Elucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tn

ilustraciones, fotografías, graficas

Autores:
Espinosa Velandia, Jessica Natalia
Tipo de recurso:
Fecha de publicación:
2022
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/82105
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/82105
https://repositorio.unal.edu.co/
Palabra clave:
570 - Biología::572 - Bioquímica
Lectinas
lectins
ANTIGENOS DE TUMORES VIRALES
Antigens viral tumor
Lectina vegetal
Salvia bogotensis
Familia Lamiaceae
Glicotopes T y Tn
Lectinas tipo L.
Vegetal lectin
Lamiaceae family
T and Tn glycotope
L-type lectins
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_ffb25902ceea933e9812942948523f29
oai_identifier_str oai:repositorio.unal.edu.co:unal/82105
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Elucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tn
dc.title.translated.eng.fl_str_mv Elucidation of the primary structure of the Salvia bogotensis lectin (LSBo-I) specific for the detection of T and Tn antigen
title Elucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tn
spellingShingle Elucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tn
570 - Biología::572 - Bioquímica
Lectinas
lectins
ANTIGENOS DE TUMORES VIRALES
Antigens viral tumor
Lectina vegetal
Salvia bogotensis
Familia Lamiaceae
Glicotopes T y Tn
Lectinas tipo L.
Vegetal lectin
Lamiaceae family
T and Tn glycotope
L-type lectins
title_short Elucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tn
title_full Elucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tn
title_fullStr Elucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tn
title_full_unstemmed Elucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tn
title_sort Elucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tn
dc.creator.fl_str_mv Espinosa Velandia, Jessica Natalia
dc.contributor.advisor.none.fl_str_mv Vega Castro, Nohora Angélica
dc.contributor.author.none.fl_str_mv Espinosa Velandia, Jessica Natalia
dc.contributor.researchgroup.spa.fl_str_mv Grupo de Investigación en Proteínas GRIP
dc.subject.ddc.spa.fl_str_mv 570 - Biología::572 - Bioquímica
topic 570 - Biología::572 - Bioquímica
Lectinas
lectins
ANTIGENOS DE TUMORES VIRALES
Antigens viral tumor
Lectina vegetal
Salvia bogotensis
Familia Lamiaceae
Glicotopes T y Tn
Lectinas tipo L.
Vegetal lectin
Lamiaceae family
T and Tn glycotope
L-type lectins
dc.subject.agrovoc.spa.fl_str_mv Lectinas
dc.subject.agrovoc.eng.fl_str_mv lectins
dc.subject.lemb.spa.fl_str_mv ANTIGENOS DE TUMORES VIRALES
dc.subject.lemb.eng.fl_str_mv Antigens viral tumor
dc.subject.proposal.spa.fl_str_mv Lectina vegetal
Salvia bogotensis
Familia Lamiaceae
Glicotopes T y Tn
Lectinas tipo L.
dc.subject.proposal.eng.fl_str_mv Vegetal lectin
Lamiaceae family
T and Tn glycotope
L-type lectins
description ilustraciones, fotografías, graficas
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-08-25T16:46:45Z
dc.date.available.none.fl_str_mv 2022-08-25T16:46:45Z
dc.date.issued.none.fl_str_mv 2022
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/82105
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/82105
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.indexed.spa.fl_str_mv RedCol
LaReferencia
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dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
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dc.format.extent.spa.fl_str_mv xix, 126 páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv Bogotá - Ciencias - Maestría en Ciencias - Bioquímica
dc.publisher.department.spa.fl_str_mv Departamento de Química
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
institution Universidad Nacional de Colombia
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https://repositorio.unal.edu.co/bitstream/unal/82105/4/1053336051.2022.pdf
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Vega Castro, Nohora Angélica1bac60b0381f939db8d6c334f123ddc1Espinosa Velandia, Jessica Natalia7f8c1f7c5fef78a4c149be581eebb77cGrupo de Investigación en Proteínas GRIP2022-08-25T16:46:45Z2022-08-25T16:46:45Z2022https://repositorio.unal.edu.co/handle/unal/82105Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, graficasEl estudio de las lectinas vegetales ha sido de gran importancia debido a su capacidad de reconocer residuos de carbohidratos, los cuales han sido llamados glicotopes; alguno de ellos se encuentra enmascarados en la superficie celular y están asociados con una gran variedad de carcinomas. Algunas de las lectinas que más se han estudiado son las de origen vegetal de la familia Leguminosae, sin embargo, también se han aislado de otras familias botánicas, tales como Lamiácea. Las lectinas de Lamiáceas se caracterizan porque reconocen los glicotopes T y/o Tn. La información disponible acerca de estas proteínas es muy escasa debido a que los procesos de purificación no permiten obtener la cantidad necesaria para realizar estudios de caracterización estructural y de interacción con el antígeno Tn/T; por otra parte, hasta el momento solo hay dos secuencias de especies relacionadas obtenidas a partir del cDNA, la lectina de Salvia miltorriza (LSM) y de Gleheda y solo se han caracterizado cuatro lectinas de la zona de Eurasia y dos lectinas de especies endémicas de Colombia, la lectina de Salvia bogotensis (LSBo-I) y de Lepechinia bullata (LLb-I) En el presente trabajo, se realizó la purificación de la lectina de Salvia bogotensis I (LSBo-I), empleando cromatografía de intercambio iónico y afinidad. Las fracciones obtenidas presentaron actividad aglutinante por eritrocitos Tn y se observó por SDS PAGE, una banda alrededor de 36 kDa por SDS-PAGE que es similar a lo obtenido en estudios previos. La sepharosa 4B, fue acoplada a una IgG de conejo (antiLSBo-I), y la elución se llevó a cabo mediante un gradiente de pH. En cada etapa de purificación se evaluó la actividad aglutinante con eritrocitos humanos A+, los cuales fueron tratados enzimáticamente con neuraminidasa y B- galactosidasa para exponer el Tn. Se obtuvo el 71% de la secuencia y por predicción se determinó que pertenece a las lectinas tipo Leguminosa (L). También se llevaron a cabo ensayos para la obtención del ADNc y la secuencia de nucleótidos de la LSBo-I. (Texto tomado de la fuente)Plant lectins studies have been of great importance due to their ability to recognize carbohydrate residues on surface cells, epitopes-like glycotopes are called. Some of them are masked on the cell surface and are associated with a wide variety of carcinomas. The most studied lectins belong to the Leguminosae family however, they have also been isolated from other botanical families, such as Lamiaceae. Their lectins are characterized by recognizing T and/or Tn glycotypes. The information available about these proteins is very scarce owing to purification processes do not allow obtaining the amount to carry out structural studies and interaction with Tn/T glycans. Secondly, there are only two sequences related to Lamiacea lectins, Salvia miltorriza (SML) and Gleheda and four lectins have been studied from Eurasia zone and two endemic lectins from Colombia, Salvia bogotensis (SBoL-I) and Lepechinia bullata (LBL-I). In this work, Ion-exchange and affinity chromatography’s led to getting SBoL-I that showed only a band by SDS PAGE, around 36 kDa, according to previous studies. Main purification step was carried out using affinity chromatography, a Sepharose 4B was coupled to IgG (antiSBoL-I) and pH gradient elution. The agglutinating activity was tested using A+ human erythrocytes, which were enzymatically treated with neuraminidase and B−galactosidase, to expose Tn structures. Finally, a sequence with 71% coverage was obtained and three-dimensional model type of Leguminous was predicted by homology. Additionally, cDNA assays were carried out to get nucleotide sequence from SBoL-I.MaestríaMagíster en Ciencias - BioquímicaLectinas de Lamiaceaxix, 126 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BioquímicaDepartamento de QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - BioquímicaLectinaslectinsANTIGENOS DE TUMORES VIRALESAntigens viral tumorLectina vegetalSalvia bogotensisFamilia LamiaceaeGlicotopes T y TnLectinas tipo L.Vegetal lectinLamiaceae familyT and Tn glycotopeL-type lectinsElucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y TnElucidation of the primary structure of the Salvia bogotensis lectin (LSBo-I) specific for the detection of T and Tn antigenTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMRedColLaReferenciaPérez G, Vega N. 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Genomics. 2011;98(4):272–9.EstudiantesInvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unal.edu.co/bitstream/unal/82105/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINAL1053336051.2022.pdf1053336051.2022.pdfTesis de Maestría en Bioquímicaapplication/pdf4514690https://repositorio.unal.edu.co/bitstream/unal/82105/4/1053336051.2022.pdff9ee704978c975331ded3e8a4b10caf3MD54unal/82105oai:repositorio.unal.edu.co:unal/821052023-01-23 17:08:35.901Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.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