Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno.

Ilustraciones, tablas, gráficas

Autores:: Martínez López, Luisa María

Tipo de recurso:

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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repository_id_str
dc.title.spa.fl_str_mv	Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno.
dc.title.translated.spa.fl_str_mv	Study of the intestinal microbiome of Zophobas morio (Coleoptera: Tenebrionidae) as an approach to the potential use for the biodegradation of polystyrene.
title	Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno.
spellingShingle	Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno. 570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales Microbiomas Microbiomes Diversidad microbiana Microbial diversity Actividad enzimática Enzyme activity Tenebrionidae EPS Metabarcoding Poliestireno Metagenómica Biodegradación Coleópteros EPS Metabarcoding Coleoptera Metagenomics Biodegradation Polystyrene
title_short	Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno.
title_full	Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno.
title_fullStr	Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno.
title_full_unstemmed	Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno.
title_sort	Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno.
dc.creator.fl_str_mv	Martínez López, Luisa María
dc.contributor.advisor.none.fl_str_mv	López Álvarez, Diana Carolina Rojas Triviño, Edwison Alberto
dc.contributor.author.none.fl_str_mv	Martínez López, Luisa María
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Investigación en Diversidad Biológica
dc.subject.ddc.spa.fl_str_mv	570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales
topic	570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales Microbiomas Microbiomes Diversidad microbiana Microbial diversity Actividad enzimática Enzyme activity Tenebrionidae EPS Metabarcoding Poliestireno Metagenómica Biodegradación Coleópteros EPS Metabarcoding Coleoptera Metagenomics Biodegradation Polystyrene
dc.subject.agrovoc.none.fl_str_mv	Microbiomas Microbiomes Diversidad microbiana Microbial diversity Actividad enzimática Enzyme activity Tenebrionidae
dc.subject.proposal.spa.fl_str_mv	EPS Metabarcoding Poliestireno Metagenómica Biodegradación Coleópteros
dc.subject.proposal.eng.fl_str_mv	EPS Metabarcoding Coleoptera Metagenomics Biodegradation Polystyrene
description	Ilustraciones, tablas, gráficas
publishDate	2023
dc.date.issued.none.fl_str_mv	2023
dc.date.accessioned.none.fl_str_mv	2024-01-30T20:58:37Z
dc.date.available.none.fl_str_mv	2024-01-30T20:58:37Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/85544
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/85544 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Palmira - Ingeniería y Administración - Maestría en Ingeniería - Ingeniería Ambiental
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ingeniería y Administración
dc.publisher.place.spa.fl_str_mv	Palmira, Valle del Cauca, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Palmira
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2López Álvarez, Diana Carolina1ab298e9492bfd0c4b69bb243416b257Rojas Triviño, Edwison Alberto99ee15dff8b1405b92a81050bb73569cMartínez López, Luisa María4e6d2322b953004664a5cdf471ca3c71Grupo de Investigación en Diversidad Biológica2024-01-30T20:58:37Z2024-01-30T20:58:37Z2023https://repositorio.unal.edu.co/handle/unal/85544Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/Ilustraciones, tablas, gráficasEl Poliestireno expandido (EPS) es un polímero sintético de uso común en diferentes industrias, principalmente en las de embalaje y construcción. Debido a su composición química a base de cadenas largas de hidrocarburos, el EPS posee una estabilidad molecular que le permite permanecer inmodificable por cientos de años lo que genera un impacto negativo sobre el medio ambiente, ya que no es un sustrato nutritivo para ningún microorganismo o se desconocía para cuál. En la actualidad, se ha reportado que larvas de coleópteros de la familia Tenebrionidae pueden biodegradarlo gracias a enzimas producidas por su microbiota intestinal. Considerando lo anterior, el objetivo de esta investigación fue caracterizar la microbiota intestinal de larvas de Zophobas morio como aproximación al uso potencial para la biodegradación del Poliestireno (PS), empleando metabarcoding. Para esto se realizó secuenciación Illumina Miseq de la región V3-4 del gen 16s del ARNr de larvas de Z. morio alimentadas con dos tipos de dietas, EPS y Avena. Adicionalmente, a través de la plataforma de secuenciación Hiseq2500 se obtuvo el metagenoma completo de una de las muestras alimentadas con EPS. Con los resultados de la secuenciación se analizó la composición taxonómica, abundancia relativa de las bacterias y finalmente se realizó un análisis metabólico del microbioma obtenido de las larvas alimentadas con EPS. El análisis metagenómico permitió identificar que el género Spiroplasma presentó una abundancia relativa de 61,6 %, seguido de Pantoea (19,3%), Enterobacter (5%) y Pseudarthrobacter (4,7%). Otros géneros presentes fueron Kluyvera georgiana, Enterococcus sp., Bacillus