Compuestos orgánicos volátiles de Trichoderma spp. con actividad biocontroladora sobre el patógeno Fusarium oxysporum f. sp. lycopersici y el insecto plaga Trialeurodes vaporariorum en plantas de tomate

The effect of the volatile organic compounds (VOCs) emitted by 15 strains of Trichoderma spp. was evaluated on (1) in vitro radial growth of Fusarium oxysporum f. sp. lycopersici (FoL59), (2) the severity of the disease in planta and (3) the oviposition rate of the greenhouse whitefly Trialeurodes vaporariorum. For the in vitro experiment, we used the double plate technique, where two Petri plates, one with Trichoderma spp. and other with F. oxysporum grown on PDA were placed one over the other for ten days at 25°C, the inhibition rate was registered. Seven strains showed the best inhibition rates of the pathogen: Th004 (46.1%), M44 (45,8%), Th035(40,5%), Th019(39,8%), Th007 (39,4%), 3T (39%) and M45 (37.1%). These strains were assessed in planta by exposing the roots of tomato seedling to the VOCs emitted by Trichoderma spp. in a sealed environment for 21 days. The progress and severity of the disease were assessed. Only the strain Th035 showed a significant difference compared to control plants, reducing by 31% the severity of the disease. Finally, the oviposition preference index (OPI) of the whitefly on VOCs treated tomato plants. Negative OPI values were observed with the strains Th035 (-27.30), M44 (-21.80), Th007 (-17.17) and M45(-7,46), while Th004, 3T, and Th019 showed a stimulant effect on the oviposition. These findings demonstrate a biocontrol activity generated by the fungal VOCs of Trichoderma spp. against two different tomato plant health problems and contribute to the development of biological control strategies via bioprospection of fungal metabolites.

• Autores: Ramírez Alarcón, Sandra Milena
• Tipo de recurso: Work document
• Fecha de publicación: 2019
• Institución: Universidad Nacional de Colombia
• Repositorio: Universidad Nacional de Colombia
• Idioma: spa
• OAI Identifier: oai:repositorio.unal.edu.co:unal/77894
• Acceso en línea: https://repositorio.unal.edu.co/handle/unal/77894
• Palabra clave: 632 - Lesiones, enfermedades, plagas vegetales; 633 - Cultivos de campo y de plantación; 668 - Tecnología de otros productos orgánicos; 333 - Economía de la tierra y de la energía; bioprospección; marchitez vascular; índice de preferencia de oviposición; control biológico; inhibición de crecimiento radial; Radial growth inhibition; bioprospecting; vascular wilt; oviposition preference index; biological control; radial growth inhibition
• Rights: openAccess
• License: Atribución-NoComercial 4.0 Internacional