Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties

Spodoptera frugiperda is one of the most common pests in maize crops, causing important losses in Colombia. The development of insect resistant crops is an alternative to conventional pest control methods. HERCULEX® I is a transgenic maize line with resistance to lepidopteran insects and tolerance t...

Full description

Autores:
Jiménez-Barreto, Jenny
Chaparro Giraldo, Alejandro
Mora-Oberlaender, Julián
Vargas-Sánchez, José Ever
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/61412
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/61412
http://bdigital.unal.edu.co/60222/
Palabra clave:
63 Agricultura y tecnologías relacionadas / Agriculture
crop improvement
PCR
pest resistance.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
Description
Summary:Spodoptera frugiperda is one of the most common pests in maize crops, causing important losses in Colombia. The development of insect resistant crops is an alternative to conventional pest control methods. HERCULEX® I is a transgenic maize line with resistance to lepidopteran insects and tolerance to phosphinothricin herbicides, conferred by genes cry1F and pat, respectively. In Colombia, the National Federation of Cereal and Legume Growers (FENALCE) has carried out mendelian crosses between Colombian maize genotypes and HERCU- LEX® I, with the aim of integrating the genetic elements of the transgenic line into national varieties. Three hybrids were obtained which potentially carry such constructs. In the present study, the hybrid lines and their parental lines were characterized at the molecular level. Leaf tissue was tested for presence or absence of the genes cry1F and pat and their expression as mRNA and respective proteins Cry1F and Pat. Results show that the three hybrids indeed carry the HERCULEX® I constructs. RT-PCR and ELISA analysis showed transcription of the genes and presence of the proteins. An initial approach to the Freedom to Operate analysis was carried out for HERCU- LEX® I in Colombia.