Microbiota y expresión de proteínas intestinales en cerdos adicionados con diferentes antimicrobianos durante el periodo del destete
ilustraciones, diagramas, tablas
- Autores:
-
Herrera Franco, Victor Hugo
- Tipo de recurso:
- Doctoral thesis
- Fecha de publicación:
- 2021
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/81382
- Palabra clave:
- 590 - Animales::599 - Mamíferos
630 - Agricultura y tecnologías relacionadas::636 - Producción animal
Swine - feeding and feeds
Alimentos para cerdos
Producción porcina
Destete
Probiótico
Aceite esencial
Ácido orgánico
Microbiota intestinal
Expresión molecular
Pig production
Weaning
Probiotic
Essential oil
Organic acid
Gut microbiota
Molecular expression
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Microbiota y expresión de proteínas intestinales en cerdos adicionados con diferentes antimicrobianos durante el periodo del destete |
| dc.title.translated.eng.fl_str_mv |
Microbiota and expression of intestinal proteins in pigs added with different antimicrobials during the weaning period |
| title |
Microbiota y expresión de proteínas intestinales en cerdos adicionados con diferentes antimicrobianos durante el periodo del destete |
| spellingShingle |
Microbiota y expresión de proteínas intestinales en cerdos adicionados con diferentes antimicrobianos durante el periodo del destete 590 - Animales::599 - Mamíferos 630 - Agricultura y tecnologías relacionadas::636 - Producción animal Swine - feeding and feeds Alimentos para cerdos Producción porcina Destete Probiótico Aceite esencial Ácido orgánico Microbiota intestinal Expresión molecular Pig production Weaning Probiotic Essential oil Organic acid Gut microbiota Molecular expression |
| title_short |
Microbiota y expresión de proteínas intestinales en cerdos adicionados con diferentes antimicrobianos durante el periodo del destete |
| title_full |
Microbiota y expresión de proteínas intestinales en cerdos adicionados con diferentes antimicrobianos durante el periodo del destete |
| title_fullStr |
Microbiota y expresión de proteínas intestinales en cerdos adicionados con diferentes antimicrobianos durante el periodo del destete |
| title_full_unstemmed |
Microbiota y expresión de proteínas intestinales en cerdos adicionados con diferentes antimicrobianos durante el periodo del destete |
| title_sort |
Microbiota y expresión de proteínas intestinales en cerdos adicionados con diferentes antimicrobianos durante el periodo del destete |
| dc.creator.fl_str_mv |
Herrera Franco, Victor Hugo |
| dc.contributor.advisor.none.fl_str_mv |
Parra Suescún, Jaime Pardo Carrasco, Sandra Clemencia |
| dc.contributor.author.none.fl_str_mv |
Herrera Franco, Victor Hugo |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biodiversidad y Génetica Molecular "Biogem" |
| dc.subject.ddc.spa.fl_str_mv |
590 - Animales::599 - Mamíferos 630 - Agricultura y tecnologías relacionadas::636 - Producción animal |
| topic |
590 - Animales::599 - Mamíferos 630 - Agricultura y tecnologías relacionadas::636 - Producción animal Swine - feeding and feeds Alimentos para cerdos Producción porcina Destete Probiótico Aceite esencial Ácido orgánico Microbiota intestinal Expresión molecular Pig production Weaning Probiotic Essential oil Organic acid Gut microbiota Molecular expression |
| dc.subject.lemb.none.fl_str_mv |
Swine - feeding and feeds Alimentos para cerdos |
| dc.subject.proposal.spa.fl_str_mv |
Producción porcina Destete Probiótico Aceite esencial Ácido orgánico Microbiota intestinal Expresión molecular |
| dc.subject.proposal.eng.fl_str_mv |
Pig production Weaning Probiotic Essential oil Organic acid Gut microbiota Molecular expression |
| description |
ilustraciones, diagramas, tablas |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021-11 |
| dc.date.accessioned.none.fl_str_mv |
2022-03-25T01:17:00Z |
| dc.date.available.none.fl_str_mv |
2022-03-25T01:17:00Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Doctorado |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_db06 |
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Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TD |
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http://purl.org/coar/resource_type/c_db06 |
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acceptedVersion |
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https://repositorio.unal.edu.co/handle/unal/81382 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/81382 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Water Research, 61(15), 243–252. https://doi.org/10.1016/j.watres.2014.05.027 Kim, H. B. & Isaacson, R. E. (2015). The pig gut microbial diversity: Understanding the pig gut microbial ecology through the next generation high throughput sequencing. Veterinary Microbiology, 177(3-4), 242–251. https://doi.org/10.1016/j.vetmic.2015.03.01 Kim, J. C., Hansen, C. F., Mullan, B. P. & Pluske, J. R. (2012). Nutrition and pathology of weaner pigs: Nutritional strategies to support barrier function in the gastrointestinal tract. Animal Feed Science and Technology, 173(1-2), 3–16. https://doi.org/10.1016/j.anifeedsci.2011.12.022 Kim, Y. I., Lee, Y. H., Kim, K. H., Oh, Y. K., Moon, Y. H. & Kwak, W. S. (2012). Effects of supplementing microbially-fermented spent mushroom substrates on growth performance and carcass characteristics of Hanwoo steers (a field study). Asian-Australasian Journal of Animal Sciences, 25(11),1575-1581. https://doi.org/10.5713/ajas.2012.12251 Kogut, M. H. & Arsenault, R. J. 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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Parra Suescún, Jaime1a28c416ea72f10986f6872e5a91ab08600Pardo Carrasco, Sandra Clemenciafe39f49924d8437bd04909027b444124600Herrera Franco, Victor Hugo947965d163124c7700388dcf3a12f181600Biodiversidad y Génetica Molecular "Biogem"2022-03-25T01:17:00Z2022-03-25T01:17:00Z2021-11https://repositorio.unal.edu.co/handle/unal/81382Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, tablasEl destete es el período más estresante de la vida del cerdo, debido principalmente a que se encuentra en desafío con diferentes cambios (ambientales/sociales/nutricionales) a los que debe adaptarse rápidamente. Durante el destete precoz (≤ 21 días), el lechón se encuentra en medio de su desarrollo fisiológico y establecimiento de la microbiota intestinal, a su vez, la inmunidad alcanzada con la leche materna puede disminuir, lo que representa un reto para las múltiples funciones del intestino animal. El epitelio intestinal facilita la separación del medio exterior con el medio interno, la descomposición y absorción de los nutrientes de los alimentos, la defensa contra agentes no propios, y representa el ambiente de los microorganismos responsables de una relación simbiótica con el animal. Para contrarrestar los efectos perjudiciales del destete han sido utilizados los antibióticos, al tiempo que cumplen la función de promotores de crecimiento, aumentando el rendimiento productivo y la eficiencia alimenticia en cerdos en crecimiento. Debido a que se han considerado como factores de transmisión de resistencias a los microorganismos contra antibióticos utilizados en medicina humana, se ha creado controversia acerca del uso de cantidades subterapéuticas de dichos antibióticos en la producción porcina intensiva. Por lo anterior, los objetivo de este estudio fueron evaluar el desarrollo productivo de los lechones; cuantificar la abundancia de ARNm de los genes de enzimas (maltasa-glucoamilasa (MgA) y sacarasa-isomaltasa (SI)), transportadores (SGLT-1, GLUT-1 y GLUT-2) y citoquinas intestinales; y caracterizar la microbiota en el intestino (yeyuno) en cerdos destetados a dos edades y alimentados con la adición de antibióticos promotores de crecimiento (APC) y diferentes alternativas a estos, como probióticos (Bacillus subtilis), aceites esenciales (de orégano [Lippia origanoides]) y ácidos orgánicos. La adición de alternativas a los APC, especialmente probióticos, en fase de crecimiento, junto con el destete a los 28 días de edad (tardío), evidenció un impacto favorable, no solo en la función digestiva y de protección intestinal de cerdos, sino también en el establecimiento rápido y beneficioso de la microbiota intestinal, influyendo sobre los parámetros productivos, y sirviendo de base para el análisis de resultados moleculares relacionados con la salud intestinal. Además, esta investigación proporciona una base para el uso de alternativas naturales a los antibióticos que pueden promover el desarrollo de los cerdos en crecimiento, y favorecer la adaptación del lechón a la fase posdestete. (Texto tomado de la fuente)Weaning is the most stressful period in the life of the pig, mainly because of