sp., Clostridium sp., y Romboutsia sp. Se asume a estos géneros como los microorganismos más promisorios que influyen en la biodegradación del EPS. Los resultados obtenidos podrían ser incluidos en trabajos futuros donde se analice la actividad biológica y de biodegradación de microorganismos pertenecientes a los géneros encontrados. (Texto tomadode la fuente)Expanded Polystyrene (EPS) is a synthetic polymer commonly used in different industries, mainly in packaging and construction. Due to its chemical composition based on long hydrocarbon chains, EPS has a molecular stability that allows it to remain unchanged for hundreds of years, which generates a negative impact on the environment, since it is not a nutritious substrate for any microorganism, or it was unknown for which one. Currently, it has been reported that beetle larvae of the Tenebrionidae family can biodegrade it thanks to enzymes produced by their intestinal microbiota. Considering the above, the objective of this research was to characterize the intestinal microbiota of Zophobas morio larvae as an approach to the potential use for the biodegradation of Polystyrene, using metabarcoding. For this, the Illumina Miseq sequence of the V3-4 region of the 16s rRNA gene of Z. morio larvae fed with two types of diets, PE and Oats, was performed. Additionally, through the Hiseq2500 sequencing platform, the complete metagenome of one of the samples fed with EPS was obtained. With the results of the sequencing, the taxonomic composition, the relative abundance of the bacteria was analyzed and finally a metabolic analysis of the microbiome obtained from the larvae fed with EPS was carried out. The metagenomic analysis allowed us to identify that the genus Spiroplasma presented a relative abundance of 61.6%, followed by Pantoea (19.3%), Enterobacter (5%) and Pseudarthrobacter (4.7%). Other genera present were Kluyvera georgiana, Enterococcus sp., Bacillus sp., Clostridium sp., and Romboutsia sp. These genera are assumed to be the most promising microorganisms that influence the biodegradation of EPS. The results obtained could be included in future works where the biological activity and biodegradation of microorganisms belonging to the found genera are analyzed. Keywords: Polystyrene, EPS, metagenomics, metabarcoding, biodegradation, Coleoptera.Convocatoria de la Universidad Nacional de Colombia sede Palmira por medio de la plataforma Hermes.contiene material didáctico, ilustraciones y tablasMaestríaMagíster en Ingeniería - Ingeniería AmbientalExtracción de ADN total. Secuenciación masiva empleando amplificaciones y genomas completos. Análisis bioinformáticos. Composición taxonómica y abundancia relativa de bacterias. Análisis metabólico del microbioma obtenido del Metagenoma del tracto digestivo de larvas de Z. morio alimentadas con poliestireno expandido. Control de calidad de las secuencias y remoción de artefactos. Extracción de características. Anotación de características. Filtrado de proteínas, Agrupando AA, Identificación de proteínas, Agrupación de ARNr, Identificación de ARNr, Generación de perfiles.Ciencias omicas y BioinformaticaIngeniería.Sede Palmiraxv, 75 + anexosapplication/pdfspaUniversidad Nacional de ColombiaPalmira - Ingeniería y Administración - Maestría en Ingeniería - Ingeniería AmbientalFacultad de Ingeniería y AdministraciónPalmira, Valle del Cauca, ColombiaUniversidad Nacional de Colombia - Sede Palmira570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animalesMicrobiomasMicrobiomesDiversidad microbianaMicrobial diversityActividad enzimáticaEnzyme activityTenebrionidaeEPSMetabarcodingPoliestirenoMetagenómicaBiodegradaciónColeópterosEPSMetabarcodingColeopteraMetagenomicsBiodegradationPolystyreneEstudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno.Study of the intestinal microbiome of Zophobas morio (Coleoptera: Tenebrionidae) as an approach to the potential use for the biodegradation of polystyrene.Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAhmed, T., Shahid, M., Azeem, F., Rasul, I., Shah, A. 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BioMed Research International, 2018. https://doi.org/10.1155/2018/6765438Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestirenoUniversidad Nacional de ColombiaEstudiantesInvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85544/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1144152058.2023.pdf1144152058.2023.pdfTesis de Maestría en Ingeniería Ambientalapplication/pdf3132369https://repositorio.unal.edu.co/bitstream/unal/85544/2/1144152058.2023.pdf9576b77e8193ec87994cee05e7d93f18MD52THUMBNAIL1144152058.2023.pdf.jpg1144152058.2023.pdf.jpgGenerated Thumbnailimage/jpeg5357https://repositorio.unal.edu.co/bitstream/unal/85544/3/1144152058.2023.pdf.jpg69ff2a6f4cbc87710a3c414b1bb7ee65MD53unal/85544oai:repositorio.unal.edu.co:unal/855442024-08-21 23:14:16.184Repositorio Institucional Universidad Nacional de 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Estudio del microbioma intestinal de Zophobas morio (Coleóptera: Tenebrionidae) como aproximación al uso potencial para la biodegradación del poliestireno.

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