First report and comparative genomics analysis of a blaoxa-244-haarboring escherichia coli isolate recovered in the American continent

The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study, and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harboring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38, and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbor resistance genes. The blaOXA-244 gene was chromosomally encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. To our knowledge, this is the first report of a blaOXA-244–harboring Escherichia coli isolate in America. Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently in America.

• Autores: Abril Riaño, Deisy Julieth; Bustos Moya, Ingrid Gisell; Márquez-Ortiz, Ricaurte Alejandro; Josa, Diego; Corredor Rozo, Zayda Lorena; Torres Molina, Isabel; Vanegas Gómez, Natasha; Escobar-Pérez, Javier
• Fecha de publicación: 2019
• Institución: Universidad El Bosque
• Repositorio: Repositorio U. El Bosque
• Idioma: eng
• OAI Identifier: oai:repositorio.unbosque.edu.co:20.500.12495/2088
• Acceso en línea: http://hdl.handle.net/20.500.12495/2088; https://doi.org/10.3390/antibiotics8040222
• Palabra clave: Código genético; Informes de casos; Enterobacteriaceae; Carbapenems; Resistance; BlaOXA-244
• Rights: openAccess
• License: Attribution 4.0 International