co
- Palabra clave:
- Neurobiología
Neuroblastoma
Oligodendroglía
Dengue virus
Encephalitis
Neuroinfection
- Rights
- openAccess
- License
- Acceso abierto
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oai:repositorio.unbosque.edu.co:20.500.12495/3573 |
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UNBOSQUE2 |
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Repositorio U. El Bosque |
repository_id_str |
|
dc.title.spa.fl_str_mv |
In vivo infection by a neuroinvasive neurovirulent dengue virus |
dc.title.translated.spa.fl_str_mv |
In vivo infection by a neuroinvasive neurovirulent dengue virus |
title |
In vivo infection by a neuroinvasive neurovirulent dengue virus |
spellingShingle |
In vivo infection by a neuroinvasive neurovirulent dengue virus Neurobiología Neuroblastoma Oligodendroglía Dengue virus Encephalitis Neuroinfection |
title_short |
In vivo infection by a neuroinvasive neurovirulent dengue virus |
title_full |
In vivo infection by a neuroinvasive neurovirulent dengue virus |
title_fullStr |
In vivo infection by a neuroinvasive neurovirulent dengue virus |
title_full_unstemmed |
In vivo infection by a neuroinvasive neurovirulent dengue virus |
title_sort |
In vivo infection by a neuroinvasive neurovirulent dengue virus |
dc.creator.fl_str_mv |
Velandia-Romero, Myriam Lucía Acosta-Losada, Orlando Castellanos, Jaime |
dc.contributor.author.none.fl_str_mv |
Velandia-Romero, Myriam Lucía Acosta-Losada, Orlando Castellanos, Jaime |
dc.contributor.orcid.none.fl_str_mv |
Castellanos, Jaime [0000-0003-1596-8383] Velandia-Romero, Myriam Lucía [0000-0002-3340-7304] |
dc.subject.decs.spa.fl_str_mv |
Neurobiología Neuroblastoma Oligodendroglía |
topic |
Neurobiología Neuroblastoma Oligodendroglía Dengue virus Encephalitis Neuroinfection |
dc.subject.keywords.spa.fl_str_mv |
Dengue virus Encephalitis Neuroinfection |
description |
Although neurological manifestations associated with dengue infections have been reported in endemic countries, the viral or host characteristics determining the infection or alteration of nervous function have not been described. In order to investigate neurobiological conditions related to central nervous system dengue virus (DENV) infection, we established a mouse model of neuroinfection. A DENV-4 isolate was first adapted to neuroblastoma cells, later inoculated in suckling mice brain, and finally, this D4MB-6 viral variant was inoculated intraperitoneally in Balb/c mice at different postnatal days (pnd). Virusinduced fatal encephalitis in 2 and 7 pnd mice but infected at 14 and 21 pnd mice survived. The younger mice presented encephalitis at the sixth day postinfection with limb paralysis and postural instability concomitant with efficient viral replication in brain. In this mice model, we found activated microglial cells positive to viral antigen. Neurons, oligodendrocytes, and endothelial cells were also infected by the D4MB-6 virus in neonatal mice, which showed generalized and local plasma leakage with blood-brain barrier (BBB) severe damage. These results suggest that there was a viral fitness change which led to neuroinfection only in immune or neurological immature mice. Infection of neurons, endothelial, and microglial cells may be related to detrimental function or architecture found in susceptible mice. This experimental neuroinfection model could help to have a better understanding of neurological manifestations occurring during severe cases of dengue infection. |
publishDate |
2012 |
dc.date.issued.none.fl_str_mv |
2012 |
dc.date.accessioned.none.fl_str_mv |
2020-07-23T00:09:22Z |
dc.date.available.none.fl_str_mv |
2020-07-23T00:09:22Z |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.none.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.issn.none.fl_str_mv |
1538-2443 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/3573 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1007/s13365-012-0117-y |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
https://repositorio.unbosque.edu.co |
identifier_str_mv |
1538-2443 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque |
url |
