Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación

diagramas, ilustraciones a color, tablas

Autores:: Rodríguez Beltrán, Edgar Orlando

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación
dc.title.translated.eng.fl_str_mv	rpoB S531L-rifampicin-resistant mutant isolation in Mycobacterium tuberculosis ATCC 25618 and evolution biomarker identification
title	Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación
spellingShingle	Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación 610 - Medicina y salud::615 - Farmacología y terapéutica Rifampin Antibióticos Antituberculosos Antibiotics, Antitubercular Mycobacterium Mycobacterium tuberculosis Rifampicina rpoB Fitness Virulencia Resistencia Antibióticos Rifampicin Fitness Virulence Resistance Antibiotics
title_short	Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación
title_full	Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación
title_fullStr	Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación
title_full_unstemmed	Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación
title_sort	Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación
dc.creator.fl_str_mv	Rodríguez Beltrán, Edgar Orlando
dc.contributor.advisor.none.fl_str_mv	Murcia Aranguren, Martha Isabel Carazzone, Chiara
dc.contributor.author.none.fl_str_mv	Rodríguez Beltrán, Edgar Orlando
dc.contributor.projectmanager.none.fl_str_mv	Edgar Orlando Rodríguez Beltrán
dc.contributor.researchgroup.spa.fl_str_mv	MICOBAC-UN
dc.subject.ddc.spa.fl_str_mv	610 - Medicina y salud::615 - Farmacología y terapéutica
topic	610 - Medicina y salud::615 - Farmacología y terapéutica Rifampin Antibióticos Antituberculosos Antibiotics, Antitubercular Mycobacterium Mycobacterium tuberculosis Rifampicina rpoB Fitness Virulencia Resistencia Antibióticos Rifampicin Fitness Virulence Resistance Antibiotics
dc.subject.decs.none.fl_str_mv	Rifampin Antibióticos Antituberculosos Antibiotics, Antitubercular Mycobacterium
dc.subject.proposal.spa.fl_str_mv	Mycobacterium tuberculosis Rifampicina rpoB Fitness Virulencia Resistencia Antibióticos
dc.subject.proposal.eng.fl_str_mv	Rifampicin Fitness Virulence Resistance Antibiotics
description	diagramas, ilustraciones a color, tablas
publishDate	2021
dc.date.accessioned.none.fl_str_mv	2021-06-08T20:55:33Z
dc.date.available.none.fl_str_mv	2021-06-08T20:55:33Z
dc.date.issued.none.fl_str_mv	2021
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/79617
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/79617 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.format.extent.spa.fl_str_mv	1 recurso en línea (235 páginas)
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias - Maestría en Ciencias - Microbiología
dc.publisher.department.spa.fl_str_mv	Instituto de Biotecnología (IBUN)
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Murcia Aranguren, Martha Isabel2d3a40be45d44dfb2c92cd107332178cCarazzone, Chiaraa16885e2b62003869c663402913aaf32600Rodríguez Beltrán, Edgar Orlando2e033cfd3c7f5582588d68ccb468a9b2Edgar Orlando Rodríguez BeltránMICOBAC-UN2021-06-08T20:55:33Z2021-06-08T20:55:33Z2021https://repositorio.unal.edu.co/handle/unal/79617Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/diagramas, ilustraciones a color, tablasLa TB multirresistente es causada por Mycobacterium tuberculosis (MTB) resistente a rifampicina e isoniazida y constituye un grave problema de salud pública en países emergentes. Puesto que el fitness del agente patógeno es crítico en el desenlace en la infección, el conocimiento sobre el mismo debe ser claro. Trabajos previos sobre resistencia a rifampicina han mostrado situaciones de pérdida y otras de conservación del fitness competitivo. El objetivo del trabajo fue medir la expresión de genes de virulencia / resistencia y los niveles de PDIM relacionándolos con el fitness competitivo de una cepa ATCC25618 RR-TB. Materiales y métodos: Se realizó un ensayo de fitness competitivo usando las cepas MTB ATCC25618 wt: ATCC25618 rpoB-S450L (o S531L) (RifR 1 µg/ml) 1:1 en 50 ml Middlebrook 7H9/OADC (Días 2-12) calculándose tiempos de generación in vitro y fitness relativo. Se extrajeron mRNA y PDIM en días 10/11 y 21 Resultados: Se obtuvo la cepa ATCC 25618 rpoB-S450L (transición en rpoB C1349T). Disminuyó el fitness en rpoB-S450L yH445Y mostrándose fitness dispar interréplicas biológicas. En S450L, hubo dos réplicas con disminución del fitness y una con aumento. Hubo aumento significativo de la expresión de pknG en rpoB-S450L vs silvestre y un aumento no significativo en la cepa silvestre (comparada con rpoB-S450L) en la expresión de ppsA en fase logarítmica que se correlaciona con mayor producción de PDIM, siendo pknG y PDIM potenciales biomarcadoresIntroduction. Multidrug-resistant TB, the infection by INH/ RIF-resistant tuberculosis is a severe public health problem in emerging countries. Fitness (W) is a critical component in clinical outcome so it must be explored. Previous works on RIF-resistance have shown some competitive fitness gains and losses. Objective. The objective of these experiments was to measure virulence/ resistance genes and PDIM levels in relation with competitive fitness of a S450L RifR ATCC25618 strain. Materials and methods. We made a competitive fitness in vitro assay with ATCC 25618 wt: ATCC 25618 rpoB-S450L (RifR 1 µg/ml) 1:1 strains in 50 ml Middlebrook OADC and we found generation time (G) in vitro and relative fitness. mRNA and PDIM were extracted on days 10/11 and 21. Results. We obtained ATCC 25618 rpoB S450L mutant (rpoB C1349T transition). Fitness decreased in rpoB-S450L strain with heterogeneous fitness in 3 biological replicas for both S450L and H445Y strains, for the former, 2 replicas with low W and one with high W. There was significant pknG increase in rpoB-S450L and non-significant increase in wt (compared to S450L) in polyketide synthase-ppsA in log phase which is related to increased PDIM, being PDIM and pknG potential biomarkers.Proyecto 120771250393 con contrato 295/2016 de Colciencias (actual MinCien-cias)MaestríaMagíster en Ciencias - MicrobiologíaSe realizó un ensayo de fitness competitivo usando las cepas MTB ATCC25618 wt: ATCC25618 rpoB-S450L (o S531L) (RifR 1 ug/ml) 1:1 en 50 ml Middlebrook 7H9/OADC (Días 2-12) calculándose tiempos de generación in vitro y fitness relativo. Se extrajeron mRNA y PDIM en días 10/11 y 21Biología Molecular de los Microorganismos1 recurso en línea (235 páginas)application/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaInstituto de Biotecnología (IBUN)Facultad de CienciasBogotáUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::615 - Farmacología y terapéuticaRifampinAntibióticos AntituberculososAntibiotics, AntitubercularMycobacteriumMycobacterium tuberculosisRifampicinarpoBFitnessVirulenciaResistenciaAntibióticosRifampicinFitnessVirulenceResistanceAntibioticsAislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptaciónrpoB S531L-rifampicin-resistant mutant isolation in Mycobacterium tuberculosis ATCC 25618 and evolution biomarker identificationTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAdone, R., Ciuchini, F., & Marianelli, C. 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Molecular Cell, 50(3), 430–436. https://doi.org/10.1016/j.molcel.2013.03.020Identificación de biomarcadores de evolución y adaptación de tuberculosis resistente a rifampicina basada en expresión de genes de resistencia/ virulencia/ fitness y en lipidomica comparativa: qRT-PCR y HPLC-MMinCiencias-República de ColombiaLICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/79617/1/license.txtcccfe52f796b7c63423298c2d3365fc6MD51ORIGINALunMS09tesis70-Documento de tesis definitivo.pdfunMS09tesis70-Documento de tesis definitivo.pdfTesis de Maestría en Ciencias - Microbiologíaapplication/pdf8103238https://repositorio.unal.edu.co/bitstream/unal/79617/2/unMS09tesis70-Documento%20de%20tesis%20definitivo.pdf502d3f22a10003caa6732e7e6991780fMD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8799https://repositorio.unal.edu.co/bitstream/unal/79617/3/license_rdff7d494f61e544413a13e6ba1da2089cdMD53THUMBNAILunMS09tesis70-Documento de 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GVyZWNob3MgZGUgYXV0b3IgcXVlIGNvbmxsZXZlIGxhIGRpc3RyaWJ1Y2nDs24gZGUgZXN0b3MgYXJjaGl2b3MgeSBtZXRhZGF0b3MuCkFsIGhhY2VyIGNsaWMgZW4gZWwgc2lndWllbnRlIGJvdMOzbiwgdXN0ZWQgaW5kaWNhIHF1ZSBlc3TDoSBkZSBhY3VlcmRvIGNvbiBlc3RvcyB0w6lybWlub3MuCgpVTklWRVJTSURBRCBOQUNJT05BTCBERSBDT0xPTUJJQSAtIMOabHRpbWEgbW9kaWZpY2FjacOzbiAyNy8yMC8yMDIwCg==

Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación

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