Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation
Purpose: This research evaluated clinical, histological, and radiological osseous regeneration in a critical-sized bilateral cortico-medullary osseous defect in model rabbits from New Zealand after receiving a hydroxyapatite matrix and polylactic polyglycolic acid (HA/PLGA) implanted with human dent...
- Autores:
-
Gutiérrez-Quintero, Juan G.
Durán Riveros, Juan Y.
Martínez Valbuena, Carlos A.
Pedraza Alonso, Sofía
Munévar Niño, Juan Carlos
Viafara García, Sergio Marino
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/3561
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/3561
https://doi.org/10.1007/s10006-020-00862-7
https://repositorio.unbosque.edu.co
- Palabra clave:
- Medicina regenerativa
Andamios del tejido
Huesos faciales
Bone regeneration
Mesenchymal stem cells
Xenograft
- Rights
- openAccess
- License
- Acceso abierto
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UNBOSQUE2 |
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Repositorio U. El Bosque |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation |
dc.title.translated.spa.fl_str_mv |
Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation |
title |
Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation |
spellingShingle |
Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation Medicina regenerativa Andamios del tejido Huesos faciales Bone regeneration Mesenchymal stem cells Xenograft |
title_short |
Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation |
title_full |
Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation |
title_fullStr |
Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation |
title_full_unstemmed |
Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation |
title_sort |
Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluation |
dc.creator.fl_str_mv |
Gutiérrez-Quintero, Juan G. Durán Riveros, Juan Y. Martínez Valbuena, Carlos A. Pedraza Alonso, Sofía Munévar Niño, Juan Carlos Viafara García, Sergio Marino |
dc.contributor.author.none.fl_str_mv |
Gutiérrez-Quintero, Juan G. Durán Riveros, Juan Y. Martínez Valbuena, Carlos A. Pedraza Alonso, Sofía Munévar Niño, Juan Carlos Viafara García, Sergio Marino |
dc.contributor.orcid.none.fl_str_mv |
Viafara García, Sergio Marino [0000-0002-4208-1422] Munévar Niño, Juan Carlos [0000-0002-8482-2962] |
dc.subject.decs.spa.fl_str_mv |
Medicina regenerativa Andamios del tejido Huesos faciales |
topic |
Medicina regenerativa Andamios del tejido Huesos faciales Bone regeneration Mesenchymal stem cells Xenograft |
dc.subject.keywords.spa.fl_str_mv |
Bone regeneration Mesenchymal stem cells Xenograft |
description |
Purpose: This research evaluated clinical, histological, and radiological osseous regeneration in a critical-sized bilateral cortico-medullary osseous defect in model rabbits from New Zealand after receiving a hydroxyapatite matrix and polylactic polyglycolic acid (HA/PLGA) implanted with human dental pulp stem cells (DPSCs). Methods: Eight New Zealand rabbits with bilateral mandibular critical-sized defects were performed where one side was treated with an HA/PLGA/DPSC matrix and the other side only with an HA/PLGA matrix for 4 weeks. Results: An osseointegration was clinically observed as well as a reduction of 70% of the surgical lumen on one side and a 35% on the other. Histologically, there was neo-bone formation in HA/PLGA/DPSC scaffold and angiogenesis. A bone radiodensity (RD) of 80% was radiologically observed achieving density levels similar to mandibular bone, while the treatment with HA/PLGA matrix achieves RD levels of 40% on its highest peaks. Conclusions: HA/PLGA/DPSC scaffold was an effective in vivo method for mandibular bone regeneration in critical-sized defects induced on rabbit models. |
publishDate |
2020 |
dc.date.accessioned.none.fl_str_mv |
2020-07-21T20:34:30Z |
dc.date.available.none.fl_str_mv |
2020-07-21T20:34:30Z |
dc.date.issued.none.fl_str_mv |
2020 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.none.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.issn.none.fl_str_mv |
1865-1569 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/3561 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1007/s10006-020-00862-7 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
https://repositorio.unbosque.edu.co |
identifier_str_mv |
1865-1569 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque |
url |
