A new world monkey microsatellite (ap74) higly conserved in primates
Given their great variability, microsatellites or STRPs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blo...
- Autores:
-
Steinberg, Eliana Ruth
Oklander, Luciana Inés
Mudry, Marta Dolores
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2012
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/33000
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/33000
http://bdigital.unal.edu.co/23080/
- Palabra clave:
- 5 Ciencias naturales y matemáticas / Science
59 Animales / Animals
Primates
microsatellite markers
PCR amplification
non-invasive sampling
genomic conservation
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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A new world monkey microsatellite (ap74) higly conserved in primates |
| title |
A new world monkey microsatellite (ap74) higly conserved in primates |
| spellingShingle |
A new world monkey microsatellite (ap74) higly conserved in primates 5 Ciencias naturales y matemáticas / Science 59 Animales / Animals Primates microsatellite markers PCR amplification non-invasive sampling genomic conservation |
| title_short |
A new world monkey microsatellite (ap74) higly conserved in primates |
| title_full |
A new world monkey microsatellite (ap74) higly conserved in primates |
| title_fullStr |
A new world monkey microsatellite (ap74) higly conserved in primates |
| title_full_unstemmed |
A new world monkey microsatellite (ap74) higly conserved in primates |
| title_sort |
A new world monkey microsatellite (ap74) higly conserved in primates |
| dc.creator.fl_str_mv |
Steinberg, Eliana Ruth Oklander, Luciana Inés Mudry, Marta Dolores |
| dc.contributor.author.spa.fl_str_mv |
Steinberg, Eliana Ruth Oklander, Luciana Inés Mudry, Marta Dolores |
| dc.subject.ddc.spa.fl_str_mv |
5 Ciencias naturales y matemáticas / Science 59 Animales / Animals |
| topic |
5 Ciencias naturales y matemáticas / Science 59 Animales / Animals Primates microsatellite markers PCR amplification non-invasive sampling genomic conservation |
| dc.subject.proposal.spa.fl_str_mv |
Primates microsatellite markers PCR amplification non-invasive sampling genomic conservation |
| description |
Given their great variability, microsatellites or STRPs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates. |
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2012 |
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2012-01-01 |
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2019-06-27T22:52:50Z |
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2019-06-27T22:52:50Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Text |
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http://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
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ISSN: 1900-1649 |
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https://repositorio.unal.edu.co/handle/unal/33000 |
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http://bdigital.unal.edu.co/23080/ |
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ISSN: 1900-1649 |
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spa |
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spa |
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http://revistas.unal.edu.co/index.php/actabiol/article/view/19814 |
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Universidad Nacional de Colombia Revistas electrónicas UN Acta Biológica Colombiana Acta Biológica Colombiana |
| dc.relation.references.spa.fl_str_mv |
Steinberg, Eliana Ruth and Oklander, Luciana Inés and Mudry, Marta Dolores (2012) A new world monkey microsatellite (ap74) higly conserved in primates. Acta Biológica Colombiana, 17 (1). pp. 93-102. ISSN 1900-1649 |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biolog�a |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Steinberg, Eliana Ruthf6a2f0b4-e3a7-4f41-96a2-453b0853cab1300Oklander, Luciana Inés8499dc5d-bd47-4aa0-80c5-a46e9dc947a5300Mudry, Marta Dolores95e79c48-4592-4070-8ab6-b623dde902f73002019-06-27T22:52:50Z2019-06-27T22:52:50Z2012-01-01ISSN: 1900-1649https://repositorio.unal.edu.co/handle/unal/33000http://bdigital.unal.edu.co/23080/Given their great variability, microsatellites or STRPs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates.Dado su alta variabilidad, los microsatélites o STR se convirtieron en los marcadores genéticos más ampliamente utilizados en los últimos 15 años. El análisis de estos marcadores requiere una mínima cantidad de ADN, permitiendo el uso de muestras no invasivas, tales como pelos o heces. Con el objetivo de analizar niveles de conservación genómica, amplificamos el microsatélite Ap74 en muestras de pelo y sangre de un amplio rango de primates incluyendo: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., y Homo sapiens. En todos los casos obtuvimos productos de amplificación que exhibieron un tamaño similar (oscilando entre 126 y 176 pb), con excepción del lémur, donde el fragmento detectado presentó un tamaño de aproximadamente 1000 pb. El análisis de las secuencias nucleotídicas nos permitió evaluar las modificaciones moleculares ocurridas durante el proceso evolutivo en primates.application/pdfspaUniversidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biolog�ahttp://revistas.unal.edu.co/index.php/actabiol/article/view/19814Universidad Nacional de Colombia Revistas electrónicas UN Acta Biológica ColombianaActa Biológica ColombianaSteinberg, Eliana Ruth and Oklander, Luciana Inés and Mudry, Marta Dolores (2012) A new world monkey microsatellite (ap74) higly conserved in primates. Acta Biológica Colombiana, 17 (1). pp. 93-102. ISSN 1900-16495 Ciencias naturales y matemáticas / Science59 Animales / AnimalsPrimatesmicrosatellite markersPCR amplificationnon-invasive samplinggenomic conservationA new world monkey microsatellite (ap74) higly conserved in primatesArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL19814-65944-1-SP.jpgimage/jpeg69982https://repositorio.unal.edu.co/bitstream/unal/33000/1/19814-65944-1-SP.jpgeb3ba8da1a4005fd9774b520dc0f72f3MD5119814-65943-1-SP.jpgimage/jpeg316815https://repositorio.unal.edu.co/bitstream/unal/33000/2/19814-65943-1-SP.jpg9666e9309a7cf15758bb053c1dfad1d1MD5219814-65942-1-SP.jpgimage/jpeg180187https://repositorio.unal.edu.co/bitstream/unal/33000/3/19814-65942-1-SP.jpg97831ffb7025faeadb59f31c68212562MD5319814-65945-1-SP.pdfapplication/pdf32071https://repositorio.unal.edu.co/bitstream/unal/33000/4/19814-65945-1-SP.pdff05a14aee6400144c1985abf56a3d1baMD5419814-106840-1-PB.pdfapplication/pdf106897https://repositorio.unal.edu.co/bitstream/unal/33000/5/19814-106840-1-PB.pdfdab6c081eb6a20ebc08c1afec7095e1bMD55THUMBNAIL19814-65945-1-SP.pdf.jpg19814-65945-1-SP.pdf.jpgGenerated Thumbnailimage/jpeg7040https://repositorio.unal.edu.co/bitstream/unal/33000/6/19814-65945-1-SP.pdf.jpgeeeec2401e20453c87d3237ef4e523eeMD5619814-106840-1-PB.pdf.jpg19814-106840-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg6758https://repositorio.unal.edu.co/bitstream/unal/33000/7/19814-106840-1-PB.pdf.jpg25ddc6da165836966c80819d019e46d4MD57unal/33000oai:repositorio.unal.edu.co:unal/330002022-12-24 23:03:50.641Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |