Evaluación de la capacidad de adhesión de cepas bacterianas con propiedades probióticas en líneas celulares humanas tumorales de colon
ilustraciones., diagramas
- Autores:
-
Roldán Pérez, Samantha
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/82661
- Palabra clave:
- 570 - Biología
610 - Medicina y salud
570 - Biología::572 - Bioquímica
Bacterias
Bacteria
Bacterias probióticas
Bacterias Ácido Lácticas
Epitelio intestinal
Capa paracristalina
Adhesinas
Lactic Acid Bacteria
Intestinal epithelium
S-layer
Adhesins
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Evaluación de la capacidad de adhesión de cepas bacterianas con propiedades probióticas en líneas celulares humanas tumorales de colon |
| dc.title.translated.eng.fl_str_mv |
Evaluation of adhesion capacity of bacterial strains with probiotic properties in human tumoral colonic cell lines |
| title |
Evaluación de la capacidad de adhesión de cepas bacterianas con propiedades probióticas en líneas celulares humanas tumorales de colon |
| spellingShingle |
Evaluación de la capacidad de adhesión de cepas bacterianas con propiedades probióticas en líneas celulares humanas tumorales de colon 570 - Biología 610 - Medicina y salud 570 - Biología::572 - Bioquímica Bacterias Bacteria Bacterias probióticas Bacterias Ácido Lácticas Epitelio intestinal Capa paracristalina Adhesinas Lactic Acid Bacteria Intestinal epithelium S-layer Adhesins |
| title_short |
Evaluación de la capacidad de adhesión de cepas bacterianas con propiedades probióticas en líneas celulares humanas tumorales de colon |
| title_full |
Evaluación de la capacidad de adhesión de cepas bacterianas con propiedades probióticas en líneas celulares humanas tumorales de colon |
| title_fullStr |
Evaluación de la capacidad de adhesión de cepas bacterianas con propiedades probióticas en líneas celulares humanas tumorales de colon |
| title_full_unstemmed |
Evaluación de la capacidad de adhesión de cepas bacterianas con propiedades probióticas en líneas celulares humanas tumorales de colon |
| title_sort |
Evaluación de la capacidad de adhesión de cepas bacterianas con propiedades probióticas en líneas celulares humanas tumorales de colon |
| dc.creator.fl_str_mv |
Roldán Pérez, Samantha |
| dc.contributor.advisor.none.fl_str_mv |
Márquez Fernández, María Elena Montoya Campuzano, Olga Inés |
| dc.contributor.author.none.fl_str_mv |
Roldán Pérez, Samantha |
| dc.contributor.researchgroup.spa.fl_str_mv |
Probióticos: Prospección Funcional y Metabolitos Grupo de Investigación en Biotecnología Animal (Giba) |
| dc.contributor.orcid.spa.fl_str_mv |
Márquez Fernández, María Elena [0000-0001-5760-9907] |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología 610 - Medicina y salud 570 - Biología::572 - Bioquímica |
| topic |
570 - Biología 610 - Medicina y salud 570 - Biología::572 - Bioquímica Bacterias Bacteria Bacterias probióticas Bacterias Ácido Lácticas Epitelio intestinal Capa paracristalina Adhesinas Lactic Acid Bacteria Intestinal epithelium S-layer Adhesins |
| dc.subject.mesh.spa.fl_str_mv |
Bacterias |
| dc.subject.mesh.eng.fl_str_mv |
Bacteria |
| dc.subject.other.spa.fl_str_mv |
Bacterias probióticas |
| dc.subject.proposal.spa.fl_str_mv |
Bacterias Ácido Lácticas Epitelio intestinal Capa paracristalina Adhesinas |
| dc.subject.proposal.eng.fl_str_mv |
Lactic Acid Bacteria Intestinal epithelium S-layer Adhesins |
| description |
ilustraciones., diagramas |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-11-08T16:26:56Z |
| dc.date.available.none.fl_str_mv |
2022-11-08T16:26:56Z |
| dc.date.issued.none.fl_str_mv |
2022-11 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
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http://purl.org/redcol/resource_type/TM |
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acceptedVersion |
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https://repositorio.unal.edu.co/handle/unal/82661 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/82661 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
| language |
spa |
| dc.relation.indexed.spa.fl_str_mv |
RedCol LaReferencia |
| dc.relation.references.spa.fl_str_mv |
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Universidad Nacional de Colombia |
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Medellín - Ciencias - Maestría en Ciencias - Biotecnología |
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Facultad de Ciencias |
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Universidad Nacional de Colombia - Sede Medellín |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Márquez Fernández, María Elena6dffb26b95b62c41dad1e992612500d3600Montoya Campuzano, Olga Inés13b1087695f9ed0342ccae3e2ccd10be600Roldán Pérez, Samantha43786bffbe2d5e5b354e40a6fa75b7da600Probióticos: Prospección Funcional y MetabolitosGrupo de Investigación en Biotecnología Animal (Giba)Márquez Fernández, María Elena [0000-0001-5760-9907]2022-11-08T16:26:56Z2022-11-08T16:26:56Z2022-11https://repositorio.unal.edu.co/handle/unal/82661Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones., diagramasLas Bacterias Ácido Lácticas (BAL) son productoras de ácido láctico, reconocidas como probióticas seguras, que administradas en cantidades adecuadas confieren un beneficio para la salud del hospedero. Las BAL pueden aislarse de derivados lácteos artesanales, como el Queso Doble Crema (QDC). El objetivo de esta investigación fue evaluar la capacidad de adhesión de las cepas Pediococcus pentosaceus, Weissella viridescens y Lacticaseibacillus casei con propiedades probióticas en líneas celulares humanas tumorales de colon SW480 y SW620. A estas cepas BAL aisladas y caracterizadas previamente de un QDC, se le evaluaron algunas de sus propiedades probióticas como la resistencia a diferentes condiciones de pH y de sales biliares, sensibilidad a los antibióticos, actividad antibacteriana, capacidad de autoagregación, producción de exopolisacáridos y adhesión a líneas celulares humanas tumorales de colon SW480 y SW620. Todas las cepas BAL-QDC sobrevivieron en condiciones de pH (5.5, 6.5 y 8.0) y a sales biliares (0.3%, 0.6% y 1.0% p/v); además, inhibieron el crecimiento de E. coli ATCC 25922, S. Typhimurium ATCC 14028, S. aureus ATCC 25923 y L. monocytogenes, excepto W. viridescens que no inhibió a L. monocytogenes. Las BAL-QDC P. pentosaceus, W. viridescens y L. casei no mostraron resistencia a los antibióticos Gentamicina, Penicilina, Vancomicina, Ampicilina, Tetraciclina y Cloranfenicol, pero W. viridescens exhibió resistencia al cloranfenicol. Las BAL-QDC también presentaron capacidad de autoagregación y porcentajes de adhesión superiores al 54.97% a las células SW480 y SW620 respecto a L. rhamnosus GG. Las proteínas de la capa paracristalina de las BAL-QDC afectaron únicamente la adhesión de L. casei en las células SW620 y a L. rhamnosus GG con las células SW480. (Texto tomado de la fuente)Lactic Acid Bacteria (LAB) are producers of lactic acid, recognized as safe probiotics, which when administered in adequate amounts confer a health benefit on the host. BAL can be isolated from artisan dairy products, such as Double Cream Cheese (DCC). The aim of this research was to evaluate the adhesion capacity of the strains Pediococcus pentosaceus, Weissella viridescens and Lacticaseibacillus casei with probiotic properties in human colonic tumor cell lines SW480 and SW620. These LAB strains previously isolated and characterized from a DCC were evaluated for some of their probiotic properties such as resistance to different pH and bile salt conditions, sensitivity to antibiotics, antibacterial activity, autoaggregation capacity, production of exopolysaccharides and adhesion to human colonic tumor cell lines SW480 and SW620. All LAB-DCC strains survived under conditions of pH (5.5, 6.5 and 8.0) and bile salts (0.3%, 0.6% and 1.0% w/v); In addition, they inhibited the growth of E. coli ATCC 25922, S. Typhimurium ATCC 14028, S. aureus ATCC 25923, and L. monocytogenes, except for W. viridescens, which did not inhibit L. monocytogenes. LAB-DCC P. pentosaceus, W. viridescens and L. casei did not show resistance to the antibiotics Gentamicin, Penicillin, Vancomycin, Ampicillin, Tetracycline and Chloramphenicol, but W. viridescens showed resistance to chloramphenicol. LAB-DCC also showed autoaggregation capacity and adhesion percentages higher than 54.965% to SW480 and SW620 cells relative to L. rhamnosus GG. LAB-DCC S-layer proteins affected only the adhesion of L. casei to SW620 cells and L. rhamnosus GG to SW480 cells.MaestríaMagíster en Ciencias - BiotecnologíaProbióticosÁrea Curricular de Bioctecnología104 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín570 - Biología610 - Medicina y salud570 - Biología::572 - BioquímicaBacteriasBacteriaBacterias probióticasBacterias Ácido LácticasEpitelio intestinalCapa paracristalinaAdhesinasLactic Acid BacteriaIntestinal epitheliumS-layerAdhesinsEvaluación de la capacidad de adhesión de cepas bacterianas con propiedades probióticas en líneas celulares humanas tumorales de colonEvaluation of adhesion capacity of bacterial strains with probiotic properties in human tumoral colonic cell linesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMRedColLaReferenciaAbraham, B. 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In vitroassessment of the probiotic properties and bacteriocinogenic potential of pediococcus pentosaceusMZF16 isolated from artisanal tunisian meat "dried ossban. Frontiers in Microbiology, 9(NOV), 2607. https://doi.org/10.3389/fmicb.2018.02607EstudiantesInvestigadoresMaestrosORIGINAL1152214584.2022.pdf1152214584.2022.pdfTesis de Maestría en Ciencias - Biotecnologíaapplication/pdf1562236https://repositorio.unal.edu.co/bitstream/unal/82661/4/1152214584.2022.pdff310f358a1d0ed74a46757bd0f64a256MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/82661/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53THUMBNAIL1152214584.2022.pdf.jpg1152214584.2022.pdf.jpgGenerated Thumbnailimage/jpeg4652https://repositorio.unal.edu.co/bitstream/unal/82661/5/1152214584.2022.pdf.jpg8b8bed1383faab3b1971f68979e63af7MD55unal/82661oai:repositorio.unal.edu.co:unal/826612024-08-12 23:11:23.973Repositorio Institucional Universidad Nacional de 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