Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica
Las infecciones intra-amnióticas son una de las principales causas que desencadenan eventos adversos en el embarazo y pueden estar asociadas a infecciones crónicas como la enfermedad periodontal. Se han descrito dos mecanismos asociados con la degradación de la decidua; uno por una vía directa a tra...
- Autores:
-
Gomez Peña, Luz Amparo
- Tipo de recurso:
- Fecha de publicación:
- 2017
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/10100
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/10100
- Palabra clave:
- Parto pre-término
Infecciones intra-amnióticas
Células NK
Linfocitos T colaboradores
Macrófagos
Preterm birth
Intra-amniotic infection
Th lymphocyte
Macrophage
Porphyromonas gingivalis
WU 100
- Rights
- closedAccess
- License
- Acceso cerrado
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| dc.title.spa.fl_str_mv |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| dc.title.translated.spa.fl_str_mv |
Cytokine profiles in placental tissue of women with adverse pregnancy outcomes associated with intra-amniotic infection |
| title |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| spellingShingle |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica Parto pre-término Infecciones intra-amnióticas Células NK Linfocitos T colaboradores Macrófagos Preterm birth Intra-amniotic infection Th lymphocyte Macrophage Porphyromonas gingivalis WU 100 |
| title_short |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| title_full |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| title_fullStr |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| title_full_unstemmed |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| title_sort |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| dc.creator.fl_str_mv |
Gomez Peña, Luz Amparo |
| dc.contributor.advisor.none.fl_str_mv |
Lafaurie Villamil, Gloria Ines de Avila Quiroga, Juliette |
| dc.contributor.author.none.fl_str_mv |
Gomez Peña, Luz Amparo |
| dc.subject.spa.fl_str_mv |
Parto pre-término Infecciones intra-amnióticas Células NK Linfocitos T colaboradores Macrófagos |
| topic |
Parto pre-término Infecciones intra-amnióticas Células NK Linfocitos T colaboradores Macrófagos Preterm birth Intra-amniotic infection Th lymphocyte Macrophage Porphyromonas gingivalis WU 100 |
| dc.subject.keywords.spa.fl_str_mv |
Preterm birth Intra-amniotic infection Th lymphocyte Macrophage Porphyromonas gingivalis |
| dc.subject.nlm.none.fl_str_mv |
WU 100 |
| description |
Las infecciones intra-amnióticas son una de las principales causas que desencadenan eventos adversos en el embarazo y pueden estar asociadas a infecciones crónicas como la enfermedad periodontal. Se han descrito dos mecanismos asociados con la degradación de la decidua; uno por una vía directa a través de los factores de virulencia de los microorganismos y otro indirecto asociado a la activación de citocinas en respuesta a antígenos presentes en la decidua. Establecer la asociación de la expresión de citocinas representativas de LTh1, LTh17, célula NK, macrófago y los perfiles microbianos aislados de infecciones intra-amnióticas con la presencia de BPN, RPM, PPT y/o signos clínicos de infección intra-amniótica. Se realizó un estudio observacional de corte transversal, en donde se examinaron un total de 210 mujeres durante el parto. Se seleccionaron 40 mujeres embarazadas, que se dividieron en cinco grupos:1) mujeres que no presentaron ningún resultado de evento adverso o infección intra-amniótica (n = 17), 2) mujeres sin infección intra-amniótica pero con presencia de algún evento adverso (n = 9), 3) mujeres con infección intra-amniótica por P. gingivalis y algún resultado de evento adverso (n = 5), 4) mujeres con infección intra-amniótica por P. gingivalis sin resultado de evento adverso (n = 4) y 5) mujeres con infección intra-amniótica relacionadas con microorganismos de importancia médica (n = 5). A todas las pacientes se les realizó un examen clínico obstétrico, toma de muestras de placenta y sangre de cordon umbilical con un diagnóstico periodontal en el momento del parto. Se determinó el perfil microbiológico por PCR anidada para microorganismos de origen oral y uro-genital en tejido placentario y la concentración de citocinas asociadas a perfiles de LT-ayudador por citometría de flujo. Los métodos estadísticos incluyeron análisis bivariado por Chi2, análisis de componentes principales entre los patrones de respuesta de las citocinas durante el parto, Kruskal-Wallis para la comparación entre mediana de concentraciones de citocinas y ANOVA de una via post-hoc Dummet para comparar la relación de citocinas/ IFNγ; entre los grupos. El 60% de las mujeres en el grupo de infección intra-amniótica por P. gingivalis tenían periodontitis según criterios CDC, y también P. gingivalis en la placa subgingival (p <0,05). Todos los grupos mostraron un perfil Th1 predominante que se caracterizó por la sobreexpresión de IFNγ y su asociación con la activación de la célula Natural Killer por IL-15 y Granzima B (p <0,005). Se observó una reducción significativa de las citocinas IL-10 e IL17F entre los grupos, mostrando reducciones significativas en el grupo de P. gingivalis con presencia de evento adverso (p <0,005). Además, este grupo mostró algunas variaciones sobre el patrón Th1, que exhiben una influencia significativa de las citocinas de macrófagos MCP-1, TNFα, IL-21 (p <0.05). El grupo control no mostró ninguna diferencia con infección intra-amniótica relacionada a microorganismos de importancia médica. La infección placentaria con P. gingivalis en mujeres embarazadas altera significativamente las concentraciones de citocinas que están directamente relacionadas con el embarazo. La presencia de esta bacteria puede activar directamente al MCP-1 sin ser inducido por altas concentraciones de citocinas del perfil Th1 como el IFNγ; posiblemente por exposición a patrones moleculares derivados del patógeno por vía hematógena generando eventos adversos en el embarazo. |
| publishDate |
2017 |
| dc.date.issued.none.fl_str_mv |
2017 |
| dc.date.accessioned.none.fl_str_mv |
2023-03-03T18:32:32Z |
| dc.date.available.none.fl_str_mv |
2023-03-03T18:32:32Z |
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http://purl.org/coar/resource_type/c_7a1f |
| dc.type.local.spa.fl_str_mv |
Tesis/Trabajo de grado - Monografía - Maestría |
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info:eu-repo/semantics/acceptedVersion |
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info:eu-repo/semantics/bachelorThesis |
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acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/10100 |
| dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
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reponame:Repositorio Institucional Universidad El Bosque |
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repourl:https://repositorio.unbosque.edu.co |
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http://hdl.handle.net/20.500.12495/10100 |
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instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque repourl:https://repositorio.unbosque.edu.co |
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spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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(2009) The roles of CCR6 in migration of Th-17 cells and regulation of effector T-cell balance in the gut. Mucosal Immunology. 2(2); 11. Williams CE, Davenport ES, Sterne JA, Sivapathasundaram V, Fearne JM, Curtis MA. Mechanisms of risk in preterm low-birthweight infants. Periodontol 2000. 2000 Jun;23:142-50. Wimmer G, Pihlstrom BL. A critical assessment of adverse pregnancy outcome and periodontal disease. J Clin Periodontol. 2008 Sep;35(8 Suppl):380-97. WHO. International statistical classification of diseases and related health problems. Instruction manual. Volume 2. 10th revision, edition 2010. Geneva, Switzerland. Retrieved from http://books.google.com/books?hl=es&lr=&id=Tw5eAtsatiUC&oi=fnd&pg=PA1&dq=International+Statistical+Classification+of+Diseases+and+Related+Health+Problems&ots=o1d4e2pJlK&sig=1eu2vtjopxMalKI9qgAFd7qRDBc WHO. Maternal Mortality in 2005. Estimates developed by WHO, UNICEF, UNFPA and The World Bank. Ginebra, 2007. URL disponible en: http://www.who.int/reproductivehealth/publications/maternal_mortality_2005/ Xu M, Yamada M. Li M, Liu H, Chen SG, Han YW. FadA from Fusobacterium nucleatum utilizes both secreted and nonsecreted forms for funtional oligomerization for attachment and invasion of host cells. J Biol Chem. 2007;282(34):25000-9. Xiong X, Buekens P, Vastardis S, Yu SM. Periodontal disease and pregnancy outcomes: stateof-the-science. Obstet Gynecol Surv. 2007;62(9):605-15. Xiong X, Buekens P, Fraser WD, Beck J, Offenbacher S. Periodontal disease and adverse pregnancy outcomes: a systematic review. BJOG. 2006 Feb;113(2):135-43. Zaga-Clavellina V, Flores-Espinosa P, Pineda-Torres M, Sosa-González I, Vega-Sánchez R, Estrada-Gutierrez G, et al. Tissue-specific IL-10 secretion profile from term human fetal membranes stimulated with pathogenic microorganisms associated with preterm labor in a two- compartment tissue culture system. J Matern Fetal Neonatal Med. 2014;27(13):1320-7. |
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Lafaurie Villamil, Gloria Inesde Avila Quiroga, JulietteGomez Peña, Luz Amparo2023-03-03T18:32:32Z2023-03-03T18:32:32Z2017http://hdl.handle.net/20.500.12495/10100instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coLas infecciones intra-amnióticas son una de las principales causas que desencadenan eventos adversos en el embarazo y pueden estar asociadas a infecciones crónicas como la enfermedad periodontal. Se han descrito dos mecanismos asociados con la degradación de la decidua; uno por una vía directa a través de los factores de virulencia de los microorganismos y otro indirecto asociado a la activación de citocinas en respuesta a antígenos presentes en la decidua. Establecer la asociación de la expresión de citocinas representativas de LTh1, LTh17, célula NK, macrófago y los perfiles microbianos aislados de infecciones intra-amnióticas con la presencia de BPN, RPM, PPT y/o signos clínicos de infección intra-amniótica. Se realizó un estudio observacional de corte transversal, en donde se examinaron un total de 210 mujeres durante el parto. Se seleccionaron 40 mujeres embarazadas, que se dividieron en cinco grupos:1) mujeres que no presentaron ningún resultado de evento adverso o infección intra-amniótica (n = 17), 2) mujeres sin infección intra-amniótica pero con presencia de algún evento adverso (n = 9), 3) mujeres con infección intra-amniótica por P. gingivalis y algún resultado de evento adverso (n = 5), 4) mujeres con infección intra-amniótica por P. gingivalis sin resultado de evento adverso (n = 4) y 5) mujeres con infección intra-amniótica relacionadas con microorganismos de importancia médica (n = 5). A todas las pacientes se les realizó un examen clínico obstétrico, toma de muestras de placenta y sangre de cordon umbilical con un diagnóstico periodontal en el momento del parto. Se determinó el perfil microbiológico por PCR anidada para microorganismos de origen oral y uro-genital en tejido placentario y la concentración de citocinas asociadas a perfiles de LT-ayudador por citometría de flujo. Los métodos estadísticos incluyeron análisis bivariado por Chi2, análisis de componentes principales entre los patrones de respuesta de las citocinas durante el parto, Kruskal-Wallis para la comparación entre mediana de concentraciones de citocinas y ANOVA de una via post-hoc Dummet para comparar la relación de citocinas/ IFNγ; entre los grupos. El 60% de las mujeres en el grupo de infección intra-amniótica por P. gingivalis tenían periodontitis según criterios CDC, y también P. gingivalis en la placa subgingival (p <0,05). Todos los grupos mostraron un perfil Th1 predominante que se caracterizó por la sobreexpresión de IFNγ y su asociación con la activación de la célula Natural Killer por IL-15 y Granzima B (p <0,005). Se observó una reducción significativa de las citocinas IL-10 e IL17F entre los grupos, mostrando reducciones significativas en el grupo de P. gingivalis con presencia de evento adverso (p <0,005). Además, este grupo mostró algunas variaciones sobre el patrón Th1, que exhiben una influencia significativa de las citocinas de macrófagos MCP-1, TNFα, IL-21 (p <0.05). El grupo control no mostró ninguna diferencia con infección intra-amniótica relacionada a microorganismos de importancia médica. La infección placentaria con P. gingivalis en mujeres embarazadas altera significativamente las concentraciones de citocinas que están directamente relacionadas con el embarazo. La presencia de esta bacteria puede activar directamente al MCP-1 sin ser inducido por altas concentraciones de citocinas del perfil Th1 como el IFNγ; posiblemente por exposición a patrones moleculares derivados del patógeno por vía hematógena generando eventos adversos en el embarazo.Magíster en Ciencias OdontológicasMaestríaThe intra-amniotic infections are main causes of adverse outcomes on the pregnancy, and some of these infections can be related with periodontal disease. It has been described two ways associated with the decidua damage. One of this correspond with a direct effect of microorganisms or its virulence factors; the other way refers to activation of mechanisms of response against decidual bacterial antigens and it is recognized as an indirect way. The aim of this study was to establish the association between the expression of cytokines patterns from LTh1, LTh7, NK cell and macrophage cell lines and the placental microbiota profiles with adverse outcomes as LBW, PMR, PTB and other clinical signals of intraamniotic infection. It was performed a cross sectional study where it was evaluated 210 pregnant women during delivery. Forty pregnant women were included in this study, which were divided in five groups: 1) women which didn’t have any adverse outcome or intraamniotic infection (n=17); 2) women which without intraamniotic infection but had some adverse outcome (n=9); 3) women with P. gingivalis intraamniotic infection and some adverse outcome (n=5); 4) women with P. gingivalis intra-amniotic infection without adverse outcome (n=4) and 5) women with intra-amniotic infection related with medical relevance microorganisms (n=5). Which received a obstetric and periodontal evaluation and It was taken samples of placenta and umbilical blood, also subgingival plaque in the moment of them deliveries. It was determined the microbiological profile in placenta and gingival plaque using nested PCR. All included women were distributed in five groups according the microbiota profile and adverse outcome condition and it was measured the levels of IL1β, IL6, TNF-α, MCP-1α, IL15, Granzyme B, IL17A, IL17F, IL21, IL21, IL12p70 and IFN-γ, in placental samples using a multiplex flow cytometry assay. The statistical analysis did include a bivariate evaluation by chi square test, a principal components group by group test, and comparisons by Kruskal Wallis test, U Mann Whitney test or one way ANOVA with Dunnett correction. All analysis were made using IBM-SPSS V23 for Windows and R software. 60% of women in P. gingivalis intraamniotic group had periodontitis by CDC criteria, which also had P. gingivalis in periodontal plaque (p<0.05). All patients showed a predominant Th1 profile which it was characterized by the IFNγ over-expression and its association with NK linage activation by IL-15 and Granzyme B (p <0.005). It was observed a significant reduction of IL-10 and IL17F cytokines among groups (p<0.05), showing significant reductions in P. gingivalis group (p< 0,005). Also, P. gingivalis group showed some variations about the Th1 pattern, which exhibit a significant influence of the macrophage cytokines MCP1, TNFα, IL-21 (p<0.05). Control group did not show any difference with intra-amniotic infection related with medical relevance microorganisms group. The evidence has been showing the relationship between periodontitis with adverse outcomes of pregnancy, however this is the first study that shows how P. gingivalis intraamniotic infection can induce a significant reduction of regulatory cytokines as well as increased levels of MPC-1 a cytokine associated with macrophage activation, explaining its possible relationship with adverse outcomes in pregnancy.application/pdfspaParto pre-términoInfecciones intra-amnióticasCélulas NKLinfocitos T colaboradoresMacrófagosPreterm birthIntra-amniotic infectionTh lymphocyteMacrophagePorphyromonas gingivalisWU 100Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amnióticaCytokine profiles in placental tissue of women with adverse pregnancy outcomes associated with intra-amniotic infectionMaestría en Ciencias OdontológicasUniversidad El BosqueFacultad de OdontologíaTesis/Trabajo de grado - Monografía - Maestríainfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisAbati S, Villa A, Cetin I, Dessole S, Lugliè PF, Strohmenger L, Ottolenghi L, Campus GG. 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