Evaluación In vitro de la actividad antimicrobiana de las células estromales mesenquimales de gelatina de Wharton (CEM-GW)

Antimicrobial resistance is a growing problem in global public health and threatens the prevention and effective treatment of infections. The investigation of alternative strategies point to the generation and use of antimicrobial peptides (AMP) given its broad spectrum of antimicrobial activity. A...

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Autores:
Méndez Báez, Leidi Yohana
Tipo de recurso:
Work document
Fecha de publicación:
2020
Institución:
Universidad Nacional de Colombia
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Universidad Nacional de Colombia
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spa
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oai:repositorio.unal.edu.co:unal/77872
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/77872
Palabra clave:
570 - Ciencias de la vida
610 - Medicina y salud
péptidos antimicrobianos
β-defensina-1
lipocalina-2
CEM-GW
hepcidina
cepas ATCC
antimicrobial peptides
β-defensin-1
lipocalin-2
hepcidin
CEM-GW
ATCC strains
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openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_ee826c6d037eb73ab3db9a915bb5fb87
oai_identifier_str oai:repositorio.unal.edu.co:unal/77872
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Evaluación In vitro de la actividad antimicrobiana de las células estromales mesenquimales de gelatina de Wharton (CEM-GW)
title Evaluación In vitro de la actividad antimicrobiana de las células estromales mesenquimales de gelatina de Wharton (CEM-GW)
spellingShingle Evaluación In vitro de la actividad antimicrobiana de las células estromales mesenquimales de gelatina de Wharton (CEM-GW)
570 - Ciencias de la vida
610 - Medicina y salud
péptidos antimicrobianos
β-defensina-1
lipocalina-2
CEM-GW
hepcidina
cepas ATCC
antimicrobial peptides
β-defensin-1
lipocalin-2
hepcidin
CEM-GW
ATCC strains
title_short Evaluación In vitro de la actividad antimicrobiana de las células estromales mesenquimales de gelatina de Wharton (CEM-GW)
title_full Evaluación In vitro de la actividad antimicrobiana de las células estromales mesenquimales de gelatina de Wharton (CEM-GW)
title_fullStr Evaluación In vitro de la actividad antimicrobiana de las células estromales mesenquimales de gelatina de Wharton (CEM-GW)
title_full_unstemmed Evaluación In vitro de la actividad antimicrobiana de las células estromales mesenquimales de gelatina de Wharton (CEM-GW)
title_sort Evaluación In vitro de la actividad antimicrobiana de las células estromales mesenquimales de gelatina de Wharton (CEM-GW)
dc.creator.fl_str_mv Méndez Báez, Leidi Yohana
dc.contributor.advisor.spa.fl_str_mv Salguero López, Gustavo Andres
Lozano Moreno, José Manuel
dc.contributor.author.spa.fl_str_mv Méndez Báez, Leidi Yohana
dc.contributor.corporatename.spa.fl_str_mv Instituto Distrital de Ciencia Biotecnología e Innovación en Salud IDCBIS
dc.contributor.researchgroup.spa.fl_str_mv Unidad de Terapias Avanzadas - IDCBIS
dc.subject.ddc.spa.fl_str_mv 570 - Ciencias de la vida
610 - Medicina y salud
topic 570 - Ciencias de la vida
610 - Medicina y salud
péptidos antimicrobianos
β-defensina-1
lipocalina-2
CEM-GW
hepcidina
cepas ATCC
antimicrobial peptides
β-defensin-1
lipocalin-2
hepcidin
CEM-GW
ATCC strains
dc.subject.proposal.spa.fl_str_mv péptidos antimicrobianos
β-defensina-1
lipocalina-2
CEM-GW
hepcidina
cepas ATCC
dc.subject.proposal.eng.fl_str_mv antimicrobial peptides
β-defensin-1
lipocalin-2
hepcidin
CEM-GW
ATCC strains
description Antimicrobial resistance is a growing problem in global public health and threatens the prevention and effective treatment of infections. The investigation of alternative strategies point to the generation and use of antimicrobial peptides (AMP) given its broad spectrum of antimicrobial activity. A potential source of (AMP) resides in human mesenchymal stromal cells (MSC). MSC have demonstrated potent therapeutic effects in terms of immunomodulation and multilineage differentiation and are actively involved in tissue control and repair. This work focused on exploring the potential antimicrobial effect of MSC isolated from Wharton's jelly (GW) of the umbilical cord upon experimental infection with bacterial strains Escherichia coli 25922, Klebsiella pneumoniae 43816, Staphylococcus aureus 29213 and Staphylococcus epidermidis 12228. Experimental bacterial infection on CEM-GW triggered important antimicrobial activity whose magnitude depended on the inoculated bacterial strain. This observed antimicrobial effect depended strongly on the presence of human platelet lysate (LPh) in MSC growth media. Importantly, based on characterization of RNA expression and secretion of the AMPs β-defensin-1, Lipocalin-2 and Hepcidin in supernatant, the antimicrobial effect of CEM-GW was significantly associated with increased expression and secretion of AMPs, especially β-defensin-1 and Lipocalin-2. These results provide evidence of the antimicrobial effect that CEM-GW exerts on the bacterial strains used, which depends on the presence of LPh and results in the expression and secretion of AMPs, as a potential mechanism of defense against infection.
publishDate 2020
dc.date.accessioned.spa.fl_str_mv 2020-07-29T16:11:30Z
dc.date.available.spa.fl_str_mv 2020-07-29T16:11:30Z
dc.date.issued.spa.fl_str_mv 2020-05-01
dc.type.spa.fl_str_mv Documento de trabajo
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/workingPaper
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_8042
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/WP
format http://purl.org/coar/resource_type/c_8042
status_str acceptedVersion
dc.identifier.citation.spa.fl_str_mv Evaluación In vitro de la actividad antimicrobiana de las Células Estromales Mesenquimales de Gelatina de Wharton (CEM-GW)
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/77872
identifier_str_mv Evaluación In vitro de la actividad antimicrobiana de las Células Estromales Mesenquimales de Gelatina de Wharton (CEM-GW)
url https://repositorio.unal.edu.co/handle/unal/77872
dc.language.iso.spa.fl_str_mv spa
language spa
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6. Klimczak, A. and U. Kozlowska, Mesenchymal Stromal Cells and Tissue-Specific Progenitor Cells: Their Role in Tissue Homeostasis. Stem Cells Int, 2016. 2016: p. 4285215.
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dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.spa.spa.fl_str_mv Acceso abierto
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
Acceso abierto
http://creativecommons.org/licenses/by-nc/4.0/
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eu_rights_str_mv openAccess
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dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de ColombiaAcceso abiertohttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Salguero López, Gustavo Andresb4f169ab-6902-4958-87fc-00b4ef49a7e0-1Lozano Moreno, José Manuel677e0455-15ed-4bbc-95fa-d04944c6d4cc-1Méndez Báez, Leidi Yohanaa413086b-b317-4238-95f7-18f1e55d3fdaInstituto Distrital de Ciencia Biotecnología e Innovación en Salud IDCBISUnidad de Terapias Avanzadas - IDCBIS2020-07-29T16:11:30Z2020-07-29T16:11:30Z2020-05-01Evaluación In vitro de la actividad antimicrobiana de las Células Estromales Mesenquimales de Gelatina de Wharton (CEM-GW)https://repositorio.unal.edu.co/handle/unal/77872Antimicrobial resistance is a growing problem in global public health and threatens the prevention and effective treatment of infections. The investigation of alternative strategies point to the generation and use of antimicrobial peptides (AMP) given its broad spectrum of antimicrobial activity. A potential source of (AMP) resides in human mesenchymal stromal cells (MSC). MSC have demonstrated potent therapeutic effects in terms of immunomodulation and multilineage differentiation and are actively involved in tissue control and repair. This work focused on exploring the potential antimicrobial effect of MSC isolated from Wharton's jelly (GW) of the umbilical cord upon experimental infection with bacterial strains Escherichia coli 25922, Klebsiella pneumoniae 43816, Staphylococcus aureus 29213 and Staphylococcus epidermidis 12228. Experimental bacterial infection on CEM-GW triggered important antimicrobial activity whose magnitude depended on the inoculated bacterial strain. This observed antimicrobial effect depended strongly on the presence of human platelet lysate (LPh) in MSC growth media. Importantly, based on characterization of RNA expression and secretion of the AMPs β-defensin-1, Lipocalin-2 and Hepcidin in supernatant, the antimicrobial effect of CEM-GW was significantly associated with increased expression and secretion of AMPs, especially β-defensin-1 and Lipocalin-2. These results provide evidence of the antimicrobial effect that CEM-GW exerts on the bacterial strains used, which depends on the presence of LPh and results in the expression and secretion of AMPs, as a potential mechanism of defense against infection.La resistencia a los antimicrobianos es un problema creciente de salud pública mundial y amenaza la prevención y el tratamiento eficaz de las infecciones. La investigación de estrategias alternativas apunta a la generación y uso de péptidos antimicrobianos (AMP) dado su amplio espectro de actividad antimicrobiana. Una potencial fuente de (AMP) reside en células estromales mesenquimales (CEM) humanas. Las CEM han demostrado potentes efectos terapéuticos en términos de inmunomodulación y diferenciación