Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America

Background:Vancomycin is a common first-line option for MRSA infections. The heterogeneous vancomycin-intermediateStaphylococcus aureus(hVISA) phenotype is associated with therapeutic failure. However, hVISAisolates are usually reported as vancomycin susceptible by routine susceptibility testing pro...

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Autores:
Castro, Betsy Esperanza
Berrío, Maritza
Vargas, Monica L.
Carvajal Ortiz, Lina Paola
Millan, Lina V.
Rios, Rafael
Hernandez, Angie K.
Rincon Núñez, Sandra
Cubides, Paola
Forero, Erika
Dinh, An Q
Seas, Carlos
Munita, Jose M.
Arias, César A.
Reyes, Jinnethe
Diaz, Lorena
Tipo de recurso:
Article of journal
Fecha de publicación:
2020
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/3909
Acceso en línea:
http://hdl.handle.net/20.500.12495/3909
https://doi.org/10.1093/jac/dkaa221
https://repositorio.unbosque.edu.co
Palabra clave:
Phenotype
Vancomycin
Gait
Heterogeneity
Rights
openAccess
License
Acceso abierto
id UNBOSQUE2_2d05722b3242beebc49301da543b77a8
oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/3909
network_acronym_str UNBOSQUE2
network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.spa.fl_str_mv Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America
dc.title.translated.spa.fl_str_mv Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America
title Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America
spellingShingle Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America
Phenotype
Vancomycin
Gait
Heterogeneity
title_short Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America
title_full Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America
title_fullStr Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America
title_full_unstemmed Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America
title_sort Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America
dc.creator.fl_str_mv Castro, Betsy Esperanza
Berrío, Maritza
Vargas, Monica L.
Carvajal Ortiz, Lina Paola
Millan, Lina V.
Rios, Rafael
Hernandez, Angie K.
Rincon Núñez, Sandra
Cubides, Paola
Forero, Erika
Dinh, An Q
Seas, Carlos
Munita, Jose M.
Arias, César A.
Reyes, Jinnethe
Diaz, Lorena
dc.contributor.author.none.fl_str_mv Castro, Betsy Esperanza
Berrío, Maritza
Vargas, Monica L.
Carvajal Ortiz, Lina Paola
Millan, Lina V.
Rios, Rafael
Hernandez, Angie K.
Rincon Núñez, Sandra
Cubides, Paola
Forero, Erika
Dinh, An Q
Seas, Carlos
Munita, Jose M.
Arias, César A.
Reyes, Jinnethe
Diaz, Lorena
dc.contributor.orcid.none.fl_str_mv Rincon Núñez, Sandra [0000-0002-8482-4554]
Carvajal Ortiz, Lina Paola [0000-0001-8301-8836]
dc.subject.keywords.spa.fl_str_mv Phenotype
Vancomycin
Gait
Heterogeneity
topic Phenotype
Vancomycin
Gait
Heterogeneity
description Background:Vancomycin is a common first-line option for MRSA infections. The heterogeneous vancomycin-intermediateStaphylococcus aureus(hVISA) phenotype is associated with therapeutic failure. However, hVISAisolates are usually reported as vancomycin susceptible by routine susceptibility testing procedures.Objectives:To detect and characterize the hVISA phenotype in MRSA isolates causing infections in nine LatinAmerican countries.Methods:We evaluated a total of 1189 vancomycin-susceptible MRSA isolates recovered during 2006–08 and2011–14. After an initial screening of hVISA using glycopeptide-supplemented agar strategies, the detection ofhVISA was performed by Etest (GRD) and Macro-method (MET). Isolates deemed to be hVISA were subjectedto population analysis profile/AUC (PAP/AUC) and WGS for further characterization. Finally, we interrogatedalterations in predicted proteins associated with the development of the VISA phenotype in both hVISA andvancomycin-susceptibleS. aureus(VSSA) genomes.Results:A total of 39 MRSA isolates (3.3%) were classified as hVISA (1.4% and 5.6% in MRSA recovered from2006–08 and 2011–14, respectively). Most of the hVISA strains (95%) belonged to clonal complex (CC) 5. Only6/39 hVISA isolates were categorized as hVISA by PAP/AUC, with 6 other isolates close (0.87–0.89) to the cut-off(0.9). The majority of the 39 hVISA isolates exhibited the Leu-14!Ile (90%) and VraT Glu-156!Gly (90%) aminoacid substitutions in WalK. Additionally, we identified 10 substitutions present only in hVISA isolates, involvingWalK, VraS, RpoB and RpoC proteins.Conclusions:The hVISA phenotype exhibits low frequency in Latin America. Amino acid substitutions in proteinsinvolved in cell envelope homeostasis and RNA synthesis were commonly identified. Our results suggest thatEtest-based methods are an important alternative for the detection of hVISA clinical isolates.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-09-01T21:21:47Z
dc.date.available.none.fl_str_mv 2020-09-01T21:21:47Z
dc.date.issued.none.fl_str_mv 2020
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.none.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
format http://purl.org/coar/resource_type/c_6501
dc.identifier.issn.none.fl_str_mv 1460-2091
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/3909
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1093/jac/dkaa221
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv https://repositorio.unbosque.edu.co
identifier_str_mv 1460-2091
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
url http://hdl.handle.net/20.500.12495/3909
https://doi.org/10.1093/jac/dkaa221
https://repositorio.unbosque.edu.co
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Journal of antimicrobial chemotherapy, 1460-2091, Vol. 74, Nro. 9, 2020, p. 2424-2431
dc.relation.uri.none.fl_str_mv https://academic.oup.com/jac/article-abstract/75/9/2424/5860393
dc.rights.local.spa.fl_str_mv Acceso abierto
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
Acceso abierto
dc.rights.creativecommons.none.fl_str_mv 2020-04-30
rights_invalid_str_mv Acceso abierto
http://purl.org/coar/access_right/c_abf2
2020-04-30
eu_rights_str_mv openAccess
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Oxford University Press
dc.publisher.journal.spa.fl_str_mv Journal of antimicrobial chemotherapy
institution Universidad El Bosque
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spelling Castro, Betsy EsperanzaBerrío, MaritzaVargas, Monica L.Carvajal Ortiz, Lina PaolaMillan, Lina V.Rios, RafaelHernandez, Angie K.Rincon Núñez, SandraCubides, PaolaForero, ErikaDinh, An QSeas, CarlosMunita, Jose M.Arias, César A.Reyes, JinnetheDiaz, LorenaRincon Núñez, Sandra [0000-0002-8482-4554]Carvajal Ortiz, Lina Paola [0000-0001-8301-8836]2020-09-01T21:21:47Z2020-09-01T21:21:47Z20201460-2091http://hdl.handle.net/20.500.12495/3909https://doi.org/10.1093/jac/dkaa221instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengOxford University PressJournal of antimicrobial chemotherapyJournal of antimicrobial chemotherapy, 1460-2091, Vol. 74, Nro. 9, 2020, p. 2424-2431https://academic.oup.com/jac/article-abstract/75/9/2424/5860393Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin AmericaDetection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin AmericaArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85PhenotypeVancomycinGaitHeterogeneityBackground:Vancomycin is a common first-line option for MRSA infections. The heterogeneous vancomycin-intermediateStaphylococcus aureus(hVISA) phenotype is associated with therapeutic failure. However, hVISAisolates are usually reported as vancomycin susceptible by routine susceptibility testing procedures.Objectives:To detect and characterize the hVISA phenotype in MRSA isolates causing infections in nine LatinAmerican countries.Methods:We evaluated a total of 1189 vancomycin-susceptible MRSA isolates recovered during 2006–08 and2011–14. After an initial screening of hVISA using glycopeptide-supplemented agar strategies, the detection ofhVISA was performed by Etest (GRD) and Macro-method (MET). Isolates deemed to be hVISA were subjectedto population analysis profile/AUC (PAP/AUC) and WGS for further characterization. Finally, we interrogatedalterations in predicted proteins associated with the development of the VISA phenotype in both hVISA andvancomycin-susceptibleS. aureus(VSSA) genomes.Results:A total of 39 MRSA isolates (3.3%) were classified as hVISA (1.4% and 5.6% in MRSA recovered from2006–08 and 2011–14, respectively). Most of the hVISA strains (95%) belonged to clonal complex (CC) 5. Only6/39 hVISA isolates were categorized as hVISA by PAP/AUC, with 6 other isolates close (0.87–0.89) to the cut-off(0.9). The majority of the 39 hVISA isolates exhibited the Leu-14!Ile (90%) and VraT Glu-156!Gly (90%) aminoacid substitutions in WalK. Additionally, we identified 10 substitutions present only in hVISA isolates, involvingWalK, VraS, RpoB and RpoC proteins.Conclusions:The hVISA phenotype exhibits low frequency in Latin America. Amino acid substitutions in proteinsinvolved in cell envelope homeostasis and RNA synthesis were commonly identified. Our results suggest thatEtest-based methods are an important alternative for the detection of hVISA clinical isolates.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2020-04-30THUMBNAILBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdf.jpgBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/aef3602a-3dd2-4ac8-9ef7-2031517d35e2/download7210a811635d1799e7c05fee5d259be7MD53ORIGINALBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdfBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdfapplication/pdf420662https://repositorio.unbosque.edu.co/bitstreams/7f00dbe1-d655-4c87-93d3-5e2bd955503e/downloadf7cd9428dd91166401940b387e43b688MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/7c457088-c07c-4c55-9d9a-171ca030e04c/download8a4605be74aa9ea9d79846c1fba20a33MD52TEXTBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdf.txtBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdf.txtExtracted texttext/plain46479https://repositorio.unbosque.edu.co/bitstreams/600035a6-f40b-41bc-aa6d-254e4f4bf780/download873c5a8f4084d01fa3e94ecfe9fd448eMD5420.500.12495/3909oai:repositorio.unbosque.edu.co:20.500.12495/39092024-02-07 00:18:55.65restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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