Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. Oralis

We investigated the ability of several recent clinical viridans group streptococci (VGS) bloodstream isolates (Streptococcus mitis/S. oralis subgroup) from daptomycin (DAP)-naive patients to develop DAP resistance in vitro. All strains rapidly developed high-level and stable DAP resistance. Substitu...

Full description

Autores:
Tran, Truc T.
Mishra, Nagendra Nath
Seepersaud, Ravin
Diaz, Lorena
Dinh, An Q.
García-De-La-Mària, Cristina
Rybak, Michael Joseph
Miró, José María
Shelburne, Samuel A.
Shelburne, Samuel A.
Sullam, Paul M.
Bayer, Arnold S.
Arias, César A.
Tipo de recurso:
Fecha de publicación:
2019
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/2170
Acceso en línea:
http://hdl.handle.net/20.500.12495/2170
https://doi.org/10.1128/AAC.01531-18
Palabra clave:
Streptococcus miti
Cardiolipinas
Daptomicina
CdsA
PgsA
Daptomycin resistance
Rights
License
Acceso cerrado
id UNBOSQUE2_08b2af1f6a08610887d01957fe02d1a1
oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/2170
network_acronym_str UNBOSQUE2
network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.spa.fl_str_mv Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. Oralis
title Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. Oralis
spellingShingle Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. Oralis
Streptococcus miti
Cardiolipinas
Daptomicina
CdsA
PgsA
Daptomycin resistance
title_short Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. Oralis
title_full Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. Oralis
title_fullStr Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. Oralis
title_full_unstemmed Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. Oralis
title_sort Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. Oralis
dc.creator.fl_str_mv Tran, Truc T.
Mishra, Nagendra Nath
Seepersaud, Ravin
Diaz, Lorena
Dinh, An Q.
García-De-La-Mària, Cristina
Rybak, Michael Joseph
Miró, José María
Shelburne, Samuel A.
Shelburne, Samuel A.
Sullam, Paul M.
Bayer, Arnold S.
Arias, César A.
dc.contributor.author.none.fl_str_mv Tran, Truc T.
Mishra, Nagendra Nath
Seepersaud, Ravin
Diaz, Lorena
Dinh, An Q.
García-De-La-Mària, Cristina
Rybak, Michael Joseph
Miró, José María
Shelburne, Samuel A.
Shelburne, Samuel A.
Sullam, Paul M.
Bayer, Arnold S.
Arias, César A.
dc.subject.decs.spa.fl_str_mv Streptococcus miti
Cardiolipinas
Daptomicina
topic Streptococcus miti
Cardiolipinas
Daptomicina
CdsA
PgsA
Daptomycin resistance
dc.subject.keywords.spa.fl_str_mv CdsA
PgsA
Daptomycin resistance
description We investigated the ability of several recent clinical viridans group streptococci (VGS) bloodstream isolates (Streptococcus mitis/S. oralis subgroup) from daptomycin (DAP)-naive patients to develop DAP resistance in vitro. All strains rapidly developed high-level and stable DAP resistance. Substitutions in two enzymes involved in the cardiolipin biosynthesis pathway were identified, i.e., CdsA (phosphatidate cytidylyltransferase) and PgsA (CDP-diacylglycerol-glycerol-3-phosphate-3phosphatidyltransferase). These mutations were associated with complete disappearance of phosphatidylglycerol and cardiolipin from cell membranes. DAP interactions with the cell membrane differed in isolates with PgsA versus CdsA substitutions
publishDate 2019
dc.date.issued.none.fl_str_mv 2019
dc.date.accessioned.none.fl_str_mv 2020-03-27T17:11:57Z
dc.date.available.none.fl_str_mv 2020-03-27T17:11:57Z
dc.type.spa.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.local.spa.fl_str_mv artículo
dc.identifier.issn.none.fl_str_mv 1098-6596
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/2170
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1128/AAC.01531-18
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv repourl:https://repositorio.unbosque.edu.co
identifier_str_mv 1098-6596
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
repourl:https://repositorio.unbosque.edu.co
url http://hdl.handle.net/20.500.12495/2170
https://doi.org/10.1128/AAC.01531-18
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Antimicrobial agents and chemotherapy, 1098-6596, Vol. 63, Nro. 2, 2019, p. e01531-18
dc.relation.uri.none.fl_str_mv https://aac.asm.org/content/63/2/e01531-18.abstract
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.local.spa.fl_str_mv Acceso cerrado
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf328
dc.rights.creativecommons.none.fl_str_mv 2019
rights_invalid_str_mv Acceso cerrado
