Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting
Background. The immune response of insects involves humoral factors and cellular elements known as hemocytes. There are different reports that explore the response of hemocytes to infections, but the effect that molting has on this response has not been explored so far. We hypothesized that there wo...
- Autores:
-
Ruiz, Elizabeth
López, Myriam Consuelo
Rivas, Favio Aurelio
Sánchez, Ángel Yovanny
Moncada, Ligia Inés
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2015
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/65354
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/65354
http://bdigital.unal.edu.co/66377/
- Palabra clave:
- 61 Ciencias médicas; Medicina / Medicine and health
Rhodnius prolixus
hemocytes
molt
Rhodnius robustus
Rhodnius prolixus
hemocitos
muda
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting |
| title |
Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting |
| spellingShingle |
Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting 61 Ciencias médicas; Medicina / Medicine and health Rhodnius prolixus hemocytes molt Rhodnius robustus Rhodnius prolixus hemocitos muda |
| title_short |
Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting |
| title_full |
Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting |
| title_fullStr |
Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting |
| title_full_unstemmed |
Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting |
| title_sort |
Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting |
| dc.creator.fl_str_mv |
Ruiz, Elizabeth López, Myriam Consuelo Rivas, Favio Aurelio Sánchez, Ángel Yovanny Moncada, Ligia Inés |
| dc.contributor.author.spa.fl_str_mv |
Ruiz, Elizabeth López, Myriam Consuelo Rivas, Favio Aurelio Sánchez, Ángel Yovanny Moncada, Ligia Inés |
| dc.subject.ddc.spa.fl_str_mv |
61 Ciencias médicas; Medicina / Medicine and health |
| topic |
61 Ciencias médicas; Medicina / Medicine and health Rhodnius prolixus hemocytes molt Rhodnius robustus Rhodnius prolixus hemocitos muda |
| dc.subject.proposal.spa.fl_str_mv |
Rhodnius prolixus hemocytes molt Rhodnius robustus Rhodnius prolixus hemocitos muda |
| description |
Background. The immune response of insects involves humoral factors and cellular elements known as hemocytes. There are different reports that explore the response of hemocytes to infections, but the effect that molting has on this response has not been explored so far. We hypothesized that there would be a change in the percentage of hemocytes as a response to the molting process.Objective. The aim of this work was to compare the hemogram (CBC), the formula, and the differential count of hemocytes in IV instar before molting and in V instar nymphs 24 hours after molting in two species: Rhodnius prolixus and Rhodnius robustus.Materials and methods. We assayed different staining methodologies including Giemsa, Alcian Blue pH 2.0, Alcian Blue pH 2.6, Gomori substrate, PAS (Schiff), Sudan Black and Papanicolau with positive controls for each one. In the Gomori staining, we observed lysosomes in the granulocytes and plasmatocytes, but the differentiation was better detected using Giemsa staining.Results. There were no statistically significant differences between the two species studied in plasmatocytes (p=0,053) or even in granulocytes (p=0,5). However, differences were significant in the prohemocytes (p=0,001) during the molting process in both Rhodnius prolixus and Rhodnius robustus.Conclusions. Significant differences in prohemocytes between nymphs of IV and V instar were detected. No significant differences in the amount of cells were observed between the two species and the two stages. These findings may be explained due to their role as precursor cell of prohemocytes. |
| publishDate |
2015 |
| dc.date.issued.spa.fl_str_mv |
2015-01-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-07-03T00:13:13Z |
| dc.date.available.spa.fl_str_mv |
2019-07-03T00:13:13Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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http://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
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ISSN: 2357-3848 |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/65354 |
| dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/66377/ |
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ISSN: 2357-3848 |
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https://repositorio.unal.edu.co/handle/unal/65354 http://bdigital.unal.edu.co/66377/ |
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spa |
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spa |
| dc.relation.spa.fl_str_mv |
https://revistas.unal.edu.co/index.php/revfacmed/article/view/44901 |
| dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Revista de la Facultad de Medicina Revista de la Facultad de Medicina |
| dc.relation.references.spa.fl_str_mv |
Ruiz, Elizabeth and López, Myriam Consuelo and Rivas, Favio Aurelio and Sánchez, Ángel Yovanny and Moncada, Ligia Inés (2015) Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting. Revista de la Facultad de Medicina, 63 (1). pp. 11-17. ISSN 2357-3848 |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Universidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina |
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Universidad Nacional de Colombia |
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https://repositorio.unal.edu.co/bitstream/unal/65354/1/44901-246878-1-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/65354/2/44901-246878-1-PB.pdf.jpg |
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1814090184373829632 |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ruiz, Elizabeth4ce8c521-5930-441d-81cd-7df8b0922386300López, Myriam Consuelodefbb3e9-2dd9-4028-a35f-7b9d45f0d7e8300Rivas, Favio Aurelio5a2d5498-bed5-45de-84eb-dffbebfac26f300Sánchez, Ángel Yovannyd00a4cb3-5ea0-45da-992e-20a47263b45b300Moncada, Ligia Inéscb8bc10d-b25e-4c21-a89d-c964c41a2b743002019-07-03T00:13:13Z2019-07-03T00:13:13Z2015-01-01ISSN: 2357-3848https://repositorio.unal.edu.co/handle/unal/65354http://bdigital.unal.edu.co/66377/Background. The immune response of insects involves humoral factors and cellular elements known as hemocytes. There are different reports that explore the response of hemocytes to infections, but the effect that molting has on this response has not been explored so far. We hypothesized that there would be a change in the percentage of hemocytes as a response to the molting process.Objective. The aim of this work was to compare the hemogram (CBC), the formula, and the differential count of hemocytes in IV instar before molting and in V instar nymphs 24 hours after molting in two species: Rhodnius prolixus and Rhodnius robustus.Materials and methods. We assayed different staining methodologies including Giemsa, Alcian Blue pH 2.0, Alcian Blue pH 2.6, Gomori substrate, PAS (Schiff), Sudan Black and Papanicolau with positive controls for each one. In the Gomori staining, we observed lysosomes in the granulocytes and plasmatocytes, but the differentiation was better detected using Giemsa staining.Results. There were no statistically significant differences between the two species studied in plasmatocytes (p=0,053) or even in granulocytes (p=0,5). However, differences were significant in the prohemocytes (p=0,001) during the molting process in both Rhodnius prolixus and Rhodnius robustus.Conclusions. Significant differences in prohemocytes between nymphs of IV and V instar were detected. No significant differences in the amount of cells were observed between the two species and the two stages. These findings may be explained due to their role as precursor cell of prohemocytes.Antecedentes. La respuesta inmune de los insectos involucra factores humorales y elementos celulares llamados hemocitos. Existen varios trabajos explorando la respuesta de los hemocitos frente a infecciones, pero no como respuesta al proceso de muda.Objetivo. Comparar el hemograma: la fórmula y el recuento diferencial de hemocitos de Rhodnius prolixus y Rhodnius robustus en ninfas de IV estadio antes de la muda y ninfas de V estadio 24 horas después de la muda.Materiales y métodos. Se ensayaron las coloraciones Giemsa, Alcian Blue pH 2.0, Alcian Blue pH 2.6, sustrato Gomori, PAS (Schiff), Sudán Negro, Papanicolau, con controles positivos para cada una. Con la coloración de Gomori se observaron lisosomas en los granulocitos y en plasmatocitos, pero la diferenciación se logró mejor con la coloración de Giemsa.Resultados. Los plasmatocitos fueron más abundantes en las ninfas de IV estadio de Rhodnius robustus y en las de V estadio de las dos especies, pero en las ninfas de V estadio de R. prolixus, el recuento de prohemocitos fue mayor; para los oenocitoides, esferulocitos y adipohemocitos el recuento no alcanzó al 1 %. En las dos especies no se encontraron diferencias estadísticamente significativas en plasmatocitos (p=0,05319), ni en granulocitos (p=0,5), entre las ninfas de IV y V estadio, pero fue significativa en prohemocitos (p=0,001).Conclusiones. Se detectaron diferencias significativas en prohemocitos entre las ninfas de IV y V estadio. No hubo un aumento significativo en el conteo de células en los diferentes estadios.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Medicinahttps://revistas.unal.edu.co/index.php/revfacmed/article/view/44901Universidad Nacional de Colombia Revistas electrónicas UN Revista de la Facultad de MedicinaRevista de la Facultad de MedicinaRuiz, Elizabeth and López, Myriam Consuelo and Rivas, Favio Aurelio and Sánchez, Ángel Yovanny and Moncada, Ligia Inés (2015) Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting. Revista de la Facultad de Medicina, 63 (1). pp. 11-17. ISSN 2357-384861 Ciencias médicas; Medicina / Medicine and healthRhodnius prolixushemocytesmoltRhodnius robustusRhodnius prolixushemocitosmudaComparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after moltingArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL44901-246878-1-PB.pdfapplication/pdf361111https://repositorio.unal.edu.co/bitstream/unal/65354/1/44901-246878-1-PB.pdfad64d48efb16d18359eeb7f6fdabba71MD51THUMBNAIL44901-246878-1-PB.pdf.jpg44901-246878-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8228https://repositorio.unal.edu.co/bitstream/unal/65354/2/44901-246878-1-PB.pdf.jpgf58281d13b0014f8055890849ce63d02MD52unal/65354oai:repositorio.unal.edu.co:unal/653542024-05-10 23:08:35.328Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |