Diversidad genética de bananos y bananitos con microsatélites fluorescentes

Banana (Musa spp.) its fundamental to the economy of developing countries, including our country. Because of this reasons, the characterization of musaceas genetic diversity is essential to the management and exploitation of its genetic resources. In the current study 99 accessions from the collecti...

Full description

Autores:
Gutierrez Salamanca, Madeleine Lieset
Tipo de recurso:
Informe
Fecha de publicación:
2017
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/78310
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/78310
Palabra clave:
Diversidad genética
marcadores moleculares
Musáceas
simple sequence repeats
SSR
Genetic diversity
molecular markers
Musaceas
simple sequence repeats
SSR
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
Description
Summary:Banana (Musa spp.) its fundamental to the economy of developing countries, including our country. Because of this reasons, the characterization of musaceas genetic diversity is essential to the management and exploitation of its genetic resources. In the current study 99 accessions from the collection of Musa spp. that are part of the germplasm bank of the Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA, Palmira, Valle del Cauca), were evaluated by twelve fluorescent microsatellite molecular markers (SSR). A total of 206 alleles were identified, with a polymorphic information content (PIC) average of 0.106 and a marker index (IM) average of 1.377, indicating the presence of polymorphic and informative markers. The expected heterozygocity and number of alleles were superior in banana and ornamentals (He=0.836–Na=14.1 y He=0.848–Na=8.5, respectively), while bananitos presented inferior values (He=0.569–Na=6.25). The dissimilarity analysis allowed to identify possible duplicate accessions, given its identical genetic profile as: NATU08, NATU09, SABO03 y SABO01. Cluster and structure analysis identified three highly differentiated population groups, one formatted by bananitos, and the other two by banana of commercial cultivars and banana with wild characteristics plus ornamental ones. In conclusion, the collection presents a high genetic diversity mainly in the banana and ornamentals and in smaller amounts in bananitos; likewise, it is divided into discrete populations with high identity and low gene flow.