Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix
The flavone 3,5,7-trihydroxy-6-methoxyflavone was isolated from leaf extracts of Chromolaena leivensis (Hieron), commonly named plant of cancer. It was identified based on their physicochemical propierties and spectroscopic data. This compound presented a methoxylation at C6, this is uncommon in fla...
- Autores:
-
Torrenegra, Rubén Darío
Rodríguez, J.
RODRIGUEZ AGUIRRE, OSCAR EDUARDO
Palau, Victoria
Mendez-Callejas, Gina Marcela
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/3515
- Palabra clave:
- Chromolaena
Plantas medicinales
Citotoxinas
Cancer cells
Chromolaena leivensis
antiproliferative activity
- Rights
- openAccess
- License
- Acceso abierto
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oai:repositorio.unbosque.edu.co:20.500.12495/3515 |
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UNBOSQUE2 |
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Repositorio U. El Bosque |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix |
dc.title.translated.spa.fl_str_mv |
Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix |
title |
Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix |
spellingShingle |
Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix Chromolaena Plantas medicinales Citotoxinas Cancer cells Chromolaena leivensis antiproliferative activity |
title_short |
Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix |
title_full |
Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix |
title_fullStr |
Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix |
title_full_unstemmed |
Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix |
title_sort |
Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix |
dc.creator.fl_str_mv |
Torrenegra, Rubén Darío Rodríguez, J. RODRIGUEZ AGUIRRE, OSCAR EDUARDO Palau, Victoria Mendez-Callejas, Gina Marcela |
dc.contributor.author.none.fl_str_mv |
Torrenegra, Rubén Darío Rodríguez, J. RODRIGUEZ AGUIRRE, OSCAR EDUARDO Palau, Victoria Mendez-Callejas, Gina Marcela |
dc.contributor.orcid.none.fl_str_mv |
Rodríguez Aguirre, Oscar Eduardo [0000-0002-5934-0451] |
dc.subject.decs.spa.fl_str_mv |
Chromolaena Plantas medicinales Citotoxinas |
topic |
Chromolaena Plantas medicinales Citotoxinas Cancer cells Chromolaena leivensis antiproliferative activity |
dc.subject.keywords.spa.fl_str_mv |
Cancer cells Chromolaena leivensis antiproliferative activity |
description |
The flavone 3,5,7-trihydroxy-6-methoxyflavone was isolated from leaf extracts of Chromolaena leivensis (Hieron), commonly named plant of cancer. It was identified based on their physicochemical propierties and spectroscopic data. This compound presented a methoxylation at C6, this is uncommon in flavonoids and which may confer some specificity of its biological activity. The cytotoxic activity of this flavonoid was determined on PC3 (prostate), MDA- MB-231 (breast), HT29 (colon), SiHa (cervix) and A549 (lung) cancer cells, using MTT assay, to assess if the flavonoid contributes to the anticancer activity previously proposed for Chromolaena leivensis. The cytotoxic activity of the 3,5,7-trihydroxy-6- methoxy flavone was similar to that obtained for the flavonoid quercetin but was low compared with the positive control vincristine sulphate. The better value of the inhibitory concentration of fifty percent (IC50) 150 µM, was achieved on SiHa cell line, while the lower activity: 4008 µM, was obtained on HT29 cancer cell line. However, severe morphological changes were detected on cytoskeleton and nucleus of the SiHa cells detected by immunofluorescence microscopy analysis of cells exposed to the half of the IC50 concentration obtained for the flavonoid. Data indicate that the flavonoid contributes to the anticancer activity of the extracts of leaves from Chromolaena leivensis, and could broadening the spectrum of flavonoids activity against various types of cancer non hormone-dependent. |
publishDate |
2016 |
dc.date.issued.none.fl_str_mv |
2016 |
dc.date.accessioned.none.fl_str_mv |
2020-07-15T22:34:03Z |
dc.date.available.none.fl_str_mv |
2020-07-15T22:34:03Z |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.none.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.issn.none.fl_str_mv |
1827-8620 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/3515 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
https://repositorio.unbosque.edu.co |
identifier_str_mv |
1827-8620 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque |
url |
http://hdl.handle.net/20.500.12495/3515 https://repositorio.unbosque.edu.co |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Pharmacologyonline, 1827-8620, Vol, 2016, Nro.1 , 2016, p. 7-11 |
dc.relation.uri.none.fl_str_mv |
https://pharmacologyonline.silae.it/files/archives/2016/vol1/PhOL_2016_1_A002_02_Torrenegra_7_11.pdf |
dc.rights.local.spa.fl_str_mv |
Acceso abierto |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Acceso abierto |
dc.rights.creativecommons.none.fl_str_mv |
2016-04-30 |
rights_invalid_str_mv |
Acceso abierto http://purl.org/coar/access_right/c_abf2 2016-04-30 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
SILAE |
dc.publisher.journal.spa.fl_str_mv |
Pharmacologyonline |
institution |
Universidad El Bosque |
bitstream.url.fl_str_mv |
https://repositorio.unbosque.edu.co/bitstreams/0985252d-2def-4eaa-94d0-3d64449c49f6/download https://repositorio.unbosque.edu.co/bitstreams/f55657c4-1f35-40a0-856a-72d2ab536205/download https://repositorio.unbosque.edu.co/bitstreams/04a6f8e5-490b-4d54-9879-1966306a0a5a/download https://repositorio.unbosque.edu.co/bitstreams/a7c42e76-6f51-4988-86c3-7cc68230481c/download |
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Torrenegra, Rubén DaríoRodríguez, J.RODRIGUEZ AGUIRRE, OSCAR EDUARDOPalau, VictoriaMendez-Callejas, Gina MarcelaRodríguez Aguirre, Oscar Eduardo [0000-0002-5934-0451]2020-07-15T22:34:03Z2020-07-15T22:34:03Z20161827-8620http://hdl.handle.net/20.500.12495/3515instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengSILAEPharmacologyonlinePharmacologyonline, 1827-8620, Vol, 2016, Nro.1 , 2016, p. 7-11https://pharmacologyonline.silae.it/files/archives/2016/vol1/PhOL_2016_1_A002_02_Torrenegra_7_11.pdfAntiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervixAntiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervixArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85ChromolaenaPlantas medicinalesCitotoxinasCancer cellsChromolaena leivensisantiproliferative activityThe flavone 3,5,7-trihydroxy-6-methoxyflavone was isolated from leaf extracts of Chromolaena leivensis (Hieron), commonly named plant of cancer. It was identified based on their physicochemical propierties and spectroscopic data. This compound presented a methoxylation at C6, this is uncommon in flavonoids and which may confer some specificity of its biological activity. The cytotoxic activity of this flavonoid was determined on PC3 (prostate), MDA- MB-231 (breast), HT29 (colon), SiHa (cervix) and A549 (lung) cancer cells, using MTT assay, to assess if the flavonoid contributes to the anticancer activity previously proposed for Chromolaena leivensis. The cytotoxic activity of the 3,5,7-trihydroxy-6- methoxy flavone was similar to that obtained for the flavonoid quercetin but was low compared with the positive control vincristine sulphate. The better value of the inhibitory concentration of fifty percent (IC50) 150 µM, was achieved on SiHa cell line, while the lower activity: 4008 µM, was obtained on HT29 cancer cell line. However, severe morphological changes were detected on cytoskeleton and nucleus of the SiHa cells detected by immunofluorescence microscopy analysis of cells exposed to the half of the IC50 concentration obtained for the flavonoid. Data indicate that the flavonoid contributes to the anticancer activity of the extracts of leaves from Chromolaena leivensis, and could broadening the spectrum of flavonoids activity against various types of cancer non hormone-dependent.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2016-04-30ORIGINALTorrenegra, R.D.; Rodríguez, J_2016.pdfTorrenegra, R.D.; Rodríguez, J_2016.pdfapplication/pdf773863https://repositorio.unbosque.edu.co/bitstreams/0985252d-2def-4eaa-94d0-3d64449c49f6/downloaded8bb72c821b55f480dafc8ea079b382MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/f55657c4-1f35-40a0-856a-72d2ab536205/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILTorrenegra, R.D.; Rodríguez, J_2016.pdf.jpgTorrenegra, R.D.; Rodríguez, J_2016.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/04a6f8e5-490b-4d54-9879-1966306a0a5a/download7210a811635d1799e7c05fee5d259be7MD53TEXTTorrenegra, R.D.; Rodríguez, J_2016.pdf.txtTorrenegra, R.D.; Rodríguez, J_2016.pdf.txtExtracted texttext/plain18729https://repositorio.unbosque.edu.co/bitstreams/a7c42e76-6f51-4988-86c3-7cc68230481c/downloadc15317618ebbc53e8cf7c34df081c6afMD5420.500.12495/3515oai:repositorio.unbosque.edu.co:20.500.12495/35152024-02-07 01:40:18.599restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |