Detección electroquímica de α-Pirrolidinopentiofenona (α-PVP) Y 3,4-Metilendioximetanfetamina (MDMA) mediante un biosensor electroquímico basado en la isoenzima 2D6 del citocromo P450
ilustraciones, gráficas
- Autores:
-
Lugo Vargas, Andrés Felipe
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/81049
- Palabra clave:
- 540 - Química y ciencias afines::543 - Química analítica
Amphetamines
Toxicology/methods
Anfetaminas
Toxicología/métodos
Cytochromes
Citocromos
Técnicas biosensibles
Electroquímica
Análisis químico
Drogas de calle
Drogas de diseño
Narcóticos
Electrochemistry
Street drugs
Biosensing techniques
Chemical analysis
Designer drugs
Narcotics
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Detección electroquímica de α-Pirrolidinopentiofenona (α-PVP) Y 3,4-Metilendioximetanfetamina (MDMA) mediante un biosensor electroquímico basado en la isoenzima 2D6 del citocromo P450 |
| dc.title.translated.eng.fl_str_mv |
Electrochemical detection of α-Pyrrolidinopentiophenone (α-PVP) and 3,4-Methylenedioxymethamphetamine (MDMA) using an electrochemical biosensor based on cytochrome P450 isoenzyme 2D6 |
| title |
Detección electroquímica de α-Pirrolidinopentiofenona (α-PVP) Y 3,4-Metilendioximetanfetamina (MDMA) mediante un biosensor electroquímico basado en la isoenzima 2D6 del citocromo P450 |
| spellingShingle |
Detección electroquímica de α-Pirrolidinopentiofenona (α-PVP) Y 3,4-Metilendioximetanfetamina (MDMA) mediante un biosensor electroquímico basado en la isoenzima 2D6 del citocromo P450 540 - Química y ciencias afines::543 - Química analítica Amphetamines Toxicology/methods Anfetaminas Toxicología/métodos Cytochromes Citocromos Técnicas biosensibles Electroquímica Análisis químico Drogas de calle Drogas de diseño Narcóticos Electrochemistry Street drugs Biosensing techniques Chemical analysis Designer drugs Narcotics |
| title_short |
Detección electroquímica de α-Pirrolidinopentiofenona (α-PVP) Y 3,4-Metilendioximetanfetamina (MDMA) mediante un biosensor electroquímico basado en la isoenzima 2D6 del citocromo P450 |
| title_full |
Detección electroquímica de α-Pirrolidinopentiofenona (α-PVP) Y 3,4-Metilendioximetanfetamina (MDMA) mediante un biosensor electroquímico basado en la isoenzima 2D6 del citocromo P450 |
| title_fullStr |
Detección electroquímica de α-Pirrolidinopentiofenona (α-PVP) Y 3,4-Metilendioximetanfetamina (MDMA) mediante un biosensor electroquímico basado en la isoenzima 2D6 del citocromo P450 |
| title_full_unstemmed |
Detección electroquímica de α-Pirrolidinopentiofenona (α-PVP) Y 3,4-Metilendioximetanfetamina (MDMA) mediante un biosensor electroquímico basado en la isoenzima 2D6 del citocromo P450 |
| title_sort |
Detección electroquímica de α-Pirrolidinopentiofenona (α-PVP) Y 3,4-Metilendioximetanfetamina (MDMA) mediante un biosensor electroquímico basado en la isoenzima 2D6 del citocromo P450 |
| dc.creator.fl_str_mv |
Lugo Vargas, Andrés Felipe |
| dc.contributor.advisor.spa.fl_str_mv |
Martínez Suárez, Jaime Fernando Chaves Silva, Diana Carolina Quevedo Buitrago, William Giovanni |
| dc.contributor.author.spa.fl_str_mv |
Lugo Vargas, Andrés Felipe |
| dc.contributor.researchgroup.spa.fl_str_mv |
Sustancias Psicoactivas |
| dc.subject.ddc.spa.fl_str_mv |
540 - Química y ciencias afines::543 - Química analítica |
| topic |
540 - Química y ciencias afines::543 - Química analítica Amphetamines Toxicology/methods Anfetaminas Toxicología/métodos Cytochromes Citocromos Técnicas biosensibles Electroquímica Análisis químico Drogas de calle Drogas de diseño Narcóticos Electrochemistry Street drugs Biosensing techniques Chemical analysis Designer drugs Narcotics |
| dc.subject.decs.eng.fl_str_mv |
Amphetamines Toxicology/methods |
| dc.subject.decs.spa.fl_str_mv |
Anfetaminas Toxicología/métodos |
| dc.subject.lemb.eng.fl_str_mv |
Cytochromes |
| dc.subject.lemb.spa.fl_str_mv |
Citocromos |
| dc.subject.proposal.spa.fl_str_mv |
Técnicas biosensibles Electroquímica Análisis químico Drogas de calle Drogas de diseño Narcóticos |
| dc.subject.proposal.eng.fl_str_mv |
Electrochemistry Street drugs Biosensing techniques Chemical analysis Designer drugs Narcotics |
| description |
ilustraciones, gráficas |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-02-23T20:53:03Z |
| dc.date.available.none.fl_str_mv |
2022-02-23T20:53:03Z |
| dc.date.issued.none.fl_str_mv |
2022-02 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/81049 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/81049 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Martínez Suárez, Jaime Fernandoe773ec04a44b290218d429e737aca9ea600Chaves Silva, Diana Carolinadaddc2377748a3728a8888d9e3783f30600Quevedo Buitrago, William Giovanniacdfe5e0cf37c2a55607112a36412f33Lugo Vargas, Andrés Felipe2628132ea2d8bdf28d556301f1d58847600Sustancias Psicoactivas2022-02-23T20:53:03Z2022-02-23T20:53:03Z2022-02https://repositorio.unal.edu.co/handle/unal/81049Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, gráficasEn el presente estudio se exploró mediante la técnica de voltamperometría diferencial de pulsos (VDP) la respuesta electroquímica de α-pirrolidinopentiofenona (α-PVP) y 3,4-metilendioximetanfetamina (MDMA) frente a un biosensor electroquímico basado a en la isoforma CYP2D6 del citocromo P450 unida a través de enlace covalente a la superficie de carbono de electrodos serigrafiados (Electrodo de trabajo: C, electrodo auxiliar: C, electrodo de referencia: Ag/AgCl). El biosensor fue caracterizado mediante cupla ferro/ferricianuro de potasio y pruebas de inhibición con quinidina. Como resultado se evidenció una respuesta de los analitos frente al biosensor electroquímico relacionada con la actividad enzima-sustrato, con una respuesta directamente proporcional y con tendencia lineal entre la concentración del analito y la corriente de pico catódico; los límites de deteccion y cuantificación obtenidos para el analito MDMA fueron 0,0085 µM (1,64 ng/mL) y 0,028 µM (5,41 ng/mL) respectivamente, para el analito α-PVP los límites de detección y cuantificación fueron 0,0099 µM (2,2 ng/mL) y 0,033 µM (7,6 ng/mL), respectivamente. Finalmente se estudió la interferencia generada por otra especie diferente al analito de interés en matrices binarias (α-PVP +MDMA, α-PVP + cafeína y MDMA + cafeína) donde se observó que la detección de ambos analitos de interés en una misma matriz no fue posible, sin embargo, la presencia de cafeína no representó una interferencia para el análisis de MDMA. (Texto tomado de la fuente).In this study, the electrochemical response of α-Pyrrolidinopentiophenone (α-PVP) and 3,4-Methylenedioxymethamphetamine (MDMA) was explored using differential pulse voltammetry against an electrochemical biosensor based on the CYP2D6 isoform of cytochrome P450 bound via covalent bond to the carbon surface of screen-printed electrodes (Working electrode: C, auxiliary electrode: C, reference electrode: Ag/AgCl). The biosensor was characterized by potassium ferro/ferricyanide couple and quinidine inhibition tests. A response of the analytes to the electrochemical biosensor related to the enzyme-substrate activity could be evidenced, with a directly proportional response and with a linear trend between the concentration of the analyte and the cathodic peak current; the detection and quantification limits obtained for the MDMA analyte were 0.0085 μM (1,64 ng/mL) and 0.028 μM (5,41 ng/mL) respectively, for the α-PVP analyte the detection and quantification limits were 0.0099 μM (2,2 ng/mL) and 0.033 μM (7,6 ng/mL), respectively. Finally, the interference generated by another species different from the analyte of interest in binary matrices (α-PVP + MDMA, α-PVP + caffeine and MDMA + caffeine) was studied, where it was observed that the detection of both analytes of interest in the same matrix did not was possible, however, the presence of caffeine did not represent an interference for the analysis of MDMA.MaestríaMagíster en ToxicologíaAnálisis toxicológico de laboratorio en sustancias psicoactivasxxi, 115 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina - Maestría en ToxicologíaDepartamento de ToxicologíaFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá540 - Química y ciencias afines::543 - Química analíticaAmphetaminesToxicology/methodsAnfetaminasToxicología/métodosCytochromesCitocromosTécnicas biosensiblesElectroquímicaAnálisis químicoDrogas de calleDrogas de diseñoNarcóticosElectrochemistryStreet drugsBiosensing techniquesChemical analysisDesigner drugsNarcoticsDetección electroquímica de α-Pirrolidinopentiofenona (α-PVP) Y 3,4-Metilendioximetanfetamina (MDMA) mediante un biosensor electroquímico basado en la isoenzima 2D6 del citocromo P450Electrochemical detection of α-Pyrrolidinopentiophenone (α-PVP) and 3,4-Methylenedioxymethamphetamine (MDMA) using an electrochemical biosensor based on cytochrome P450 isoenzyme 2D6Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMUNODC. 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Talanta. 2021;234(June):122658.DETERMINACIÓN DE CATINONAS SINTETICAS EN MATERIAL INCAUTADO EN COLOMBIA MEDIANTE UN BIOSENSOR ELECTROQUÍMICO BASADO EN LA ENZIMA CYP2D6 (código Hermes 48279)Vicerrectoría de investigaciones de la sede Bogotá de la Universidad Nacional de Colombia en el marco de la convocatoria de apoyo a proyectos de investigación y creación artística de la sede Bogotá de la Universidad Nacional de Colombia (2019)InvestigadoresPúblico generalORIGINAL1117531447.2022.pdf1117531447.2022.pdfTesis de Maestría en Toxicologíaapplication/pdf4001412https://repositorio.unal.edu.co/bitstream/unal/81049/3/1117531447.2022.pdf2da1a6f2418b0168b645929141374222MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81049/4/license.txt8153f7789df02f0a4c9e079953658ab2MD54THUMBNAIL1117531447.2022.pdf.jpg1117531447.2022.pdf.jpgGenerated 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