http://purl.org/coar/access_right/c_abf328
2019
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv American society for microbiology
dc.publisher.journal.spa.fl_str_mv Antimicrobial agents and chemotherapy
institution Universidad El Bosque
bitstream.url.fl_str_mv https://repositorio.unbosque.edu.co/bitstreams/602c9075-23ef-491d-ba34-ec4cca9d1605/download
https://repositorio.unbosque.edu.co/bitstreams/00cb14a1-d8fb-41db-857d-8af6f6dfa279/download
https://repositorio.unbosque.edu.co/bitstreams/9fdae48d-77ed-4c56-a821-821768cb6ac8/download
https://repositorio.unbosque.edu.co/bitstreams/ecb2813e-5238-4569-9955-18df3be0be9a/download
bitstream.checksum.fl_str_mv 8a4605be74aa9ea9d79846c1fba20a33
7210a811635d1799e7c05fee5d259be7
211eec4c4566cb9d0ddc2e71a7cf5369
53eb7dd0223dd1e075227ff68d10ff4b
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad El Bosque
repository.mail.fl_str_mv bibliotecas@biteca.com
_version_ 1814100837564153856
spelling Tran, Truc T.Mishra, Nagendra NathSeepersaud, RavinDiaz, LorenaDinh, An Q.García-De-La-Mària, CristinaRybak, Michael JosephMiró, José MaríaShelburne, Samuel A.Shelburne, Samuel A.Sullam, Paul M.Bayer, Arnold S.Arias, César A.2020-03-27T17:11:57Z2020-03-27T17:11:57Z20191098-6596http://hdl.handle.net/20.500.12495/2170https://doi.org/10.1128/AAC.01531-18instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengAmerican society for microbiologyAntimicrobial agents and chemotherapyAntimicrobial agents and chemotherapy, 1098-6596, Vol. 63, Nro. 2, 2019, p. e01531-18https://aac.asm.org/content/63/2/e01531-18.abstractMutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. Oralisarticleartículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Streptococcus mitiCardiolipinasDaptomicinaCdsAPgsADaptomycin resistanceWe investigated the ability of several recent clinical viridans group streptococci (VGS) bloodstream isolates (Streptococcus mitis/S. oralis subgroup) from daptomycin (DAP)-naive patients to develop DAP resistance in vitro. All strains rapidly developed high-level and stable DAP resistance. Substitutions in two enzymes involved in the cardiolipin biosynthesis pathway were identified, i.e., CdsA (phosphatidate cytidylyltransferase) and PgsA (CDP-diacylglycerol-glycerol-3-phosphate-3phosphatidyltransferase). These mutations were associated with complete disappearance of phosphatidylglycerol and cardiolipin from cell membranes. DAP interactions with the cell membrane differed in isolates with PgsA versus CdsA substitutionsAcceso cerradohttp://purl.org/coar/access_right/c_abf3282019http://purl.org/coar/access_right/c_abf2LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/602c9075-23ef-491d-ba34-ec4cca9d1605/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILTran T.T., Mishra N.N., Seepersaud R., Diaz L., Rios R., Dinh A.Q., Garcia-de-la-Maria C., Rybak M.J., Miro J.M., Shelburne S.A., Sullam P.M., Bayer A.S., Arias C.A._2019.pdf.jpgTran T.T., Mishra N.N., Seepersaud R., Diaz L., Rios R., Dinh A.Q., Garcia-de-la-Maria C., Rybak M.J., Miro J.M., Shelburne S.A., Sullam P.M., Bayer A.S., Arias C.A._2019.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/00cb14a1-d8fb-41db-857d-8af6f6dfa279/download7210a811635d1799e7c05fee5d259be7MD53ORIGINALTran T.T., Mishra N.N., Seepersaud R., Diaz L., Rios R., Dinh A.Q., Garcia-de-la-Maria C., Rybak M.J., Miro J.M., Shelburne S.A., Sullam P.M., Bayer A.S., Arias C.A._2019.pdfTran T.T., Mishra N.N., Seepersaud R., Diaz L., Rios R., Dinh A.Q., Garcia-de-la-Maria C., Rybak M.J., Miro J.M., Shelburne S.A., Sullam P.M., Bayer A.S., Arias C.A._2019.pdfapplication/pdf851104https://repositorio.unbosque.edu.co/bitstreams/9fdae48d-77ed-4c56-a821-821768cb6ac8/download211eec4c4566cb9d0ddc2e71a7cf5369MD51TEXTTran T.T., Mishra N.N., Seepersaud R., Diaz L., Rios R., Dinh A.Q., Garcia-de-la-Maria C., Rybak M.J., Miro J.M., Shelburne S.A., Sullam P.M., Bayer A.S., Arias C.A._2019.pdf.txtTran T.T., Mishra N.N., Seepersaud R., Diaz L., Rios R., Dinh A.Q., Garcia-de-la-Maria C., Rybak M.J., Miro J.M., Shelburne S.A., Sullam P.M., Bayer A.S., Arias C.A._2019.pdf.txtExtracted texttext/plain26968https://repositorio.unbosque.edu.co/bitstreams/ecb2813e-5238-4569-9955-18df3be0be9a/download53eb7dd0223dd1e075227ff68d10ff4bMD5420.500.12495/2170oai:repositorio.unbosque.edu.co:20.500.12495/21702024-02-07 12:16:52.384restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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

Publicaciones similares