In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)

Apicomplexan parasites, including Plasmodium falciparum, are obligate intracellular organisms that utilize a strategy termed “gliding” to move and invade host cells, causing disease. Gliding is carried out by a protein complex known as the glideosome, which includes an actin–myosin motor. To date, s...

Full description

Autores:
Hernández, Paula C.
Wasserman, Moisés
Chaparro-Olaya, Jacqueline
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/3628
Acceso en línea:
http://hdl.handle.net/20.500.12495/3628
https://doi.org/10.1007/s00436-018-6039-8
https://repositorio.unbosque.edu.co
Palabra clave:
Plasmodium falciparum
Myosins
Protein–protein interactions
Pulldown
Far western blot
Rights
openAccess
License
Acceso abierto
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network_acronym_str UNBOSQUE2
network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.spa.fl_str_mv In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)
dc.title.translated.spa.fl_str_mv In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)
title In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)
spellingShingle In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)
Plasmodium falciparum
Myosins
Protein–protein interactions
Pulldown
Far western blot
title_short In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)
title_full In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)
title_fullStr In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)
title_full_unstemmed In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)
title_sort In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)
dc.creator.fl_str_mv Hernández, Paula C.
Wasserman, Moisés
Chaparro-Olaya, Jacqueline
dc.contributor.author.none.fl_str_mv Hernández, Paula C.
Wasserman, Moisés
Chaparro-Olaya, Jacqueline
dc.contributor.orcid.none.fl_str_mv Chaparro-Olaya, Jacqueline [0000-0001-9815-3459]
dc.subject.keywords.spa.fl_str_mv Plasmodium falciparum
Myosins
Protein–protein interactions
Pulldown
Far western blot
topic Plasmodium falciparum
Myosins
Protein–protein interactions
Pulldown
Far western blot
description Apicomplexan parasites, including Plasmodium falciparum, are obligate intracellular organisms that utilize a strategy termed “gliding” to move and invade host cells, causing disease. Gliding is carried out by a protein complex known as the glideosome, which includes an actin–myosin motor. To date, six myosins have been identified in P. falciparum (PfMyoA, B, C, D, E, and F), but only the role of PfMyoA, the myosin of the glideosome that is involved in the process of red blood cell and mosquito cell invasion, has been established. Based on previous observations, we speculated that PfMyoA and PfMyoB may have similar or redundant functions. To test this hypothesis, we searched for in vitro interactions between PfMyoB and MTIP (myosin A tail interacting protein), the myosin light chain of PfMyoA. A set of differentially tagged PfMyoA, PfMyoB, and MTIP recombinant proteins was employed to specifically and simultaneously detect each myosin in competition assays and inhibition assays using specific peptides. MTIP potentially acts as the light chain of PfMyoB.
publishDate 2018
dc.date.issued.none.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2020-07-31T13:14:58Z
dc.date.available.none.fl_str_mv 2020-07-31T13:14:58Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.none.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
format http://purl.org/coar/resource_type/c_6501
dc.identifier.issn.none.fl_str_mv 1432-1955
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/3628
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1007/s00436-018-6039-8
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv https://repositorio.unbosque.edu.co
identifier_str_mv 1432-1955
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
url http://hdl.handle.net/20.500.12495/3628
https://doi.org/10.1007/s00436-018-6039-8
https://repositorio.unbosque.edu.co
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Parasitology Research, 1432-1955, Vol. 117, 2018, p. 3437–3446
dc.relation.uri.none.fl_str_mv https://link.springer.com/article/10.1007/s00436-018-6039-8
dc.rights.local.spa.fl_str_mv Acceso abierto
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
Acceso abierto
dc.rights.creativecommons.none.fl_str_mv 2018-08-09
rights_invalid_str_mv Acceso abierto
http://purl.org/coar/access_right/c_abf2
2018-08-09
eu_rights_str_mv openAccess
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Springer
dc.publisher.journal.spa.fl_str_mv Parasitology Research
institution Universidad El Bosque
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spelling Hernández, Paula C.Wasserman, MoisésChaparro-Olaya, JacquelineChaparro-Olaya, Jacqueline [0000-0001-9815-3459]2020-07-31T13:14:58Z2020-07-31T13:14:58Z20181432-1955http://hdl.handle.net/20.500.12495/3628https://doi.org/10.1007/s00436-018-6039-8instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengSpringerParasitology ResearchParasitology Research, 1432-1955, Vol. 117, 2018, p. 3437–3446https://link.springer.com/article/10.1007/s00436-018-6039-8In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Plasmodium falciparumMyosinsProtein–protein interactionsPulldownFar western blotApicomplexan parasites, including Plasmodium falciparum, are obligate intracellular organisms that utilize a strategy termed “gliding” to move and invade host cells, causing disease. Gliding is carried out by a protein complex known as the glideosome, which includes an actin–myosin motor. To date, six myosins have been identified in P. falciparum (PfMyoA, B, C, D, E, and F), but only the role of PfMyoA, the myosin of the glideosome that is involved in the process of red blood cell and mosquito cell invasion, has been established. Based on previous observations, we speculated that PfMyoA and PfMyoB may have similar or redundant functions. To test this hypothesis, we searched for in vitro interactions between PfMyoB and MTIP (myosin A tail interacting protein), the myosin light chain of PfMyoA. A set of differentially tagged PfMyoA, PfMyoB, and MTIP recombinant proteins was employed to specifically and simultaneously detect each myosin in competition assays and inhibition assays using specific peptides. MTIP potentially acts as the light chain of PfMyoB.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2018-08-09ORIGINALHernández, Paula C..pdfHernández, Paula C..pdfapplication/pdf2940103https://repositorio.unbosque.edu.co/bitstreams/f0609d9a-a152-41b0-85ea-0fedc31de799/downloadccd4fcbcfdd270d5c09b1a3547db2042MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/0616b509-3ab5-4b68-b9cf-ad561ef798db/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILHernández, Paula C..pdf.jpgHernández, Paula C..pdf.jpgIM Thumbnailimage/jpeg9648https://repositorio.unbosque.edu.co/bitstreams/a98a6e9d-3dac-446f-b135-6ad4324801c0/downloadf7a994fdea2e394267c679285e6f7940MD53TEXTHernández, Paula C..pdf.txtHernández, Paula C..pdf.txtExtracted texttext/plain48173https://repositorio.unbosque.edu.co/bitstreams/ea6da749-95f9-4fcd-8e4b-b7413c527bcf/download06ed60866e30b6d58792d3134d4f8c23MD5420.500.12495/3628oai:repositorio.unbosque.edu.co:20.500.12495/36282024-02-07 02:35:57.115restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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