Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America
Background:Vancomycin is a common first-line option for MRSA infections. The heterogeneous vancomycin-intermediateStaphylococcus aureus(hVISA) phenotype is associated with therapeutic failure. However, hVISAisolates are usually reported as vancomycin susceptible by routine susceptibility testing pro...
- Autores:
-
Castro, Betsy Esperanza
Berrío, Maritza
Vargas, Monica L.
Carvajal Ortiz, Lina Paola
Millan, Lina V.
Rios, Rafael
Hernandez, Angie K.
Rincon Núñez, Sandra
Cubides, Paola
Forero, Erika
Dinh, An Q
Seas, Carlos
Munita, Jose M.
Arias, César A.
Reyes, Jinnethe
Diaz, Lorena
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/3909
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/3909
https://doi.org/10.1093/jac/dkaa221
https://repositorio.unbosque.edu.co
- Palabra clave:
- Phenotype
Vancomycin
Gait
Heterogeneity
- Rights
- openAccess
- License
- Acceso abierto
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Repositorio U. El Bosque |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America |
dc.title.translated.spa.fl_str_mv |
Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America |
title |
Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America |
spellingShingle |
Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America Phenotype Vancomycin Gait Heterogeneity |
title_short |
Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America |
title_full |
Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America |
title_fullStr |
Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America |
title_full_unstemmed |
Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America |
title_sort |
Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America |
dc.creator.fl_str_mv |
Castro, Betsy Esperanza Berrío, Maritza Vargas, Monica L. Carvajal Ortiz, Lina Paola Millan, Lina V. Rios, Rafael Hernandez, Angie K. Rincon Núñez, Sandra Cubides, Paola Forero, Erika Dinh, An Q Seas, Carlos Munita, Jose M. Arias, César A. Reyes, Jinnethe Diaz, Lorena |
dc.contributor.author.none.fl_str_mv |
Castro, Betsy Esperanza Berrío, Maritza Vargas, Monica L. Carvajal Ortiz, Lina Paola Millan, Lina V. Rios, Rafael Hernandez, Angie K. Rincon Núñez, Sandra Cubides, Paola Forero, Erika Dinh, An Q Seas, Carlos Munita, Jose M. Arias, César A. Reyes, Jinnethe Diaz, Lorena |
dc.contributor.orcid.none.fl_str_mv |
Rincon Núñez, Sandra [0000-0002-8482-4554] Carvajal Ortiz, Lina Paola [0000-0001-8301-8836] |
dc.subject.keywords.spa.fl_str_mv |
Phenotype Vancomycin Gait Heterogeneity |
topic |
Phenotype Vancomycin Gait Heterogeneity |
description |
Background:Vancomycin is a common first-line option for MRSA infections. The heterogeneous vancomycin-intermediateStaphylococcus aureus(hVISA) phenotype is associated with therapeutic failure. However, hVISAisolates are usually reported as vancomycin susceptible by routine susceptibility testing procedures.Objectives:To detect and characterize the hVISA phenotype in MRSA isolates causing infections in nine LatinAmerican countries.Methods:We evaluated a total of 1189 vancomycin-susceptible MRSA isolates recovered during 2006–08 and2011–14. After an initial screening of hVISA using glycopeptide-supplemented agar strategies, the detection ofhVISA was performed by Etest (GRD) and Macro-method (MET). Isolates deemed to be hVISA were subjectedto population analysis profile/AUC (PAP/AUC) and WGS for further characterization. Finally, we interrogatedalterations in predicted proteins associated with the development of the VISA phenotype in both hVISA andvancomycin-susceptibleS. aureus(VSSA) genomes.Results:A total of 39 MRSA isolates (3.3%) were classified as hVISA (1.4% and 5.6% in MRSA recovered from2006–08 and 2011–14, respectively). Most of the hVISA strains (95%) belonged to clonal complex (CC) 5. Only6/39 hVISA isolates were categorized as hVISA by PAP/AUC, with 6 other isolates close (0.87–0.89) to the cut-off(0.9). The majority of the 39 hVISA isolates exhibited the Leu-14!Ile (90%) and VraT Glu-156!Gly (90%) aminoacid substitutions in WalK. Additionally, we identified 10 substitutions present only in hVISA isolates, involvingWalK, VraS, RpoB and RpoC proteins.Conclusions:The hVISA phenotype exhibits low frequency in Latin America. Amino acid substitutions in proteinsinvolved in cell envelope homeostasis and RNA synthesis were commonly identified. Our results suggest thatEtest-based methods are an important alternative for the detection of hVISA clinical isolates. |
publishDate |
2020 |
dc.date.accessioned.none.fl_str_mv |
2020-09-01T21:21:47Z |
dc.date.available.none.fl_str_mv |
2020-09-01T21:21:47Z |
dc.date.issued.none.fl_str_mv |
2020 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.none.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.issn.none.fl_str_mv |
1460-2091 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/3909 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1093/jac/dkaa221 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
https://repositorio.unbosque.edu.co |
identifier_str_mv |
1460-2091 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque |
url |
http://hdl.handle.net/20.500.12495/3909 https://doi.org/10.1093/jac/dkaa221 https://repositorio.unbosque.edu.co |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Journal of antimicrobial chemotherapy, 1460-2091, Vol. 74, Nro. 9, 2020, p. 2424-2431 |
dc.relation.uri.none.fl_str_mv |
https://academic.oup.com/jac/article-abstract/75/9/2424/5860393 |
dc.rights.local.spa.fl_str_mv |
Acceso abierto |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Acceso abierto |
dc.rights.creativecommons.none.fl_str_mv |
2020-04-30 |
rights_invalid_str_mv |
Acceso abierto http://purl.org/coar/access_right/c_abf2 2020-04-30 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Oxford University Press |
dc.publisher.journal.spa.fl_str_mv |
Journal of antimicrobial chemotherapy |
institution |
Universidad El Bosque |
bitstream.url.fl_str_mv |
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spelling |
Castro, Betsy EsperanzaBerrío, MaritzaVargas, Monica L.Carvajal Ortiz, Lina PaolaMillan, Lina V.Rios, RafaelHernandez, Angie K.Rincon Núñez, SandraCubides, PaolaForero, ErikaDinh, An QSeas, CarlosMunita, Jose M.Arias, César A.Reyes, JinnetheDiaz, LorenaRincon Núñez, Sandra [0000-0002-8482-4554]Carvajal Ortiz, Lina Paola [0000-0001-8301-8836]2020-09-01T21:21:47Z2020-09-01T21:21:47Z20201460-2091http://hdl.handle.net/20.500.12495/3909https://doi.org/10.1093/jac/dkaa221instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengOxford University PressJournal of antimicrobial chemotherapyJournal of antimicrobial chemotherapy, 1460-2091, Vol. 74, Nro. 9, 2020, p. 2424-2431https://academic.oup.com/jac/article-abstract/75/9/2424/5860393Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin AmericaDetection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin AmericaArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85PhenotypeVancomycinGaitHeterogeneityBackground:Vancomycin is a common first-line option for MRSA infections. The heterogeneous vancomycin-intermediateStaphylococcus aureus(hVISA) phenotype is associated with therapeutic failure. However, hVISAisolates are usually reported as vancomycin susceptible by routine susceptibility testing procedures.Objectives:To detect and characterize the hVISA phenotype in MRSA isolates causing infections in nine LatinAmerican countries.Methods:We evaluated a total of 1189 vancomycin-susceptible MRSA isolates recovered during 2006–08 and2011–14. After an initial screening of hVISA using glycopeptide-supplemented agar strategies, the detection ofhVISA was performed by Etest (GRD) and Macro-method (MET). Isolates deemed to be hVISA were subjectedto population analysis profile/AUC (PAP/AUC) and WGS for further characterization. Finally, we interrogatedalterations in predicted proteins associated with the development of the VISA phenotype in both hVISA andvancomycin-susceptibleS. aureus(VSSA) genomes.Results:A total of 39 MRSA isolates (3.3%) were classified as hVISA (1.4% and 5.6% in MRSA recovered from2006–08 and 2011–14, respectively). Most of the hVISA strains (95%) belonged to clonal complex (CC) 5. Only6/39 hVISA isolates were categorized as hVISA by PAP/AUC, with 6 other isolates close (0.87–0.89) to the cut-off(0.9). The majority of the 39 hVISA isolates exhibited the Leu-14!Ile (90%) and VraT Glu-156!Gly (90%) aminoacid substitutions in WalK. Additionally, we identified 10 substitutions present only in hVISA isolates, involvingWalK, VraS, RpoB and RpoC proteins.Conclusions:The hVISA phenotype exhibits low frequency in Latin America. Amino acid substitutions in proteinsinvolved in cell envelope homeostasis and RNA synthesis were commonly identified. Our results suggest thatEtest-based methods are an important alternative for the detection of hVISA clinical isolates.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2020-04-30THUMBNAILBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdf.jpgBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/aef3602a-3dd2-4ac8-9ef7-2031517d35e2/download7210a811635d1799e7c05fee5d259be7MD53ORIGINALBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdfBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdfapplication/pdf420662https://repositorio.unbosque.edu.co/bitstreams/7f00dbe1-d655-4c87-93d3-5e2bd955503e/downloadf7cd9428dd91166401940b387e43b688MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/7c457088-c07c-4c55-9d9a-171ca030e04c/download8a4605be74aa9ea9d79846c1fba20a33MD52TEXTBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdf.txtBetsy E Castro, Maritza Berrio, Monica L Vargas_2020.pdf.txtExtracted texttext/plain46479https://repositorio.unbosque.edu.co/bitstreams/600035a6-f40b-41bc-aa6d-254e4f4bf780/download873c5a8f4084d01fa3e94ecfe9fd448eMD5420.500.12495/3909oai:repositorio.unbosque.edu.co:20.500.12495/39092024-02-07 00:18:55.65restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |