A new world monkey microsatellite (ap74) higly conserved in primates
Given their great variability, microsatellites or STRPs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blo...
- Autores:
-
Steinberg, Eliana Ruth
Oklander, Luciana Inés
Mudry, Marta Dolores
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2012
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/33000
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/33000
http://bdigital.unal.edu.co/23080/
- Palabra clave:
- 5 Ciencias naturales y matemáticas / Science
59 Animales / Animals
Primates
microsatellite markers
PCR amplification
non-invasive sampling
genomic conservation
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
| Summary: | Given their great variability, microsatellites or STRPs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates. |
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