http://hdl.handle.net/20.500.12495/3561 https://doi.org/10.1007/s10006-020-00862-7 https://repositorio.unbosque.edu.co |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Oral and maxillofacial surgery, 1865-1569, 2020 |
dc.relation.uri.none.fl_str_mv |
https://link.springer.com/article/10.1007%2Fs10006-020-00862-7 |
dc.rights.local.spa.fl_str_mv |
Acceso abierto |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Acceso abierto |
dc.rights.creativecommons.none.fl_str_mv |
2020 |
rights_invalid_str_mv |
Acceso abierto http://purl.org/coar/access_right/c_abf2 2020 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Springer Nature |
dc.publisher.journal.spa.fl_str_mv |
Oral and maxillofacial surgery |
institution |
Universidad El Bosque |
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Gutiérrez-Quintero, Juan G.Durán Riveros, Juan Y.Martínez Valbuena, Carlos A.Pedraza Alonso, SofíaMunévar Niño, Juan CarlosViafara García, Sergio MarinoViafara García, Sergio Marino [0000-0002-4208-1422]Munévar Niño, Juan Carlos [0000-0002-8482-2962]2020-07-21T20:34:30Z2020-07-21T20:34:30Z20201865-1569http://hdl.handle.net/20.500.12495/3561https://doi.org/10.1007/s10006-020-00862-7instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengSpringer NatureOral and maxillofacial surgeryOral and maxillofacial surgery, 1865-1569, 2020https://link.springer.com/article/10.1007%2Fs10006-020-00862-7Critical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluationCritical-sized mandibular defect reconstruction using human dental pulp stem cells in a xenograft model-clinical, radiological, and histological evaluationArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Medicina regenerativaAndamios del tejidoHuesos facialesBone regenerationMesenchymal stem cellsXenograftPurpose: This research evaluated clinical, histological, and radiological osseous regeneration in a critical-sized bilateral cortico-medullary osseous defect in model rabbits from New Zealand after receiving a hydroxyapatite matrix and polylactic polyglycolic acid (HA/PLGA) implanted with human dental pulp stem cells (DPSCs). Methods: Eight New Zealand rabbits with bilateral mandibular critical-sized defects were performed where one side was treated with an HA/PLGA/DPSC matrix and the other side only with an HA/PLGA matrix for 4 weeks. Results: An osseointegration was clinically observed as well as a reduction of 70% of the surgical lumen on one side and a 35% on the other. Histologically, there was neo-bone formation in HA/PLGA/DPSC scaffold and angiogenesis. A bone radiodensity (RD) of 80% was radiologically observed achieving density levels similar to mandibular bone, while the treatment with HA/PLGA matrix achieves RD levels of 40% on its highest peaks. Conclusions: HA/PLGA/DPSC scaffold was an effective in vivo method for mandibular bone regeneration in critical-sized defects induced on rabbit models.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2020ORIGINALGutiérrez-Quintero, JG, Durán Riveros, JY, Martínez Valbuena, CA_2020.pdfGutiérrez-Quintero, JG, Durán Riveros, JY, Martínez Valbuena, CA_2020.pdfapplication/pdf8119828https://repositorio.unbosque.edu.co/bitstreams/aac8700b-4d80-4b7f-81b4-a274338b300b/download378ec9a0f4d4544b153d5848791f7f02MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/f3bab9a8-01c5-472a-b5a2-cc1c5a5406f3/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILGutiérrez-Quintero, JG, Durán Riveros, JY, Martínez Valbuena, CA_2020.pdf.jpgGutiérrez-Quintero, JG, Durán Riveros, JY, Martínez Valbuena, CA_2020.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/f4ef87ba-bd07-4c28-a7d0-4154b7d82d23/download7210a811635d1799e7c05fee5d259be7MD53Gutiérrez-Quintero, JG, Durán Riveros, JY, Martínez Valbuena, CA_2020.pdf.jpgGutiérrez-Quintero, JG, Durán Riveros, JY, Martínez Valbuena, CA_2020.pdf.jpgIM Thumbnailimage/jpeg9643https://repositorio.unbosque.edu.co/bitstreams/cf1d7380-c096-45e1-bf6a-6fd9ef4fd78b/download776667f8b48adc2fe25197e14fa042e4MD54TEXTGutiérrez-Quintero, JG, Durán Riveros, JY, Martínez Valbuena, CA_2020.pdf.txtGutiérrez-Quintero, JG, Durán Riveros, JY, Martínez Valbuena, CA_2020.pdf.txtExtracted texttext/plain30926https://repositorio.unbosque.edu.co/bitstreams/0a2bd752-6d80-4953-ac91-0625e2ed39bc/download7cb769be7b11d95ca9cbbeeb838a8b8aMD5520.500.12495/3561oai:repositorio.unbosque.edu.co:20.500.12495/35612024-02-06 22:51:15.617restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |