Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum

The effect of extracts, essential oil, and their major constituents from parsley (Petroselinum crispum (Mill.) Fuss.) against the phytopathogenic fungus Collectotrichum acutatum was evaluated by the poisoned agar method. Results showed that all extracts, along with the essential oil, significantly i...

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Autores:
Pineda, Rodrigo
Vizcaíno, Samuel
García, Carlos M.
Gil, Jesús H.
Durango, Diego L.
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/65944
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/65944
http://bdigital.unal.edu.co/66967/
Palabra clave:
63 Agricultura y tecnologías relacionadas / Agriculture
Apiaceae
Perejil
Extractos
Aceite esencial
CG-EM
Parsley-apiol
Apiaceae
Parsley
Extracts
Essential oil
GC-MS
Parsley-apiole
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_f33e960a8df077d95fc93648c7620051
oai_identifier_str oai:repositorio.unal.edu.co:unal/65944
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum
title Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum
spellingShingle Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum
63 Agricultura y tecnologías relacionadas / Agriculture
Apiaceae
Perejil
Extractos
Aceite esencial
CG-EM
Parsley-apiol
Apiaceae
Parsley
Extracts
Essential oil
GC-MS
Parsley-apiole
title_short Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum
title_full Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum
title_fullStr Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum
title_full_unstemmed Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum
title_sort Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum
dc.creator.fl_str_mv Pineda, Rodrigo
Vizcaíno, Samuel
García, Carlos M.
Gil, Jesús H.
Durango, Diego L.
dc.contributor.author.spa.fl_str_mv Pineda, Rodrigo
Vizcaíno, Samuel
García, Carlos M.
Gil, Jesús H.
Durango, Diego L.
dc.subject.ddc.spa.fl_str_mv 63 Agricultura y tecnologías relacionadas / Agriculture
topic 63 Agricultura y tecnologías relacionadas / Agriculture
Apiaceae
Perejil
Extractos
Aceite esencial
CG-EM
Parsley-apiol
Apiaceae
Parsley
Extracts
Essential oil
GC-MS
Parsley-apiole
dc.subject.proposal.spa.fl_str_mv Apiaceae
Perejil
Extractos
Aceite esencial
CG-EM
Parsley-apiol
Apiaceae
Parsley
Extracts
Essential oil
GC-MS
Parsley-apiole
description The effect of extracts, essential oil, and their major constituents from parsley (Petroselinum crispum (Mill.) Fuss.) against the phytopathogenic fungus Collectotrichum acutatum was evaluated by the poisoned agar method. Results showed that all extracts, along with the essential oil, significantly inhibit the radial growth of C. acutatum at concentrations higher than 100 μg mL-1. The higher activity was found for the essential oil followed by the n-hexane extract. Analysis by gas chromatography with mass spectroscopy (GC-MS) of n-hexane extract and the essential oil of P. crispum showed that the major components correspond to the phenylpropanoids myristicin and parsley-apiole. Both compounds were isolated by conventional chromatographic techniques and their structures elucidated by spectroscopicmethods. Myristicin and parsley-apiole displayed a significant inhibitory effect against C. acutatum. The highest fungistatic activity was found to parsley-apiole with IC50 value of 40 μg mL-1. In conclusion, parsley may be a good source of antifungal compounds to control C. acutatum.
publishDate 2018
dc.date.issued.spa.fl_str_mv 2018-09-01
dc.date.accessioned.spa.fl_str_mv 2019-07-03T01:12:14Z
dc.date.available.spa.fl_str_mv 2019-07-03T01:12:14Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.issn.spa.fl_str_mv ISSN: 2248-7026
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/65944
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identifier_str_mv ISSN: 2248-7026
url https://repositorio.unal.edu.co/handle/unal/65944
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dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/refame/article/view/68284
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía Medellín
Revista Facultad Nacional de Agronomía Medellín
dc.relation.references.spa.fl_str_mv Pineda, Rodrigo and Vizcaíno, Samuel and García, Carlos M. and Gil, Jesús H. and Durango, Diego L. (2018) Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum. Revista Facultad Nacional de Agronomía Medellín, 71 (3). pp. 8563-8572. ISSN 2248-7026
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Medellín - Facultad de Ciencias Agrarias
institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Pineda, Rodrigo53ada110-6750-404c-ac46-531d979423c7300Vizcaíno, Samuel974b81cf-616a-4886-858f-8a5de7836ed7300García, Carlos M.933979f5-c912-4ff7-963b-82d3f133f2c2300Gil, Jesús H.0b278ec3-ae9d-4ceb-b9c6-8b4c371dd842300Durango, Diego L.3e445914-1a74-4bc8-8d04-1ff239eece223002019-07-03T01:12:14Z2019-07-03T01:12:14Z2018-09-01ISSN: 2248-7026https://repositorio.unal.edu.co/handle/unal/65944http://bdigital.unal.edu.co/66967/The effect of extracts, essential oil, and their major constituents from parsley (Petroselinum crispum (Mill.) Fuss.) against the phytopathogenic fungus Collectotrichum acutatum was evaluated by the poisoned agar method. Results showed that all extracts, along with the essential oil, significantly inhibit the radial growth of C. acutatum at concentrations higher than 100 μg mL-1. The higher activity was found for the essential oil followed by the n-hexane extract. Analysis by gas chromatography with mass spectroscopy (GC-MS) of n-hexane extract and the essential oil of P. crispum showed that the major components correspond to the phenylpropanoids myristicin and parsley-apiole. Both compounds were isolated by conventional chromatographic techniques and their structures elucidated by spectroscopicmethods. Myristicin and parsley-apiole displayed a significant inhibitory effect against C. acutatum. The highest fungistatic activity was found to parsley-apiole with IC50 value of 40 μg mL-1. In conclusion, parsley may be a good source of antifungal compounds to control C. acutatum.Se evaluó el efecto de extractos, el aceite esencial y los componentes principales del perejil (Petroselinum crispum (Mill.) Fuss.) contra el hongo fitopatógeno Collectotrichum acutatum mediante el método del agar envenenado. Los resultados mostraron que todos los extractos, junto con elaceite esencial, inhibieron significativamente el crecimiento radial de C. acutatum en concentraciones superiores a 100 μg mL-1. La mayor actividad se encontró para el aceite esencial, seguido por el extracto de n-hexano. El análisis por cromatografía de gases con espectroscopia de masas (CG-EM)del extracto de n-hexano y el aceite esencial de P. crispum mostró que los componentes principales corresponden a los fenilpropanoides miristicina y parsley-apiol. Ambos compuestos fueron aislaron por técnicas cromatográficas convencionales y sus estructuras elucidadas por métodos espectroscópicos. La miristicina y el parsley-apiol mostraron un efecto inhibidor significativo contra C. acutatum. La actividad fungistática más alta se encontró para parsley-apiol con un valor CI50 de 40 μg mL-1. Enconclusión, el perejil puede ser una buena fuente de compuestos antimicóticos para controlar el C. acutatum.application/pdfspaUniversidad Nacional de Colombia - Sede Medellín - Facultad de Ciencias Agrariashttps://revistas.unal.edu.co/index.php/refame/article/view/68284Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía MedellínRevista Facultad Nacional de Agronomía MedellínPineda, Rodrigo and Vizcaíno, Samuel and García, Carlos M. and Gil, Jesús H. and Durango, Diego L. (2018) Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum. Revista Facultad Nacional de Agronomía Medellín, 71 (3). pp. 8563-8572. ISSN 2248-702663 Agricultura y tecnologías relacionadas / AgricultureApiaceaePerejilExtractosAceite esencialCG-EMParsley-apiolApiaceaeParsleyExtractsEssential oilGC-MSParsley-apioleAntifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatumArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL68284-396099-1-PB.pdfapplication/pdf347010https://repositorio.unal.edu.co/bitstream/unal/65944/1/68284-396099-1-PB.pdfb4bf494013e54661dddbdf10c544b009MD51THUMBNAIL68284-396099-1-PB.pdf.jpg68284-396099-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg7743https://repositorio.unal.edu.co/bitstream/unal/65944/2/68284-396099-1-PB.pdf.jpg79272828f9da93fa885d1bd98fb94bbfMD52unal/65944oai:repositorio.unal.edu.co:unal/659442023-05-21 23:14:18.769Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co