Actividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10

ilustraciones

Autores:: Ricaurte Contreras, Laura Alejandra

Tipo de recurso:

Fecha de publicación:: 2020

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Actividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10
dc.title.translated.eng.fl_str_mv	Reticulocyte binding activity humans from the preserved fragment of the PvMSP10 protein
title	Actividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10
spellingShingle	Actividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10 500 - Ciencias naturales y matemáticas::507 - Educación, investigación, temas relacionados Inmunología Immunology Plasmodium vivax Malaria Plasmodium vivax Invasión Interacciones proteína-proteína PvMSP10 Reticulocitos humanos Plasmodium vivax Invasion Protein-protein interactions PvMSP10 Human reticulocytes
title_short	Actividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10
title_full	Actividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10
title_fullStr	Actividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10
title_full_unstemmed	Actividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10
title_sort	Actividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10
dc.creator.fl_str_mv	Ricaurte Contreras, Laura Alejandra
dc.contributor.advisor.none.fl_str_mv	Moreno Pérez, Darwin Andrés Patarroyo Gutiérrez, Manuel Alfonso
dc.contributor.author.none.fl_str_mv	Ricaurte Contreras, Laura Alejandra
dc.contributor.researchgroup.spa.fl_str_mv	Biología Molecular de virus
dc.subject.ddc.spa.fl_str_mv	500 - Ciencias naturales y matemáticas::507 - Educación, investigación, temas relacionados
topic	500 - Ciencias naturales y matemáticas::507 - Educación, investigación, temas relacionados Inmunología Immunology Plasmodium vivax Malaria Plasmodium vivax Invasión Interacciones proteína-proteína PvMSP10 Reticulocitos humanos Plasmodium vivax Invasion Protein-protein interactions PvMSP10 Human reticulocytes
dc.subject.decs.none.fl_str_mv	Inmunología Immunology Plasmodium vivax Malaria
dc.subject.proposal.spa.fl_str_mv	Plasmodium vivax Invasión Interacciones proteína-proteína PvMSP10 Reticulocitos humanos
dc.subject.proposal.eng.fl_str_mv	Plasmodium vivax Invasion Protein-protein interactions PvMSP10 Human reticulocytes
description	ilustraciones
publishDate	2020
dc.date.issued.none.fl_str_mv	2020
dc.date.accessioned.none.fl_str_mv	2021-06-24T17:53:27Z
dc.date.available.none.fl_str_mv	2021-06-24T17:53:27Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/79710
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/79710 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.rights.spa.fl_str_mv	Derechos Reservados al Autor, 2020
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dc.format.extent.spa.fl_str_mv	82 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias - Maestría en Ciencias - Microbiología
dc.publisher.department.spa.fl_str_mv	Instituto de Biotecnología (IBUN)
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 InternacionalDerechos Reservados al Autor, 2020http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Moreno Pérez, Darwin Andrés2544f79cefd410a354e61644479f5435Patarroyo Gutiérrez, Manuel Alfonso30322a85ae0ad9c1368bae791386bffeRicaurte Contreras, Laura Alejandra38dd5a28348a3202752f6ae1ae82862eBiología Molecular de virus2021-06-24T17:53:27Z2021-06-24T17:53:27Z2020https://repositorio.unal.edu.co/handle/unal/79710Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustracionesPlasmodium vivax es una de las especies que causa malaria en humanos con mayor importancia epidemiológica en el mundo dada su amplia distribución geográfica. Esta especie es difícil de propagar in vitro dada su preferencia por invadir reticulocitos humanos y por ende su estudio biológico es complicado. Durante la invasión de estos parásitos a sus células diana se presenta un contacto inicial mediado principalmente por moléculas de superficie las cuales establecen diferentes interacciones proteína-proteína. Sin embargo, en P. vivax únicamente se conocen dos proteínas de superficie de merozoitos relacionadas con la adhesión específica a los reticulocitos humanos: MSP1 y RBSA. Dada la importancia de saber qué otras proteínas del parásito participan en el contacto inicial con el hospedero, en este estudio se evaluó, por primera vez, la capacidad de unión de la proteína de superficie de merozoito 10 (PvMSP10) a reticulocitos de humanos adultos. De particular interés, se encontró que PvMSP10 se une a reticulocitos humanos a través de la región C-terminal, cuyo polimorfismo es muy bajo. Además, mediante un ensayo de competición proteína-célula, se determinó que dicha interacción se encuentra gobernada por dos regiones de 20 residuos de longitud (388DKEECRCRANYMPDDSVDYF407 y 415KDCSKENGNCDVNAECSIDK434), que hacen parte de los dominios similares a EGF de esta proteína. Estos hallazgos destacan la importancia de dichos fragmentos para estudiar su utilidad en una futura vacuna contra la malaria causada por P. vivax. (Texto tomado de la fuente)Plasmodium vivax is one of the species that causes malaria in humans with the greatest epidemiological importance in the world given its wide geographical distribution. This species is difficult to propagate in vitro given its preference for invading human reticulocytes and therefore its biological study is complicated. During the invasion of these parasites to their target cells, an initial contact occurs mainly mediated by surface molecules which establish different protein-protein interactions. However, only two merozoite surface proteins related to specific adhesion to human reticulocytes are known in P. vivax: MSP1 and RBSA. Given the importance of knowing which other parasite proteins are involved in the initial contact with the host, this study evaluated for the first time, the binding activity of merozoite surface protein 10 (PvMSP10) to adult human reticulocytes. It was found that PvMSP10 binds to human reticulocytes through the C-terminal region, whose polymorphism is low. Furthermore, by a protein-cell competition assay, it was determined that such interaction is governed by two regions of 20 residues in length (388DKEECRCRANYMPDDSVDYF407 and 415KDCSKENGNCDVNAECSIDK434), which are part of the EGF-like domains. These findings highlight the importance of these fragments to study their usefulness in a future vaccine against malaria caused by P. vivax. (Texto tomado de la fuente)MaestríaMagíster en Ciencias - MicrobiologíaBiología Molecular de Agentes Infecciosos82 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaInstituto de Biotecnología (IBUN)Facultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá500 - Ciencias naturales y matemáticas::507 - Educación, investigación, temas relacionadosInmunologíaImmunologyPlasmodium vivaxMalariaPlasmodium vivaxInvasiónInteracciones proteína-proteínaPvMSP10Reticulocitos humanosPlasmodium vivaxInvasionProtein-protein interactionsPvMSP10Human reticulocytesActividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10Reticulocyte binding activity humans from the preserved fragment of the PvMSP10 proteinTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAikawa, M., Miller, L. 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J Infect Dis, 211(7), 1111-1120. doi:10.1093/infdis/jiu558GeneralFundación Instituto Inmunología de ColombiaLICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/79710/1/license.txtcccfe52f796b7c63423298c2d3365fc6MD51ORIGINAL1030657558.2020.pdf1030657558.2020.pdfTesis de Maestría en Microbiologíaapplication/pdf5844457https://repositorio.unal.edu.co/bitstream/unal/79710/2/1030657558.2020.pdfecfa0168e33988c0535e3422bb2bbf88MD52THUMBNAIL1030657558.2020.pdf.jpg1030657558.2020.pdf.jpgGenerated Thumbnailimage/jpeg5038https://repositorio.unal.edu.co/bitstream/unal/79710/3/1030657558.2020.pdf.jpg97c7543418bbcf00eba05fbae9a6d5cbMD53unal/79710oai:repositorio.unal.edu.co:unal/797102024-07-23 23:33:14.812Repositorio Institucional Universidad Nacional de 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Actividad de unión a reticulocitos humanos del fragmento conservado de la proteína PvMSP10

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