Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest

Hydrolytic activity was evaluated in a mixture of supernatants produced by filamentous fungi grown individually on microcrystalline cellulose and empty fruit bunches. The strains that were used correspond to two types of isolates; the first was made from soil samples from a transect of a high andean...

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Autores:
Moya Alvarez, Luz Aida
Torres R., Esperanza
Tipo de recurso:
Article of journal
Fecha de publicación:
2012
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/40779
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/40779
http://bdigital.unal.edu.co/30876/
Palabra clave:
consortia
enzymatic hydrolysis
empty fruit bunches
cellulases
filamentous fungi
biomass.
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openAccess
License
Atribución-NoComercial 4.0 Internacional
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network_acronym_str UNACIONAL2
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repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Moya Alvarez, Luz Aidaf9f1b533-9f02-483f-b084-f73ca27fa42a300Torres R., Esperanza02d2938a-c663-4e04-891c-42213a8f9ab53002019-06-28T09:40:37Z2019-06-28T09:40:37Z2012https://repositorio.unal.edu.co/handle/unal/40779http://bdigital.unal.edu.co/30876/Hydrolytic activity was evaluated in a mixture of supernatants produced by filamentous fungi grown individually on microcrystalline cellulose and empty fruit bunches. The strains that were used correspond to two types of isolates; the first was made from soil samples from a transect of a high andean forest of Colombia, in the Parque Natural Nacional de Los Nevados, where, based on previous studies, we selected the strains B7, B11, B11M and B19. The second isolate was obtained from a pool of oil palm empty fruit bunches (Eleaeis guinensis Jacq.) in different states of decomposition on the Unipalma plantation located in the eastern plains; strains TA1 and TA2. To perform the hydrolysis of cellulose and empty fruit bunches, the previously obtained supernatants from each of the selected strains were cultivated for 300 hours in cellulose and characterized individually by endoglucanase, exoglucanase, and β-glucosidase activity. Individual supernatants were mixed at a 1:1 ratio to form consortia; and hydrolytic activity was evaluated in the substrates at two hours. The glucose concentration was determined by the 3,5-dinitrosalicylic acid (DNS) method. The results show that hydrolysis of empty fruit bunch to glucose was favored by three pools of supernatants, with increases greater than 400% in comparison with the hydrolysis obtained by individual supernatants, demonstrating the potential to decompose palm empty fruit bunches; thereby contributing to the reduction of decay time of empty fruit bunches and the decrease of environmental and health problems.application/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/29874Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaAgronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 2357-3732 0120-9965Moya Alvarez, Luz Aida and Torres R., Esperanza (2012) Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest. Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 2357-3732 0120-9965 .Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forestArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTconsortiaenzymatic hydrolysisempty fruit bunchescellulasesfilamentous fungibiomass.ORIGINAL29874-165678-2-PB.pdfapplication/pdf320256https://repositorio.unal.edu.co/bitstream/unal/40779/1/29874-165678-2-PB.pdf298a2b0b096e6ddffd799677ee0d9a96MD5129874-107982-1-SP.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheet18176https://repositorio.unal.edu.co/bitstream/unal/40779/2/29874-107982-1-SP.xlsx6ca6606da5a8baa994caab415518aa9cMD52THUMBNAIL29874-165678-2-PB.pdf.jpg29874-165678-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg9169https://repositorio.unal.edu.co/bitstream/unal/40779/3/29874-165678-2-PB.pdf.jpga1b59539fccb09d2ae3248f6efde6f8bMD53unal/40779oai:repositorio.unal.edu.co:unal/407792023-01-30 23:04:56.451Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest
title Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest
spellingShingle Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest
consortia
enzymatic hydrolysis
empty fruit bunches
cellulases
filamentous fungi
biomass.
title_short Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest
title_full Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest
title_fullStr Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest
title_full_unstemmed Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest
title_sort Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest
dc.creator.fl_str_mv Moya Alvarez, Luz Aida
Torres R., Esperanza
dc.contributor.author.spa.fl_str_mv Moya Alvarez, Luz Aida
Torres R., Esperanza
dc.subject.proposal.spa.fl_str_mv consortia
enzymatic hydrolysis
empty fruit bunches
cellulases
filamentous fungi
biomass.
topic consortia
enzymatic hydrolysis
empty fruit bunches
cellulases
filamentous fungi
biomass.
description Hydrolytic activity was evaluated in a mixture of supernatants produced by filamentous fungi grown individually on microcrystalline cellulose and empty fruit bunches. The strains that were used correspond to two types of isolates; the first was made from soil samples from a transect of a high andean forest of Colombia, in the Parque Natural Nacional de Los Nevados, where, based on previous studies, we selected the strains B7, B11, B11M and B19. The second isolate was obtained from a pool of oil palm empty fruit bunches (Eleaeis guinensis Jacq.) in different states of decomposition on the Unipalma plantation located in the eastern plains; strains TA1 and TA2. To perform the hydrolysis of cellulose and empty fruit bunches, the previously obtained supernatants from each of the selected strains were cultivated for 300 hours in cellulose and characterized individually by endoglucanase, exoglucanase, and β-glucosidase activity. Individual supernatants were mixed at a 1:1 ratio to form consortia; and hydrolytic activity was evaluated in the substrates at two hours. The glucose concentration was determined by the 3,5-dinitrosalicylic acid (DNS) method. The results show that hydrolysis of empty fruit bunch to glucose was favored by three pools of supernatants, with increases greater than 400% in comparison with the hydrolysis obtained by individual supernatants, demonstrating the potential to decompose palm empty fruit bunches; thereby contributing to the reduction of decay time of empty fruit bunches and the decrease of environmental and health problems.
publishDate 2012
dc.date.issued.spa.fl_str_mv 2012
dc.date.accessioned.spa.fl_str_mv 2019-06-28T09:40:37Z
dc.date.available.spa.fl_str_mv 2019-06-28T09:40:37Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.content.spa.fl_str_mv Text
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dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/30876/
url https://repositorio.unal.edu.co/handle/unal/40779
http://bdigital.unal.edu.co/30876/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/agrocol/article/view/29874
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
Agronomía Colombiana
dc.relation.ispartofseries.none.fl_str_mv Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 2357-3732 0120-9965
dc.relation.references.spa.fl_str_mv Moya Alvarez, Luz Aida and Torres R., Esperanza (2012) Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest. Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 2357-3732 0120-9965 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
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eu_rights_str_mv openAccess
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial
institution Universidad Nacional de Colombia
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