environmental / social / nutritional it is in challenge with different changes, (environmental / social / nutritional) to which it must adapt quickly. In early weaning (≤ 21 days), the piglet is in the middle of its physiological development and establishment of the intestinal microbiota, and the immunity achieved with breast milk decreases, which represents a challenge for the multiple functions of the animal intestine. The intestinal epithelium facilitates the separation of the external environment from the internal environment, the decomposition and absorption of nutrients from food, the defense against non-self agents, and represents the environment of the microorganisms responsible for a symbiotic relationship with the animal. Antibiotics have been used to counteract the harmful effects of weaning, while they play the role of acting as growth promoters, increase increasing productive performance and feed efficiency in growing pigs. Since they have been considered as transmission factors of resistance to microorganisms against antibiotics used in human medicine, controversy has been created raised about the use of subtherapeutic amounts of these antibiotics in intensive swine production. Therefore, the objectives of this study were to evaluate the productive development of the piglets; quantify the abundance of mRNA of the genes of coding for digestive enzymes (maltase-glucoamylase (MgA) and sucrase-isomaltase (SI)), transporters (SGLT-1, GLUT-1 and GLUT-2) and intestinal cytokines via mRNA; and characterize the microbiota via 16SrRNA MiSeq sequencing in the intestine (jejunum) in pigs weaned at two ages and fed with the addition of growth-promoting antibiotics (APC) and different alternatives to these, such as probiotics (Bacillus subtilis), essential oils (from oregano [Lippia origanoides)]) and organic acids. The addition of alternatives to APC, especially probiotics, in the growth phase, together with weaning at 28 days of age (late), showed a favorable impact, not only on the digestive function and intestinal protection of pigs, but also in the rapid and beneficial establishment of the intestinal microbiota, influencing the productive parameters, and serving as the basis for the analysis of molecular results related to intestinal health. In addition, this research provides a basis for the use of natural alternatives to antibiotics that can promote the development of growing pigs and favor the adaptation of the piglet to the post-weaning phase.Ministerio de Ciencia, Tecnología e Innovación (anteriormente conocido como "COLCIENCIAS")DoctoradoDoctor en BiotecnologíaBiotecnología aplicada a la producción animal: microbioma intestinalÁrea Curricular Biotecnologíaxvi, 126 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Doctorado en BiotecnologíaEscuela de biocienciasFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín590 - Animales::599 - Mamíferos630 - Agricultura y tecnologías relacionadas::636 - Producción animalSwine - feeding and feedsAlimentos para cerdosProducción porcinaDesteteProbióticoAceite esencialÁcido orgánicoMicrobiota intestinalExpresión molecularPig productionWeaningProbioticEssential oilOrganic acidGut microbiotaMolecular expressionMicrobiota y expresión de proteínas intestinales en cerdos adicionados con diferentes antimicrobianos durante el periodo del desteteMicrobiota and expression of intestinal proteins in pigs added with different antimicrobials during the weaning periodTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDAlfonso, K. 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Poultry Science, 97(8), 2654–2666. https://doi.org/10.3382/ps/pey119EstudiantesInvestigadoresMaestrosORIGINAL1020418193.2021.pdf1020418193.2021.pdfTesis de Doctorado en Biotecnologíaapplication/pdf1597654https://repositorio.unal.edu.co/bitstream/unal/81382/3/1020418193.2021.pdf0ddc155d1617760cfb7bfd57070d161bMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81382/4/license.txt8153f7789df02f0a4c9e079953658ab2MD54THUMBNAIL1020418193.2021.pdf.jpg1020418193.2021.pdf.jpgGenerated Thumbnailimage/jpeg3824https://repositorio.unal.edu.co/bitstream/unal/81382/5/1020418193.2021.pdf.jpgeade10f0b7a0012343d7455d9bed8987MD55unal/81382oai:repositorio.unal.edu.co:unal/813822024-08-05 23:10:21.866Repositorio Institucional Universidad Nacional de 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