multilinaje y participan activamente en el control y reparación tisular. Este trabajo se enfocó en explorar el potencial efecto antimicrobiano de las CEM aisladas de gelatina de Wharton (GW) del cordón umbilical ante la infección experimental con las cepas bacterianas Escherichia coli 25922, Klebsiella pneumoniae 43816, Staphylococcus aureus 29213 y Staphylococcus epidermidis 12228. A partir de la exposición de inóculos bacterianos a CEM-GW se pudo observar una importante actividad antimicrobiana cuya magnitud dependió de la cepa bacteriana inoculada. Este efecto antimicrobiano observado se relacionó fuertemente con la presencia de lisado plaquetario humano (LPh). De manera importante a partir de la caracterización de la expresión de ARN mensajero y secreción de los AMPs β-defensina-1, Lipocalina-2 y Hepcidina en sobrenadante, el efecto antimicrobiano de las CEM-GW se asoció significativamente al incremento de la expresión y secreción de AMPs, especialmente β-defensina-1 y Lipocalina-2. Estos resultados proveen evidencia del efecto antimicrobiano que ejercen las CEM-GW sobre las cepas bacterianas utilizadas, el cual depende de la presencia de LPh y deriva en la expresión y secreción de AMPs, como mecanismo de defensa a la infección.Instituto DIsitrital de Ciencia, Biotecnología e Innovación en Salud (IDCBIS)Evaluación In vitro de la actividad antimicrobiana de las Células Estromales Mesenquimales de Gelatina de Wharton (CEM-GW)Magíster en Ciencias Microbiología.Maestría111application/pdfspa570 - Ciencias de la vida610 - Medicina y saludpéptidos antimicrobianosβ-defensina-1lipocalina-2CEM-GWhepcidinacepas ATCCantimicrobial peptidesβ-defensin-1lipocalin-2hepcidinCEM-GWATCC strainsEvaluación In vitro de la actividad antimicrobiana de las células estromales mesenquimales de gelatina de Wharton (CEM-GW)Documento de trabajoinfo:eu-repo/semantics/workingPaperinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_8042Texthttp://purl.org/redcol/resource_type/WPBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaUniversidad Nacional de Colombia - Sede Bogotá1. OMS, Organización Mundial de la Salud, OMS, Editor. 2019.2. OMS, Informe sobre la salud del mundo 2017. Ginebra. 2017, Organización Mundial de la Salud3. Tabares, V.R. Resistencia bacteriana en la mira de investigadores. 2016 [cited 2018.4. González García, M., et al., Péptidos antimicrobianos: potencialidades terapéuticas. 2017. Vol. 69. 2017.5. Meisel, R., et al., Human but not murine multipotent mesenchymal stromal cells exhibit broad-spectrum antimicrobial effector function mediated by indoleamine 2, 3-dioxygenase. Leukemia, 2011. 25(4): p. 648.6. Klimczak, A. and U. Kozlowska, Mesenchymal Stromal Cells and Tissue-Specific Progenitor Cells: Their Role in Tissue Homeostasis. Stem Cells Int, 2016. 2016: p. 4285215.7. da Silva Meirelles, L., P.C. Chagastelles, and N.B. Nardi, Mesenchymal stem cells reside in virtually all post-natal organs and tissues. J Cell Sci, 2006. 119(Pt 11): p. 2204-13.8. 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Stem Cell Res Ther, 2018. 9(1): p. 124.CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8701https://repositorio.unal.edu.co/bitstream/unal/77872/5/license_rdf42fd4ad1e89814f5e4a476b409eb708cMD55LICENSElicense.txtlicense.txttext/plain; charset=utf-83991https://repositorio.unal.edu.co/bitstream/unal/77872/7/license.txt6f3f13b02594d02ad110b3ad534cd5dfMD57ORIGINAL53040841.2020.pdf53040841.2020.pdfapplication/pdf4525766https://repositorio.unal.edu.co/bitstream/unal/77872/6/53040841.2020.pdfbd91bb1d040cb742380215c459da7a64MD56THUMBNAIL53040841.2020.pdf.jpg53040841.2020.pdf.jpgGenerated Thumbnailimage/jpeg5382https://repositorio.unal.edu.co/bitstream/unal/77872/8/53040841.2020.pdf.jpgefc441d8e7265e451e40c58e0280f743MD58unal/77872oai:repositorio.unal.edu.co:unal/778722024-07-19 23:33:03.67Repositorio Institucional Universidad Nacional de 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W5pbyBww7pibGljby4gU2kgbm8gZnVlc2UgZWwgY2FzbywgYWNlcHRvIHRvZGEgbGEgcmVzcG9uc2FiaWxpZGFkIHBvciBjdWFscXVpZXIgaW5mcmFjY2nDs24gZGUgZGVyZWNob3MgZGUgYXV0b3IgcXVlIGNvbmxsZXZlIGxhIGRpc3RyaWJ1Y2nDs24gZGUgZXN0b3MgYXJjaGl2b3MgeSBtZXRhZGF0b3MuCkFsIGhhY2VyIGNsaWMgZW4gZWwgc2lndWllbnRlIGJvdMOzbiwgdXN0ZWQgaW5kaWNhIHF1ZSBlc3TDoSBkZSBhY3VlcmRvIGNvbiBlc3RvcyB0w6lybWlub3MuCg==

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