Natural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group Phureja

ilustraciones, graficas, tablas

Autores:: Diaz Valencia, Paula Andrea

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2022

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: eng

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.eng.fl_str_mv	Natural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group Phureja
dc.title.translated.spa.fl_str_mv	Diversidad alelica natual y senales respuestas asociadas con la tolerancia al deficit hidrico en Solanum tuberosum Grupo Phureja
title	Natural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group Phureja
spellingShingle	Natural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group Phureja 630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura) ALMIDON DE PAPA-ANALISIS Potato starch - analysis Tolerance water deficit association mapping early signal Tolerancia deficit hidrico mapeo por asociacion senales tempranas
title_short	Natural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group Phureja
title_full	Natural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group Phureja
title_fullStr	Natural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group Phureja
title_full_unstemmed	Natural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group Phureja
title_sort	Natural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group Phureja
dc.creator.fl_str_mv	Diaz Valencia, Paula Andrea
dc.contributor.advisor.none.fl_str_mv	Mosquera Vasquez, Teresa
dc.contributor.author.none.fl_str_mv	Diaz Valencia, Paula Andrea
dc.contributor.researcher.none.fl_str_mv	Melgarejo Luz Marina
dc.contributor.researchgroup.spa.fl_str_mv	Genética de Rasgos de Interés Agronómico
dc.subject.ddc.spa.fl_str_mv	630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura)
topic	630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura) ALMIDON DE PAPA-ANALISIS Potato starch - analysis Tolerance water deficit association mapping early signal Tolerancia deficit hidrico mapeo por asociacion senales tempranas
dc.subject.lemb.spa.fl_str_mv	ALMIDON DE PAPA-ANALISIS
dc.subject.lemb.eng.fl_str_mv	Potato starch - analysis
dc.subject.proposal.eng.fl_str_mv	Tolerance water deficit association mapping early signal
dc.subject.proposal.spa.fl_str_mv	Tolerancia deficit hidrico mapeo por asociacion senales tempranas
description	ilustraciones, graficas, tablas
publishDate	2022
dc.date.accessioned.none.fl_str_mv	2022-06-14T16:09:25Z
dc.date.available.none.fl_str_mv	2022-06-14T16:09:25Z
dc.date.issued.none.fl_str_mv	2022-06-08
dc.type.spa.fl_str_mv	Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TD
format	http://purl.org/coar/resource_type/c_db06
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/81580
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/81580 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.indexed.spa.fl_str_mv	Agrovoc
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
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institution	Universidad Nacional de Colombia
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Mosquera Vasquez, Teresa4f7b8f77511417b707fec67d524baccdDiaz Valencia, Paula Andrea1188b72ff3725a7e8977e2300f93f956Melgarejo Luz MarinaGenética de Rasgos de Interés Agronómico2022-06-14T16:09:25Z2022-06-14T16:09:25Z2022-06-08https://repositorio.unal.edu.co/handle/unal/81580Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, graficas, tablasGenetic improvement of diploid potato (Solanum tuberosum Group Phureja) for water deficit tolerance and tuber yield is challenging because of the complex nature of these traits. In order to develop a research that cconsiders the complexity of the trait and simultaneously to make the experiments more tractable, we conducted two studies. The first was conducted to investigate the genetic architecture of the water deficit tolerance by employing the Working Collection of the Potato Breeding Program at the Universidad Nacional de Colombia. A diversity panel of 104 diploid potato accessions were evaluated under both well-watered and water deficit treatments at tuber initiation stage. The response to water deficit conditions was assessed with the relative chlorophyll content (CC), the maximum quantum efficiency of PSII (Fv/Fm), relative water content (RWC), leaf sugar content, tuber number per plant (TN), and tuber fresh weight per plant (TW). The phenotypic evaluation results revealed that the physiological, biochemical and yield-component variables had a broad variation, while the yield-component variables more powerfully distinguished between the tolerant and susceptible genotypes than the physiological and biochemical variables. The multivariate analysis based on the Drought Tolerance Index (DTI) revealed different levels of water deficit tolerance for the 104 genotypes. The Genome-Wide Association Studies (GWAS) was conducted using a matrix of 47 K single nucleotide polymorphisms (SNP), recently available for this population. We are reporting 38 Quantitative Trait Loci (QTL), seven for well-watered conditions, twenty-two for water deficit condition,s and nine for DTI which explain between 12.6% and 44.1% of the phenotypic variance. A set of 6 QTL were found to be associated with more than one variable. Of the nine QTL detected from DTI on chromosomes 2, 3, 5, 8, 10 and 12, three candidate genes with a feasible role in water deficit response were identified. These results provide a foundation for future research directed at understanding the molecular mechanisms underlying potato tolerance to water deficits, and the QTL identified could be used in marker-assisted selection (MAS) for water-deficit tolerance breeding in potato. In the second study, we investigated the physiological, biochemical and yield-related variables of four contrasting water-deficit tolerance diploid potato genotypes to water deficit with emphasis on temporal trend responses. Comparative analysis successfully identified that the physiological changes were faster and pronounced in the water-deficit sensitive genotypes. In contrast, in the tolerant genotypes water deficit induced earlier and more remarkable accumulation of sucrose and glucose. The observed temporal response showed that statistical differences between tolerant and sensitive genotypes in ψl, CC, and soluble sugar content can be distinguished from 3DAT. In general, the temporal variation in physiological and biochemical parameters demonstrated the presence of different strategies among tolerant genotypes. These results can contribute to a better understanding of the temporal physiological and biochemical mechanisms leading to water-deficit tolerance and will help potato breeding programs with improved stress tolerance and stable yields under changing climate conditions without the loss of yield potential under optimal conditions. The results of this study provide insights into the nature of genetic variations governing water deficit tolerance in diploid potato. Knowledge gained of the dissection may be utilized in breeding programs to select lines with improved yield and water deficit tolerance. Additionally, the information from the panel SNP polymorphisms and candidate gene detection provides research avenues to further refine/narrow down genomic regions associated with these agronomically important traits, and also opens gates for genetic enhancement through genomic aided selection.El mejoramiento genético de la papa diploide (Solanum tuberosum Group Phureja) para tolerancia al déficit de agua y rendimiento de tubérculos es un desafío debido a la naturaleza compleja de estos rasgos. Con el fin de desarrollar una investigación que considere la complejidad del rasgo y, al mismo tiempo, hacer que los experimentos sean más tratables, llevamos a cabo dos estudios. El primero se realizó para investigar la arquitectura genética de la tolerancia al déficit hídrico empleando la Colección de Trabajo del Programa de Mejoramiento de Papa de la Universidad Nacional de Colombia. Se evaluó un panel de diversidad de 104 accesiones diploides de papa bajo tratamientos de riego abundante y de déficit hídrico en la etapa de iniciación del tubérculo. La respuesta a condiciones de déficit hídrico se evaluó con el contenido relativo de clorofila (CC), la eficiencia cuántica máxima de PSII (Fv/Fm), el contenido relativo de agua (RWC), el contenido de azúcar foliar, el número de tubérculos por planta (TN) y el número de tubérculos. peso fresco por planta (PT). Los resultados de la evaluación fenotípica revelaron que las variables fisiológicas, bioquímicas y del componente de rendimiento tuvieron una amplia variación, mientras que las variables del componente de rendimiento distinguieron con mayor fuerza entre los genotipos tolerantes y susceptibles que las variables fisiológicas y bioquímicas. El análisis multivariado basado en el Índice de Tolerancia a la Sequía (DTI) reveló diferentes niveles de tolerancia al déficit hídrico para los 104 genotipos. Los estudios de asociación de todo el genoma (GWAS) se realizaron utilizando una matriz de polimorfismos de un solo nucleótido (SNP) de 47 K, disponible recientemente para esta población. Estamos reportando 38 Quantitative Trait Loci (QTL), siete para condiciones de riego abundante, veintidós para condiciones de déficit hídrico y nueve para DTI que explican entre el 12,6% y el 44,1% de la varianza fenotípica. Se encontró que un conjunto de 6 QTL estaba asociado con más de una variable. De los nueve QTL detectados de DTI en los cromosomas 2, 3, 5, 8, 10 y 12, se identificaron tres genes candidatos con un papel factible en la respuesta al déficit hídrico. Estos resultados proporcionan una base para futuras investigaciones dirigidas a comprender los mecanismos moleculares que subyacen a la tolerancia de la papa a los déficits de agua, y el QTL identificado podría usarse en la selección asistida por marcadores (MAS) para el mejoramiento de la papa con tolerancia al déficit de agua. En el segundo estudio, investigamos las variables fisiológicas, bioquímicas y relacionadas con el rendimiento de cuatro genotipos de papa diploide con tolerancia al déficit de agua que contrastan con el déficit de agua, con énfasis en las respuestas de tendencia temporal. El análisis comparativo identificó con éxito que los cambios fisiológicos fueron más rápidos y pronunciados en los genotipos sensibles al déficit de agua. En cambio, en los genotipos tolerantes el déficit hídrico indujo una acumulación más temprana y notable de sacarosa y glucosa. La respuesta temporal observada mostró que las diferencias estadísticas entre los genotipos tolerantes y sensibles en ψl, CC y contenido de azúcar soluble se pueden distinguir de 3DAT. En general, la variación temporal de los parámetros fisiológicos y bioquímicos demostró la presencia de diferentes estrategias entre los genotipos tolerantes. Estos resultados pueden contribuir a una mejor comprensión de los mecanismos fisiológicos y bioquímicos temporales que conducen a la tolerancia al déficit de agua y ayudarán a los programas de mejoramiento de la papa con una mejor tolerancia al estrés y rendimientos estables en condiciones climáticas cambiantes sin pérdida del potencial de rendimiento en condiciones óptimas. Los resultados de este estudio brindan información sobre la naturaleza de las variaciones genéticas que gobiernan la tolerancia al déficit hídrico en la papa diploide. El conocimiento adquirido de la disección se puede utilizar en programas de mejoramiento para seleccionar líneas con rendimiento mejorado y tolerancia al déficit de agua. Además, la información del panel de polimorfismos SNP y la detección de genes candidatos proporciona vías de investigación para refinar/reducir aún más las regiones genómicas asociadas con estos rasgos agronómicamente importantes, y también abre puertas para la mejora genética a través de la selección genómica asistida. (Texto tomado de la fuente)Colciencias convocatoria doctorado 727DoctoradoDoctor en Ciencias AgrariasModelo de investigacion experimentalgenetica de plantas115 páginasapplication/pdfengUniversidad Nacional de ColombiaBogotá - Ciencias Agrarias - Doctorado en Ciencias AgrariasDepartamento de AgronomíaFacultad de Ciencias AgrariasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura)ALMIDON DE PAPA-ANALISISPotato starch - analysisTolerancewater deficitassociation mappingearly signalToleranciadeficit hidricomapeo por asociacionsenales tempranasNatural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group PhurejaDiversidad alelica natual y senales respuestas asociadas con la tolerancia al deficit hidrico en Solanum tuberosum Grupo PhurejaTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDAgrovocAliche E, Theeuwen T, Oortwijn M, Visser R, Van der Linden G. 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Planta. 2020:251:3. doi: 10.1007/s00425-019-03293-1ColcienciasInvestigadoresORIGINALTesis_PADV.pdfTesis_PADV.pdfTesis de Doctorado en Ciencias Agrariasapplication/pdf3921006https://repositorio.unal.edu.co/bitstream/unal/81580/5/Tesis_PADV.pdf0ec42c38beed6f032bd2c426543e66b2MD55LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81580/6/license.txt8153f7789df02f0a4c9e079953658ab2MD56THUMBNAILTesis_PADV.pdf.jpgTesis_PADV.pdf.jpgGenerated Thumbnailimage/jpeg4958https://repositorio.unal.edu.co/bitstream/unal/81580/7/Tesis_PADV.pdf.jpge1053ebba5c3ce25052bf2b386f84be8MD57unal/81580oai:repositorio.unal.edu.co:unal/815802024-08-06 23:10:08.54Repositorio Institucional Universidad Nacional de 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EVESURBIFBPUiBMQSBTRUNSRVRBUsONQSBHRU5FUkFMLiAqTEEgVEVTSVMgQSBQVUJMSUNBUiBERUJFIFNFUiBMQSBWRVJTScOTTiBGSU5BTCBBUFJPQkFEQS4gCgpBbCBoYWNlciBjbGljIGVuIGVsIHNpZ3VpZW50ZSBib3TDs24sIHVzdGVkIGluZGljYSBxdWUgZXN0w6EgZGUgYWN1ZXJkbyBjb24gZXN0b3MgdMOpcm1pbm9zLiBTaSB0aWVuZSBhbGd1bmEgZHVkYSBzb2JyZSBsYSBsaWNlbmNpYSwgcG9yIGZhdm9yLCBjb250YWN0ZSBjb24gZWwgYWRtaW5pc3RyYWRvciBkZWwgc2lzdGVtYS4KClVOSVZFUlNJREFEIE5BQ0lPTkFMIERFIENPTE9NQklBIC0gw5psdGltYSBtb2RpZmljYWNpw7NuIDE5LzEwLzIwMjEK

Natural allelic diversity and signal responses associated with water deficit tolerance in Solanum tuberosum Group Phureja

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