El receptor tipo quinasa COK-4 de Phaseolus vulgaris L. es requerido para la activación temprana de mecanismos de defensa contra la raza 7 de Colletotrichum lindemuthianum
ilustraciones, fotografías, gráficas, tablas
- Autores:
-
Pedroza Padilla, Marlene Carolina
- Tipo de recurso:
- Fecha de publicación:
- 2019
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/76074
- Palabra clave:
- 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
Hongos patógenos
Phaseolus vulgaris
Colletotrichum lindemuthianum
Quinasas
pathogenic fungi
Phaseolus vulgaris
Colletotrichum lindemuthianum
kinases
SAS13
COK-4
Resistencia
Peróxido de hidrógeno
PTI
PRs
Ácido salicílico.
SAS13
COK-4
Resistance
Hydrogen peroxide
PTI
PRs
Salicylic acid
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
El receptor tipo quinasa COK-4 de Phaseolus vulgaris L. es requerido para la activación temprana de mecanismos de defensa contra la raza 7 de Colletotrichum lindemuthianum |
| dc.title.alternative.spa.fl_str_mv |
The COK-4 kinase receptor of Phaseolus vulgaris L. is required for the early activation of defense mechanisms against race 7 of Colletotrichum lindemuthianum |
| title |
El receptor tipo quinasa COK-4 de Phaseolus vulgaris L. es requerido para la activación temprana de mecanismos de defensa contra la raza 7 de Colletotrichum lindemuthianum |
| spellingShingle |
El receptor tipo quinasa COK-4 de Phaseolus vulgaris L. es requerido para la activación temprana de mecanismos de defensa contra la raza 7 de Colletotrichum lindemuthianum 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales Hongos patógenos Phaseolus vulgaris Colletotrichum lindemuthianum Quinasas pathogenic fungi Phaseolus vulgaris Colletotrichum lindemuthianum kinases SAS13 COK-4 Resistencia Peróxido de hidrógeno PTI PRs Ácido salicílico. SAS13 COK-4 Resistance Hydrogen peroxide PTI PRs Salicylic acid |
| title_short |
El receptor tipo quinasa COK-4 de Phaseolus vulgaris L. es requerido para la activación temprana de mecanismos de defensa contra la raza 7 de Colletotrichum lindemuthianum |
| title_full |
El receptor tipo quinasa COK-4 de Phaseolus vulgaris L. es requerido para la activación temprana de mecanismos de defensa contra la raza 7 de Colletotrichum lindemuthianum |
| title_fullStr |
El receptor tipo quinasa COK-4 de Phaseolus vulgaris L. es requerido para la activación temprana de mecanismos de defensa contra la raza 7 de Colletotrichum lindemuthianum |
| title_full_unstemmed |
El receptor tipo quinasa COK-4 de Phaseolus vulgaris L. es requerido para la activación temprana de mecanismos de defensa contra la raza 7 de Colletotrichum lindemuthianum |
| title_sort |
El receptor tipo quinasa COK-4 de Phaseolus vulgaris L. es requerido para la activación temprana de mecanismos de defensa contra la raza 7 de Colletotrichum lindemuthianum |
| dc.creator.fl_str_mv |
Pedroza Padilla, Marlene Carolina |
| dc.contributor.advisor.spa.fl_str_mv |
Gonzalez Almario, Adriana Rincón Rivera, Linda Jeimmy |
| dc.contributor.author.spa.fl_str_mv |
Pedroza Padilla, Marlene Carolina |
| dc.subject.ddc.spa.fl_str_mv |
630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales |
| topic |
630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales Hongos patógenos Phaseolus vulgaris Colletotrichum lindemuthianum Quinasas pathogenic fungi Phaseolus vulgaris Colletotrichum lindemuthianum kinases SAS13 COK-4 Resistencia Peróxido de hidrógeno PTI PRs Ácido salicílico. SAS13 COK-4 Resistance Hydrogen peroxide PTI PRs Salicylic acid |
| dc.subject.agrovoc.spa.fl_str_mv |
Hongos patógenos Phaseolus vulgaris Colletotrichum lindemuthianum Quinasas |
| dc.subject.agrovoc.eng.fl_str_mv |
pathogenic fungi Phaseolus vulgaris Colletotrichum lindemuthianum kinases |
| dc.subject.proposal.spa.fl_str_mv |
SAS13 COK-4 Resistencia Peróxido de hidrógeno PTI PRs Ácido salicílico. |
| dc.subject.proposal.eng.fl_str_mv |
SAS13 COK-4 Resistance Hydrogen peroxide PTI PRs Salicylic acid |
| description |
ilustraciones, fotografías, gráficas, tablas |
| publishDate |
2019 |
| dc.date.issued.spa.fl_str_mv |
2019 |
| dc.date.accessioned.spa.fl_str_mv |
2020-03-13T16:42:00Z |
| dc.date.available.spa.fl_str_mv |
2020-03-13T16:42:00Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/76074 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.none.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/76074 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Theor Appl Genet 109: 690-699. doi:10.1007/s00122-004-1697-6. Meziadi C., Richard M., Derquennes A., Thareau V., Blanchet S., Gratias A., Pflieger S., Geffroy V. (2016) Development of molecular markers linked to disease resistance genes in common bean based on whole genome sequence. Plant Sci. 242: 351-357. doi: 10.1016/j.plantsci.2015.09.006. Miklas, P., Johnson, E., Stone, V., Beaver, J., Montoya, C., Zapata, M. (1996) Selective mapping of QTL conditioning disease resistance in common bean. Crop Sci. 36: 1344-1351. doi:10.2135/cropsci1996.0011183X003600050044x. Miklas, P., J. Kelly (2002) The use of MAS to develop into bean germplasm possessing Co-42 gene for anthracnose resistance. Annu. Rep. Bean Improvement Coop. 45: 68-69. Miklas, P.N., Kelly, J.D., Beebe, S.D., Blair, M.W. (2006). Common bean breeding for resistance against biotic and abiotic stresses: From classical to MAS breeding. Euphytica 147: 105–131. Nissen, K., Willats, W., Malinovsky, F. 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(2019) Deleción en el gen de resistencia Co-42 de Phaseolus vulgaris L. afecta la activación temprana de defensa contra Colletotrichum lindemuthianum REV. Fitopatología Colombiana, memorias. 43 (2): 181. Pereira, M., Araújo, C., Araújo, E., Vieira, M., Soares, D. (2013) Beginning to Understand the Role of Sugar Carriers in Colletotrichum lindemuthianum: the Function of the Gene mfs1. J Microbiol. 51 (1): 70–81. doi: 10.1007/s12275-013-2393-5. Potters, G., Horemans, N., Jansen, M.A. (2010) The cellular redox state in plant stress biology – A charging concept. Plant Physiol Biochem. 48 (5): 292-300. doi:10.1016/j.plaphy.2009.12.007. Riascos, J. (2001). Caracterización de la diversidad genética de Colletotrichum lindemuthianum usando marcadores moleculares. Trabajo de grado. Universidad del Valle. Facultad de Ciencias. Cali, Colombia. 70 p. Rincón, L.J. (2007). Diversidad genética de Colletotrichum lindemuthianum que afecta frijol en las zonas productoras de los departamentos de Cundinamarca, Boyacá y Santander. Trabajo de grado M.Sc. en Ciencias Agrarias. Universidad Nacional de Colombia. Facultad de Agronomía. 90p. Rodrigues, P., Borges, A., Chowdhury, B., Gomes, D., Mui, S., Aranha, L., Melotto M. (2012) Dissecting Phaseolus Vulgaris Innate Immune System against Colletotrichum lindemuthianum. Plos One 7(8): 1-14. doi:10.1371/journal.pone.0043161. Rodrigues, P., Francisco, C., Melotto, M. (2015). The Co‑4 locus on chromosome Pv08 contains a unique cluster of 18 COK‑4 genes and is regulated by immune response in common bean. Theor Appl Genet. 128 (6): 1193-208. doi:10.1007/s00122-015-2500-6. Saxena, I., Srikanth, S., Chen, Z. (2016) Cross Talk between H2O2 and Interacting Signal Molecules under Plant Stress Response. Front. Plant Sci. 7:570. doi: 10.3389/fpls.2016.00570. Schoonhoven, A., Pastor Corrales, M. (eds.) 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(1998) Marker assisted dissection of the oligogenic anthracnose resistance in common bean cultivar, G2333. Theor Appl Genet 96: 87–94. doi:10.1007/s001220050. Zavaliev, R., Epel, B. L. (2014). Imaging Callose at Plasmodesmata Using Aniline Blue: Quantitative Confocal Microscopy. Plasmodesmata, 105–119. doi:10.1007/978-1-4939-1523-1_7. |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gonzalez Almario, Adriana2095714a2ee0a49a87d2319bc2e287b4Rincón Rivera, Linda Jeimmya83f15f0-b2fb-48b1-9f01-ffd43b6884bfPedroza Padilla, Marlene Carolinae4600173-f53e-491d-b4d2-8d4d52cbacb52020-03-13T16:42:00Z2020-03-13T16:42:00Z2019https://repositorio.unal.edu.co/handle/unal/76074Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, gráficas, tablasColletotrichum lindemuthianum (Sacc. & Magnus) es un hongo fitopatógeno que provoca la antracnosis, enfermedad limitante que se manifiesta con el desarrollo de lesiones necróticas en vainas, hojas, peciolo y tallos, afectando la calidad de semillas y productos comerciables de fríjol común (Phaseolus vulgaris L.). Dentro de las medidas de manejo se halla el uso de cultivares de frijol, obtenidos de programas de mejoramiento a los cuales se les han introgresado los loci Co-42 y Co-5, conocidos por conferir resistencia a varias razas de C. lindemuthianum. Sin embargo, el papel de estos genes en la activación temprana de la inmunidad vegetal en cultivares como Serranía y Sutagao, no se había evaluado. Por tal motivo, se plantearon como objetivos relacionar la presencia de los genes Co con cambios en la activación y en el tiempo de respuesta de algunos mecanismos de defensa en presencia de la raza 7 del patógeno, a través del uso de marcadores moleculares, pruebas histoquímicas y expresión diferencial de genes. Entre los resultados, se pudo comprobar que Co-42 y Co-5 están presentes en el cultivar Serranía, mientras que en Sutagao solo Co-42 el cual presentó una deleción en el gen COK-4 que codifica para un receptor tipo quinasa (RLK). En ensayos de patogenicidad se determinó que el frijol Sutagao fue susceptible al hongo, en contraste a Serranía que fue resistente. Los ensayos histoquímicos con azul de anilina y 3,3´-diaminobencidina, revelaron que el frijol Serranía indujo tempranamente mayores deposiciones de calosa y especies reactivas de oxígeno en los sitios de intento de penetración del patógeno, lo cual también fue favorecido por la expresión de genes relacionados con defensa como PR-1 y peroxidasas, en contraste al cultivar Sutagao que activó tardíamente dichos mecanismos. Esto indica que, el receptor COK-4 y el locus Co-5 podrían estar participando en la inducción temprana de mecanismos de defensa durante la interacción P. vulgaris L. – C. lindemuthianum. (Texto tomado de la fuente).Colletotrichum lindemuthianum (Sacc. & Magnus) is a phytopathogenic fungus that causes anthracnose, a limiting disease characterized by the presence of necrotic lesions in pods, leaves, petiole and stems, affecting the quality of seeds and commercial common bean products (Phaseolus vulgaris L.). Among the management measures is the use of bean cultivars that have Co-42 and Co-5 loci, transfered by breeding programs and confer resistance to several races of C. lindemuthianum. However, the role of these genes in the early activation of plant immunity in cultivars such as Serranía and Sutagao, has not been evaluated. For this reason, the objectives were to relate the presence of Co genes with changes in the activation and response time of some defense mechanisms in the presence of race 7 of the pathogen, through the use of molecular markers, histochemical tests and differential gene expression. Among the results, it was found that Co-42 and Co-5 are present in Serrania cultivar, while in Sutagao a deletion was found in Co-42, specifically in the COK-4 gene that codes for a Receptor-like Kinase (RLK). In pathogenicity tests Sutagao was susceptible to the fungus, in contrast to Serranía that was resistant. Histochemical tests with aniline blue and 3,3'-diaminobenzidine, revealed that Serrania early induced more depositions of callose and reactive oxygen species at sites of attempted penetration of pathogen, which was also favored by the expression of defense-related genes such as PR-1 and peroxidases, in contrast to Sutagao that delayed such mechanisms. This indicates that, the COK-4 receptor and locus Co-5 are required for the early induction of defense mechanisms during the P. vulgaris L. - C. lindemuthianum interaction.División de Investigación de la sede Bogotá (DIB)Determinación de genes Co en las variedades mejoradas de Phaseolus vulgarisL.Sutagao y Serranía y su importancia en la activación de respuesta de defensa contra Colletotrichumlindemuthianum.Magíster en Ciencias Agrarias. Línea de Investigación: Fitopatología.MaestríaMagíster en Ciencias AgrariasFitopatologíaCiencias Agronómicasxx, 114 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias Agrarias - Maestría en Ciencias AgrariasEscuela de posgradosFacultad de Ciencias AgrariasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetalesHongos patógenosPhaseolus vulgarisColletotrichum lindemuthianumQuinasaspathogenic fungiPhaseolus vulgarisColletotrichum lindemuthianumkinasesSAS13COK-4ResistenciaPeróxido de hidrógenoPTIPRsÁcido salicílico.SAS13COK-4ResistanceHydrogen peroxidePTIPRsSalicylic acidEl receptor tipo quinasa COK-4 de Phaseolus vulgaris L. es requerido para la activación temprana de mecanismos de defensa contra la raza 7 de Colletotrichum lindemuthianumThe COK-4 kinase receptor of Phaseolus vulgaris L. is required for the early activation of defense mechanisms against race 7 of Colletotrichum lindemuthianumTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAfzal, A., Wood, A., Lightfoot, D. 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