Microencapsulación de péptidos sintéticos en co-polímeros de poli(láctico-co-glicólico) (PLGA): Efecto de la hidrofobicidad del péptido
imágenes, gráficas, tablas
- Autores:
-
Pabón Ardila, María Laura
- Tipo de recurso:
- Fecha de publicación:
- 2021
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/80299
- Palabra clave:
- 610 - Medicina y salud
540 - Química y ciencias afines
Vacunas
Vaccines
Microencapsulación
PLGA
Hidrofobicidad
Doble emulsión-evaporación del disolvente
Péptidos sintéticos
SPPS
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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UNACIONAL2 |
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| dc.title.spa.fl_str_mv |
Microencapsulación de péptidos sintéticos en co-polímeros de poli(láctico-co-glicólico) (PLGA): Efecto de la hidrofobicidad del péptido |
| dc.title.translated.eng.fl_str_mv |
Microencapsulation of synthetic peptides in poly (lactic-co-glycolic) copolymers (PLGA): Effect of peptide hydrophobicity |
| title |
Microencapsulación de péptidos sintéticos en co-polímeros de poli(láctico-co-glicólico) (PLGA): Efecto de la hidrofobicidad del péptido |
| spellingShingle |
Microencapsulación de péptidos sintéticos en co-polímeros de poli(láctico-co-glicólico) (PLGA): Efecto de la hidrofobicidad del péptido 610 - Medicina y salud 540 - Química y ciencias afines Vacunas Vaccines Microencapsulación PLGA Hidrofobicidad Doble emulsión-evaporación del disolvente Péptidos sintéticos SPPS |
| title_short |
Microencapsulación de péptidos sintéticos en co-polímeros de poli(láctico-co-glicólico) (PLGA): Efecto de la hidrofobicidad del péptido |
| title_full |
Microencapsulación de péptidos sintéticos en co-polímeros de poli(láctico-co-glicólico) (PLGA): Efecto de la hidrofobicidad del péptido |
| title_fullStr |
Microencapsulación de péptidos sintéticos en co-polímeros de poli(láctico-co-glicólico) (PLGA): Efecto de la hidrofobicidad del péptido |
| title_full_unstemmed |
Microencapsulación de péptidos sintéticos en co-polímeros de poli(láctico-co-glicólico) (PLGA): Efecto de la hidrofobicidad del péptido |
| title_sort |
Microencapsulación de péptidos sintéticos en co-polímeros de poli(láctico-co-glicólico) (PLGA): Efecto de la hidrofobicidad del péptido |
| dc.creator.fl_str_mv |
Pabón Ardila, María Laura |
| dc.contributor.advisor.none.fl_str_mv |
Vanegas Murcia, Magnolia Rosas Pérez, Jaiver Eduardo |
| dc.contributor.author.none.fl_str_mv |
Pabón Ardila, María Laura |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo Funcional de Síntesis Química – Fundación Instituto de Inmunología de Colombia –FIDIC |
| dc.subject.ddc.spa.fl_str_mv |
610 - Medicina y salud 540 - Química y ciencias afines |
| topic |
610 - Medicina y salud 540 - Química y ciencias afines Vacunas Vaccines Microencapsulación PLGA Hidrofobicidad Doble emulsión-evaporación del disolvente Péptidos sintéticos SPPS |
| dc.subject.lemb.spa.fl_str_mv |
Vacunas |
| dc.subject.lemb.eng.fl_str_mv |
Vaccines |
| dc.subject.proposal.spa.fl_str_mv |
Microencapsulación PLGA Hidrofobicidad Doble emulsión-evaporación del disolvente Péptidos sintéticos |
| dc.subject.proposal.eng.fl_str_mv |
SPPS |
| description |
imágenes, gráficas, tablas |
| publishDate |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2021-09-24T19:39:54Z |
| dc.date.available.none.fl_str_mv |
2021-09-24T19:39:54Z |
| dc.date.issued.none.fl_str_mv |
2021-09-22 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/80299 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/80299 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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A population-based clinical trial with the spf66 synthetic plasmodium falciparum malaria vaccine in venezuela. J Infect Dis 1994;170:396–402. https://doi.org/10.1093/infdis/170.2.396. Sempértegui F, Estrella B, Moscoso J, Luis Piedrahita C, Hernández D, Gaybor J, et al. Safety, immunogenicity and protective effect of the SPf66 malaria synthetic vaccine against Plasmodium falciparum infection in a randomized double-blind placebo-controlled field trial in an endemic area of Ecuador. Vaccine 1994;12:337–42. https://doi.org/https://doi.org/10.1016/0264-410X(94)90098-1. Beck H ‐P., Felger I, Huber W, Steiger S, Smith T, Weiss N, et al. Analysis of Multiple Plasmodium falciparum Infections in Tanzanian Children during the Phase III Trial of the Malaria Vaccine SPf66. J Infect Dis 1997;175:921–6. https://doi.org/10.1086/513991. D’Alessandro U, Leach A, Olaleye BO, Fegan GW, Jawara M, Langerock P, et al. Efficacy trial of malaria vaccine SPf66 in Gambian infants. 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Poly Lactic-co-Glycolic Acid (PLGA) as biodegradable controlled drug delivery carrier. Polymers (Basel) 2011;3:1377–97. https://doi.org/10.3390/polym3031377. Kapoor DN, Bhatia A, Kaur R, Sharma R, Kaur G, Dhawan S. PLGA: A unique polymer for drug delivery. Ther Deliv 2015. https://doi.org/10.4155/tde.14.91. Anderson JM, Shive MS. Biodegradation and biocompatibility of PLA and PLGA microspheres. Adv Drug Deliv Rev 2012. https://doi.org/10.1016/j.addr.2012.09.004. Lü JM, Wang X, Marin-Muller C, Wang H, Lin PH, Yao Q, et al. Current advances in research and clinical applications of PLGA-based nanotechnology. Expert Rev Mol Diagn 2009;9:325–41. https://doi.org/10.1586/erm.09.15. D’Avila Carvalho Erbetta C. Synthesis and Characterization of Poly(D,L-Lactide-co-Glycolide) Copolymer. J Biomater Nanobiotechnol 2012. https://doi.org/10.4236/jbnb.2012.32027. Kricheldorf HR, Jonté JM, Berl M. Polylactones 3. Copolymerization of glycolide with L, L-lactide and other lactones. 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PS. Synthetic Polypeptides as Antigens, Volume 19. Elsevier Science; 1988. Santoveña A, Oliva A, Guzman F, Patarroyo ME, Llabrés M, Fariña JB. Chromatographic characterization of synthetic peptides: SPf66 malaria vaccine. J Chromatogr B Anal Technol Biomed Life Sci 2002. https://doi.org/10.1016/S0378-4347(01)00392-9. Ito F, Fujimori H, Makino K. Incorporation of water-soluble drugs in PLGA microspheres. Colloids Surfaces B Biointerfaces 2007. https://doi.org/10.1016/j.colsurfb.2006.10.019. ISO 22412. International Standard ISO22412 Particle Size Analysis - Dynamic Light Scattering. 2017. Freiberg S, Zhu XX. Polymer microspheres for controlled drug release. Int J Pharm 2004. https://doi.org/10.1016/j.ijpharm.2004.04.013. Song X, Zhao Y, Hou S, Xu F, Zhao R, He J, et al. Dual agents loaded PLGA nanoparticles: Systematic study of particle size and drug entrapment efficiency. Eur J Pharm Biopharm 2008. https://doi.org/10.1016/j.ejpb.2008.01.013. Feczkó T, Tóth J, Dósa G, Gyenis J. Influence of process conditions on the mean size of PLGA nanoparticles. Chem Eng Process Process Intensif 2011. https://doi.org/10.1016/j.cep.2011.05.006. Gaignaux A, Réeff J, Siepmann F, Siepmann J, De Vriese C, Goole J, et al. Development and evaluation of sustained-release clonidine-loaded PLGA microparticles. Int J Pharm 2012. https://doi.org/10.1016/j.ijpharm.2012.08.006. Liu R, Huang S-S, Wan Y-H, Ma G-H, Su Z-G. Preparation of insulin-loaded PLA/PLGA microcapsules by a novel membrane emulsification method and its release in vitro. Colloids Surf B Biointerfaces 2006;51:30–8. https://doi.org/10.1016/j.colsurfb.2006.05.014. Zhang C, Wu L, Tao A, Bera H, Tang X, Cun D, et al. Formulation and in vitro characterization of long-acting PLGA injectable microspheres encapsulating a peptide analog of LHRH. J Mater Sci Technol 2020. https://doi.org/10.1016/j.jmst.2020.04.020. Yang YY, Chia HH, Chung TS. 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Bhattacharjee S. Understanding the burst release phenomenon: toward designing effective nanoparticulate drug-delivery systems. Ther Deliv 2021. https://doi.org/10.4155/tde-2020-0099. Kohno M, Andhariya J V., Wan B, Bao Q, Rothstein S, Hezel M, et al. The effect of PLGA molecular weight differences on risperidone release from microspheres. Int J Pharm 2020. https://doi.org/10.1016/j.ijpharm.2020.119339. Crotts G, Sah H, Park TG. Adsorption determines in-vitro protein release rate from biodegradable microspheres: Quantitative analysis of surface area during degradation. J Control Release 1997. https://doi.org/10.1016/S0168-3659(96)01624-0. Lu W, Park TG. Protein release from poly(lactic-co-glycolic acid) microspheres: Protein stability problems. PDA J Pharm Sci Technol 1995;49:13–9. Kim HK, Park TG. Microencapsulation of human growth hormone within biodegradable polyester microspheres: Protein aggregation stability and incomplete release mechanism. 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Universidad Nacional de Colombia |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Vanegas Murcia, Magnoliaf484b6abad210b5b93849c6856104244Rosas Pérez, Jaiver Eduardod998f772bc3d8f602a106bc9e589eac8Pabón Ardila, María Laurae8d6e7b4ca4f2a7bffb6ab278d83ef09Grupo Funcional de Síntesis Química – Fundación Instituto de Inmunología de Colombia –FIDIC2021-09-24T19:39:54Z2021-09-24T19:39:54Z2021-09-22https://repositorio.unal.edu.co/handle/unal/80299Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/imágenes, gráficas, tablasEn el presente estudio se evaluó el efecto de la hidrofobicidad de péptidos sintéticos durante su microencapsulación en sistemas microparticulares elaborados con PLGA. De la base de datos de la Fundación Instituto de Inmunología de Colombia (FIDIC), fueron preseleccionadas 280 secuencias peptídicas diseñadas y evaluadas como potenciales candidatos en la búsqueda de una vacuna sintética contra la malaria, y se clasificaron en tres grupos, según su índice de hidropaticidad (hidrofílico, neutro e hidrofóbico). De estas, seis secuencias (dos por cada grupo) fueron seleccionadas y sintetizadas por síntesis de péptidos en fase sólida (SPPS) mediante la estrategia t-Boc/Bzl y fueron adecuadamente caracterizadas y purificadas. Posteriormente, estos péptidos se encapsularon en micropartículas elaboradas con el co-polímero PLGA 50:50 por la técnica de doble emulsión-evaporación del disolvente (W/O/W). Los sistemas microparticulares fueron evaluados en su tamaño, forma, carga, eficiencia de encapsulación y el perfil de liberación in vitro. Los resultados de este trabajo indicaron que independientemente del índice de hidropatía de las secuencias, fue posible obtener estos péptidos con alto grado de pureza mediante la metodología SPPS estrategia t-Boc/Bzl. Además, que el carácter hidrofóbico del péptido encapsulado afectó en mayor o menor proporción en cada una de las variables evaluadas en los sistemas microparticulares. (Texto tomado de la fuente)In the present study the effect of the hydrophobicity of synthetic peptides during microencapsulation in microparticulate systems based on PLGA was evaluated. From the database of the Fundación Instituto de Inmunología de Colombia (FIDIC) were preselected 280 peptide sequences designed and evaluated as potential candidates in the search for a synthetic vaccine against malaria and were classified into three groups, according to their hydropathic index (hydrophilic, neutral, and hydrophobic). Of these, six sequences (two for each group) were selected and synthesized by solid phase peptide synthesis (SPPS) using the t-Boc/Bzl strategy and were adequately characterized and purified. These peptides were subsequently encapsulated in microparticles prepared with the PLGA 50:50 copolymer by the double emulsion-solvent evaporation technique (W/O/W). The microparticulate systems were evaluated for size, shape, charge, encapsulation efficiency, and in vitro release profile. The results of this work indicated that regardless of the hydropathic index of the sequence it was possible to obtain these peptides with a high degree of purity by the SPPS methodology t-Boc/Bzl strategy. Furthermore, the hydrophobic character of the encapsulated peptides affected to a greater or lesser extent each of the variables evaluated in the microparticulate systems, being more relevant in the charge, encapsulation efficiency and in the release profile.MaestríaMagister en Ciencias - MicrobiologíaBioprocesos y bioprospecciónxix, 100 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaInstituto de Biotecnología (IBUN)Facultad de CienciasBogotá - ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud540 - Química y ciencias afinesVacunasVaccinesMicroencapsulaciónPLGAHidrofobicidadDoble emulsión-evaporación del disolventePéptidos sintéticosSPPSMicroencapsulación de péptidos sintéticos en co-polímeros de poli(láctico-co-glicólico) (PLGA): Efecto de la hidrofobicidad del péptidoMicroencapsulation of synthetic peptides in poly (lactic-co-glycolic) copolymers (PLGA): Effect of peptide hydrophobicityTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMWagner AM, Gran MP, Peppas NA. 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