http://hdl.handle.net/20.500.12495/3573 https://doi.org/10.1007/s13365-012-0117-y https://repositorio.unbosque.edu.co |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Journal of neuroVirology, 1538-2443, Vol. 18, Nor. 5, 2012, p. 374-387 |
dc.relation.uri.none.fl_str_mv |
https://link.springer.com/article/10.1007/s13365-012-0117-y |
dc.rights.local.spa.fl_str_mv |
Acceso abierto |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Acceso abierto |
dc.rights.creativecommons.none.fl_str_mv |
2012-07-24 |
rights_invalid_str_mv |
Acceso abierto http://purl.org/coar/access_right/c_abf2 2012-07-24 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Springer Nature |
dc.publisher.journal.spa.fl_str_mv |
Journal of neuroVirology |
institution |
Universidad El Bosque |
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Velandia-Romero, Myriam LucíaAcosta-Losada, OrlandoCastellanos, JaimeCastellanos, Jaime [0000-0003-1596-8383]Velandia-Romero, Myriam Lucía [0000-0002-3340-7304]2020-07-23T00:09:22Z2020-07-23T00:09:22Z20121538-2443http://hdl.handle.net/20.500.12495/3573https://doi.org/10.1007/s13365-012-0117-yinstname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengSpringer NatureJournal of neuroVirologyJournal of neuroVirology, 1538-2443, Vol. 18, Nor. 5, 2012, p. 374-387https://link.springer.com/article/10.1007/s13365-012-0117-yIn vivo infection by a neuroinvasive neurovirulent dengue virusIn vivo infection by a neuroinvasive neurovirulent dengue virusArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85NeurobiologíaNeuroblastomaOligodendroglíaDengue virusEncephalitisNeuroinfectionAlthough neurological manifestations associated with dengue infections have been reported in endemic countries, the viral or host characteristics determining the infection or alteration of nervous function have not been described. In order to investigate neurobiological conditions related to central nervous system dengue virus (DENV) infection, we established a mouse model of neuroinfection. A DENV-4 isolate was first adapted to neuroblastoma cells, later inoculated in suckling mice brain, and finally, this D4MB-6 viral variant was inoculated intraperitoneally in Balb/c mice at different postnatal days (pnd). Virusinduced fatal encephalitis in 2 and 7 pnd mice but infected at 14 and 21 pnd mice survived. The younger mice presented encephalitis at the sixth day postinfection with limb paralysis and postural instability concomitant with efficient viral replication in brain. In this mice model, we found activated microglial cells positive to viral antigen. Neurons, oligodendrocytes, and endothelial cells were also infected by the D4MB-6 virus in neonatal mice, which showed generalized and local plasma leakage with blood-brain barrier (BBB) severe damage. These results suggest that there was a viral fitness change which led to neuroinfection only in immune or neurological immature mice. Infection of neurons, endothelial, and microglial cells may be related to detrimental function or architecture found in susceptible mice. This experimental neuroinfection model could help to have a better understanding of neurological manifestations occurring during severe cases of dengue infection.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2012-07-24ORIGINALMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdfMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdfapplication/pdf1047882https://repositorio.unbosque.edu.co/bitstreams/b6104ce9-2db4-4af1-b5f8-73f20898b6b3/downloadae16f795a04c5481c3667518955cb661MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/b4cffc9d-005e-439e-b079-f6d5306f79e9/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdf.jpgMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/44a5dffa-92ee-4198-89db-d082aaeb14ca/download7210a811635d1799e7c05fee5d259be7MD53TEXTMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdf.txtMyriam Lucia Velandia-Romero ,Orlando Acosta-Losada_2012.pdf.txtExtracted texttext/plain60158https://repositorio.unbosque.edu.co/bitstreams/5dda8b96-496c-4001-ba53-d406e35852d0/download05d239b2eaf5945282d9e2930bf9b167MD5420.500.12495/3573oai:repositorio.unbosque.edu.co:20.500.12495/35732024-02-07 08:24:23.102restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |