In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)
Apicomplexan parasites, including Plasmodium falciparum, are obligate intracellular organisms that utilize a strategy termed “gliding” to move and invade host cells, causing disease. Gliding is carried out by a protein complex known as the glideosome, which includes an actin–myosin motor. To date, s...
- Autores:
-
Hernández, Paula C.
Wasserman, Moisés
Chaparro-Olaya, Jacqueline
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/3628
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/3628
https://doi.org/10.1007/s00436-018-6039-8
https://repositorio.unbosque.edu.co
- Palabra clave:
- Plasmodium falciparum
Myosins
Protein–protein interactions
Pulldown
Far western blot
- Rights
- openAccess
- License
- Acceso abierto
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Repositorio U. El Bosque |
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|
dc.title.spa.fl_str_mv |
In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP) |
dc.title.translated.spa.fl_str_mv |
In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP) |
title |
In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP) |
spellingShingle |
In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP) Plasmodium falciparum Myosins Protein–protein interactions Pulldown Far western blot |
title_short |
In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP) |
title_full |
In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP) |
title_fullStr |
In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP) |
title_full_unstemmed |
In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP) |
title_sort |
In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP) |
dc.creator.fl_str_mv |
Hernández, Paula C. Wasserman, Moisés Chaparro-Olaya, Jacqueline |
dc.contributor.author.none.fl_str_mv |
Hernández, Paula C. Wasserman, Moisés Chaparro-Olaya, Jacqueline |
dc.contributor.orcid.none.fl_str_mv |
Chaparro-Olaya, Jacqueline [0000-0001-9815-3459] |
dc.subject.keywords.spa.fl_str_mv |
Plasmodium falciparum Myosins Protein–protein interactions Pulldown Far western blot |
topic |
Plasmodium falciparum Myosins Protein–protein interactions Pulldown Far western blot |
description |
Apicomplexan parasites, including Plasmodium falciparum, are obligate intracellular organisms that utilize a strategy termed “gliding” to move and invade host cells, causing disease. Gliding is carried out by a protein complex known as the glideosome, which includes an actin–myosin motor. To date, six myosins have been identified in P. falciparum (PfMyoA, B, C, D, E, and F), but only the role of PfMyoA, the myosin of the glideosome that is involved in the process of red blood cell and mosquito cell invasion, has been established. Based on previous observations, we speculated that PfMyoA and PfMyoB may have similar or redundant functions. To test this hypothesis, we searched for in vitro interactions between PfMyoB and MTIP (myosin A tail interacting protein), the myosin light chain of PfMyoA. A set of differentially tagged PfMyoA, PfMyoB, and MTIP recombinant proteins was employed to specifically and simultaneously detect each myosin in competition assays and inhibition assays using specific peptides. MTIP potentially acts as the light chain of PfMyoB. |
publishDate |
2018 |
dc.date.issued.none.fl_str_mv |
2018 |
dc.date.accessioned.none.fl_str_mv |
2020-07-31T13:14:58Z |
dc.date.available.none.fl_str_mv |
2020-07-31T13:14:58Z |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.none.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.issn.none.fl_str_mv |
1432-1955 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/3628 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1007/s00436-018-6039-8 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
https://repositorio.unbosque.edu.co |
identifier_str_mv |
1432-1955 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque |
url |
http://hdl.handle.net/20.500.12495/3628 https://doi.org/10.1007/s00436-018-6039-8 https://repositorio.unbosque.edu.co |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Parasitology Research, 1432-1955, Vol. 117, 2018, p. 3437–3446 |
dc.relation.uri.none.fl_str_mv |
https://link.springer.com/article/10.1007/s00436-018-6039-8 |
dc.rights.local.spa.fl_str_mv |
Acceso abierto |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Acceso abierto |
dc.rights.creativecommons.none.fl_str_mv |
2018-08-09 |
rights_invalid_str_mv |
Acceso abierto http://purl.org/coar/access_right/c_abf2 2018-08-09 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Springer |
dc.publisher.journal.spa.fl_str_mv |
Parasitology Research |
institution |
Universidad El Bosque |
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spelling |
Hernández, Paula C.Wasserman, MoisésChaparro-Olaya, JacquelineChaparro-Olaya, Jacqueline [0000-0001-9815-3459]2020-07-31T13:14:58Z2020-07-31T13:14:58Z20181432-1955http://hdl.handle.net/20.500.12495/3628https://doi.org/10.1007/s00436-018-6039-8instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengSpringerParasitology ResearchParasitology Research, 1432-1955, Vol. 117, 2018, p. 3437–3446https://link.springer.com/article/10.1007/s00436-018-6039-8In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)In vitro interaction between plasmodium falciparum myosin B (PfMyoB) and myosin a tail interacting protein (MTIP)Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Plasmodium falciparumMyosinsProtein–protein interactionsPulldownFar western blotApicomplexan parasites, including Plasmodium falciparum, are obligate intracellular organisms that utilize a strategy termed “gliding” to move and invade host cells, causing disease. Gliding is carried out by a protein complex known as the glideosome, which includes an actin–myosin motor. To date, six myosins have been identified in P. falciparum (PfMyoA, B, C, D, E, and F), but only the role of PfMyoA, the myosin of the glideosome that is involved in the process of red blood cell and mosquito cell invasion, has been established. Based on previous observations, we speculated that PfMyoA and PfMyoB may have similar or redundant functions. To test this hypothesis, we searched for in vitro interactions between PfMyoB and MTIP (myosin A tail interacting protein), the myosin light chain of PfMyoA. A set of differentially tagged PfMyoA, PfMyoB, and MTIP recombinant proteins was employed to specifically and simultaneously detect each myosin in competition assays and inhibition assays using specific peptides. MTIP potentially acts as the light chain of PfMyoB.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2018-08-09ORIGINALHernández, Paula C..pdfHernández, Paula C..pdfapplication/pdf2940103https://repositorio.unbosque.edu.co/bitstreams/f0609d9a-a152-41b0-85ea-0fedc31de799/downloadccd4fcbcfdd270d5c09b1a3547db2042MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/0616b509-3ab5-4b68-b9cf-ad561ef798db/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILHernández, Paula C..pdf.jpgHernández, Paula C..pdf.jpgIM Thumbnailimage/jpeg9648https://repositorio.unbosque.edu.co/bitstreams/a98a6e9d-3dac-446f-b135-6ad4324801c0/downloadf7a994fdea2e394267c679285e6f7940MD53TEXTHernández, Paula C..pdf.txtHernández, Paula C..pdf.txtExtracted texttext/plain48173https://repositorio.unbosque.edu.co/bitstreams/ea6da749-95f9-4fcd-8e4b-b7413c527bcf/download06ed60866e30b6d58792d3134d4f8c23MD5420.500.12495/3628oai:repositorio.unbosque.edu.co:20.500.12495/36282024-02-07 02:35:57.115restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |