Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica
Las infecciones intra-amnióticas son una de las principales causas que desencadenan eventos adversos en el embarazo y pueden estar asociadas a infecciones crónicas como la enfermedad periodontal. Se han descrito dos mecanismos asociados con la degradación de la decidua; uno por una vía directa a tra...
- Autores:
-
Gomez Peña, Luz Amparo
- Tipo de recurso:
- Fecha de publicación:
- 2017
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/10100
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/10100
- Palabra clave:
- Parto pre-término
Infecciones intra-amnióticas
Células NK
Linfocitos T colaboradores
Macrófagos
Preterm birth
Intra-amniotic infection
Th lymphocyte
Macrophage
Porphyromonas gingivalis
WU 100
- Rights
- closedAccess
- License
- Acceso cerrado
| id |
UNBOSQUE2_23452052c8b794ac257bc64055267edb |
|---|---|
| oai_identifier_str |
oai:repositorio.unbosque.edu.co:20.500.12495/10100 |
| network_acronym_str |
UNBOSQUE2 |
| network_name_str |
Repositorio U. El Bosque |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| dc.title.translated.spa.fl_str_mv |
Cytokine profiles in placental tissue of women with adverse pregnancy outcomes associated with intra-amniotic infection |
| title |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| spellingShingle |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica Parto pre-término Infecciones intra-amnióticas Células NK Linfocitos T colaboradores Macrófagos Preterm birth Intra-amniotic infection Th lymphocyte Macrophage Porphyromonas gingivalis WU 100 |
| title_short |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| title_full |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| title_fullStr |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| title_full_unstemmed |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| title_sort |
Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica |
| dc.creator.fl_str_mv |
Gomez Peña, Luz Amparo |
| dc.contributor.advisor.none.fl_str_mv |
Lafaurie Villamil, Gloria Ines de Avila Quiroga, Juliette |
| dc.contributor.author.none.fl_str_mv |
Gomez Peña, Luz Amparo |
| dc.subject.spa.fl_str_mv |
Parto pre-término Infecciones intra-amnióticas Células NK Linfocitos T colaboradores Macrófagos |
| topic |
Parto pre-término Infecciones intra-amnióticas Células NK Linfocitos T colaboradores Macrófagos Preterm birth Intra-amniotic infection Th lymphocyte Macrophage Porphyromonas gingivalis WU 100 |
| dc.subject.keywords.spa.fl_str_mv |
Preterm birth Intra-amniotic infection Th lymphocyte Macrophage Porphyromonas gingivalis |
| dc.subject.nlm.none.fl_str_mv |
WU 100 |
| description |
Las infecciones intra-amnióticas son una de las principales causas que desencadenan eventos adversos en el embarazo y pueden estar asociadas a infecciones crónicas como la enfermedad periodontal. Se han descrito dos mecanismos asociados con la degradación de la decidua; uno por una vía directa a través de los factores de virulencia de los microorganismos y otro indirecto asociado a la activación de citocinas en respuesta a antígenos presentes en la decidua. Establecer la asociación de la expresión de citocinas representativas de LTh1, LTh17, célula NK, macrófago y los perfiles microbianos aislados de infecciones intra-amnióticas con la presencia de BPN, RPM, PPT y/o signos clínicos de infección intra-amniótica. Se realizó un estudio observacional de corte transversal, en donde se examinaron un total de 210 mujeres durante el parto. Se seleccionaron 40 mujeres embarazadas, que se dividieron en cinco grupos:1) mujeres que no presentaron ningún resultado de evento adverso o infección intra-amniótica (n = 17), 2) mujeres sin infección intra-amniótica pero con presencia de algún evento adverso (n = 9), 3) mujeres con infección intra-amniótica por P. gingivalis y algún resultado de evento adverso (n = 5), 4) mujeres con infección intra-amniótica por P. gingivalis sin resultado de evento adverso (n = 4) y 5) mujeres con infección intra-amniótica relacionadas con microorganismos de importancia médica (n = 5). A todas las pacientes se les realizó un examen clínico obstétrico, toma de muestras de placenta y sangre de cordon umbilical con un diagnóstico periodontal en el momento del parto. Se determinó el perfil microbiológico por PCR anidada para microorganismos de origen oral y uro-genital en tejido placentario y la concentración de citocinas asociadas a perfiles de LT-ayudador por citometría de flujo. Los métodos estadísticos incluyeron análisis bivariado por Chi2, análisis de componentes principales entre los patrones de respuesta de las citocinas durante el parto, Kruskal-Wallis para la comparación entre mediana de concentraciones de citocinas y ANOVA de una via post-hoc Dummet para comparar la relación de citocinas/ IFNγ; entre los grupos. El 60% de las mujeres en el grupo de infección intra-amniótica por P. gingivalis tenían periodontitis según criterios CDC, y también P. gingivalis en la placa subgingival (p <0,05). Todos los grupos mostraron un perfil Th1 predominante que se caracterizó por la sobreexpresión de IFNγ y su asociación con la activación de la célula Natural Killer por IL-15 y Granzima B (p <0,005). Se observó una reducción significativa de las citocinas IL-10 e IL17F entre los grupos, mostrando reducciones significativas en el grupo de P. gingivalis con presencia de evento adverso (p <0,005). Además, este grupo mostró algunas variaciones sobre el patrón Th1, que exhiben una influencia significativa de las citocinas de macrófagos MCP-1, TNFα, IL-21 (p <0.05). El grupo control no mostró ninguna diferencia con infección intra-amniótica relacionada a microorganismos de importancia médica. La infección placentaria con P. gingivalis en mujeres embarazadas altera significativamente las concentraciones de citocinas que están directamente relacionadas con el embarazo. La presencia de esta bacteria puede activar directamente al MCP-1 sin ser inducido por altas concentraciones de citocinas del perfil Th1 como el IFNγ; posiblemente por exposición a patrones moleculares derivados del patógeno por vía hematógena generando eventos adversos en el embarazo. |
| publishDate |
2017 |
| dc.date.issued.none.fl_str_mv |
2017 |
| dc.date.accessioned.none.fl_str_mv |
2023-03-03T18:32:32Z |
| dc.date.available.none.fl_str_mv |
2023-03-03T18:32:32Z |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_7a1f |
| dc.type.local.spa.fl_str_mv |
Tesis/Trabajo de grado - Monografía - Maestría |
| dc.type.hasversion.none.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/10100 |
| dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
| dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
| dc.identifier.repourl.none.fl_str_mv |
repourl:https://repositorio.unbosque.edu.co |
| url |
http://hdl.handle.net/20.500.12495/10100 |
| identifier_str_mv |
instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque repourl:https://repositorio.unbosque.edu.co |
| dc.language.iso.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
Abati S, Villa A, Cetin I, Dessole S, Lugliè PF, Strohmenger L, Ottolenghi L, Campus GG. Lack of association between maternal periodontal status and adverse pregnancy outcomes: a multicentric epidemiologic study. J Matern Fetal Neonatal Med. 2013;26(4):369-72. Abrahams V, Potter J, Bhat G, Peltier M, Saade G, Menon R. Bacterial modulation of human fetal membrane toll-like receptor expression. Am J Reprod Immunol. 2012;69(1):33-40. ACOG Committee on Practice Bulletins-Obstetrics. ACOG Practice Bulletin No. 80: premature rupture of membranes. Clinical management guidelines for obstetrician-gynecologists. Obstet Gynecol. 2007;109(4):1007–19. Africa C. Oral colonization of Gram-negative anaerobes as a risk factor for preterm delivery. Virulence. 2011;2(6):498-8. Alan T, Tita N, William W. Andrews. Diagnosis and Management of Clinical Chorioamnionitis. Clin Perinatol. 2010; 37(2): 339-54. Alijotas-Reig J, Llurba E, Gris J. Potentiating maternal immune tolerance in pregnancy: A new challenging role for regulatory T cells. Placenta. 2014;35(4):241-8. American Academy of Periodontology task force report on the update to the 1999 classification of periodontal diseases and conditions. J Periodontol. 2015;86(7):835-8. Epub 2015 May 27. Arenas-Hernandez M, Romero R, St Louis D, Hassan S, Kaye E, Gomez-Lopez N. An imbalance between innate and adaptive immune cells at the maternal–fetal interface occurs prior to endotoxin-induced preterm birth. Cell and Mol Immunol. 2015;13(4):462-73. Armitage GC. Bi-directional relationship between pregnancy and periodontal disease. Periodontol. 2000. 2013; 61(1):160–76. Amsel R, Totten PA, Spiegel CA, Chen KCS, Eschenbach DA, Holmes KK. Nonspecific vaginitis: diagnostic criteria and microbial and epidemiologic associations. Am J Med. 1983;74(1):14–22. Apantaku O, Mulik V. Maternal intra-partum fever. J Obstet Gynaecol. 2007; 27(1):12–5. Ashimoto A, Chen C, Bakker I, Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral microbiol and immunol. 1996;11(4):266-73. Baccaglini L. A meta-analysis of randomized controlled trials shows no evidence that periodontal treatment during pregnancy prevents adverse pregnancy outcomes. J Am Dent Assoc 2011;142(10):1192-3. Barak S, Oettinger-Barak O, Machtei, EE, Sprecher H, Ohel G. Evidence of periopathogenic microorganisms in placentas of women with preeclampsia. J periodontol. 2007;78(4),670–6. Bardou M, Hadi T, Mace G, Pesant M, Debermont J, Barrichon M et al. Systemic increase in human maternal circulating CD14+CD16− MCP-1+ monocytes as a marker of labor. Am J Obstet Gynecol. 2014;210(1):70.e1-9. Baskaradoss JK, Geevarghese A, Kutty VR. Maternal periodontal status and preterm delivery: a hospital based case–control study. J Periodontal Res. 2011;46(5):542–9. Baskaradoss JK, Geevarghese A, Al Dosari AA. Causes of Adverse Pregnancy Outcomes and the Role of Maternal Periodontal Status–A Review of the Literature. Open Dent J. 2012;6:79-84. Beck S, Wojdyla D, Say L, Betran AP, Merialdi M, Requejo JH, Rubens C, Menon R, Van Look PF.The worldwide incidence of preterm birth: a systematic review of maternal mortality and morbidity. Bull World Health Organ. 2010;88(1):31-8. Epub 2009 Sep 25. BioLegend’s LEGENDplex™. www.biolegend.com/legendplex, 2016. Blanc V, O'Valle F, Pozo E, Puertas A, León R, Mesa F. Oral bacteria in placental tissues: increased molecular detection in pregnant periodontitis patients. Oral Dis. 2015;21(7):905-12. Blencowe H, Cousens S, Oestergaard MZ, Chou D, Moller AB, Narwal R, et al. National, regional, and worldwide estimates of preterm birth rates in the year 2010 with time trends since 1990 for selected countries: a systematic analysis and implications. Lancet. 2012 (9);379(9832):2162-72. Bird M, Karavitis J, Kovacs E. Sex differences and estrogen modulation of the cellular immune response after injury. Cell Immunol. 2008;252(1-2):57-67. Cao B, Stout M, Lee I, Mysorekar I. Placental Microbiome and Its Role in Preterm Birth. NeoReviews. 2014;15(12):e537-e545. Castillo DM, Sánchez-Beltrán MC, Castellanos JE, Sanz I, Mayorga-Fayad I, Sanz M et al. Detection of specific periodontal microorganisms from bacteraemia samples after periodontal therapy using molecular-based diagnostics. J Clin Periodontol. 2011;38(5):418-27. Chaim W, Mazor M. Intraamniotic infection with fusobacteria. Arch Gynecol Obstet. 1992;251(1):1-7. Chambrone L, Pannuti CM, Guglielmetti MR, Chambrone LA. Evidence grade associating periodontitis with preterm birth and/or low birth weight: II: A systematic review of randomized trials evaluating the effects of periodontal treatment. J Clin Periodontol. 2011;38: 902–14. Cobo T, Kacerovsky M, Holst R, Hougaard D, Skogstrand K, Wennerholm U, et al. Intra-amniotic lammation predicts microbial invasion of the amniotic cavity but not spontaneous preterm delivery in preterm prelabor membrane rupture. Acta Obstet Gynecol Scand. 2012;91(8):930-5. Darmochwal-Kolarz D, Kludka-Sternik M, Tabarkiewicz J, Kolarz B, Rolinski J, LeszczynskaGorzelak B, et al. The predominance of Th17 lymphocytes and decreased number and function of Treg cells in preeclampsia; J Reprod Immunol. 2012;93(2):75-81. Department of Reproductive Health and Research (RHR), WHO - UNICEF. Low birth weight - Country, regional and global estimates. 2005. URL disponible en: http://www.who.int/reproductive-health/publications/low_birthweight/index.html Dixon NG, Ebright D, Defrancesco MA, Hawkins RE. Orogenital contact: a cause of chorioamnionitis. Obstet Gynecol. 1994;84(4 Pt 2):654-5. Ercan E, Eratalay K, Deren O, Gur D, Ozyuncu O, Altun B et al. Evaluation of periodontal pathogens in amniotic fluid and the role of periodontal disease in pre-term birth and low birth weight. Acta Odontol Scand. 2013;71(3-4):553-9 Erlebacher A. Immunology of the maternal-fetal interface. Annu Rev Immunol. 2013;31:387-11. Ernest JM, Wasilauskas B. Capnocytophaga in the amniotic fluid of a woman in preterm labor with intact membranes. Am J Obstet Gynecol. 1985;153(6):648-9. Esplin M, Romero R, Chaiworapongsa T, Kim Y, Edwin S, Gomez R et al. Monocyte chemotactic protein-1 is increased in the amniotic fluid of women who deliver preterm in the presence or absence of intra-amniotic infection. J Matern Fetal Neonatal Med. 2005;17(6):36573. Ferraz EM, Gray RH, Cunha TM. Determinants of preterm delivery and intrauterine growth retardation in north-east Brazil. Int J Epidemiol. 1990; 19(1):101-8. Galinsky R, Polglase GR, Hooper SB, Black MJ, Moss TJ. The Consequences of Chorioamnionitis: Preterm Birth and Effects on Development, J Pregnancy. 2013;2013:412831. Epub 2013 Mar 7. Garcia-Ruíz G, Flores-Espinosa P, Preciado-Martínez E, Bermejo-Martínez L, Espejel-Nuñez A, Estrada-Gutierrez G, et al. In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1β, TNFα, IL-6, IL-8, IL-10, MIP-1α, and MMP-9 in pre-labor human term placenta. Reprod Biol Endocrinol. 2015;13: 115. Gauthier S, Tétu A, Himaya E, Morand M, Chandad F, Rallu F et al. The origin of Fusobacterium nucleatum involved in intra-amniotic infection and preterm birth. J Matern Fetal Neonatal Med. 2011;24(11):1329-32. Gibbs RS, Duff P. Progress in pathogenesis and management of clinical intraamniotic infection. Am J Obstet Gynecol. 1991;164(5 Pt 1):1317-26. Goldenberg RL, Culhane JF. Infection as a cause of preterm birth. Clin Perinatol. 2003;30(4):677-700. Goldenberg RL, Culhane JF, Iams JD, Romero R. Epidemiology and causes of preterm birth. Lancet. 2008 Jan 5;371(9606):75-84. Gomes-Filho IS, Cruz SS, Rezende EJ, Dos Santos CA, Soledade KR, Magalhães MA, et al. Exposure measurement in the association between periodontal disease and prematurity/low birth weight. J Clin Periodontol. 2007;34(11):957-63. Gonzales-Marin C, Spratt DA, Millar MR, Simmonds M, Kempley ST, Allaker RP. Levels of periodontal pathogens in neonatal gastric aspirates and possible maternal sites of origin. Mol Oral Microbiol. 2011;26(5):277-90. Haffajee AD, Socransky SS, Patel MR, Song X. Microbial complexes in supragingival plaque. Oral Microbiol Immunol. 2008 Jun;23(3):196-205. Han YW, Shen T, Chung P, Buhimschi IA, Buhimschi CS. Uncultivated Bacteria as Etiologic Agents of Intra-Amniotic Inflammation Leading to Preterm Birth. J Clin Microbiol. 2009 Jan;47(1):38-47. Han YW, Fardini Y, Chen C, Iacampo KJ, Peraino VA, Shamonki JM, et al. Term stillbirth caused by oral Fusobacterium nucleatum. Obstet Gynecol 2010;115(2 Pt 2):442-5. Han, Y. W. Oral Health and Adverse Pregnancy Outcomes–What’s Next? J Dent Res. 2011;90(3):289-93. Hanna N, Bonifacio L, Weinberger B, Reddy P, Murphy S, Romero R et al. Evidence for Interleukin-10-Mediated Inhibition of Cyclo- oxygenase-2 Expression and Prostaglandin Production in Preterm Human Placenta. Am J Reprod Immunol. 2006;55(1):19-27 Hasegawa-Nakamura K, Tateishi F, Nakamura T, Nakajima Y, Kawamata K, Douchi T, Hatae M, Noguchi K. (2011). The possible mechanism of preterm birth associated with periodontopathic Porphyromonas gingivalis. J Periodont Res 2011; 46: 497–504. Harmon QE, Engel SM, Olshan AF, Moran T, Stuebe AM, Luo J, et al. Association of Polymorphisms in Natural Killer Cell-Related Genes With Preterm Birth. Am J Epidemiol. 2013 Oct 15;178(8):1208-18. Hillier SL, Nugent RP, Eschenbach DA, Krohn MA, Gibbs RS, Martin DH, et al. Association between Bacterial Vaginosis and Preterm Delivery of a Low-Birth-Weight Infant. N Engl J Med. 1995;333(26):1737-42. Ide M, Papapanou PN. Epidemiology of association between maternal periodontal disease and adverse pregnancy outcomes – systematic review. J Periodontol. 2013;84(4 Suppl):S181-94. Inaba H, Kuboniwa M, Sugita H, Lamont R, Amano A. Identification of Signaling Pathways Mediating Cell Cycle Arrest and Apoptosis Induced by Porphyromonas gingivalis in Human Trophoblasts. Infect Immun. 2012;80(8):2847-57. Ito M, Nakashima A, Hidaka T, Okabe M, Bac N, Ina S et al. A role for IL-17 in induction of an inflammation at the fetomaternal interface in preterm labour. J Reprod Immunol. 2010;84(1):7585. Jacobsson B, Holst R, Wennerholm U, Andersson B, Lilja H, Hagberg H. Monocyte chemotactic protein-1 in cervical and amniotic fluid: relationship to microbial invasion of the amniotic cavity, intra-amniotic inflammation, and preterm delivery. Am J Obstet Gynecol. 2003;189(4):1161-7. Katz J, Chegini N, Shiverick K, Lamont R. Localization of P. gingivalis in Preterm Delivery Placenta. Jour J Dent Res. 2009;88(6):575-8. Kraus T, Engel S, Sperling R, Kellerman L, Lo Y, Wallenstein S et al. Characterizing the Pregnancy Immune Phenotype: Results of the Viral Immunity and Pregnancy (VIP) Study. J Clin Immunol. 2012;32(2):300-11. Lafaurie GI, Contreras A, Barón A, Botero J, Mayorga-Fayad I, Jaramillo A, et al. Demographic, clinical, and microbial aspects of chronic and aggressive periodontitis in Colombia: a multicenter study. J Periodontol. 2007;78(4):629-39. León R, Silva N, Ovalle A, Chaparro A, Ahumada A, Gajardo M et al. Detection of Porphyromonas gingivalis in the Amniotic Fluid in Pregnant Women With a Diagnosis of Threatened Premature Labor. J Periodontol. 2007;78(7):1249-55. Lin D, Moss K, Beck JD, Hefti, A, Offenbacher S. Persistently high levels of periodontal pathogens associated with preterm pregnancy outcome. J Periodontol. 2007;78(5):833-41. Madianos PN, Lieff S, Murtha AP, Boggess KA, Auten RL Jr, Beck JD, Offenbacher S. Maternal periodontitis and prematurity. Part II: Maternal infection and fetal exposure. Ann Periodontol. 2001;6(1):175-82. Madianos, P. N., Bobetsis, G. A., & Kinane, D. F. (2002). Is periodontitis associated with an increased risk of coronary heart disease and preterm and/or low birth weight births? Journal of Clinical Periodontology, 29(s3), 22–36. doi:10.1034/j.1600-051X.29.s3.2. Madianos PN, Bobetsis YA, Offenbacher S. Adverse pregnancy outcomes (APOs) and periodontal disease: pathogenic mechanisms. J Periodontol. 2013;84(4 Suppl):S170-80. Mahmoudi Rad M, Zafarghandi ASh, Amel Zabihi M, Tavallaee M, Mirdamadi Y. Identification of Candida species associated with vulvovaginal candidiasis by multiplex PCR. Infect Dis Obstet Gynecol. 2012;2012:872169. Marin C, Segura-Egea JJ, Martínez-Sahuquillo A, Bullón P. Correlation between infant birth weight and mother’s periodontal status. J Clin Periodontol. 2005;32(3):299-304. McCormick MC. The contribution of low birth weight to ant mortality and chilhood morbidity. N Engl J Med. 1985 (10);312(2):82-90. Mendz GL, Kaakoush NO, Quinlivan JA. Bacterial aetiological agents of intra-amniotic infections and preterm birth in pregnant women. Front Cell Infect Microbiol. 2013;3:58. Michalowicz BS, Durand R. Maternal periodontal disease and spontaneous preterm birth. Periodontol 2000. 2007;44:103-12. Michalowicz BS, Gustafsson A, Thumbigere-Math V, Buhlin K.The effects of periodontal treatment on pregnancy outcomes. J Periodontol. 2013 Apr;84(4 Suppl):S195-208. Mobini M, Mortazavi M, Nadi S, Zare-Bidaki M, Pourtalebi S, Arababadi MK. Significant roles played by interleukin-10 in outcome of pregnancy. Iran J Basic Med Sci. 2016;19(2):119-24. Moliterno LF, Monteiro B, Figueredo CM, Fischer RG. Association between periodontitis and low birth weight: a case–control study. J Clin Periodontol. 2005;32(8):886-90. Moreli JB, Corrêa-Silva S, Damasceno D, Sinzato Y, Lorenzon-Ojea A, Borbely Au, et al. Changes in the TNF-alpha/IL-10 ratio in hyperglycemia-associated pregnancies. Diabetes Res Clin Pract. 2015;107(3):362-9. Moore ML, Michielutte R, Meiss PJ, Ernest JM, Wells HB, Buescher PA. Etiology of low-birth weight birth: a population-based study. Prev Med 1994;23(6):793-9. Newton ER. Chorioamnionitis and intraamniotic infection. Clin Obstet Gynecol. 1993;36(4):795808. Noack M, Miossec P. Th17 and regulatory T cell balance Autoimmun Rev. 2014;13(6):668-77. Offenbacher S, Jared HL, O'Reilly PG, Wells SR, Salvi GE, Lawrence HP, et al. Potential pathogenic mechanisms of periodontitis associated pregnancy complications. Ann Periodontol. 1998;3(1):233-50. Pineda-Torres M, Flores-Espinosa P, Espejel-Nunez A, Estrada-Gutierrez G, Flores-Pliego A, Maida-Claros R et al. Evidence of an immunosuppressive effect of progesterone uponin vitrosecretion of proinflammatory and prodegradative factors in a model of choriodecidual infection. BJOG. 2015;122(13):1798-807. Prince A, Ma J, Kannan P, Alvarez M, Gisslen T, Harris R, et al. The placental membrane microbiome is altered among subjects with spontaneous preterm birth with and without chorioamnionitis. Am J Obstet Gynecol. 2016;214(5):627.e1-627.e16. Profamilia. Encuesta Nacional de Demografía y Salud ENDS 2005. Capítulo10. http://www.profamilia.org.co/encuestas/Profamilia/Profamilia/images/stories/ENDS2 05/general/capitulo_X.pdf Profamilia. Encuesta Nacional de Demografía y Salud ENDS 2010. Capítulo10. http://www.profamilia.org.co/encuestas/Profamilia/Profamilia/images/stories/PDFcapitulos/Capitulo-10.pdf Riewe S, Mans J, Hirano T, Katz J, Shiverick K, Brown T, et al. Human trophoblast responses to Porphyromonas gingivalis infection. Mol Oral Microbiol. 2010;25(4):252-9. Romero R, Chaemsaithong P, Docheva N, Korzeniewski S, Tarca A, Bhatti G, et al. Clinical chorioamnionitis at term V: umbilical cord plasma cytokine profile in the context of a systemic maternal inflammatory response. J Perinat Med. 2016;44(1):53-76. Saito S, Nakashima A, Shima T, Ito M. Th1 ⁄ Th2 ⁄ Th17 and regulatory T-cell paradigm in pregnancy. Am J Reprod Immunol. 2010;63(6):601-10. Santos-Pereira SA, Giraldo PC, Saba-Chujfi E, Amaral RL, Morais SS, Fachini AM, et al. Chronic periodontitis and pre-term labour in Brazilian pregnant women: an association to be analysed. J Clin Periodontol. 2007;34(3):208-13. Santa Cruz I, Herrera D, Martin C, Herrero A, Sanz M. Association between periodontal status and pre-term and/or low-birth weight in Spain: clinical and microbiological parameters. J Periodontal Res. 2013;48(4):443-51. Schatz F, Guzeloglu-Kayisli O, Arlier S, Kayisli U, Lockwood C. The role of decidual cells in uterine hemostasis, menstruation, inflammation, adverse pregnancy outcomes and abnormal uterine bleeding. Hum Reprod Update. 2016;22(4):497-515. Szaryńska M, Myśliwski A, Myśliwska J, Kmieć Z, Preis K, Zabul P. Cytokine profiles during delivery affect cord blood hematopoietic stem and progenitors cells. Cell Immunol. 2015;293(2):137-41. Toldi G, Rigó J, Stenczer B, Vásárhelyi B, Molvarec A. Increased Prevalence of IL-17-Producing Peripheral Blood Lymphocytes in Pre-eclampsia. Am J Reprod Immunol. 2011;66(3):223-9. Vacca P, Mingari M, Moretta L. Natural killer cells in human pregnancy. J Reprod Immunol. 2013;97(1):14-9. Vanterpool S, Been J, Houben M, Nikkels P, De Krijger R, Zimmermann L, et al. Porphyromonas gingivalis within Placental Villous Mesenchyme and Umbilical Cord Stroma Is Associated with Adverse Pregnancy Outcome. PLoS One. 2016;11(1):e0146157. Vettore MV1, Leão AT, Leal Mdo C, Feres M, Sheiham A. The relationship between periodontal disease and preterm low birthweight: clinical and microbiological results. J Periodontal Res. 2008;43(6):615-26. Wang C, SG Kang, J Lee, Z Sun and CH Kim. (2009) The roles of CCR6 in migration of Th-17 cells and regulation of effector T-cell balance in the gut. Mucosal Immunology. 2(2); 11. Williams CE, Davenport ES, Sterne JA, Sivapathasundaram V, Fearne JM, Curtis MA. Mechanisms of risk in preterm low-birthweight infants. Periodontol 2000. 2000 Jun;23:142-50. Wimmer G, Pihlstrom BL. A critical assessment of adverse pregnancy outcome and periodontal disease. J Clin Periodontol. 2008 Sep;35(8 Suppl):380-97. WHO. International statistical classification of diseases and related health problems. Instruction manual. Volume 2. 10th revision, edition 2010. Geneva, Switzerland. Retrieved from http://books.google.com/books?hl=es&lr=&id=Tw5eAtsatiUC&oi=fnd&pg=PA1&dq=International+Statistical+Classification+of+Diseases+and+Related+Health+Problems&ots=o1d4e2pJlK&sig=1eu2vtjopxMalKI9qgAFd7qRDBc WHO. Maternal Mortality in 2005. Estimates developed by WHO, UNICEF, UNFPA and The World Bank. Ginebra, 2007. URL disponible en: http://www.who.int/reproductivehealth/publications/maternal_mortality_2005/ Xu M, Yamada M. Li M, Liu H, Chen SG, Han YW. FadA from Fusobacterium nucleatum utilizes both secreted and nonsecreted forms for funtional oligomerization for attachment and invasion of host cells. J Biol Chem. 2007;282(34):25000-9. Xiong X, Buekens P, Vastardis S, Yu SM. Periodontal disease and pregnancy outcomes: stateof-the-science. Obstet Gynecol Surv. 2007;62(9):605-15. Xiong X, Buekens P, Fraser WD, Beck J, Offenbacher S. Periodontal disease and adverse pregnancy outcomes: a systematic review. BJOG. 2006 Feb;113(2):135-43. Zaga-Clavellina V, Flores-Espinosa P, Pineda-Torres M, Sosa-González I, Vega-Sánchez R, Estrada-Gutierrez G, et al. Tissue-specific IL-10 secretion profile from term human fetal membranes stimulated with pathogenic microorganisms associated with preterm labor in a two- compartment tissue culture system. J Matern Fetal Neonatal Med. 2014;27(13):1320-7. |
| dc.rights.local.spa.fl_str_mv |
Acceso cerrado |
| dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/closedAccess http://purl.org/coar/access_right/c_14cb |
| rights_invalid_str_mv |
Acceso cerrado http://purl.org/coar/access_right/c_14cb |
| eu_rights_str_mv |
closedAccess |
| dc.format.mimetype.none.fl_str_mv |
application/pdf |
| dc.publisher.program.spa.fl_str_mv |
Maestría en Ciencias Odontológicas |
| dc.publisher.grantor.spa.fl_str_mv |
Universidad El Bosque |
| dc.publisher.faculty.spa.fl_str_mv |
Facultad de Odontología |
| institution |
Universidad El Bosque |
| bitstream.url.fl_str_mv |
https://repositorio.unbosque.edu.co/bitstreams/803ed7d9-6925-4717-a3a2-c633ef789916/download https://repositorio.unbosque.edu.co/bitstreams/a8f70042-82ec-4b44-883c-52edf9a56654/download https://repositorio.unbosque.edu.co/bitstreams/56e9b91e-ddc0-4a55-a625-88a185f94d58/download https://repositorio.unbosque.edu.co/bitstreams/33504c69-a46d-4ac9-9cd1-cfed4831a720/download https://repositorio.unbosque.edu.co/bitstreams/7cbe7c14-b8e0-4e28-b3fe-6495dca41e54/download https://repositorio.unbosque.edu.co/bitstreams/c737c057-8c5b-4dc9-a9fc-01a67293a859/download |
| bitstream.checksum.fl_str_mv |
1a2b58c99c09896e23844ebbcba6a499 8a4605be74aa9ea9d79846c1fba20a33 53958a7c941c7288a7c33bc4e59de4f2 81a174a936f9bf341e21160656df8778 7366b787fbf4ae2b5be61643220e4b05 8411dc60a2bca4415fb398c476b70c35 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional Universidad El Bosque |
| repository.mail.fl_str_mv |
bibliotecas@biteca.com |
| _version_ |
1814100695294410752 |
| spelling |
Lafaurie Villamil, Gloria Inesde Avila Quiroga, JulietteGomez Peña, Luz Amparo2023-03-03T18:32:32Z2023-03-03T18:32:32Z2017http://hdl.handle.net/20.500.12495/10100instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coLas infecciones intra-amnióticas son una de las principales causas que desencadenan eventos adversos en el embarazo y pueden estar asociadas a infecciones crónicas como la enfermedad periodontal. Se han descrito dos mecanismos asociados con la degradación de la decidua; uno por una vía directa a través de los factores de virulencia de los microorganismos y otro indirecto asociado a la activación de citocinas en respuesta a antígenos presentes en la decidua. Establecer la asociación de la expresión de citocinas representativas de LTh1, LTh17, célula NK, macrófago y los perfiles microbianos aislados de infecciones intra-amnióticas con la presencia de BPN, RPM, PPT y/o signos clínicos de infección intra-amniótica. Se realizó un estudio observacional de corte transversal, en donde se examinaron un total de 210 mujeres durante el parto. Se seleccionaron 40 mujeres embarazadas, que se dividieron en cinco grupos:1) mujeres que no presentaron ningún resultado de evento adverso o infección intra-amniótica (n = 17), 2) mujeres sin infección intra-amniótica pero con presencia de algún evento adverso (n = 9), 3) mujeres con infección intra-amniótica por P. gingivalis y algún resultado de evento adverso (n = 5), 4) mujeres con infección intra-amniótica por P. gingivalis sin resultado de evento adverso (n = 4) y 5) mujeres con infección intra-amniótica relacionadas con microorganismos de importancia médica (n = 5). A todas las pacientes se les realizó un examen clínico obstétrico, toma de muestras de placenta y sangre de cordon umbilical con un diagnóstico periodontal en el momento del parto. Se determinó el perfil microbiológico por PCR anidada para microorganismos de origen oral y uro-genital en tejido placentario y la concentración de citocinas asociadas a perfiles de LT-ayudador por citometría de flujo. Los métodos estadísticos incluyeron análisis bivariado por Chi2, análisis de componentes principales entre los patrones de respuesta de las citocinas durante el parto, Kruskal-Wallis para la comparación entre mediana de concentraciones de citocinas y ANOVA de una via post-hoc Dummet para comparar la relación de citocinas/ IFNγ; entre los grupos. El 60% de las mujeres en el grupo de infección intra-amniótica por P. gingivalis tenían periodontitis según criterios CDC, y también P. gingivalis en la placa subgingival (p <0,05). Todos los grupos mostraron un perfil Th1 predominante que se caracterizó por la sobreexpresión de IFNγ y su asociación con la activación de la célula Natural Killer por IL-15 y Granzima B (p <0,005). Se observó una reducción significativa de las citocinas IL-10 e IL17F entre los grupos, mostrando reducciones significativas en el grupo de P. gingivalis con presencia de evento adverso (p <0,005). Además, este grupo mostró algunas variaciones sobre el patrón Th1, que exhiben una influencia significativa de las citocinas de macrófagos MCP-1, TNFα, IL-21 (p <0.05). El grupo control no mostró ninguna diferencia con infección intra-amniótica relacionada a microorganismos de importancia médica. La infección placentaria con P. gingivalis en mujeres embarazadas altera significativamente las concentraciones de citocinas que están directamente relacionadas con el embarazo. La presencia de esta bacteria puede activar directamente al MCP-1 sin ser inducido por altas concentraciones de citocinas del perfil Th1 como el IFNγ; posiblemente por exposición a patrones moleculares derivados del patógeno por vía hematógena generando eventos adversos en el embarazo.Magíster en Ciencias OdontológicasMaestríaThe intra-amniotic infections are main causes of adverse outcomes on the pregnancy, and some of these infections can be related with periodontal disease. It has been described two ways associated with the decidua damage. One of this correspond with a direct effect of microorganisms or its virulence factors; the other way refers to activation of mechanisms of response against decidual bacterial antigens and it is recognized as an indirect way. The aim of this study was to establish the association between the expression of cytokines patterns from LTh1, LTh7, NK cell and macrophage cell lines and the placental microbiota profiles with adverse outcomes as LBW, PMR, PTB and other clinical signals of intraamniotic infection. It was performed a cross sectional study where it was evaluated 210 pregnant women during delivery. Forty pregnant women were included in this study, which were divided in five groups: 1) women which didn’t have any adverse outcome or intraamniotic infection (n=17); 2) women which without intraamniotic infection but had some adverse outcome (n=9); 3) women with P. gingivalis intraamniotic infection and some adverse outcome (n=5); 4) women with P. gingivalis intra-amniotic infection without adverse outcome (n=4) and 5) women with intra-amniotic infection related with medical relevance microorganisms (n=5). Which received a obstetric and periodontal evaluation and It was taken samples of placenta and umbilical blood, also subgingival plaque in the moment of them deliveries. It was determined the microbiological profile in placenta and gingival plaque using nested PCR. All included women were distributed in five groups according the microbiota profile and adverse outcome condition and it was measured the levels of IL1β, IL6, TNF-α, MCP-1α, IL15, Granzyme B, IL17A, IL17F, IL21, IL21, IL12p70 and IFN-γ, in placental samples using a multiplex flow cytometry assay. The statistical analysis did include a bivariate evaluation by chi square test, a principal components group by group test, and comparisons by Kruskal Wallis test, U Mann Whitney test or one way ANOVA with Dunnett correction. All analysis were made using IBM-SPSS V23 for Windows and R software. 60% of women in P. gingivalis intraamniotic group had periodontitis by CDC criteria, which also had P. gingivalis in periodontal plaque (p<0.05). All patients showed a predominant Th1 profile which it was characterized by the IFNγ over-expression and its association with NK linage activation by IL-15 and Granzyme B (p <0.005). It was observed a significant reduction of IL-10 and IL17F cytokines among groups (p<0.05), showing significant reductions in P. gingivalis group (p< 0,005). Also, P. gingivalis group showed some variations about the Th1 pattern, which exhibit a significant influence of the macrophage cytokines MCP1, TNFα, IL-21 (p<0.05). Control group did not show any difference with intra-amniotic infection related with medical relevance microorganisms group. The evidence has been showing the relationship between periodontitis with adverse outcomes of pregnancy, however this is the first study that shows how P. gingivalis intraamniotic infection can induce a significant reduction of regulatory cytokines as well as increased levels of MPC-1 a cytokine associated with macrophage activation, explaining its possible relationship with adverse outcomes in pregnancy.application/pdfspaParto pre-términoInfecciones intra-amnióticasCélulas NKLinfocitos T colaboradoresMacrófagosPreterm birthIntra-amniotic infectionTh lymphocyteMacrophagePorphyromonas gingivalisWU 100Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amnióticaCytokine profiles in placental tissue of women with adverse pregnancy outcomes associated with intra-amniotic infectionMaestría en Ciencias OdontológicasUniversidad El BosqueFacultad de OdontologíaTesis/Trabajo de grado - Monografía - Maestríainfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisAbati S, Villa A, Cetin I, Dessole S, Lugliè PF, Strohmenger L, Ottolenghi L, Campus GG. Lack of association between maternal periodontal status and adverse pregnancy outcomes: a multicentric epidemiologic study. J Matern Fetal Neonatal Med. 2013;26(4):369-72.Abrahams V, Potter J, Bhat G, Peltier M, Saade G, Menon R. Bacterial modulation of human fetal membrane toll-like receptor expression. Am J Reprod Immunol. 2012;69(1):33-40.ACOG Committee on Practice Bulletins-Obstetrics. ACOG Practice Bulletin No. 80: premature rupture of membranes. Clinical management guidelines for obstetrician-gynecologists. Obstet Gynecol. 2007;109(4):1007–19.Africa C. Oral colonization of Gram-negative anaerobes as a risk factor for preterm delivery. Virulence. 2011;2(6):498-8.Alan T, Tita N, William W. Andrews. Diagnosis and Management of Clinical Chorioamnionitis. Clin Perinatol. 2010; 37(2): 339-54.Alijotas-Reig J, Llurba E, Gris J. Potentiating maternal immune tolerance in pregnancy: A new challenging role for regulatory T cells. Placenta. 2014;35(4):241-8.American Academy of Periodontology task force report on the update to the 1999 classification of periodontal diseases and conditions. J Periodontol. 2015;86(7):835-8. Epub 2015 May 27.Arenas-Hernandez M, Romero R, St Louis D, Hassan S, Kaye E, Gomez-Lopez N. An imbalance between innate and adaptive immune cells at the maternal–fetal interface occurs prior to endotoxin-induced preterm birth. Cell and Mol Immunol. 2015;13(4):462-73.Armitage GC. Bi-directional relationship between pregnancy and periodontal disease. Periodontol. 2000. 2013; 61(1):160–76.Amsel R, Totten PA, Spiegel CA, Chen KCS, Eschenbach DA, Holmes KK. Nonspecific vaginitis: diagnostic criteria and microbial and epidemiologic associations. Am J Med. 1983;74(1):14–22.Apantaku O, Mulik V. Maternal intra-partum fever. J Obstet Gynaecol. 2007; 27(1):12–5.Ashimoto A, Chen C, Bakker I, Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral microbiol and immunol. 1996;11(4):266-73.Baccaglini L. A meta-analysis of randomized controlled trials shows no evidence that periodontal treatment during pregnancy prevents adverse pregnancy outcomes. J Am Dent Assoc 2011;142(10):1192-3.Barak S, Oettinger-Barak O, Machtei, EE, Sprecher H, Ohel G. Evidence of periopathogenic microorganisms in placentas of women with preeclampsia. J periodontol. 2007;78(4),670–6.Bardou M, Hadi T, Mace G, Pesant M, Debermont J, Barrichon M et al. Systemic increase in human maternal circulating CD14+CD16− MCP-1+ monocytes as a marker of labor. Am J Obstet Gynecol. 2014;210(1):70.e1-9.Baskaradoss JK, Geevarghese A, Kutty VR. Maternal periodontal status and preterm delivery: a hospital based case–control study. J Periodontal Res. 2011;46(5):542–9.Baskaradoss JK, Geevarghese A, Al Dosari AA. Causes of Adverse Pregnancy Outcomes and the Role of Maternal Periodontal Status–A Review of the Literature. Open Dent J. 2012;6:79-84.Beck S, Wojdyla D, Say L, Betran AP, Merialdi M, Requejo JH, Rubens C, Menon R, Van Look PF.The worldwide incidence of preterm birth: a systematic review of maternal mortality and morbidity. Bull World Health Organ. 2010;88(1):31-8. Epub 2009 Sep 25.BioLegend’s LEGENDplex™. www.biolegend.com/legendplex, 2016.Blanc V, O'Valle F, Pozo E, Puertas A, León R, Mesa F. Oral bacteria in placental tissues: increased molecular detection in pregnant periodontitis patients. Oral Dis. 2015;21(7):905-12.Blencowe H, Cousens S, Oestergaard MZ, Chou D, Moller AB, Narwal R, et al. National, regional, and worldwide estimates of preterm birth rates in the year 2010 with time trends since 1990 for selected countries: a systematic analysis and implications. Lancet. 2012 (9);379(9832):2162-72.Bird M, Karavitis J, Kovacs E. Sex differences and estrogen modulation of the cellular immune response after injury. Cell Immunol. 2008;252(1-2):57-67.Cao B, Stout M, Lee I, Mysorekar I. Placental Microbiome and Its Role in Preterm Birth. NeoReviews. 2014;15(12):e537-e545.Castillo DM, Sánchez-Beltrán MC, Castellanos JE, Sanz I, Mayorga-Fayad I, Sanz M et al. Detection of specific periodontal microorganisms from bacteraemia samples after periodontal therapy using molecular-based diagnostics. J Clin Periodontol. 2011;38(5):418-27.Chaim W, Mazor M. Intraamniotic infection with fusobacteria. Arch Gynecol Obstet. 1992;251(1):1-7.Chambrone L, Pannuti CM, Guglielmetti MR, Chambrone LA. Evidence grade associating periodontitis with preterm birth and/or low birth weight: II: A systematic review of randomized trials evaluating the effects of periodontal treatment. J Clin Periodontol. 2011;38: 902–14.Cobo T, Kacerovsky M, Holst R, Hougaard D, Skogstrand K, Wennerholm U, et al. Intra-amniotic lammation predicts microbial invasion of the amniotic cavity but not spontaneous preterm delivery in preterm prelabor membrane rupture. Acta Obstet Gynecol Scand. 2012;91(8):930-5.Darmochwal-Kolarz D, Kludka-Sternik M, Tabarkiewicz J, Kolarz B, Rolinski J, LeszczynskaGorzelak B, et al. The predominance of Th17 lymphocytes and decreased number and function of Treg cells in preeclampsia; J Reprod Immunol. 2012;93(2):75-81.Department of Reproductive Health and Research (RHR), WHO - UNICEF. Low birth weight - Country, regional and global estimates. 2005. URL disponible en: http://www.who.int/reproductive-health/publications/low_birthweight/index.htmlDixon NG, Ebright D, Defrancesco MA, Hawkins RE. Orogenital contact: a cause of chorioamnionitis. Obstet Gynecol. 1994;84(4 Pt 2):654-5.Ercan E, Eratalay K, Deren O, Gur D, Ozyuncu O, Altun B et al. Evaluation of periodontal pathogens in amniotic fluid and the role of periodontal disease in pre-term birth and low birth weight. Acta Odontol Scand. 2013;71(3-4):553-9Erlebacher A. Immunology of the maternal-fetal interface. Annu Rev Immunol. 2013;31:387-11.Ernest JM, Wasilauskas B. Capnocytophaga in the amniotic fluid of a woman in preterm labor with intact membranes. Am J Obstet Gynecol. 1985;153(6):648-9.Esplin M, Romero R, Chaiworapongsa T, Kim Y, Edwin S, Gomez R et al. Monocyte chemotactic protein-1 is increased in the amniotic fluid of women who deliver preterm in the presence or absence of intra-amniotic infection. J Matern Fetal Neonatal Med. 2005;17(6):36573.Ferraz EM, Gray RH, Cunha TM. Determinants of preterm delivery and intrauterine growth retardation in north-east Brazil. Int J Epidemiol. 1990; 19(1):101-8.Galinsky R, Polglase GR, Hooper SB, Black MJ, Moss TJ. The Consequences of Chorioamnionitis: Preterm Birth and Effects on Development, J Pregnancy. 2013;2013:412831. Epub 2013 Mar 7.Garcia-Ruíz G, Flores-Espinosa P, Preciado-Martínez E, Bermejo-Martínez L, Espejel-Nuñez A, Estrada-Gutierrez G, et al. In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1β, TNFα, IL-6, IL-8, IL-10, MIP-1α, and MMP-9 in pre-labor human term placenta. Reprod Biol Endocrinol. 2015;13: 115.Gauthier S, Tétu A, Himaya E, Morand M, Chandad F, Rallu F et al. The origin of Fusobacterium nucleatum involved in intra-amniotic infection and preterm birth. J Matern Fetal Neonatal Med. 2011;24(11):1329-32.Gibbs RS, Duff P. Progress in pathogenesis and management of clinical intraamniotic infection. Am J Obstet Gynecol. 1991;164(5 Pt 1):1317-26.Goldenberg RL, Culhane JF. Infection as a cause of preterm birth. Clin Perinatol. 2003;30(4):677-700.Goldenberg RL, Culhane JF, Iams JD, Romero R. Epidemiology and causes of preterm birth. Lancet. 2008 Jan 5;371(9606):75-84.Gomes-Filho IS, Cruz SS, Rezende EJ, Dos Santos CA, Soledade KR, Magalhães MA, et al. Exposure measurement in the association between periodontal disease and prematurity/low birth weight. J Clin Periodontol. 2007;34(11):957-63.Gonzales-Marin C, Spratt DA, Millar MR, Simmonds M, Kempley ST, Allaker RP. Levels of periodontal pathogens in neonatal gastric aspirates and possible maternal sites of origin. Mol Oral Microbiol. 2011;26(5):277-90.Haffajee AD, Socransky SS, Patel MR, Song X. Microbial complexes in supragingival plaque. Oral Microbiol Immunol. 2008 Jun;23(3):196-205.Han YW, Shen T, Chung P, Buhimschi IA, Buhimschi CS. Uncultivated Bacteria as Etiologic Agents of Intra-Amniotic Inflammation Leading to Preterm Birth. J Clin Microbiol. 2009 Jan;47(1):38-47.Han YW, Fardini Y, Chen C, Iacampo KJ, Peraino VA, Shamonki JM, et al. Term stillbirth caused by oral Fusobacterium nucleatum. Obstet Gynecol 2010;115(2 Pt 2):442-5.Han, Y. W. Oral Health and Adverse Pregnancy Outcomes–What’s Next? J Dent Res. 2011;90(3):289-93.Hanna N, Bonifacio L, Weinberger B, Reddy P, Murphy S, Romero R et al. Evidence for Interleukin-10-Mediated Inhibition of Cyclo- oxygenase-2 Expression and Prostaglandin Production in Preterm Human Placenta. Am J Reprod Immunol. 2006;55(1):19-27Hasegawa-Nakamura K, Tateishi F, Nakamura T, Nakajima Y, Kawamata K, Douchi T, Hatae M, Noguchi K. (2011). The possible mechanism of preterm birth associated with periodontopathic Porphyromonas gingivalis. J Periodont Res 2011; 46: 497–504.Harmon QE, Engel SM, Olshan AF, Moran T, Stuebe AM, Luo J, et al. Association of Polymorphisms in Natural Killer Cell-Related Genes With Preterm Birth. Am J Epidemiol. 2013 Oct 15;178(8):1208-18.Hillier SL, Nugent RP, Eschenbach DA, Krohn MA, Gibbs RS, Martin DH, et al. Association between Bacterial Vaginosis and Preterm Delivery of a Low-Birth-Weight Infant. N Engl J Med. 1995;333(26):1737-42.Ide M, Papapanou PN. Epidemiology of association between maternal periodontal disease and adverse pregnancy outcomes – systematic review. J Periodontol. 2013;84(4 Suppl):S181-94.Inaba H, Kuboniwa M, Sugita H, Lamont R, Amano A. Identification of Signaling Pathways Mediating Cell Cycle Arrest and Apoptosis Induced by Porphyromonas gingivalis in Human Trophoblasts. Infect Immun. 2012;80(8):2847-57.Ito M, Nakashima A, Hidaka T, Okabe M, Bac N, Ina S et al. A role for IL-17 in induction of an inflammation at the fetomaternal interface in preterm labour. J Reprod Immunol. 2010;84(1):7585.Jacobsson B, Holst R, Wennerholm U, Andersson B, Lilja H, Hagberg H. Monocyte chemotactic protein-1 in cervical and amniotic fluid: relationship to microbial invasion of the amniotic cavity, intra-amniotic inflammation, and preterm delivery. Am J Obstet Gynecol. 2003;189(4):1161-7.Katz J, Chegini N, Shiverick K, Lamont R. Localization of P. gingivalis in Preterm Delivery Placenta. Jour J Dent Res. 2009;88(6):575-8.Kraus T, Engel S, Sperling R, Kellerman L, Lo Y, Wallenstein S et al. Characterizing the Pregnancy Immune Phenotype: Results of the Viral Immunity and Pregnancy (VIP) Study. J Clin Immunol. 2012;32(2):300-11.Lafaurie GI, Contreras A, Barón A, Botero J, Mayorga-Fayad I, Jaramillo A, et al. Demographic, clinical, and microbial aspects of chronic and aggressive periodontitis in Colombia: a multicenter study. J Periodontol. 2007;78(4):629-39.León R, Silva N, Ovalle A, Chaparro A, Ahumada A, Gajardo M et al. Detection of Porphyromonas gingivalis in the Amniotic Fluid in Pregnant Women With a Diagnosis of Threatened Premature Labor. J Periodontol. 2007;78(7):1249-55.Lin D, Moss K, Beck JD, Hefti, A, Offenbacher S. Persistently high levels of periodontal pathogens associated with preterm pregnancy outcome. J Periodontol. 2007;78(5):833-41.Madianos PN, Lieff S, Murtha AP, Boggess KA, Auten RL Jr, Beck JD, Offenbacher S. Maternal periodontitis and prematurity. Part II: Maternal infection and fetal exposure. Ann Periodontol. 2001;6(1):175-82.Madianos, P. N., Bobetsis, G. A., & Kinane, D. F. (2002). Is periodontitis associated with an increased risk of coronary heart disease and preterm and/or low birth weight births? Journal of Clinical Periodontology, 29(s3), 22–36. doi:10.1034/j.1600-051X.29.s3.2.Madianos PN, Bobetsis YA, Offenbacher S. Adverse pregnancy outcomes (APOs) and periodontal disease: pathogenic mechanisms. J Periodontol. 2013;84(4 Suppl):S170-80.Mahmoudi Rad M, Zafarghandi ASh, Amel Zabihi M, Tavallaee M, Mirdamadi Y. Identification of Candida species associated with vulvovaginal candidiasis by multiplex PCR. Infect Dis Obstet Gynecol. 2012;2012:872169.Marin C, Segura-Egea JJ, Martínez-Sahuquillo A, Bullón P. Correlation between infant birth weight and mother’s periodontal status. J Clin Periodontol. 2005;32(3):299-304.McCormick MC. The contribution of low birth weight to ant mortality and chilhood morbidity. N Engl J Med. 1985 (10);312(2):82-90.Mendz GL, Kaakoush NO, Quinlivan JA. Bacterial aetiological agents of intra-amniotic infections and preterm birth in pregnant women. Front Cell Infect Microbiol. 2013;3:58.Michalowicz BS, Durand R. Maternal periodontal disease and spontaneous preterm birth. Periodontol 2000. 2007;44:103-12.Michalowicz BS, Gustafsson A, Thumbigere-Math V, Buhlin K.The effects of periodontal treatment on pregnancy outcomes. J Periodontol. 2013 Apr;84(4 Suppl):S195-208.Mobini M, Mortazavi M, Nadi S, Zare-Bidaki M, Pourtalebi S, Arababadi MK. Significant roles played by interleukin-10 in outcome of pregnancy. Iran J Basic Med Sci. 2016;19(2):119-24.Moliterno LF, Monteiro B, Figueredo CM, Fischer RG. Association between periodontitis and low birth weight: a case–control study. J Clin Periodontol. 2005;32(8):886-90.Moreli JB, Corrêa-Silva S, Damasceno D, Sinzato Y, Lorenzon-Ojea A, Borbely Au, et al. Changes in the TNF-alpha/IL-10 ratio in hyperglycemia-associated pregnancies. Diabetes Res Clin Pract. 2015;107(3):362-9.Moore ML, Michielutte R, Meiss PJ, Ernest JM, Wells HB, Buescher PA. Etiology of low-birth weight birth: a population-based study. Prev Med 1994;23(6):793-9.Newton ER. Chorioamnionitis and intraamniotic infection. Clin Obstet Gynecol. 1993;36(4):795808.Noack M, Miossec P. Th17 and regulatory T cell balance Autoimmun Rev. 2014;13(6):668-77.Offenbacher S, Jared HL, O'Reilly PG, Wells SR, Salvi GE, Lawrence HP, et al. Potential pathogenic mechanisms of periodontitis associated pregnancy complications. Ann Periodontol. 1998;3(1):233-50.Pineda-Torres M, Flores-Espinosa P, Espejel-Nunez A, Estrada-Gutierrez G, Flores-Pliego A, Maida-Claros R et al. Evidence of an immunosuppressive effect of progesterone uponin vitrosecretion of proinflammatory and prodegradative factors in a model of choriodecidual infection. BJOG. 2015;122(13):1798-807.Prince A, Ma J, Kannan P, Alvarez M, Gisslen T, Harris R, et al. The placental membrane microbiome is altered among subjects with spontaneous preterm birth with and without chorioamnionitis. Am J Obstet Gynecol. 2016;214(5):627.e1-627.e16.Profamilia. Encuesta Nacional de Demografía y Salud ENDS 2005. Capítulo10. http://www.profamilia.org.co/encuestas/Profamilia/Profamilia/images/stories/ENDS2 05/general/capitulo_X.pdfProfamilia. Encuesta Nacional de Demografía y Salud ENDS 2010. Capítulo10. http://www.profamilia.org.co/encuestas/Profamilia/Profamilia/images/stories/PDFcapitulos/Capitulo-10.pdfRiewe S, Mans J, Hirano T, Katz J, Shiverick K, Brown T, et al. Human trophoblast responses to Porphyromonas gingivalis infection. Mol Oral Microbiol. 2010;25(4):252-9.Romero R, Chaemsaithong P, Docheva N, Korzeniewski S, Tarca A, Bhatti G, et al. Clinical chorioamnionitis at term V: umbilical cord plasma cytokine profile in the context of a systemic maternal inflammatory response. J Perinat Med. 2016;44(1):53-76.Saito S, Nakashima A, Shima T, Ito M. Th1 ⁄ Th2 ⁄ Th17 and regulatory T-cell paradigm in pregnancy. Am J Reprod Immunol. 2010;63(6):601-10.Santos-Pereira SA, Giraldo PC, Saba-Chujfi E, Amaral RL, Morais SS, Fachini AM, et al. Chronic periodontitis and pre-term labour in Brazilian pregnant women: an association to be analysed. J Clin Periodontol. 2007;34(3):208-13.Santa Cruz I, Herrera D, Martin C, Herrero A, Sanz M. Association between periodontal status and pre-term and/or low-birth weight in Spain: clinical and microbiological parameters. J Periodontal Res. 2013;48(4):443-51.Schatz F, Guzeloglu-Kayisli O, Arlier S, Kayisli U, Lockwood C. The role of decidual cells in uterine hemostasis, menstruation, inflammation, adverse pregnancy outcomes and abnormal uterine bleeding. Hum Reprod Update. 2016;22(4):497-515.Szaryńska M, Myśliwski A, Myśliwska J, Kmieć Z, Preis K, Zabul P. Cytokine profiles during delivery affect cord blood hematopoietic stem and progenitors cells. Cell Immunol. 2015;293(2):137-41.Toldi G, Rigó J, Stenczer B, Vásárhelyi B, Molvarec A. Increased Prevalence of IL-17-Producing Peripheral Blood Lymphocytes in Pre-eclampsia. Am J Reprod Immunol. 2011;66(3):223-9.Vacca P, Mingari M, Moretta L. Natural killer cells in human pregnancy. J Reprod Immunol. 2013;97(1):14-9.Vanterpool S, Been J, Houben M, Nikkels P, De Krijger R, Zimmermann L, et al. Porphyromonas gingivalis within Placental Villous Mesenchyme and Umbilical Cord Stroma Is Associated with Adverse Pregnancy Outcome. PLoS One. 2016;11(1):e0146157.Vettore MV1, Leão AT, Leal Mdo C, Feres M, Sheiham A. The relationship between periodontal disease and preterm low birthweight: clinical and microbiological results. J Periodontal Res. 2008;43(6):615-26.Wang C, SG Kang, J Lee, Z Sun and CH Kim. (2009) The roles of CCR6 in migration of Th-17 cells and regulation of effector T-cell balance in the gut. Mucosal Immunology. 2(2); 11.Williams CE, Davenport ES, Sterne JA, Sivapathasundaram V, Fearne JM, Curtis MA. Mechanisms of risk in preterm low-birthweight infants. Periodontol 2000. 2000 Jun;23:142-50.Wimmer G, Pihlstrom BL. A critical assessment of adverse pregnancy outcome and periodontal disease. J Clin Periodontol. 2008 Sep;35(8 Suppl):380-97.WHO. International statistical classification of diseases and related health problems. Instruction manual. Volume 2. 10th revision, edition 2010. Geneva, Switzerland. Retrieved from http://books.google.com/books?hl=es&lr=&id=Tw5eAtsatiUC&oi=fnd&pg=PA1&dq=International+Statistical+Classification+of+Diseases+and+Related+Health+Problems&ots=o1d4e2pJlK&sig=1eu2vtjopxMalKI9qgAFd7qRDBcWHO. Maternal Mortality in 2005. Estimates developed by WHO, UNICEF, UNFPA and The World Bank. Ginebra, 2007. URL disponible en: http://www.who.int/reproductivehealth/publications/maternal_mortality_2005/Xu M, Yamada M. Li M, Liu H, Chen SG, Han YW. FadA from Fusobacterium nucleatum utilizes both secreted and nonsecreted forms for funtional oligomerization for attachment and invasion of host cells. J Biol Chem. 2007;282(34):25000-9.Xiong X, Buekens P, Vastardis S, Yu SM. Periodontal disease and pregnancy outcomes: stateof-the-science. Obstet Gynecol Surv. 2007;62(9):605-15.Xiong X, Buekens P, Fraser WD, Beck J, Offenbacher S. Periodontal disease and adverse pregnancy outcomes: a systematic review. BJOG. 2006 Feb;113(2):135-43.Zaga-Clavellina V, Flores-Espinosa P, Pineda-Torres M, Sosa-González I, Vega-Sánchez R, Estrada-Gutierrez G, et al. Tissue-specific IL-10 secretion profile from term human fetal membranes stimulated with pathogenic microorganisms associated with preterm labor in a two- compartment tissue culture system. J Matern Fetal Neonatal Med. 2014;27(13):1320-7.Acceso cerradoinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbORIGINALPerfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amnióticaPerfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amnióticaPerfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amnióticaapplication/pdf6455167https://repositorio.unbosque.edu.co/bitstreams/803ed7d9-6925-4717-a3a2-c633ef789916/download1a2b58c99c09896e23844ebbcba6a499MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/a8f70042-82ec-4b44-883c-52edf9a56654/download8a4605be74aa9ea9d79846c1fba20a33MD54Gomez_Peña_Luz_Amparo_2017_Anexos (2).pdfGomez_Peña_Luz_Amparo_2017_Anexos (2).pdfAnexosapplication/pdf6628211https://repositorio.unbosque.edu.co/bitstreams/56e9b91e-ddc0-4a55-a625-88a185f94d58/download53958a7c941c7288a7c33bc4e59de4f2MD55THUMBNAILPortada.pngPortada.pngPortadaimage/png28957https://repositorio.unbosque.edu.co/bitstreams/33504c69-a46d-4ac9-9cd1-cfed4831a720/download81a174a936f9bf341e21160656df8778MD56Perfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica.jpgPerfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica.jpgIM Thumbnailimage/jpeg4466https://repositorio.unbosque.edu.co/bitstreams/7cbe7c14-b8e0-4e28-b3fe-6495dca41e54/download7366b787fbf4ae2b5be61643220e4b05MD57TEXTPerfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica.txtPerfiles de citocinas en tejido placentario de mujeres con resultados adversos del embarazo asociadas a infección intra-amniótica.txtExtracted texttext/plain101697https://repositorio.unbosque.edu.co/bitstreams/c737c057-8c5b-4dc9-a9fc-01a67293a859/download8411dc60a2bca4415fb398c476b70c35MD5820.500.12495/10100oai:repositorio.unbosque.edu.co:20.500.12495/101002024-02-06 22:41:09.31restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |