Comparison of dna extraction methods for detection of citrus huanglongbing in colombia
Four DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several c...
- Autores:
-
Angel, Jorge Evelio
Hernández, Erick Geovanni
Herrera, Néstor Andrés
Gómez, Linda Yhiset
Castro, Ángela Patricia
Sepúlveda, Adriana Milena
Ebratt, Everth Emilio
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2014
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/73652
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/73652
http://bdigital.unal.edu.co/38128/
- Palabra clave:
- Biologia molecular
Agronomia
Candidatus Liberibacter
Diaphorina citri
Psyllidae
vectors.
Fitopatología
entomología
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Angel, Jorge Evelio66c58455-5a7e-47e2-a2dc-ec333110ccfa300Hernández, Erick Geovanni1292b4a5-0490-48d0-9dad-a762ae006a6f300Herrera, Néstor Andrés46c01ca4-e92d-4946-9913-a4ae01ba9a23300Gómez, Linda Yhiset9f50d004-e6de-4ed9-86c7-2bd493bbf141300Castro, Ángela Patricia0fb04941-c159-4a8b-8f19-b1659405b788300Sepúlveda, Adriana Milena74628800-2cd3-450e-9bef-73ae1e55199e300Ebratt, Everth Emilio6db70a5c-ca7a-4e4f-aff7-fafcc9ab7d123002019-07-03T16:39:09Z2019-07-03T16:39:09Z2014-03-26https://repositorio.unal.edu.co/handle/unal/73652http://bdigital.unal.edu.co/38128/Four DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several criterias such as integrity, purity and concentration. The best quality parameters presented in terms of extraction of DNA from plant midribs tissue of citrus, were cited by Murray and Thompson (1980) and Rodríguez et al. (2010), while for the DNA extraction from psyllid vectors of HLB, the best extraction method was suggested by Manjunath et al. (2008).application/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/40544Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaAgronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 2357-3732 0120-9965Angel, Jorge Evelio and Hernández, Erick Geovanni and Herrera, Néstor Andrés and Gómez, Linda Yhiset and Castro, Ángela Patricia and Sepúlveda, Adriana Milena and Ebratt, Everth Emilio (2014) Comparison of dna extraction methods for detection of citrus huanglongbing in colombia. Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 2357-3732 0120-9965 .Comparison of dna extraction methods for detection of citrus huanglongbing in colombiaArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTBiologia molecularAgronomiaCandidatus LiberibacterDiaphorina citriPsyllidaevectors.FitopatologíaentomologíaORIGINAL40544-182608-1-SP.pptapplication/vnd.ms-powerpoint128000https://repositorio.unal.edu.co/bitstream/unal/73652/1/40544-182608-1-SP.pptae4e1dd7f9786d6582e3b9d83db13b18MD5140544-182610-1-SP.xlsapplication/vnd.ms-excel142848https://repositorio.unal.edu.co/bitstream/unal/73652/2/40544-182610-1-SP.xls191cb5042ce202abf716f9b0e2813abfMD5240544-203481-1-PB.epubapplication/epub+zip602423https://repositorio.unal.edu.co/bitstream/unal/73652/3/40544-203481-1-PB.epub689a4e985fc7a7931eade4a254e9879dMD5340544-182607-1-SP.docapplication/msword349184https://repositorio.unal.edu.co/bitstream/unal/73652/4/40544-182607-1-SP.doc68227cc74501c3a9ebc7676dca139efeMD5440544-201996-1-PB.pdfapplication/pdf192048https://repositorio.unal.edu.co/bitstream/unal/73652/5/40544-201996-1-PB.pdf02e8a081d65282be6f4b7702bd361971MD55THUMBNAIL40544-201996-1-PB.pdf.jpg40544-201996-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg9271https://repositorio.unal.edu.co/bitstream/unal/73652/6/40544-201996-1-PB.pdf.jpg3360974a92a23510ded398f56ac57681MD56unal/73652oai:repositorio.unal.edu.co:unal/736522023-06-29 23:04:12.257Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
| dc.title.spa.fl_str_mv |
Comparison of dna extraction methods for detection of citrus huanglongbing in colombia |
| title |
Comparison of dna extraction methods for detection of citrus huanglongbing in colombia |
| spellingShingle |
Comparison of dna extraction methods for detection of citrus huanglongbing in colombia Biologia molecular Agronomia Candidatus Liberibacter Diaphorina citri Psyllidae vectors. Fitopatología entomología |
| title_short |
Comparison of dna extraction methods for detection of citrus huanglongbing in colombia |
| title_full |
Comparison of dna extraction methods for detection of citrus huanglongbing in colombia |
| title_fullStr |
Comparison of dna extraction methods for detection of citrus huanglongbing in colombia |
| title_full_unstemmed |
Comparison of dna extraction methods for detection of citrus huanglongbing in colombia |
| title_sort |
Comparison of dna extraction methods for detection of citrus huanglongbing in colombia |
| dc.creator.fl_str_mv |
Angel, Jorge Evelio Hernández, Erick Geovanni Herrera, Néstor Andrés Gómez, Linda Yhiset Castro, Ángela Patricia Sepúlveda, Adriana Milena Ebratt, Everth Emilio |
| dc.contributor.author.spa.fl_str_mv |
Angel, Jorge Evelio Hernández, Erick Geovanni Herrera, Néstor Andrés Gómez, Linda Yhiset Castro, Ángela Patricia Sepúlveda, Adriana Milena Ebratt, Everth Emilio |
| dc.subject.proposal.spa.fl_str_mv |
Biologia molecular Agronomia Candidatus Liberibacter Diaphorina citri Psyllidae vectors. Fitopatología entomología |
| topic |
Biologia molecular Agronomia Candidatus Liberibacter Diaphorina citri Psyllidae vectors. Fitopatología entomología |
| description |
Four DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several criterias such as integrity, purity and concentration. The best quality parameters presented in terms of extraction of DNA from plant midribs tissue of citrus, were cited by Murray and Thompson (1980) and Rodríguez et al. (2010), while for the DNA extraction from psyllid vectors of HLB, the best extraction method was suggested by Manjunath et al. (2008). |
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2014 |
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2014-03-26 |
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2019-07-03T16:39:09Z |
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2019-07-03T16:39:09Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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Text |
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publishedVersion |
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https://repositorio.unal.edu.co/handle/unal/73652 |
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http://bdigital.unal.edu.co/38128/ |
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https://repositorio.unal.edu.co/handle/unal/73652 http://bdigital.unal.edu.co/38128/ |
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spa |
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http://revistas.unal.edu.co/index.php/agrocol/article/view/40544 |
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Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana Agronomía Colombiana |
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Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 2357-3732 0120-9965 |
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Angel, Jorge Evelio and Hernández, Erick Geovanni and Herrera, Néstor Andrés and Gómez, Linda Yhiset and Castro, Ángela Patricia and Sepúlveda, Adriana Milena and Ebratt, Everth Emilio (2014) Comparison of dna extraction methods for detection of citrus huanglongbing in colombia. Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 2357-3732 0120-9965 . |
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Derechos reservados - Universidad Nacional de Colombia |
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