A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineage

ABSTRACT Ceftaroline (CPT) is a broad-spectrum agent with potent activity against methicillin-resistant Staphylococcus aureus (MRSA). The sequence type 5 (ST5) Chilean-Cordobés clone, associated with CPT nonsusceptibility, is dominant in Chile, a region with high rates of MRSA infections. Here, we a...

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Autores:
Khan, Ayesha
Rivas, Lina M.
Spencer, Maria
Martinez, Rodrigo
Lam, Marusella
Rojas, Pamela
Porte, Lorena
Silva, Francisco
Braun, Stephanie
Valdivieso, Francisca
Mvlhauser, Margareta
Lafourcade, Mónica
Miller, William
García, Patricia
Arias, Cesar
Munita, José M.
Tipo de recurso:
Fecha de publicación:
2019
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/2020
Acceso en línea:
http://hdl.handle.net/20.500.12495/2020
https://doi.org/10.1128/JCM.00798-19
Palabra clave:
Staphylococcus aureus resistente a meticilina
Bacilos grampositivos formadores de endosporas
Pruebas Antimicrobianas de difusión por disco
Ceftaroline
Disk diffusion
Etest
Breakpoints
Rights
License
Attribution 4.0 International
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network_name_str Repositorio U. El Bosque
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dc.title.spa.fl_str_mv A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineage
dc.title.translated.none.fl_str_mv A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineage
title A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineage
spellingShingle A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineage
Staphylococcus aureus resistente a meticilina
Bacilos grampositivos formadores de endosporas
Pruebas Antimicrobianas de difusión por disco
Ceftaroline
Disk diffusion
Etest
Breakpoints
title_short A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineage
title_full A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineage
title_fullStr A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineage
title_full_unstemmed A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineage
title_sort A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineage
dc.creator.fl_str_mv Khan, Ayesha
Rivas, Lina M.
Spencer, Maria
Martinez, Rodrigo
Lam, Marusella
Rojas, Pamela
Porte, Lorena
Silva, Francisco
Braun, Stephanie
Valdivieso, Francisca
Mvlhauser, Margareta
Lafourcade, Mónica
Miller, William
García, Patricia
Arias, Cesar
Munita, José M.
dc.contributor.author.none.fl_str_mv Khan, Ayesha
Rivas, Lina M.
Spencer, Maria
Martinez, Rodrigo
Lam, Marusella
Rojas, Pamela
Porte, Lorena
Silva, Francisco
Braun, Stephanie
Valdivieso, Francisca
Mvlhauser, Margareta
Lafourcade, Mónica
Miller, William
García, Patricia
Arias, Cesar
Munita, José M.
dc.subject.decs.spa.fl_str_mv Staphylococcus aureus resistente a meticilina
Bacilos grampositivos formadores de endosporas
Pruebas Antimicrobianas de difusión por disco
topic Staphylococcus aureus resistente a meticilina
Bacilos grampositivos formadores de endosporas
Pruebas Antimicrobianas de difusión por disco
Ceftaroline
Disk diffusion
Etest
Breakpoints
dc.subject.keywords.spa.fl_str_mv Ceftaroline
Disk diffusion
Etest
Breakpoints
description ABSTRACT Ceftaroline (CPT) is a broad-spectrum agent with potent activity against methicillin-resistant Staphylococcus aureus (MRSA). The sequence type 5 (ST5) Chilean-Cordobés clone, associated with CPT nonsusceptibility, is dominant in Chile, a region with high rates of MRSA infections. Here, we assessed the in vitro activity of CPT against a collection of MRSA isolates collected between 1999 and 2018 from nine hospitals (n 320) and community settings (n 41) in Santiago, Chile, and evaluated performance across testing methodologies. We found that our hospitalassociated isolates exhibited higher CPT MIC distributions (MIC50 and MIC90 of 2 mg/liter) than the community isolates (MIC50 and MIC90 of 0.5 mg/liter), a finding that was consistent across time and independent of the culture source. High proportions (64%) of isolates were CPT nonsusceptible despite the absence of CPT use in Chile. Across methodologies, the Etest underestimated the MIC relative to the gold standard broth microdilution (BMD) test (MIC50 and MIC90 of 1 and 1.5 mg/liter, respectively). There was low (51%) categorical agreement (CA) between Etest and BMD results across CLSI and EUCAST breakpoints. The recent revision of CLSI guidelines abolished “very major error” (VME) from the previous guidelines (81%), which perform similarly to the EUCAST guidelines. The level of concordance between CLSI and EUCAST for BMD testing and Etest was 95%. Disk diffusion performed poorly relative to BMD under CLSI (CA, 55%) and EUCAST (CA, 36%) guidelines. Comparison of EUCAST to CLSI for disk diffusion (with EUCAST used as the reference) showed low agreement (CA, 25%; VME, 70%). In summary, CPT-nonsusceptible MRSA are dominant in clinical settings in Chile. Our results provide data to support the reevaluation of CPT breakpoints and to improve agreement across methodologies and agencies.
publishDate 2019
dc.date.issued.none.fl_str_mv 2019
dc.date.accessioned.none.fl_str_mv 2020-03-09T14:24:49Z
dc.date.available.none.fl_str_mv 2020-03-09T14:24:49Z
dc.type.spa.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.local.spa.fl_str_mv artículo
dc.identifier.issn.none.fl_str_mv 0095-1137
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/2020
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1128/JCM.00798-19
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
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identifier_str_mv 0095-1137
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
repourl:https://repositorio.unbosque.edu.co
url http://hdl.handle.net/20.500.12495/2020
https://doi.org/10.1128/JCM.00798-19
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Journa of Clinical Microbiology, 0095-1137, Vol. 57, Nro. 9, 2019, p.1-11
dc.relation.uri.none.fl_str_mv https://jcm.asm.org/content/57/9/e00798-19
dc.rights.*.fl_str_mv Attribution 4.0 International
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.local.spa.fl_str_mv Acceso abierto
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf183
dc.rights.creativecommons.none.fl_str_mv 2019
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
Acceso abierto
http://purl.org/coar/access_right/c_abf183
2019
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv American Society for Microbiology
dc.publisher.journal.spa.fl_str_mv Journa of Clinical Microbiology
institution Universidad El Bosque
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spelling Khan, AyeshaRivas, Lina M.Spencer, MariaMartinez, RodrigoLam, MarusellaRojas, PamelaPorte, LorenaSilva, FranciscoBraun, StephanieValdivieso, FranciscaMvlhauser, MargaretaLafourcade, MónicaMiller, WilliamGarcía, PatriciaArias, CesarMunita, José M.2020-03-09T14:24:49Z2020-03-09T14:24:49Z20190095-1137http://hdl.handle.net/20.500.12495/2020https://doi.org/10.1128/JCM.00798-19instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengAmerican Society for MicrobiologyJourna of Clinical MicrobiologyJourna of Clinical Microbiology, 0095-1137, Vol. 57, Nro. 9, 2019, p.1-11https://jcm.asm.org/content/57/9/e00798-19Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Acceso abiertohttp://purl.org/coar/access_right/c_abf1832019http://purl.org/coar/access_right/c_abf2A multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineageA multicenter study to evaluate ceftaroline breakpoints: Performance in an area with high prevalence of methicillin- resistant Staphylococcus aureus sequence type 5 lineagearticleartículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Staphylococcus aureus resistente a meticilinaBacilos grampositivos formadores de endosporasPruebas Antimicrobianas de difusión por discoCeftarolineDisk diffusionEtestBreakpointsABSTRACT Ceftaroline (CPT) is a broad-spectrum agent with potent activity against methicillin-resistant Staphylococcus aureus (MRSA). The sequence type 5 (ST5) Chilean-Cordobés clone, associated with CPT nonsusceptibility, is dominant in Chile, a region with high rates of MRSA infections. Here, we assessed the in vitro activity of CPT against a collection of MRSA isolates collected between 1999 and 2018 from nine hospitals (n 320) and community settings (n 41) in Santiago, Chile, and evaluated performance across testing methodologies. We found that our hospitalassociated isolates exhibited higher CPT MIC distributions (MIC50 and MIC90 of 2 mg/liter) than the community isolates (MIC50 and MIC90 of 0.5 mg/liter), a finding that was consistent across time and independent of the culture source. High proportions (64%) of isolates were CPT nonsusceptible despite the absence of CPT use in Chile. Across methodologies, the Etest underestimated the MIC relative to the gold standard broth microdilution (BMD) test (MIC50 and MIC90 of 1 and 1.5 mg/liter, respectively). There was low (51%) categorical agreement (CA) between Etest and BMD results across CLSI and EUCAST breakpoints. The recent revision of CLSI guidelines abolished “very major error” (VME) from the previous guidelines (81%), which perform similarly to the EUCAST guidelines. The level of concordance between CLSI and EUCAST for BMD testing and Etest was 95%. Disk diffusion performed poorly relative to BMD under CLSI (CA, 55%) and EUCAST (CA, 36%) guidelines. Comparison of EUCAST to CLSI for disk diffusion (with EUCAST used as the reference) showed low agreement (CA, 25%; VME, 70%). In summary, CPT-nonsusceptible MRSA are dominant in clinical settings in Chile. Our results provide data to support the reevaluation of CPT breakpoints and to improve agreement across methodologies and agencies.CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8908https://repositorio.unbosque.edu.co/bitstreams/675292a6-a264-4e8c-b70a-59be7d72a0d8/download0175ea4a2d4caec4bbcc37e300941108MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/42f171a4-63e5-4b02-8f58-a781ba32ce5a/download8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALKhan, Ayesha.pdfKhan, Ayesha.pdfapplication/pdf527817https://repositorio.unbosque.edu.co/bitstreams/3ad987d0-09a2-46d9-ba1e-75da48e803b7/download7c50a9a2622c7f76e8e74eb7b6c0bc03MD51THUMBNAILKhan, Ayesha.pdf.jpgKhan, Ayesha.pdf.jpgIM Thumbnailimage/jpeg12733https://repositorio.unbosque.edu.co/bitstreams/c040f657-b6bd-4e24-adc9-d0481de6525c/download123a42d4cec5d4998da9f4fd6cec0de9MD54TEXTKhan, Ayesha.pdf.txtKhan, Ayesha.pdf.txtExtracted texttext/plain52569https://repositorio.unbosque.edu.co/bitstreams/1366a3a0-ec44-4375-9dc9-096de3fd1e36/downloadbdd02f267bfd0aa27274109907daeb31MD5520.500.12495/2020oai:repositorio.unbosque.edu.co:20.500.12495/20202024-02-07 02:42:06.822http://creativecommons.org/licenses/by/4.0/Attribution 4.0 Internationalopen.accesshttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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