Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties

Spodoptera frugiperda is one of the most common pests in maize crops, causing important losses in Colombia. The development of insect resistant crops is an alternative to conventional pest control methods. HERCULEX® I is a transgenic maize line with resistance to lepidopteran insects and tolerance t...

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Autores:
Jiménez-Barreto, Jenny
Chaparro Giraldo, Alejandro
Mora-Oberlaender, Julián
Vargas-Sánchez, José Ever
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/61412
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/61412
http://bdigital.unal.edu.co/60222/
Palabra clave:
63 Agricultura y tecnologías relacionadas / Agriculture
crop improvement
PCR
pest resistance.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Jiménez-Barreto, Jenny461e51b8-5a51-4deb-be24-843ecb3c8b8c300Chaparro Giraldo, Alejandroa3360dfb-f379-46e5-8592-e684e681f1b2300Mora-Oberlaender, Julián78875983-b896-40a0-bd52-7c4b32b33264300Vargas-Sánchez, José Ever26fca472-5c8b-4de4-bae8-2f86178fd3f03002019-07-02T20:42:01Z2019-07-02T20:42:01Z2016-09-01ISSN: 2357-3732https://repositorio.unal.edu.co/handle/unal/61412http://bdigital.unal.edu.co/60222/Spodoptera frugiperda is one of the most common pests in maize crops, causing important losses in Colombia. The development of insect resistant crops is an alternative to conventional pest control methods. HERCULEX® I is a transgenic maize line with resistance to lepidopteran insects and tolerance to phosphinothricin herbicides, conferred by genes cry1F and pat, respectively. In Colombia, the National Federation of Cereal and Legume Growers (FENALCE) has carried out mendelian crosses between Colombian maize genotypes and HERCU- LEX® I, with the aim of integrating the genetic elements of the transgenic line into national varieties. Three hybrids were obtained which potentially carry such constructs. In the present study, the hybrid lines and their parental lines were characterized at the molecular level. Leaf tissue was tested for presence or absence of the genes cry1F and pat and their expression as mRNA and respective proteins Cry1F and Pat. Results show that the three hybrids indeed carry the HERCULEX® I constructs. RT-PCR and ELISA analysis showed transcription of the genes and presence of the proteins. An initial approach to the Freedom to Operate analysis was carried out for HERCU- LEX® I in Colombia.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrariashttps://revistas.unal.edu.co/index.php/agrocol/article/view/60350Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaJiménez-Barreto, Jenny and Chaparro Giraldo, Alejandro and Mora-Oberlaender, Julián and Vargas-Sánchez, José Ever (2016) Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties. Agronomía Colombiana, 34 (3). pp. 309-316. ISSN 2357-373263 Agricultura y tecnologías relacionadas / Agriculturecrop improvementPCRpest resistance.Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varietiesArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL60350-322500-1-PB.pdfapplication/pdf216157https://repositorio.unal.edu.co/bitstream/unal/61412/1/60350-322500-1-PB.pdf2122fda4f1e2b7388934356441a8beaeMD51THUMBNAIL60350-322500-1-PB.pdf.jpg60350-322500-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg7854https://repositorio.unal.edu.co/bitstream/unal/61412/2/60350-322500-1-PB.pdf.jpga601747dabf7a3c6216c0723028dedf6MD52unal/61412oai:repositorio.unal.edu.co:unal/614122024-04-18 23:51:23.718Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties
title Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties
spellingShingle Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties
63 Agricultura y tecnologías relacionadas / Agriculture
crop improvement
PCR
pest resistance.
title_short Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties
title_full Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties
title_fullStr Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties
title_full_unstemmed Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties
title_sort Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties
dc.creator.fl_str_mv Jiménez-Barreto, Jenny
Chaparro Giraldo, Alejandro
Mora-Oberlaender, Julián
Vargas-Sánchez, José Ever
dc.contributor.author.spa.fl_str_mv Jiménez-Barreto, Jenny
Chaparro Giraldo, Alejandro
Mora-Oberlaender, Julián
Vargas-Sánchez, José Ever
dc.subject.ddc.spa.fl_str_mv 63 Agricultura y tecnologías relacionadas / Agriculture
topic 63 Agricultura y tecnologías relacionadas / Agriculture
crop improvement
PCR
pest resistance.
dc.subject.proposal.spa.fl_str_mv crop improvement
PCR
pest resistance.
description Spodoptera frugiperda is one of the most common pests in maize crops, causing important losses in Colombia. The development of insect resistant crops is an alternative to conventional pest control methods. HERCULEX® I is a transgenic maize line with resistance to lepidopteran insects and tolerance to phosphinothricin herbicides, conferred by genes cry1F and pat, respectively. In Colombia, the National Federation of Cereal and Legume Growers (FENALCE) has carried out mendelian crosses between Colombian maize genotypes and HERCU- LEX® I, with the aim of integrating the genetic elements of the transgenic line into national varieties. Three hybrids were obtained which potentially carry such constructs. In the present study, the hybrid lines and their parental lines were characterized at the molecular level. Leaf tissue was tested for presence or absence of the genes cry1F and pat and their expression as mRNA and respective proteins Cry1F and Pat. Results show that the three hybrids indeed carry the HERCULEX® I constructs. RT-PCR and ELISA analysis showed transcription of the genes and presence of the proteins. An initial approach to the Freedom to Operate analysis was carried out for HERCU- LEX® I in Colombia.
publishDate 2016
dc.date.issued.spa.fl_str_mv 2016-09-01
dc.date.accessioned.spa.fl_str_mv 2019-07-02T20:42:01Z
dc.date.available.spa.fl_str_mv 2019-07-02T20:42:01Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.issn.spa.fl_str_mv ISSN: 2357-3732
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identifier_str_mv ISSN: 2357-3732
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dc.language.iso.spa.fl_str_mv spa
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dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/agrocol/article/view/60350
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
Agronomía Colombiana
dc.relation.references.spa.fl_str_mv Jiménez-Barreto, Jenny and Chaparro Giraldo, Alejandro and Mora-Oberlaender, Julián and Vargas-Sánchez, José Ever (2016) Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties. Agronomía Colombiana, 34 (3). pp. 309-316. ISSN 2357-3732
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
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eu_rights_str_mv openAccess
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrarias
institution Universidad Nacional de Colombia
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