Optimización de la producción de celulasa a partir de Fusarium sp.

Ilustraciones

Autores:: Bonilla Ospina, Nataly

Tipo de recurso:

Fecha de publicación:: 2020

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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dc.title.spa.fl_str_mv	Optimización de la producción de celulasa a partir de Fusarium sp.
dc.title.translated.none.fl_str_mv	Optimization of cellulase production by Fusarium sp.
title	Optimización de la producción de celulasa a partir de Fusarium sp.
spellingShingle	Optimización de la producción de celulasa a partir de Fusarium sp. 660 - Ingeniería química Celulosa Cellulose Fusarium sp Enzimas Celulasas Endoglucanasa Metodología superficie de respuesta Enzymes Cellulase Endoglucanases Response Surface Methodology
title_short	Optimización de la producción de celulasa a partir de Fusarium sp.
title_full	Optimización de la producción de celulasa a partir de Fusarium sp.
title_fullStr	Optimización de la producción de celulasa a partir de Fusarium sp.
title_full_unstemmed	Optimización de la producción de celulasa a partir de Fusarium sp.
title_sort	Optimización de la producción de celulasa a partir de Fusarium sp.
dc.creator.fl_str_mv	Bonilla Ospina, Nataly
dc.contributor.advisor.none.fl_str_mv	Ruiz-Colorado, Angela Adriana
dc.contributor.author.none.fl_str_mv	Bonilla Ospina, Nataly
dc.contributor.researchgroup.spa.fl_str_mv	Bioprocesos y Flujos Reactivos
dc.subject.ddc.spa.fl_str_mv	660 - Ingeniería química
topic	660 - Ingeniería química Celulosa Cellulose Fusarium sp Enzimas Celulasas Endoglucanasa Metodología superficie de respuesta Enzymes Cellulase Endoglucanases Response Surface Methodology
dc.subject.lemb.none.fl_str_mv	Celulosa Cellulose
dc.subject.proposal.spa.fl_str_mv	Fusarium sp Enzimas Celulasas Endoglucanasa Metodología superficie de respuesta
dc.subject.proposal.eng.fl_str_mv	Enzymes Cellulase Endoglucanases Response Surface Methodology
description	Ilustraciones
publishDate	2020
dc.date.issued.none.fl_str_mv	2020-12-03
dc.date.accessioned.none.fl_str_mv	2022-03-31T19:44:02Z
dc.date.available.none.fl_str_mv	2022-03-31T19:44:02Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
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dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/81428
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/81428 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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A., Tremacoldi, R., … Sanchez Farinas, C. (2013). Effect of initial moisture content on two Amazon rainforest Aspergillus strains cultivated on agro-industrial residues: Biomass-degrading enzymes production and characterization. Industrial Crops and Products, 42, 236–242. https://doi.org/10.1016/j.indcrop.2012.05.035 De Almeida, M. N., Guimarães, V. M., Bischoff, K. M., Falkoski, D. L., Pereira, O. L., Gonçalves, D. S. P. O., & de Rezende, S. T. (2011). Cellulases and hemicellulases from endophytic acremonium species and its application on sugarcane bagasse hydrolysis. Applied Biochemistry and Biotechnology, 165(2), 594–610. https://doi.org/10.1007/s12010-011- 9278-z De Almeida, M. N., Guimarães, V. M., Falkoski, D. L., Paes, G. B. T., Ribeiro, J. I., Visser, E. M., de Rezende, S. T. (2014). Optimization of endoglucanase and xylanase activities from Fusarium verticillioides for simultaneous saccharification and fermentation of sugarcane bagasse. Applied Biochemistry and Biotechnology, 172(3), 1332–1346. https://doi.org/10.1007/s12010-013-0572-9 de Cassia Pereira, J., Paganini Marques, N., Rodrigues, A., Brito de Oliveira, T., Boscolo, M., da Silva, R., … Bocchini Martins, D. A. (2015). Thermophilic fungi as new sources for production of cellulases and xylanases with potential use in sugarcane bagasse saccharification. Journal of Applied Microbiology, 118(4), 928–939. https://doi.org/10.1111/jam.12757 Deswal, D., Khasa, Y. P., & Kuhad, R. C. (2011). Optimization of cellulase production by a brown rot fungus Fomitopsis sp. RCK2010 under solid state fermentation. Bioresource Technology, 102(10), 6065–6072. https://doi.org/10.1016/j.biortech.2011.03.032 Elisashvili, V., Kachlishvili, E., & Penninckx, M. (2008). Effect of growth substrate, method of fermentation, and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes. 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(2004). Modelling of simultaneous effect of moisture and temperature on A. niger growth in solid-state fermentation. Biochemical Engineering Journal, 21(3), 265–272. https://doi.org/10.1016/j.bej.2004.07.007 Jha, K., Khare, S. K., & Gandhi, A. P. (1995). Solid-state fermentation of soyhull for the production of cellulase. Bioresource Technology, 54(3), 321–322. https://doi.org/10.1016/0960-8524(95)00154-9 Juturu, V., & Wu, J. C. (2014). Microbial cellulases: Engineering, production and applications. Renewable and Sustainable Energy Reviews, 33, 188–203. Retrieved from https://www- sciencedirect-com.ezproxy.unal.edu.co/science/article/pii/S1364032114000999 Kachlishvili, E., Penninckx, M. J., Tsiklauri, N., & Elisashvili, V. (2006). Effect of nitrogen source on lignocellulolytic enzyme production by white-rot basidiomycetes under solid-state cultivation. World Journal of Microbiology and Biotechnology, 22(4), 391–397. https://doi.org/10.1007/s11274-005-9046-8 Khan, M. M. H., Ali, S., Fakhru’l-Razi, A., & Alam, M. Z. (2007). Use of fungi for the bioconversion of rice straw into cellulase enzyme. Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, 42(4), 381–386. https://doi.org/10.1080/03601230701312647 Kilikian, B. V, Afonso, L. C., Souza, T. F. C., Ferreira, R. G., & Pinheiro, I. R. (n.d.). Filamentous fungi and media for cellulase production in solid state cultures. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059312/pdf/bjm-45-279.pdf King, B. C., Donnelly, M. K., Bergstrom, G. C., Walker, L. P., & Gibson, D. M. (2009). An optimized microplate assay system for quantitative evaluation of plant cell wall-degrading enzyme activity of fungal culture extracts. Biotechnology and Bioengineering, 102(4), 1033–1044. https://doi.org/10.1002/bit.22151 Kobakhidze, A., Asatiani, M., Kachlishvili, E., & Elisashvili, V. (2016). Induction and catabolite repression of cellulase and xylanase synthesis in the selected white-rot basidiomycetes. Annals of Agrarian Science. https://doi.org/10.1016/j.aasci.2016.07.001 Kumar Bharti, A., Kumar, A., Kumar, A., & Dutt, D. (2018). Exploitation of Parthenium hysterophorous biomass as low-cost substrate for cellulase and xylanase production under solid-state fermentation using Talaromyces stipitatus MTCC 12687. https://doi.org/10.1016/j.jrras.2018.01.003 Kumaran, S., Sastry, C. A., & Vikineswary, S. (1997). Laccase, cellulase and xylanase activities during growth of Pleurotus sajor-caju on sago hampas. World Journal of Microbiology and Biotechnology, 13(1), 43–49. https://doi.org/10.1007/BF02770806 Kobakhidze, A., Asatiani, M., Kachlishvili, E., & Elisashvili, V. (2016). Induction and catabolite repression of cellulase and xylanase synthesis in the selected white-rot basidiomycetes. Annals of Agrarian Science. https://doi.org/10.1016/j.aasci.2016.07.001 Kumar Bharti, A., Kumar, A., Kumar, A., & Dutt, D. (2018). Exploitation of Parthenium hysterophorous biomass as low-cost substrate for cellulase and xylanase production under solid-state fermentation using Talaromyces stipitatus MTCC 12687. https://doi.org/10.1016/j.jrras.2018.01.003 Li, Y., Liu, C., Bai, F., & Zhao, X. (2016). Overproduction of cellulase by Trichoderma reesei RUT C30 through batch-feeding of synthesized low-cost sugar mixture. Bioresource Technology, 216, 503–510. https://doi.org/10.1016/j.biortech.2016.05.108 Lee, C. K., Darah, I., & Ibrahim, C. O. (2011). Production and Optimization of Cellulase Enzyme Using Aspergillus niger USM AI 1 and Comparison with Trichoderma reesei via Solid State Fermentation System. Biotechnology Research International, 2011, 1–6. https://doi.org/10.4061/2011/658493 Liu, X., & Kokare, C. (2017). Chapter 11 – Microbial Enzymes of Use in Industry. In Biotechnology of Microbial Enzymes (pp. 267–298). https://doi.org/10.1016/B978-0-12-803725-6.00011-X Marín, M., Anchez, A. S., & Artola, A. (2019). Production and recovery of cellulases through solid-state fermentation of selected lignocellulosic wastes. https://doi.org/10.1016/j.jclepro.2018.10.264 Marques, N. P., De Cassia Pereira, J., Gomes, E., Da Silva, R., Araújo, A. R., Ferreira, H., … Bocchini, D. A. (2018). Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse T to the production of cellulases and xylanases and their enzymatic extracts have potential for application in pre-treated sugarcane bagasse saccharification processes. https://doi.org/10.1016/j.indcrop.2018.05.022 Nazir, A., Soni, R., Saini, H. S., Kaur, A., & Chadha, B. S. (2010). Profiling differential expression of cellulases and metabolite footprints in aspergillus terreus. 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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Medellín - Minas - Maestría en Ingeniería - Ingeniería Química
dc.publisher.department.spa.fl_str_mv	Departamento de Procesos y Energía
dc.publisher.faculty.spa.fl_str_mv	Facultad de Minas
dc.publisher.place.spa.fl_str_mv	Medellín, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Medellín
institution	Universidad Nacional de Colombia
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ruiz-Colorado, Angela Adriana7cd7a63c83a2319d2d956ac8a78e5cd2600Bonilla Ospina, Natalyd1799e62d4dc17a59283eeecfc3bb8cdBioprocesos y Flujos Reactivos2022-03-31T19:44:02Z2022-03-31T19:44:02Z2020-12-03https://repositorio.unal.edu.co/handle/unal/81428Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/IlustracionesEl uso de enzimas en diferentes sectores industriales es una alternativa a los procesos químicos convencionales. Las enzimas son atractivas, debido a ventajas técnico-económicas en el proceso, como bajas temperaturas (30ºC - 50ºC), pH (4,0 -5,5) y concentraciones de solventes orgánicos, entre otros. Las enzimas, ofrecen alta especificidad al sustrato, condiciones de proceso moderadas a suaves comparadas con procesos industriales convencionales, baja toxicidad y pureza del producto. En consecuencia, reducción de impactos ambientales negativos. Las celulasas, son el segundo tipo de enzimas predominantes en la industria biotecnológica ya que tiene numerosas aplicaciones en diferentes campos, incluyendo la industria textil, pulpa y papel, alimentos, producción de biocombustibles, farmacéutica, entre otras. Su biosíntesis es controlada por mecanismos como la inducción y la regulación nutricional (regulación de las fuentes de carbono o nitrógeno), principalmente. Diferentes microorganismos son capaces de producir el complejo enzimático celulasas, sin embargo, hongos como Fusarium sp. son ampliamente estudiados, por su buen rendimiento en la producción enzimas y su habilidad de secretar el complejo extracelularmente. En este estudio, Carboximetilcelulosa fue usado como única fuente de carbón para la producción de enzimas celulolíticas (celulasas y endoglucanasas), por Fusarium sp. bajo fermentación en estado sólido (FES). Los efectos de la humedad (65% -80%), la temperatura (28-35ºC), el pH (4,5 – 6,0 Unidades) y el tiempo de fermentación (2-6 días) sobre la producción enzimática, fueron determinados siguiendo la metodología de superficie de respuesta. La condición ideal para la producción para Fusarium sp., tanto de celulasas como endoglucanasas fueron 10 días de fermentación, 71,74% de humedad, pH 5,02 y temperatura 28,8 ºC. La síntesis de celulasa fue reprimida en presencia de xilosa y fructosa, mientras que, fue inducida en presencia de lactosa y soforosa. Finalmente, se caracterizó el extracto enzimático a diferentes temperaturas y pH, lo que permitió determinar que la actividad relativa para endoglucanasa se presenta a 50ºC y pH 5,0, mientras que para actividad celulasa, se presenta a 60ºC y pH 6,0. (Texto tomado de la fuente)The use of enzymes in different industrial sectors is a conventional alternative to chemical processes, it is attractive due to technical and economic advantages in the process, such as low temperatures (30ºC - 50ºC), pH (4,0 -5,5) and concentrations of organic solvents, among others. Enzymes provide high substrate specificity, moderate to mild process conditions, low toxicity and product purity, thus reducing negative environmental impacts. Cellulases, the second type of enzymes predominant in the industry, are mainly controlled by mechanisms such as induction and nutritional regulation (regulation of carbon or nitrogen sources). Different microorganisms can produce complex cellulase enzymes; however, fungi like Fusarium sp. are widely studied for their good yield in the production of enzymes and their ability to secrete the complex extracellularly. Carboxymethylcellulose was used as the sole source of carbon to produce cellulolytic enzymes, by Fusarium sp. under solid state fermentation (FES, by its acronym in Spanish). The effects of moisture (65-80%), temperature (28-35ºC), pH (4.5 - 6.0 Units) and fermentation time (2-6 days) on enzymatic production were determined following the surface response methodology. The ideal condition for endoglucanase production was 4 days of fermentation 70% humidity, pH 4.5 and temperature 30 ° C, these factors being of significant influence on Fusarium sp. The cellulase synthesis was repressed in the presence of xylose and fructose, while it was induced in the presence of lactose and sophorose. Finally, the enzyme extract was characterized at different temperatures and pH, which determined the highest relative activity for endoglucanase is presented at 50 °C and pH 5.0, while for cellulase activity, it is presented at 60 °C and pH 6.0.MaestríaMagíster en Ingeniería - Ingeniería QuímicaBioprocesosÁrea curricular de Ingeniería Química e Ingeniería de Petróleos62 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Minas - Maestría en Ingeniería - Ingeniería QuímicaDepartamento de Procesos y EnergíaFacultad de MinasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín660 - Ingeniería químicaCelulosaCelluloseFusarium spEnzimasCelulasasEndoglucanasaMetodología superficie de respuestaEnzymesCellulaseEndoglucanasesResponse Surface MethodologyOptimización de la producción de celulasa a partir de Fusarium sp.Optimization of cellulase production by Fusarium sp.Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAhamed, A., & Vermette, P. 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Bioresource Technology, 101(1), 222–227. https://doi.org/10.1016/j.biortech.2009.08.013.InvestigadoresORIGINAL1107036049.2020.pdf1107036049.2020.pdfTesis Maestría en Ingeniería Químicaapplication/pdf1681803https://repositorio.unal.edu.co/bitstream/unal/81428/5/1107036049.2020.pdf85ac985cd8ac01fab4d8fe9113e823d4MD55LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81428/4/license.txt8153f7789df02f0a4c9e079953658ab2MD54THUMBNAIL1107036049.2020.pdf.jpg1107036049.2020.pdf.jpgGenerated Thumbnailimage/jpeg5561https://repositorio.unal.edu.co/bitstream/unal/81428/6/1107036049.2020.pdf.jpg1d05b7183e80327dbb877cde1929a5d3MD56unal/81428oai:repositorio.unal.edu.co:unal/814282023-10-13 14:12:20.305Repositorio Institucional Universidad Nacional de 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Optimización de la producción de celulasa a partir de Fusarium sp.

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