Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest
Hydrolytic activity was evaluated in a mixture of supernatants produced by filamentous fungi grown individually on microcrystalline cellulose and empty fruit bunches. The strains that were used correspond to two types of isolates; the first was made from soil samples from a transect of a high andean...
- Autores:
-
Moya Alvarez, Luz Aida
Torres R., Esperanza
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2012
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/40779
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/40779
http://bdigital.unal.edu.co/30876/
- Palabra clave:
- consortia
enzymatic hydrolysis
empty fruit bunches
cellulases
filamentous fungi
biomass.
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Universidad Nacional de Colombia |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Moya Alvarez, Luz Aidaf9f1b533-9f02-483f-b084-f73ca27fa42a300Torres R., Esperanza02d2938a-c663-4e04-891c-42213a8f9ab53002019-06-28T09:40:37Z2019-06-28T09:40:37Z2012https://repositorio.unal.edu.co/handle/unal/40779http://bdigital.unal.edu.co/30876/Hydrolytic activity was evaluated in a mixture of supernatants produced by filamentous fungi grown individually on microcrystalline cellulose and empty fruit bunches. The strains that were used correspond to two types of isolates; the first was made from soil samples from a transect of a high andean forest of Colombia, in the Parque Natural Nacional de Los Nevados, where, based on previous studies, we selected the strains B7, B11, B11M and B19. The second isolate was obtained from a pool of oil palm empty fruit bunches (Eleaeis guinensis Jacq.) in different states of decomposition on the Unipalma plantation located in the eastern plains; strains TA1 and TA2. To perform the hydrolysis of cellulose and empty fruit bunches, the previously obtained supernatants from each of the selected strains were cultivated for 300 hours in cellulose and characterized individually by endoglucanase, exoglucanase, and β-glucosidase activity. Individual supernatants were mixed at a 1:1 ratio to form consortia; and hydrolytic activity was evaluated in the substrates at two hours. The glucose concentration was determined by the 3,5-dinitrosalicylic acid (DNS) method. The results show that hydrolysis of empty fruit bunch to glucose was favored by three pools of supernatants, with increases greater than 400% in comparison with the hydrolysis obtained by individual supernatants, demonstrating the potential to decompose palm empty fruit bunches; thereby contributing to the reduction of decay time of empty fruit bunches and the decrease of environmental and health problems.application/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/29874Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaAgronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 2357-3732 0120-9965Moya Alvarez, Luz Aida and Torres R., Esperanza (2012) Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest. Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 2357-3732 0120-9965 .Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forestArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTconsortiaenzymatic hydrolysisempty fruit bunchescellulasesfilamentous fungibiomass.ORIGINAL29874-165678-2-PB.pdfapplication/pdf320256https://repositorio.unal.edu.co/bitstream/unal/40779/1/29874-165678-2-PB.pdf298a2b0b096e6ddffd799677ee0d9a96MD5129874-107982-1-SP.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheet18176https://repositorio.unal.edu.co/bitstream/unal/40779/2/29874-107982-1-SP.xlsx6ca6606da5a8baa994caab415518aa9cMD52THUMBNAIL29874-165678-2-PB.pdf.jpg29874-165678-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg9169https://repositorio.unal.edu.co/bitstream/unal/40779/3/29874-165678-2-PB.pdf.jpga1b59539fccb09d2ae3248f6efde6f8bMD53unal/40779oai:repositorio.unal.edu.co:unal/407792023-01-30 23:04:56.451Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
dc.title.spa.fl_str_mv |
Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest |
title |
Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest |
spellingShingle |
Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest consortia enzymatic hydrolysis empty fruit bunches cellulases filamentous fungi biomass. |
title_short |
Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest |
title_full |
Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest |
title_fullStr |
Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest |
title_full_unstemmed |
Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest |
title_sort |
Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest |
dc.creator.fl_str_mv |
Moya Alvarez, Luz Aida Torres R., Esperanza |
dc.contributor.author.spa.fl_str_mv |
Moya Alvarez, Luz Aida Torres R., Esperanza |
dc.subject.proposal.spa.fl_str_mv |
consortia enzymatic hydrolysis empty fruit bunches cellulases filamentous fungi biomass. |
topic |
consortia enzymatic hydrolysis empty fruit bunches cellulases filamentous fungi biomass. |
description |
Hydrolytic activity was evaluated in a mixture of supernatants produced by filamentous fungi grown individually on microcrystalline cellulose and empty fruit bunches. The strains that were used correspond to two types of isolates; the first was made from soil samples from a transect of a high andean forest of Colombia, in the Parque Natural Nacional de Los Nevados, where, based on previous studies, we selected the strains B7, B11, B11M and B19. The second isolate was obtained from a pool of oil palm empty fruit bunches (Eleaeis guinensis Jacq.) in different states of decomposition on the Unipalma plantation located in the eastern plains; strains TA1 and TA2. To perform the hydrolysis of cellulose and empty fruit bunches, the previously obtained supernatants from each of the selected strains were cultivated for 300 hours in cellulose and characterized individually by endoglucanase, exoglucanase, and β-glucosidase activity. Individual supernatants were mixed at a 1:1 ratio to form consortia; and hydrolytic activity was evaluated in the substrates at two hours. The glucose concentration was determined by the 3,5-dinitrosalicylic acid (DNS) method. The results show that hydrolysis of empty fruit bunch to glucose was favored by three pools of supernatants, with increases greater than 400% in comparison with the hydrolysis obtained by individual supernatants, demonstrating the potential to decompose palm empty fruit bunches; thereby contributing to the reduction of decay time of empty fruit bunches and the decrease of environmental and health problems. |
publishDate |
2012 |
dc.date.issued.spa.fl_str_mv |
2012 |
dc.date.accessioned.spa.fl_str_mv |
2019-06-28T09:40:37Z |
dc.date.available.spa.fl_str_mv |
2019-06-28T09:40:37Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/40779 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/30876/ |
url |
https://repositorio.unal.edu.co/handle/unal/40779 http://bdigital.unal.edu.co/30876/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/agrocol/article/view/29874 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana Agronomía Colombiana |
dc.relation.ispartofseries.none.fl_str_mv |
Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 2357-3732 0120-9965 |
dc.relation.references.spa.fl_str_mv |
Moya Alvarez, Luz Aida and Torres R., Esperanza (2012) Hydrolysis of cellulose and oil palm empty fruit bunches by using consortia of fungi isolated from the soil of colombian high andean forest. Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 Agronomía Colombiana; Vol. 30, núm. 3 (2012); 411-418 2357-3732 0120-9965 . |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial |
institution |
Universidad Nacional de Colombia |
bitstream.url.fl_str_mv |
https://repositorio.unal.edu.co/bitstream/unal/40779/1/29874-165678-2-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/40779/2/29874-107982-1-SP.xlsx https://repositorio.unal.edu.co/bitstream/unal/40779/3/29874-165678-2-PB.pdf.jpg |
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