Velocidad y tiempo de centrifugación para extraer nematodos fitoparásitos del suelo.
An experiment was carried out in order to compare different combination of speeds and times of centrifugation to isolate Helicotylenchus dihystera, Meloidogyne incognita and Tylenchulus semipenetrans. The centrifugation-sugar screening method was used. Two loam soils were use with natural infestatio...
- Autores:
-
Volcy, Charles
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 1980
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/39570
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/39570
http://bdigital.unal.edu.co/29667/
- Palabra clave:
- Nematodes
Centrifugation.
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
| Summary: | An experiment was carried out in order to compare different combination of speeds and times of centrifugation to isolate Helicotylenchus dihystera, Meloidogyne incognita and Tylenchulus semipenetrans. The centrifugation-sugar screening method was used. Two loam soils were use with natural infestations of the above mentioned nematodes and a silty loam with natural infestation of H. dihystera. It was concluded that the combination with more consistent results to isolate the spiral eelworm was the centrifugation at 1000 rpm (200 g) for 5 minutes in both cycles, while the combinations with best result to extract the other two species was the centrifugation at 2000 rpm (800 g) for 3 minutes in both cycles. |
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