Estudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina cruda

ilustraciones, fotografías, graficas

Autores:: Aragón Caycedo, Oscar Leonardo

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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dc.title.spa.fl_str_mv	Estudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina cruda
dc.title.translated.eng.fl_str_mv	Study of a bio-electrochemical fermentation system to produce 1,3-propanediol from crude glycerin
title	Estudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina cruda
spellingShingle	Estudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina cruda 660 - Ingeniería química::661 - Tecnología de químicos industriales 570 - Biología::572 - Bioquímica FERMENTACION GLICERINA-ANALISIS Fermentation Glycerin - analysis Glicerol 1,3-propanodiol Fermentacion Clostridium Electrofermentacion Hidrógeno Cátodo Análisis de modo elemental Clostridium butyricum Modelo metabólico central Glycerol 1,3-propanediol Fermentation Clostridium Electrofermentation Hydrogen Cathode Elementary mode analysis Central metabolic model Propano-1,3-diol
title_short	Estudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina cruda
title_full	Estudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina cruda
title_fullStr	Estudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina cruda
title_full_unstemmed	Estudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina cruda
title_sort	Estudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina cruda
dc.creator.fl_str_mv	Aragón Caycedo, Oscar Leonardo
dc.contributor.advisor.none.fl_str_mv	Montoya Castaño, Dolly
dc.contributor.author.none.fl_str_mv	Aragón Caycedo, Oscar Leonardo
dc.contributor.researchgroup.spa.fl_str_mv	Bioprocesos y Bioprospeccion
dc.contributor.orcid.spa.fl_str_mv	OSCAR LEONARDO ARAGON CAICEDO [0000000193632258]
dc.contributor.researchgate.spa.fl_str_mv	https://www.researchgate.net/profile/Oscar-Aragon
dc.subject.ddc.spa.fl_str_mv	660 - Ingeniería química::661 - Tecnología de químicos industriales 570 - Biología::572 - Bioquímica
topic	660 - Ingeniería química::661 - Tecnología de químicos industriales 570 - Biología::572 - Bioquímica FERMENTACION GLICERINA-ANALISIS Fermentation Glycerin - analysis Glicerol 1,3-propanodiol Fermentacion Clostridium Electrofermentacion Hidrógeno Cátodo Análisis de modo elemental Clostridium butyricum Modelo metabólico central Glycerol 1,3-propanediol Fermentation Clostridium Electrofermentation Hydrogen Cathode Elementary mode analysis Central metabolic model Propano-1,3-diol
dc.subject.lemb.spa.fl_str_mv	FERMENTACION GLICERINA-ANALISIS
dc.subject.lemb.eng.fl_str_mv	Fermentation Glycerin - analysis
dc.subject.proposal.spa.fl_str_mv	Glicerol 1,3-propanodiol Fermentacion Clostridium Electrofermentacion Hidrógeno Cátodo Análisis de modo elemental Clostridium butyricum Modelo metabólico central
dc.subject.proposal.eng.fl_str_mv	Glycerol 1,3-propanediol Fermentation Clostridium Electrofermentation Hydrogen Cathode Elementary mode analysis Central metabolic model
dc.subject.wikidata.spa.fl_str_mv	Propano-1,3-diol
description	ilustraciones, fotografías, graficas
publishDate	2023
dc.date.accessioned.none.fl_str_mv	2023-07-19T14:02:51Z
dc.date.available.none.fl_str_mv	2023-07-19T14:02:51Z
dc.date.issued.none.fl_str_mv	8-07-23
dc.type.spa.fl_str_mv	Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TD
format	http://purl.org/coar/resource_type/c_db06
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/84216
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co
url	https://repositorio.unal.edu.co/handle/unal/84216 https://repositorio.unal.edu.co
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
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dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Montoya Castaño, Dolly0a1f1a23603bd38431d54a1a88c0b760Aragón Caycedo, Oscar Leonardo7fd2b2d2c4a11d74fd7cef59006d4237Bioprocesos y BioprospeccionOSCAR LEONARDO ARAGON CAICEDO [0000000193632258]https://www.researchgate.net/profile/Oscar-Aragon2023-07-19T14:02:51Z2023-07-19T14:02:51Z8-07-23https://repositorio.unal.edu.co/handle/unal/84216Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.coilustraciones, fotografías, graficasLos azúcares y el glicerol pueden servir como sustratos de bajo costo en aplicaciones biotecnológicas para obtener varios intermediarios químicos con un alto valor agregado. La electrofermentación es una reciente tecnología con la que es posible mejorar y controlar la fermentación microbiana, especialmente con cepas del género Clostridium, aumentando la especificidad de las vías metabólicas. En este contexto, cepas bacterianas aisladas de suelos colombianos, y estrechamente relacionadas con Clostridium butyricum, se han identificado como eficientes productoras de solventes y ácidos, incluidos ácido acético, ácido butírico, etanol, butanol, acetona e hidrógeno a partir de glucosa o 1,3-propanodiol a partir de glicerol. En este trabajo se evalúa el efecto del suministro externo de electrones en la producción de metabolitos de interés comercial con una red metabólica de C. butyricum. Los resultados obtenidos de un modelo de simulación señalan que la interacción con el electrodo catódico mejora los rendimientos de productos reducidos. En concreto, utilizando glicerol como sustrato, la simulación indicó que el rendimiento medio del producto podría aumentar con 1,3-propanodiol (23%) e hidrógeno (45%). Por último, se estableció experimentalmente que la cepa nativa IBUN 158B es electroactiva y tiene la capacidad de incrementar los valores de rendimiento producto / sustrato de 1,3-PD (7 – 9%) cuando es sometida a la alimentación de pequeñas cantidades de electrones desde un cátodo en un proceso electrofermentación catódica y que el uso de transportadores de electrones como el Rojo Neutral incrementa los efectos de la electrofermentación alcanzando mayores valores de rendimiento cuando está presente en el medio de cultivo. En conclusión, la electrofermentación de Clostridium butyricum como técnica de cultivo bioelectroquímico tiene potencial como proceso de producción alternativo a la fermentación tradicional para controlar el estado redox durante la síntesis de bioquímicos y aumentar la producción de metabolitos de interés comercial. Pero se necesita más investigación básica y aplicada para dilucidar los mecanismos de transferencia de electrones y revelar los mecanismos reguladores subyacentes. (Texto tomado de la fuente)Sugars and glycerol can serve as low-cost substrates in biotechnological applications to obtain various chemical intermediates with high added value. Electrofermentation is a recent technology with which it is possible to improve and control microbial fermentation, especially with strains of the Clostridium genus, increasing the specificity of metabolic pathways. In this context, bacterial strains isolated from Colombian soils, and closely related to Clostridium butyricum. These strains have been efficient producers of solvents and acids, including acetic acid, butyric acid, ethanol, butanol, acetone, and hydrogen from glucose or 1,3-propanediol from glycerol. In this work, the production of commercial interest metabolites is assessed using an electron external supply with a metabolic network of C. butyricum. The simulation results show that the interaction with the cathode electrode improves the reduced product rates. Specifically, using glycerol as a substrate, the average yield of the product increases with 1,3-propanediol (23%) and hydrogen (45%). Finally, it was established experimentally that the native strain IBUN 158B is electroactive and has the capacity to increase the product/substrate yield values of 1,3-PD (7-9%) when it is submitted to the feeding of small amounts of electrons from a cathode in a cathodic electrofermentation process and that the use of electron carriers such as Neutral Red increases the effects of electrofermentation, reaching higher yield values when it is present in the culture medium. In conclusion, the electrofermentation of Clostridium butyricum as a bioelectrochemical culture technique has potential as an alternative production process to traditional fermentation to control the redox state during the synthesis of biochemicals and increase the production of metabolites of commercial interest. More basic and applied research is necessary to elucidate the mechanisms of electron transfer and reveal the underlying regulatory mechanisms.DoctoradoDoctorado en BiotecnologíaBioprocesosMicroorganismos solventogénicosxvi, 113 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Doctorado en BiotecnologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá660 - Ingeniería química::661 - Tecnología de químicos industriales570 - Biología::572 - BioquímicaFERMENTACIONGLICERINA-ANALISISFermentationGlycerin - analysisGlicerol1,3-propanodiolFermentacionClostridiumElectrofermentacionHidrógenoCátodoAnálisis de modo elementalClostridium butyricumModelo metabólico centralGlycerol1,3-propanediolFermentationClostridiumElectrofermentationHydrogenCathodeElementary mode analysisCentral metabolic modelPropano-1,3-diolEstudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina crudaStudy of a bio-electrochemical fermentation system to produce 1,3-propanediol from crude glycerinTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDVees CA, Neuendorf CS, Pflügl S (2020) Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives, Springer International Publishing.Dahiya S, Katakojwala R, Ramakrishna S, et al. 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Curr Opin Biotechnol 23: 364–381ColcienciasInvestigadoresORIGINAL79788352 2023.pdf79788352 2023.pdfTesis de Doctorado en Biotecnologíaapplication/pdf2225809https://repositorio.unal.edu.co/bitstream/unal/84216/2/79788352%202023.pdf0897b27ba2e373ee08be0984b4fead97MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84216/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53THUMBNAIL79788352 2023.pdf.jpg79788352 2023.pdf.jpgGenerated Thumbnailimage/jpeg5516https://repositorio.unal.edu.co/bitstream/unal/84216/4/79788352%202023.pdf.jpg21effa7d87f108d86d11a689ea194747MD54unal/84216oai:repositorio.unal.edu.co:unal/842162024-08-14 23:41:22.523Repositorio Institucional Universidad Nacional de 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Estudio de un sistema bio-electroquímico de fermentación para la producción de 1,3-propanodiol a partir de glicerina cruda

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